DNA probes for identification of the ectomycorrhizal fungus Tuber magnatum Pico.

Abstract:

:The random amplified polymorphic DNA (RAPD) technique was used to develop DNA probes for the identification of ectomycorrhizal fungi belonging to the genus Tuber. RAPD fingerprinting revealed a high degree of interspecific variability and a low degree of intraspecific variability. One band (approximately 1.5 kb), consistently appearing when genomic DNA was amplified with an aspecific primer (OPA-18), was found to be a good marker for Tuber magnatum, and was used as a probe in Southern hybridization experiments. The specificity of the results suggests that this probe may be useful in developing specific primers for PCR amplifications.

journal_name

FEMS Microbiol Lett

authors

Lanfranco L,Wyss P,Marzachí C,Bonfante P

doi

10.1111/j.1574-6968.1993.tb06581.x

subject

Has Abstract

pub_date

1993-12-15 00:00:00

pages

245-51

issue

3

eissn

0378-1097

issn

1574-6968

journal_volume

114

pub_type

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