Abstract:
:Bacteria carry a number of genes that cause cell growth arrest or cell lysis upon expression. Notably, defective prophages retain many lysis proteins. Here, we identified a novel lytic gene, ydfD, on the Qin prophage segment of the Escherichia coli genome. YdfD lyses 99.9% of cells within 2 h of its induction. The co-expression of the upstream gene, dicB, encoding a cell division inhibitor, as well as sulA, encoding another cell division inhibitor, abolished YdfD-induced cell lysis. These results imply that YdfD-induced lysis is a cell division-dependent event. We further found that by deleting the hydrophobic 22-residue N-terminal domain, the resulting 42-residue C-terminal domain was still toxic to cause cell lysis. We propose that YdfD, associated with the cytoplasmic membrane, inhibits an essential cellular process(s).
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Masuda H,Awano N,Inouye Mdoi
10.1093/femsle/fnw039subject
Has Abstractpub_date
2016-03-01 00:00:00issue
6eissn
0378-1097issn
1574-6968pii
fnw039journal_volume
363pub_type
杂志文章abstract::We have isolated the abc1 gene from the fission yeast Schizosaccharomyces pombe. Sequence analysis suggests that the Abc1 protein is a member of the ABC superfamily of transporters and is composed of two structurally homologous halves, each consisting of a hydrophobic region of six transmembrane domains and a hydrophi...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:1991-03-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 信件
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:2010-01-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:1999-02-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:2001-08-07 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章,评审
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更新日期:2000-12-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:2000-08-15 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:1997-10-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:1993-12-15 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2002.tb11126.x
更新日期:2002-04-09 00:00:00