ydfD encodes a novel lytic protein in Escherichia coli.

Abstract:

:Bacteria carry a number of genes that cause cell growth arrest or cell lysis upon expression. Notably, defective prophages retain many lysis proteins. Here, we identified a novel lytic gene, ydfD, on the Qin prophage segment of the Escherichia coli genome. YdfD lyses 99.9% of cells within 2 h of its induction. The co-expression of the upstream gene, dicB, encoding a cell division inhibitor, as well as sulA, encoding another cell division inhibitor, abolished YdfD-induced cell lysis. These results imply that YdfD-induced lysis is a cell division-dependent event. We further found that by deleting the hydrophobic 22-residue N-terminal domain, the resulting 42-residue C-terminal domain was still toxic to cause cell lysis. We propose that YdfD, associated with the cytoplasmic membrane, inhibits an essential cellular process(s).

journal_name

FEMS Microbiol Lett

authors

Masuda H,Awano N,Inouye M

doi

10.1093/femsle/fnw039

subject

Has Abstract

pub_date

2016-03-01 00:00:00

issue

6

eissn

0378-1097

issn

1574-6968

pii

fnw039

journal_volume

363

pub_type

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