The 70 kilodalton iron regulated protein of Neisseria meningitidis is not the human transferrin receptor.

Abstract:

:Neisseria meningitidis is able to chelate iron from human transferrin (HTF), the main sequestrator of extracellular iron in vivo. Previous workers have reported that a ca. 70 kilodalton (kDa) iron regulated outer membrane protein (FeRP-70) is a highly specific receptor for HTF. We have examined the interaction between the iron regulated outer membrane proteins (OMP's) and HTF, using HTF and rabbit anti HTF, as well as gold labelled HTF (Au-HTF) to blot OMP's of various serogroups and serotypes of N. meningitidis. Also, we used monospecific rabbit anti FeRP-70 in competitive experiments to determine the role of FeRP-70 in HTF-binding. Single proteins (molecular weights range ca. 60 to ca. 90 kDa) were identified in the OMP's from each strain which reacted with HTF. HTF failed to block the reaction between FeRP-70 and the OMP's, conversely anti FeRP-70 failed to block the HTF-binding reaction. We believe that the 70 kDa iron regulated protein of N. meningitidis is not a human transferrin receptor.

journal_name

FEMS Microbiol Lett

authors

Ala'Aldeen DA,Davies HA,Wall RA,Borriello SP

doi

10.1016/0378-1097(90)90409-j

subject

Has Abstract

pub_date

1990-05-01 00:00:00

pages

37-42

issue

1-2

eissn

0378-1097

issn

1574-6968

journal_volume

57

pub_type

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