A surface polysaccharide forms when gonococci are converted to serum resistance by cytidine 5'-monophospho-N-acetyl neuraminic acid.


:A serum-susceptible, guinea-pig chamber-passaged, laboratory strain (BS4 (agar)) of Neisseria gonorrhoeae was converted to serum resistance by incubation with cytidine 5-monophospho-N-acetyl neuraminic acid (CMP-NANA) and examined by electron microscopy after staining with ruthenium-red. In contrast to serum susceptible gonococci incubated without CMP-NANA, the majority (60-70%) of the serum resistant organisms showed a surface accumulation of polysaccharide. This surface polysaccharide was enhanced on all the resistant gonococci after incubation with fresh human serum. Control susceptible gonococci were devoid of the polysaccharide after incubation with heated human serum. Identical results were obtained with a fresh gonococcal isolate which had lost serum resistance on subculture but which, in common with 3 other isolates, was restored to serum resistance by incubation with CMP-NANA.


FEMS Microbiol Lett


Fox AJ,Curry A,Rowland PL,Lancaster S,Jones DM,Parsons NJ,Cole JA,Smith H




Has Abstract


1990-01-01 00:00:00












  • Polymerase chain reaction for identification of human and porcine spirochaetes recovered from cases of intestinal spirochaetosis.

    abstract::A polymerase chain reaction (PCR) amplification of 16S rDNA was developed to identify spirochaetes recovered from cases of intestinal spirochaetosis in humans and pigs; these bacteria belong to a distinct genetic group of spirochaetes, with the proposed name 'Anguillina coli'. The PCR incorporated a universal eubacter...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Park NY,Chung CY,McLaren AJ,Atyeo RF,Hampson DJ

    更新日期:1995-01-15 00:00:00

  • Energy production and peptidase activity in Eikenella corrodens.

    abstract::Eikenella corrodens 33EK(L), a clinical isolate, was assayed for its ability to utilise amino acids as substrates in the reduction of nitrate to nitrite. The metabolism of proline, glutamate, serine and glutamine was found to result in relatively high rates of nitrate reduction. The ability of cells to metabolise thes...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gully NJ,Rogers AH

    更新日期:1996-06-01 00:00:00

  • Mini-Tn7 vectors as genetic tools for gene cloning at a single copy number in an industrially important and phytopathogenic bacteria, Xanthomonas spp.

    abstract::Transposon mini-Tn7 vectors insert into the chromosome of several Gram-negative bacteria in a site-specific manner. Here, we showed the application of mini-Tn7 as single copy site-specific integration vector system for Xanthomonas campestris pv. campestris. The transposition of the mini-Tn7 into the bacterial genome w...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jittawuttipoka T,Buranajitpakorn S,Fuangthong M,Schweizer HP,Vattanaviboon P,Mongkolsuk S

    更新日期:2009-09-01 00:00:00

  • The arbuscular mycorrhizal fungus, Glomus irregulare, controls the mycotoxin production of Fusarium sambucinum in the pathogenesis of potato.

    abstract::Trichothecenes are an important family of mycotoxins produced by several species of the genus Fusarium. These fungi cause serious disease on infected plants and postharvest storage of crops, and the toxins can cause health problems for humans and animals. Unfortunately, there are few methods for controlling mycotoxin ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ismail Y,McCormick S,Hijri M

    更新日期:2013-11-01 00:00:00

  • Involvement of oxidative stress in the regulation of H(2)S production during ultradian metabolic oscillation of Saccharomyces cerevisiae.

    abstract::Periodic evolution of H(2)S during aerobic chemostat culture of Saccharomyces cerevisiae resulted in ultradian metabolic oscillation via periodic inhibition of respiratory activity. To understand the nature of periodic H(2)S evolution, we investigated whether oxidative stress is associated with H(2)S production. The c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kwak WJ,Kwon GS,Jin I,Kuriyama H,Sohn HY

    更新日期:2003-02-14 00:00:00

  • Far different levels of gene expression provided by an oriented cloning system in Bacillus subtilis and Escherichia coli.

    abstract::A gene expression system for both Bacillus subtilis and Escherichia coli was developed. The expression vector, pHASH102, produces any combination of promoter and open reading frame to be expressed based on the T-extended cloning method. Because the pHASH series vectors are designed to shuttle between the genome and a ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ohashi Y,Ohshima H,Tsuge K,Itaya M

    更新日期:2003-04-11 00:00:00

  • DNA from Aspergillus flavus contains 5-methylcytosine.

    abstract::DNA from Aspergillus sp. has been reported not to contain 5-methylcytosine. However, it has been found that Aspergillus nidulans responds to 5-azacytidine, a drug that is a strong inhibitor of DNA methyltransferases. Therefore, we have re-examined the occurrence of 5-methylcytosine in DNA from Aspergillus flavus by us...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gowher H,Ehrlich KC,Jeltsch A

    更新日期:2001-11-27 00:00:00

  • Mutation in dipZ leads to reduced production of active human placental alkaline phosphatase in Escherichia coli.

    abstract::We have tested the expression of alkaline phosphatases in a mutant strain of Escherichia coli deficient in dipZ, a gene coding for a protein involved in cytochrome c biogenesis, and the isogenic wild-type strain. The yield of soluble and active human placental alkaline phosphatase was significantly reduced in the muta...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Beck R,Crooke H,Jarsch M,Cole J,Burtscher H

    更新日期:1994-12-01 00:00:00

  • Multiple restriction-modification systems are present in rumen treponemes.

    abstract::Type II restriction endonucleases were purified by heparin-sepharose followed by ion chromatography from Treponema strains. The results indicate that in addition to frequently cutting GATC-specific restriction enzymes, the tested strains also possess rarely cutting endonucleases. The purified restriction endonucleases...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Piknova M,Javorsky P,Pristas P

    更新日期:2005-10-01 00:00:00

  • Mycobacterium marinum biofilm formation reveals cording morphology.

    abstract::Abstract The emergence of the nontuberculosis mycobacteria (NTM) as clinically relevant pathogens has warranted the study of these ubiquitous organisms in the context of their likely environmental niche, the biofilm. We assayed the NTM bacterium Mycobacterium marinum strain 1218R, a fish outbreak isolate, for biofilm ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hall-Stoodley L,Brun OS,Polshyna G,Barker LP

    更新日期:2006-04-01 00:00:00

  • A PCR-based method to characterise and identify benzimidazole resistance in Helminthosporium solani.

    abstract::Control of Helminthosporium solani, the cause of silver scurf in potato tubers, has been impaired by selection of benzimidazole-resistant strains as a result of repeated use of the fungicide thiabendazole. Identification of thiabendazole-resistant strains of H. solani by conventional techniques takes several weeks. Pr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: McKay GJ,Cooke LR

    更新日期:1997-07-15 00:00:00

  • Genetic evidence that hepA gene is involved in the normal deposition of the envelope of both heterocysts and akinetes in Anabaena variabilis ATCC 29413.

    abstract::The hepA gene in Anabaena sp. strain PCC 7120 is required for normal formation of the polysaccharide layer of the heterocyst envelope. A plasmid bearing hepA, interrupted by a neomycin-resistance cassette, was transferred by conjugation to wild type Anabaena variabilis ATCC 29413, so that the interrupted hepA gene rep...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Leganés F

    更新日期:1994-10-15 00:00:00

  • A leader open reading frame is essential for the expression in Escherichia coli of GC-rich leuB gene of an extreme thermophile, Thermus thermophilus.

    abstract::To improve expression efficiency of the leuB gene of an extreme thermophile. Thermus thermophilus, in Escherichia coli, the gene was placed under a potent promoter, tac. However, the expression was hardly improved, despite increased transcription. The expression under tac promoter was significantly improved by introdu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ishida M,Oshima T

    更新日期:1996-01-01 00:00:00

  • Oxacillin-hydrolyzing beta-lactamase involved in resistance to imipenem in Acinetobacter baumannii.

    abstract::Acinetobacter baumannii strain A148, a clinical isolate resistant to imipenem (MIC = 32 mg l-1), synthesized two beta-lactamases with pIs 6.3 and > 9.2. The pI 6.3 enzyme hydrolyzed the penicillins, including isoxazoylpenicillins, first-, second- and, to a lesser extent, third-generation cephalosporins. It was inhibit...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hornstein M,Sautjeau-Rostoker C,Péduzzi J,Vessières A,Hong LT,Barthélémy M,Scavizzi M,Labia R

    更新日期:1997-08-15 00:00:00

  • Description of two new species of Aliinostoc and one new species of Desmonostoc from India based on the Polyphasic Approach and reclassification of Nostoc punensis to Desmonostoc punense comb. nov.

    abstract::Three heterocytous cyanobacterial strains were isolated from different habitats of Central India, and initial morphological studies indicated them to be members of the genus Nostoc or other closely related genera. Subsequent studies using morphological, ecological, molecular and phylogenetic methods indicated the thre...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Saraf A,Dawda HG,Suradkar A,Behere I,Kotulkar M,Shaikh ZM,Kumat A,Batule P,Mishra D,Singh P

    更新日期:2018-12-01 00:00:00

  • Pre-exposure to UV irradiation increases the transfer frequency of the IncJ conjugative transposon-like elements R391, R392, R705, R706, R997 and pMERPH and is recA+ dependent.

    abstract::The enteric conjugative transposon-like IncJ elements R391, R392, R705, R706 and pMERPH, all demonstrated increased conjugative transfer upon UV irradiation. The transfer frequency increased on average from its basal rate of 10(-5) to 10(-3) per recipient, upon pre-exposure to UV irradiation. However, the transfer fre...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: McGrath BM,O'Halloran JA,Pembroke JT

    更新日期:2005-02-15 00:00:00

  • Streptococcus uberis acquires plasmin activity following growth in the presence of bovine plasminogen through the action of its specific plasminogen activator.

    abstract::Three (0140J, C197C and EF20) out of four strains of Streptococcus uberis exhibited high levels of bound plasmin activity following growth in the presence of bovine plasminogen. The remaining strain (C197) bound considerably less plasmin following growth in the same medium. In contrast to the others, this strain was u...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Leigh JA,Lincoln RA

    更新日期:1997-09-01 00:00:00

  • Stereoselective biosynthesis of chloroarylpropane diols by the basidiomycete Bjerkandera adusta: exploring the roles of amino acids, pyruvate, glycerol and phenyl acetyl carbinol.

    abstract::Bjerkandera adusta produces many chlorometabolites including chlorinated anisyl metabolites (CAMs) and 1-arylpropane-1,2-diols (1, 2, 3, 4) as idiophasic metabolic products of L-phenylalanine. These diols are stereoselectively biosynthesized from a C7-unit (benzylic, from L-phenylalanine) and a C2-unit, of unknown ori...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Silk PJ,Macaulay JB

    更新日期:2003-11-07 00:00:00

  • Borrelia burgdorferi possesses a collagenolytic activity.

    abstract::Lyme disease is a multisystemic disorder caused by Borrelia burgdorferi, an invasive spirochete. B. burgdorferi has a predilection for collagenous tissue and one major clinical manifestation of the disease is arthritis. We have identified a collagenolytic activity in B. burgdorferi detergent lysates using iodinated ge...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Grab DJ,Kennedy R,Philipp MT

    更新日期:1996-10-15 00:00:00

  • Growth of Salmonella enteritidis and S. pullorum on Hektoen agar and the expression of lipopolysaccharide or flagella.

    abstract::Growth of strains of Salmonella enteritidis and Salmonella pullorum on Hektoen agar has been reported to influence the expression of long-chain lipopolysaccharide and motility respectively. In this study we used a panel of strains of S. enteritidis and S. pullorum to investigate these phenomena. Culture on Hektoen aga...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Chart H,Rowe B

    更新日期:1998-06-15 00:00:00

  • Importance of fumarate and nitrate reduction regulatory protein for intestinal proliferation of Vibrio vulnificus.

    abstract::The sepsis caused by Vibrio vulnificus is characterized by an average incubation period of 26 h and a high mortality rate exceeding 50%. The fast growth and dissemination of V. vulnificus in vivo lead to poor clinical outcomes in patients. Therefore, elucidation of the proliferation mechanisms of this organism in vivo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kado T,Kashimoto T,Yamazaki K,Ueno S

    更新日期:2017-01-01 00:00:00

  • Fatty acids synthesized by oral treponemes in chemically defined media.

    abstract::OMIZ-W68, a chemically defined medium that contains no long-chain fatty acids and yet supports in vitro proliferation of a wide range of fastidious oral anaerobes, is described. The type strains of Treponema denticola, Treponema lecithinolyticum, Treponema maltophilum, Treponema pectinovorum, Treponema socranskii, and...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wyss C

    更新日期:2007-04-01 00:00:00

  • Identification of mature appressorium-enriched transcripts in Magnaporthe grisea, the rice blast fungus, using suppression subtractive hybridization.

    abstract::We have constructed a fungal subtractive suppressive library enriched in genes expressed during appressorium maturation in Magnaporthe grisea. Sequencing of 250 clones from the subtracted appressorium cDNA library revealed 142 unique genes, represented by 155 non-redundant ESTs (expressed sequence tags). Of these ESTs...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lu JP,Liu TB,Lin FC

    更新日期:2005-04-01 00:00:00

  • Roseovarius sp. strain 217: aerobic taurine dissimilation via acetate kinase and acetate-CoA ligase.

    abstract::The genome sequence of Roseovarius sp. strain 217 indicated that many pathway enzymes found in other organisms for the degradation of taurine are represented, but that a novel, apparently energy-dependent pathway is involved in the conversion of acetyl phosphate to acetyl CoA. Thus, an ABC transporter for taurine coul...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Baldock MI,Denger K,Smits TH,Cook AM

    更新日期:2007-06-01 00:00:00

  • Conserved motifs in the CRISPR leader sequence control spacer acquisition levels in Type I-D CRISPR-Cas systems.

    abstract::Integrating short DNA fragments at the correct leader-repeat junction is key to successful CRISPR-Cas memory formation. The Cas1-2 proteins are responsible to carry out this process. However, the CRISPR adaptation process additionally requires a DNA element adjacent to the CRISPR array, called leader, to facilitate ef...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kieper SN,Almendros C,Brouns SJJ

    更新日期:2019-06-01 00:00:00

  • Properties of uracil transport by vegetative mycelium of Trichoderma viride.

    abstract::The transport of radioactively labelled uracil into submerged mycelium of T. viride was measured by means of a membrane filtration technique. It was found to be time-dependent (up to 90 min) and concentration-dependent (up to 8 mmol l-1). Its concentration dependence was biphasic and consisted from the saturatable par...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lakatos B,Simkovic M,Betina V,Varecka L

    更新日期:1999-02-15 00:00:00

  • Allelic exchange and site-directed mutagenesis probe the contribution of ActA amino-acid variability to phosphorylation and virulence-associated phenotypes among Listeria monocytogenes strains.

    abstract::To test the hypothesis that actA allelic variation contributes to virulence differences among Listeria monocytogenes strains, cell-to-cell spread and intracellular ActA phosphorylation patterns were characterized for 14 wild-type isolates and selected isogenic mutants. Our data show that (i) while actA allelic variati...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Roberts AJ,Wiedmann M

    更新日期:2006-01-01 00:00:00

  • Characterization of a novel vip3-type gene from Bacillus thuringiensis and evidence of its presence on a large plasmid.

    abstract::A novel vip3-type gene named vip3LB has been isolated from Bacillus thuringiensis strain BUPM95. The corresponding secreted vegetative insecticidal protein was active against the lepidopteran insect Ephestia kuehniella. The vip3LB gene was shown, for the first time, to be carried by the large plasmid containing the cr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mesrati LA,Tounsi S,Jaoua S

    更新日期:2005-03-15 00:00:00

  • Expression and activity of the Calvin-Benson-Bassham cycle transcriptional regulator CbbR from Acidithiobacillus ferrooxidans in Ralstonia eutropha.

    abstract::Autotrophic fixation of carbon dioxide into cellular carbon occurs via several pathways but quantitatively, the Calvin-Benson-Bassham cycle is the most important. CbbR regulates the expression of the cbb genes involved in CO2 fixation via the Calvin-Benson-Bassham cycle in a number of autotrophic bacteria. A gene pote...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Esparza M,Jedlicki E,Dopson M,Holmes DS

    更新日期:2015-08-01 00:00:00

  • Cloning of xylanase gene of Streptomyces flavogriseus in Escherichia coli and bacteriophage lambda-induced lysis for the release of cloned enzyme.

    abstract::The xylanase gene of Streptomyces flavogriseus was cloned in pUC8 plasmid and expressed in Escherichia coli lysogenic for lambda cI857. lambda-Induced lysis of E. coli at 42 degrees C allowed efficient release of cloned enzyme activity in extracellular environment. The xylanase gene was located in the 0.8-kb HindIII f...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Srivastava R,Ali SS,Srivastava BS

    更新日期:1991-03-01 00:00:00