Differences in biochemical properties of the Plasmodial falcipain-2 and berghepain-2 orthologues: implications for in vivo screens of inhibitors.

abstract：:The synthesis of short chain S-methyl thioesters was investigated in Geotrichum candidum strain GcG. The results indicated the involvement of an enzymatic reaction in this microorganism that led to the synthesis of S-methyl thioacetate (MTA) when methanethiol and acetyl-CoA were used as substrates. MTA was generated f...

journal_title：FEMS microbiology letters

authors： Helinck S,Spinnler HE,Parayre S,Dame-Cahagne M,Bonnarme P

更新日期：2000-12-15 00:00:00

abstract：:Hemerythrins are oxygen-binding proteins found in the body fluids and tissues of certain invertebrates. Oxygen is bound at a nonheme iron centre consisting of two oxo-bridged iron atoms bound to a characteristic set of conserved histidine: aspartate and glutamate residues with the motifs H-HxxxE-HxxxH-HxxxxD. It has r...

journal_title：FEMS microbiology letters

authors： French CE,Bell JM,Ward FB

更新日期：2008-02-01 00:00:00

abstract：:The marine dinoflagellate genus Alexandrium includes a number of species that produce potent neurotoxins responsible for paralytic shellfish poisoning, which in humans may cause muscular paralysis, neurological symptoms and, in extreme cases, death. Because of the genetic diversity of different genera and species, mol...

journal_title：FEMS microbiology letters

authors： Wang L,Li L,Alam MJ,Geng Y,Li Z,Yamasaki S,Shi L

更新日期：2008-05-01 00:00:00

abstract：:We report the draft genome sequence from Aeromonas salmonicida sp. strain CBA100, which was characterized as an antibiotic-resistant bacterium isolated from infected rainbow trout. The total size of the genome is 4,788,109 bp, with a G + C content of 60.55%. Comparison of its open reading frames shows that the closest...

journal_title：FEMS microbiology letters

authors： Valdes N,Espinoza C,Sanhueza L,Gonzalez A,Corsini G,Tello M

更新日期：2015-03-01 00:00:00

abstract：:The ecology of aerobic microorganisms is never explored in marine oxygen minimum zone (OMZ) sediments. Here we reveal aerobic bacterial communities along ∼3 m sediment-horizons of the eastern Arabian Sea OMZ. Sulfide-containing sediment-cores retrieved from 530 mbsl (meters beneath the sea-level) and 580 mbsl were exp...

journal_title：FEMS microbiology letters

authors： Bhattacharya S,Roy C,Mandal S,Sarkar J,Rameez MJ,Mondal N,Mapder T,Chatterjee S,Pyne P,Alam M,Haldar PK,Roy R,Fernandes S,Peketi A,Chakraborty R,Mazumdar A,Ghosh W

更新日期：2020-10-16 00:00:00

abstract：:ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite has potential biomedical and food applications due to its better antimicrobial activity. However, the presence of nano-metal-oxide in the composite makes the material unsuitable for any food applications. Moreover, the cost involved in the preparation of Zinc Oxide-Nano-...

journal_title：FEMS microbiology letters

authors： Visnuvinayagam S,Murthy LN,Parvathy U,Jeyakumari A,Sivaraman GK,Karthikeyan D

更新日期：2020-12-15 00:00:00

abstract：:Chagas disease, caused by Trypanosoma cruzi, affects six to seven million people worldwide. Treatment is based on benznidazole, producing several side effects and debatable efficacy, highlighting the need for new alternative drugs. We investigated the activity of four C-4 functionalized azlactone derivatives (EPA-27, ...

journal_title：FEMS microbiology letters

authors： de Azeredo CM,Ávila EP,Pinheiro DL,Amarante GW,Soares MJ

更新日期：2017-02-01 00:00:00

abstract：:The 16S ribosomal RNA gene sequences of Lactococcus lactis ssp. lactis and ssp. cremoris differ by 9-10 bp (depending on strain), within the first 200 bp of the sequence. These differences were used to develop two methods of genetically differentiating lactis and cremoris strains. Primers to conserved sequences in the...

journal_title：FEMS microbiology letters

authors： Ward LJ,Brown JC,Davey GP

更新日期：1998-09-01 00:00:00

abstract：:Phospholipid etherlipid (PLEL) derived isoprenoids and phospholipid fatty acids (PLFA) were determined in eight Euryarchaeotal monocultures for taxonomic profiling. For the first time significant amounts of fatty acids in the PLFA of Euryarchaeota were determined. The PLFA proportion varied between 11.3 and 35.5% of t...

journal_title：FEMS microbiology letters

authors： Gattinger A,Schloter M,Munch JC

更新日期：2002-07-16 00:00:00

abstract：:Mycobacterium avium ssp. paratuberculosis (M. paratuberculosis), the causative agent of Johne's disease, is an important animal pathogen that has also been implicated in human disease. The major proteins expressed by M. paratuberculosis were analyzed by two-dimensional gel electrophoresis, and a superoxide dismutase (...

journal_title：FEMS microbiology letters

authors： Liu X,Feng Z,Harris NB,Cirillo JD,Bercovier H,Barletta RG

更新日期：2001-08-21 00:00:00

abstract：:To increase knowledge on haem biosynthesis in filamentous fungi like Aspergillus niger, pathway-specific gene expression in response to haem and haem intermediates was analysed. This analysis showed that iron, 5'-aminolevulinic acid (ALA) and possibly haem control haem biosynthesis mostly via modulating expression of ...

journal_title：FEMS microbiology letters

authors： Franken AC,Lokman BC,Ram AF,van den Hondel CA,de Weert S,Punt PJ

更新日期：2012-10-01 00:00:00

abstract：:The effects of oxidoreduction potential (Eh) combined with acetic acid, NaCl and temperature on the growth, acidification, and membrane properties of Lactobacillus plantarum were studied. The culture medium was set at pH 5, and two different Eh values were adjusted using nitrogen (Eh = +350 mV) or hydrogen (Eh = -300 ...

journal_title：FEMS microbiology letters

authors： Ouvry A,Waché Y,Tourdot-Maréchal R,Diviès C,Cachon R

更新日期：2002-09-10 00:00:00

abstract：:Members of the genus Actinoplanes are considered to be representative of motile actinomycetes. To infer the flagellar diversity of Actinoplanes species, novel degenerate primers were designed for the flagellin (fliC) gene. The fliC gene of 21 Actinoplanes strains was successfully amplified and classified into two grou...

journal_title：FEMS microbiology letters

authors： Yamamura H,Hanawa K,Kusunoki M,Nakamura K,Nakagawa Y,Ishida Y,Hamada M,Otoguro M,Tamura T,Fujita N,Hayakawa M

更新日期：2011-11-01 00:00:00

abstract：:An iterative position-specific score matrix (PSSM)-based approach was used to predict sigma(28) promoters in 11 Shewanella genomes. The Shewanella Correlation Browser was used to distinguish true-positive predictions from false-positive predictions in Shewanella oneidensis MR-1 by generating a sigma(28)-regulated tran...

journal_title：FEMS microbiology letters

authors： Song W,Juhn FS,Naiman DQ,Konstantinidis KT,Gardner TS,Ward MJ

更新日期：2008-06-01 00:00:00

abstract：:Biofilms present complex assemblies of micro-organisms attached to surfaces. they are dynamic structures in which various metabolic activities and interactions between the component cells occur. When phage come in contact with biofilms, further interactions occur dependent on the susceptibility of the biofilm bacteria...

journal_title：FEMS microbiology letters

authors： Sutherland IW,Hughes KA,Skillman LC,Tait K

更新日期：2004-03-12 00:00:00

abstract：:The riboflavin kinase encoding gene ribR is situated within a 12 genes locus ytmI-ytnM of the Bacillus subtilis chromosome. Here we demonstrate that ribR is transcribed as part of a 10 kb ytmI-ytnM operon. The riboflavin overproduction phenotype of B. subtilis ribC mutant strains, which is a result of the strongly red...

journal_title：FEMS microbiology letters

authors： Solovieva IM,Kreneva RA,Errais Lopes L,Perumov DA

更新日期：2005-02-01 00:00:00

abstract：:Viable counts and activities of sulfate-reducing bacteria were determined in the oral cavities of 12 healthy volunteers. Of these, 10 harboured viable sulfate-reducing bacteria populations. Six separate sites were sampled: the posterior tongue, anterior tongue, mid buccal mucosa, vestibular mucosa, supragingival plaqu...

journal_title：FEMS microbiology letters

authors： Willis CL,Gibson GR,Allison C,Macfarlane S,Holt JS

更新日期：1995-06-15 00:00:00

abstract：:Brief exposure of Pseudomonas aeruginosa to a temperature of 10 degrees C or lower caused a significant leakage of the periplasmic beta-lactamase into the medium. The extent of leakage increased as the incubation temperature was lowered to 4 degrees C and reached a maximum at 0 degrees C. Cells grown in the presence o...

journal_title：FEMS microbiology letters

authors： Lei Y,Satake S,Ishii J,Nakae T

更新日期：1991-05-15 00:00:00

abstract：:We have constructed a Thermus promoter probe vector pPP11 containing the promoterless heat-stable kanamycin resistance (Kmr) gene. Total DNA of T. thermophilus HB27 was randomly sheared into 100-200 bp fragments and inserted into the EcoRI site of pPP11. About 1000 clones which showed Kmr were obtained, and they were ...

journal_title：FEMS microbiology letters

authors： Maseda H,Hoshino T

更新日期：1995-05-01 00:00:00

abstract：:The enteric conjugative transposon-like IncJ elements R391, R392, R705, R706 and pMERPH, all demonstrated increased conjugative transfer upon UV irradiation. The transfer frequency increased on average from its basal rate of 10(-5) to 10(-3) per recipient, upon pre-exposure to UV irradiation. However, the transfer fre...

journal_title：FEMS microbiology letters

authors： McGrath BM,O'Halloran JA,Pembroke JT

更新日期：2005-02-15 00:00:00

abstract：:The response regulator DegU and its cognate histidine kinase DegS constitute a two-component system in the Gram-positive soil bacterium Bacillus subtilis. The phosphorylated form of DegU is known to activate transcription of more than 120 genes in B. subtilis, including the bpr gene encoding bacillopeptidase F. To cha...

journal_title：FEMS microbiology letters

authors： Tsukahara K,Ogura M

更新日期：2008-03-01 00:00:00

abstract：:FNR is an iron-binding transcriptional regulator of Escherichia coli which controls the expression of target genes in response to anaerobiosis. The mechanism used by FNR to sense and respond to anaerobiosis is unknown but it is thought to involve iron. In vitro transcription analyses have shown that iron-deficient FNR...

journal_title：FEMS microbiology letters

authors： Green J,Guest JR

更新日期：1993-10-15 00:00:00

abstract：:Two different nucleic acid precursor utilization patterns were obtained for five avian isolates of Chlamydia psittaci. Three of the isolates behaved in a manner similar to that previously described, showing total dependency on the host cell for ribonucleoside triphosphates and being unable to utilize medium-supplied t...

journal_title：FEMS microbiology letters

authors： McClarty G,Fan H,Andersen AA

更新日期：1993-04-15 00:00:00

abstract：:A DNA segment located immediately upstream of the eaeA gene of enterohemorrhagic Escherichia coli O157:H7 strain HA1 was cloned and sequenced. This segment contained an open reading frame encoding a predicted protein of 156 amino acids. A database search identified similar open reading frames upstream of the eaeA gene...

journal_title：FEMS microbiology letters

authors： Zhao S,Mitchell SE,Meng J,Doyle MP,Kresovich S

更新日期：1995-11-01 00:00:00

abstract：:The commercialization of the n-butanol bioprocess is largely dependent on the price of feedstocks. Renewable cellulose appears to be an appealing feedstock. The microbial production of n-butanol still remains challenging because of the limited availability of intracellular NADH. To address this issue, an Escherichia c...

journal_title：FEMS microbiology letters

authors： Saini M,Chiang CJ,Li SY,Chao YP

更新日期：2016-02-01 00:00:00

abstract：:Brucella usually carry two highly homologous genes (omp2a and omp2b) for porin-like proteins. In several B. abortus biovars the omp2a gene has a large deletion compared to other Brucella omp2's. In this study we have measured Omp2 pore activity in planar bilayers. Omp2b exhibits well-defined trimeric channel activity ...

journal_title：FEMS microbiology letters

authors： Mobasheri H,Ficht TA,Marquis H,Lea EJ,Lakey JH

更新日期：1997-10-01 00:00:00

abstract：:Staphylococcus aureus is responsible for a broad variety of chronic infections. Most S. aureus clinical isolates show the capacity to adhere to abiotic surfaces and to develop biofilms. Because S. aureus growing in a biofilm is highly refractory to treatment, inhibition of biofilm formation represents a major therapeu...

journal_title：FEMS microbiology letters

authors： Stenz L,François P,Fischer A,Huyghe A,Tangomo M,Hernandez D,Cassat J,Linder P,Schrenzel J

更新日期：2008-10-01 00:00:00

abstract：:The constitutive expression vector pHY300-F1gfp was constructed to test the function of a promoter, F1, cloned from the rice epiphyte Bacillus brevis strain DX01. The DX01 cells harboring the plasmid pHY300-F1gfp were shown to produce bright green fluorescence. The results were confirmed by Western blot analysis and f...

journal_title：FEMS microbiology letters

authors： Chen Y,Yan J,Yang M,Wang J,Shen D

更新日期：2003-12-05 00:00:00

abstract：:The phylogenetic interrelationships of the genera Pediococcus and Aerococcus were investigated using reverse transcriptase sequencing of 16S rRNA. The genus Pediococcus was found to be phylogenetically heterogeneous. The four species P. acidilactici, P. damnosus, P. parvulus and P. pentosaceus formed a phylogeneticall...

journal_title：FEMS microbiology letters

authors： Collins MD,Williams AM,Wallbanks S

更新日期：1990-08-01 00:00:00

abstract：:We report the enhanced bactericidal activity of ofloxacin in drug-containing Eudragit E100(®) dispersions (EuCl-OFX) against Pseudomonas aeruginosa and the effect of the cationic polymer on bacterial membrane. Organisms treated with EuCl-OFX showed changes in cell morphology, altered outer membrane (OM) and cytoplasm...

journal_title：FEMS microbiology letters

authors： Romero VL,Pons P,Bocco JL,Manzo RH,Alovero FL

更新日期：2012-09-01 00:00:00