Epitopes of serogroup B Neisseria meningitidis analysed in vitro and directly from cerebrospinal fluid.

Abstract:

:Two type B15 P1.16 strains of Neisseria meningitidis were examined by immunogold electron microscopy for accessibility of two outer membrane protein (OMPs) to monoclonal antibodies. Both strains exhibited cell-to-cell variation of one epitope of the Class 3 OMP (P3.15) and one of the Class 1 OMPs (P1.16) when grown in vitro. One strain, a nasopharyngeal isolate revealed this variation to be growth-phase independent and double labelling of both epitopes showed independent variation. CSF containing N. meningitidis was stored in liquid nitrogen without laboratory processing at the time of isolation of the second strain. Direct analysis of the organisms showed no cell-to-cell variation and immunoglobulin G on the surface. However, while there were similar labelling densities of the Class 1 epitope in vivo compared with either strain grown in vitro, there was a lower labelling density of the Class 3 epitope in vivo that was not caused by freeze-thawing. This reduction may be due to decreased expression of this epitope in vivo which casts doubts on the use of the Class 2/3 OMP as a vaccine candidate.

journal_name

FEMS Microbiol Lett

authors

Wall RA,Davies HA,Borriello SP

doi

10.1016/0378-1097(89)90379-0

subject

Has Abstract

pub_date

1989-11-01 00:00:00

pages

129-35

issue

1-2

eissn

0378-1097

issn

1574-6968

journal_volume

53

pub_type

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