Identification of a secreted superoxide dismutase in Mycobacterium avium ssp. paratuberculosis.

Abstract:

:Mycobacterium avium ssp. paratuberculosis (M. paratuberculosis), the causative agent of Johne's disease, is an important animal pathogen that has also been implicated in human disease. The major proteins expressed by M. paratuberculosis were analyzed by two-dimensional gel electrophoresis, and a superoxide dismutase (Sod) was identified from this protein profile. The M. paratuberculosis Sod has a molecular mass of 23 kDa and an isoelectric point of 6.1. Sequence analysis of the corresponding sodA gene from M. paratuberculosis indicates that this protein is a manganese-dependent enzyme. We show that the M. paratuberculosis Sod is actively secreted, suggesting that it may elicit a protective cellular immune response in the host during infection.

journal_name

FEMS Microbiol Lett

authors

Liu X,Feng Z,Harris NB,Cirillo JD,Bercovier H,Barletta RG

doi

10.1111/j.1574-6968.2001.tb10809.x

keywords:

subject

Has Abstract

pub_date

2001-08-21 00:00:00

pages

233-8

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378109701003251

journal_volume

202

pub_type

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