Non-radioactive restriction fragment length polymorphism (RFLP) typing of Clostridium difficile.

abstract：:Mesophilic Aeromonas hydrophila from serotypes O:11 and O:34 grown in a glucose-rich medium produce a capsule that can be seen under light and electron microscopy. The purified capsular polysaccharide has a composition qualitatively similar for strains O:11 and O:34, but quantitatively different. The capsular polysacc...

journal_title：FEMS microbiology letters

authors： Martínez MJ,Simon-Pujol D,Congregado F,Merino S,Rubires X,Tomás JM

更新日期：1995-04-15 00:00:00

abstract：:Oidium sp. has been recovered as an endophyte in Terminalia catappa (tropical chestnut) in Costa Rica. The volatile organic compounds (VOCs) of this organism uniquely and primarily consist of esters of propanoic acid, 2-methyl-, butanoic acid, 2-methyl-, and butanoic acid, 3-methyl-. The VOCs of Oidium sp. are slightl...

journal_title：FEMS microbiology letters

authors： Strobel GA,Spang S,Kluck K,Hess WM,Sears J,Livinghouse T

更新日期：2008-06-01 00:00:00

abstract：:A search of the complete genome sequence of the halophilic archaeon Halobacterium salinarum failed to identify a gene homologous to the thymidylate synthase (thyA) gene present in the closely related Haloferax volcanii. To understand the source of thymidine synthesis in Hbt. salinarum, a genomic library of Hbt. salina...

journal_title：FEMS microbiology letters

authors： Giladi M,Bitan-Banin G,Mevarech M,Ortenberg R

更新日期：2002-10-29 00:00:00

abstract：:A previous study [DeVault et al. (1991) Mol. Microbiol. 5, 2503-2509] suggested that growth of Pseudomonas aeruginosa in glucose-containing medium represses algD gene transcription. In this study, growth of P. aeruginosa in rich medium containing glucose or gluconate increased alginate production and algD transcriptio...

journal_title：FEMS microbiology letters

authors： Ma JF,Phibbs PV,Hassett DJ

更新日期：1997-03-15 00:00:00

abstract：:Based on second generation sequencing (MiSeq platform, Illumina), we determined the genetic diversity of T4-like bacteriophages of the family Myoviridae by analysing fragments of the major capsid protein gene g23 in the plankton of Lake Baikal. The sampling depth in our study was significantly higher than in those obt...

journal_title：FEMS microbiology letters

authors： Potapov S,Belykh O,Krasnopeev A,Gladkikh A,Kabilov M,Tupikin A,Butina T

更新日期：2018-02-01 00:00:00

abstract：:Falcipain-2A, the cysteine protease of Plasmodium falciparum has been proposed as a good drug target. This study evaluated the suitability of Plasmodium berghei as the animal model and reports the first functional expression and characterization of the falcipain-2A orthologue, berghepain-2. Comparative studies reveale...

journal_title：FEMS microbiology letters

authors： Chan C,Goh LL,Sim TS

更新日期：2005-08-15 00:00:00

abstract：:The role of the flagellum and chemotactic motility of Vibrio anguillarum for phagocytosis by and intracellular survival in fish macrophages was determined using a wild-type strain, a mutant without the flagellum, a mutant with a truncated flagellum and a non-chemotactic mutant. For all strains, the numbers of intracel...

journal_title：FEMS microbiology letters

authors： Larsen MH,Boesen HT

更新日期：2001-09-25 00:00:00

abstract：:The prodrug activation system formed by the E. coli codA gene encoding cytosine deaminase (CD) and 5-fluorocytosine (5-FC) developed for selective cancer chemotherapy suffers from a sensitivity limitation in many tumour cells. In an attempt to improve the CD/5-FC suicide association, we combined the E. coli upp gene e...

journal_title：FEMS microbiology letters

authors： Tiraby M,Cazaux C,Baron M,Drocourt D,Reynes JP,Tiraby G

更新日期：1998-10-01 00:00:00

abstract：:The pseudotrisaccharide allosamidin, a potent inhibitor of chitinases, retarded the fragmentation of hyphae but did not affect the fungal growth and cephalosporin-C production in Acremonium chrysogenum. In vitro inhibition of A. chrysogenum cell-bound chitinase(s) by allosamidin revealed that about 47% of the soluble ...

journal_title：FEMS microbiology letters

authors： Sándor E,Pusztahelyi T,Karaffa L,Karányi Z,Pócsi I,Biró S,Szentirmai A,Pócsi I

更新日期：1998-07-15 00:00:00

abstract：:The first prokaryotic urea carboxylase has previously been purified and characterized from Oleomonas sagaranensis. As the results indicated the presence of an ATP-dependent urea degradation pathway in Bacteria, the characterization of the second component of this pathway, allophanate hydrolase, was carried out. The ge...

journal_title：FEMS microbiology letters

authors： Kanamori T,Kanou N,Kusakabe S,Atomi H,Imanaka T

更新日期：2005-04-01 00:00:00

abstract：:The application of series of high intensity electric pulses to a yeast suspension provoked a considerable release of some cytoplasmic proteins, glutathione reductase, 3-phosphoglycerate kinase and alcohol dehydrogenase. A maximal yield was achieved 3-8 h after pulsation. The electro-induced protein efflux was accelera...

journal_title：FEMS microbiology letters

authors： Ganeva V,Galutzov B

更新日期：1999-05-15 00:00:00

abstract：:Two actinomycete strains, CHR3 and CHR28, were isolated from metal-contaminated sediments from Baltimore Inner Harbor. The isolates were classified as Streptomyces spp. Agar diffusion assays showed both isolates to be resistant to mercuric chloride and phenylmercuric acetate. Hybridization experiments indicated that g...

journal_title：FEMS microbiology letters

authors： Ravel J,Amoroso MJ,Colwell RR,Hill RT

更新日期：1998-05-01 00:00:00

abstract：:Phylogenetically diverse rhizobial strains endemic to Tunisia were isolated from symbiotic nodules of Lotus creticus, growing in different arid extremophile geographical regions of Tunisia, and speciated using multiloci-phylogenetic analysis as Neorhizobium huautlense (LCK33, LCK35, LCO42 and LCO49), Ensifer numidicus...

journal_title：FEMS microbiology letters

authors： Rejili M,BenAbderrahim MA,Mars M,Sherrier JD

更新日期：2020-06-01 00:00:00

abstract：:This study provides a novel qRT-PCR protocol for specific detection and proof of viability of Phytophthora in environmental samples based on differential accumulation of cox II transcripts. Chemical and physical treatments were tested for their ability to induce in vitro the accumulation of cytochrome oxidase genes en...

journal_title：FEMS microbiology letters

authors： Vannini A,Tomassini A,Bruni N,Vettraino AM

更新日期：2014-04-01 00:00:00

abstract：:Twelve strains of Candida albicans were grown in defined medium which had been deferrated by ion-exchange chromatography and then supplemented with FeCl3 to give iron concentrations ranging from 0.026 microM (growth-limiting) to 0.8 microM (excess). All of the strains secreted hydroxamate-type siderophores; phenolate ...

journal_title：FEMS microbiology letters

authors： Sweet SP,Douglas LJ

更新日期：1991-05-01 00:00:00

abstract：:Volvariella volvacea, strain V14, produces multiple forms of extracellular laccase when grown in submerged culture in a defined medium with glucose as sole carbon source, and on cotton waste 'compost' representative of the conditions used for industrial-scale mushroom cultivation. In liquid culture, enzyme synthesis i...

journal_title：FEMS microbiology letters

authors： Chen S,Ma D,Ge W,Buswell JA

更新日期：2003-01-21 00:00:00

abstract：:We found two Shiga toxin producing Escherichia coli O157:H7 strains isolated from humans carrying the stx(1) gene with an IS1203-like element (designated as IS1203v(1)). The IS1203v(1) was inserted into the coding region of the A subunit 7 bp upstream from the TGA termination codon, resulting in a loss of two amino ac...

journal_title：FEMS microbiology letters

authors： Suzuki M,Kondo F,Ito Y,Matsumoto M,Hata M,Oka H,Takahashi M,Sakae K

更新日期：2004-05-01 00:00:00

abstract：:Growth of a Mycobacterium avium complex serotype 20 rough-colony variant in 1.0% Tween 80 resulted in a smooth-colony morphological conversion that was reversible upon removal of Tween and was not associated with the presence of serotype-specific glycopeptidolipid antigens. Electron microscopic examination suggested t...

journal_title：FEMS microbiology letters

authors： Wright EL,Pourshafie M,Barrow WW

更新日期：1992-11-01 00:00:00

abstract：:Fourteen strains representing 11 Caragana-nodulating Mesorhizobium genomic species were identified as representing Mesorhizobium amorphae, Mesorhizobium huakuii, Mesorhizobium septentrionale and groups related to Mesorhizobium plurifarium, Mesorhizobium temperatum, Mesorhizobium tianshanense and Mesorhizobium mediterr...

journal_title：FEMS microbiology letters

authors： Chen WF,Guan SH,Zhao CT,Yan XR,Man CX,Wang ET,Chen WX

更新日期：2008-06-01 00:00:00

abstract：:Changes in composition of the principal low molecular mass thiols of Leishmania donovani were monitored during the transformation of promastigotes, first to stationary phase metacyclic forms and then to amastigotes. No consistent variation in the thiol composition of the parasite which could account for the known incr...

journal_title：FEMS microbiology letters

authors： Weldrick DP,Chodacka B,Vogt R,Steenkamp DJ

更新日期：1999-04-01 00:00:00

abstract：:The 0104 antigen (lipopolysaccharide, LPS) of Escherichia coli has an acidic O specific polysaccharide. From the aqueous phase of a phenol water extraction of E. coli O104: K-, a fraction was obtained by ultracentrifugation and Cetavlon precipitation of the supernatant, which was enriched in long-chain LPS. Compositio...

journal_title：FEMS microbiology letters

authors： Kogan G,Jann B,Jann K

更新日期：1992-03-01 00:00:00

abstract：:Sixty-four breast-fed infants and 23 calves were investigated for bacteria and enzymatic activity in their faecal samples. The bacteria were measured using cultivation and fluorescence in situ hybridization. Enzymatic activity was also examined. Forty-seven (64%) infants and all the calves had high numbers of bifidoba...

journal_title：FEMS microbiology letters

authors： Rada V,Vlková E,Nevoral J,Trojanová I

更新日期：2006-05-01 00:00:00

abstract：:Clostridium thermocellum, a Gram-positive, thermophilic anaerobe produces a highly active cellulase system. This system, termed the cellulosome, is a complex composed of at least 14-18 different types of components organized around a large, cellulose-binding protein. Combining recombinant DNA technology and protein bi...

journal_title：FEMS microbiology letters

authors： Béguin P,Millet J,Aubert JP

更新日期：1992-12-15 00:00:00

abstract：:The aim of this study was the production of the homopolyester poly(4-hydroxybutyric acid) (poly(4HB)) with recombinant strains of Escherichia coli. Wild-type strains and other widely used non-recombinant strains of E. coli are not able to produce polyhydroxyalkanoic acids (PHA) as storage compounds and cannot utilize ...

journal_title：FEMS microbiology letters

authors： Hein S,Söhling B,Gottschalk G,Steinbüchel A

更新日期：1997-08-15 00:00:00

abstract：:In this study, we evaluated if recA species-specific PCR assays could be successfully applied to identify environmental isolates of the widespread Burkholderia cepacia complex (Bcc) species. A total of 729 Bcc rhizosphere isolates collected in different samplings were assigned to the species B. cepacia genomovar I (61...

journal_title：FEMS microbiology letters

authors： Dalmastri C,Pirone L,Tabacchioni S,Bevivino A,Chiarini L

更新日期：2005-05-01 00:00:00

abstract：:Bacillus sp. strain CS93, which was previously isolated from Pozol, was previously shown to produce iturin A, bacilysin and chlorotetaine. To investigate the biosynthetic mechanism of chlorotetaine production, the bac genes were amplified from genomic DNA of Bacillus sp. CS93 by PCR and sequenced. The genes bacABCDE w...

journal_title：FEMS microbiology letters

authors： Moran S,Robertson K,Paradisi F,Rai DK,Murphy CD

更新日期：2010-03-01 00:00:00

abstract：:The alternative sigma factor sigmaH has been shown to play an important role in stress response and morphological differentiation in Streptomyces coelicolor. Its gene, sigH, is located in an operon with the gene encoding proposed anti-sigma factor UshX, and one of the promoters directing expression of the operon is de...

journal_title：FEMS microbiology letters

authors： Sevcikova B,Kormanec J

更新日期：2002-04-09 00:00:00

abstract：:To explore the discriminatory power of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for detecting subtle differences in isogenic isolates, we tested isogenic strains of Staphylococcus aureus differing in their expression of resistance to methicillin or teicoplanin. More i...

journal_title：FEMS microbiology letters

authors： Majcherczyk PA,McKenna T,Moreillon P,Vaudaux P

更新日期：2006-02-01 00:00:00

abstract：:It is now well established that physicochemical properties of exopolysaccharides (EPS) can vary between strains of a given species and according to growth conditions. The EPS production of four strains of Lactobacillus bulgaricus was monitored during growth in milk and in a chemically defined media. All strains, inclu...

journal_title：FEMS microbiology letters

authors： Petry S,Furlan S,Waghorne E,Saulnier L,Cerning dagger J,Maguin E

更新日期：2003-04-25 00:00:00

abstract：:Cyanobacterial natural products offer new possibilities for drugs and lead compounds but many factors can inhibit the production of sufficient yields for pharmaceutical processes. While Escherichia coli and Streptomyces sp. have been used as heterologous expression hosts to produce cyanobacterial natural products, the...

journal_title：FEMS microbiology letters

authors： Wells KN,Videau P,Nelson D,Eiting JE,Philmus B

更新日期：2018-08-01 00:00:00