Abstract:
:Proteus mirabilis strain MAG1, a clinical isolate that is resistant to broad-spectrum penicillins and co-amoxiclav, produces inhibitor-resistant TEM (IRT)-21, a novel mutant of TEM beta-lactamase. This enzyme has a pI of 5.2 and is derived from the bla(TEM-1a) gene ancestor. It contains two major amino acid substitutions specific for co-amoxiclav resistance (Leu-69 for Met and Ser-244 for Arg) that have never been found together previously. The dramatic loss of sensitivity to clavulanic acid, the enhancement of K(m) for all beta-lactams and markedly for ticarcillin, and the decrease in the catalytic efficiency makes IRT-21 comparable to the other IRTs with substitutions at position 244 or double substitutions.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Mammeri H,Gilly L,Laurans G,Vedel G,Eb F,Paul Gdoi
10.1111/j.1574-6968.2001.tb10945.xkeywords:
subject
Has Abstractpub_date
2001-12-18 00:00:00pages
185-9issue
2eissn
0378-1097issn
1574-6968pii
S037810970100461Xjournal_volume
205pub_type
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