Purification and characteristics of cytosolic chitinase from Piromyces communis OTS1.

abstract：:A gene fusion between the Saccharomyces cerevisiae actin gene promoter and the cDNA of the Fusarium solani f. sp. pisi pelA gene has been constructed. This expression cassette has been introduced into the industrial wine yeast strain T73. The resulting recombinant strain is able to secrete active PELA enzyme into the ...

journal_title：FEMS microbiology letters

authors： González-Candelas L,Cortell A,Ramon D

更新日期：1995-03-01 00:00:00

abstract：:Among aerobic prokaryotes, many different terminal oxidase complexes have been described. Sequence comparison has revealed that the aa3-type cytochrome c oxidase and the bo3-type quinol oxidase are variations on the same theme: the heme-copper oxidase. A third member of this family has recently been recognized: the cb...

journal_title：FEMS microbiology letters

authors： van der Oost J,de Boer AP,de Gier JW,Zumft WG,Stouthamer AH,van Spanning RJ

更新日期：1994-08-01 00:00:00

abstract：:Listeria monocytogenes prfA, encoding positive regulatory factor A, is transcribed from three promoters (prfAP1, prfAP2, and PplcA). The prfAP2 promoter was previously proposed to be sigma B (sigma(B))-dependent. This hypothesis was tested by creating prfA promoter-gus transcriptional fusions in both L. monocytogenes ...

journal_title：FEMS microbiology letters

authors： Schwab U,Bowen B,Nadon C,Wiedmann M,Boor KJ

更新日期：2005-04-15 00:00:00

abstract：:We have isolated the abc1 gene from the fission yeast Schizosaccharomyces pombe. Sequence analysis suggests that the Abc1 protein is a member of the ABC superfamily of transporters and is composed of two structurally homologous halves, each consisting of a hydrophobic region of six transmembrane domains and a hydrophi...

journal_title：FEMS microbiology letters

authors： Christensen PU,Davis K,Nielsen O,Davey J

更新日期：1997-02-01 00:00:00

abstract：:Marine viruses are the most abundant biological entity in the oceans, the majority of which infect bacteria and are known as bacteriophages. Yet, the bulk of bacteriophages form part of the vast uncultured dark matter of the microbial biosphere. In spite of the paucity of cultured marine bacteriophages, it is known th...

journal_title：FEMS microbiology letters

authors： Perez Sepulveda B,Redgwell T,Rihtman B,Pitt F,Scanlan DJ,Millard A

更新日期：2016-08-01 00:00:00

abstract：:Cell-associated hemagglutination (cHA) activity with human erythrocytes was examined for 468 clinical and 71 environmental strains of Vibrio parahaemolyticus. Approximately 95% of the strains tested were cHA positive irrespective of source or Kanagawa phenomenon. 75% of clinical strains showed relatively strong mannos...

journal_title：FEMS microbiology letters

authors： Nagayama K,Oguchi T,Arita M,Honda T

更新日期：1994-07-01 00:00:00

abstract：:Arbuscular mycorrhizal fungi (AMF) have been extensively studied in natural and agricultural ecosystems, but little is known about their diversity and community structure in highly petroleum-polluted soils. In this study, we described an unexpected diversity of AMF in a sedimentation basin of a former petrochemical pl...

journal_title：FEMS microbiology letters

authors： de la Providencia IE,Stefani FO,Labridy M,St-Arnaud M,Hijri M

更新日期：2015-06-01 00:00:00

abstract：:The beta-barrel outer membrane protease Pla from Yersinia pestis is an important virulence factor in plague and enables initiation of the bubonic plague. Pla is a multifunctional protease whose expression also enhances bacterial adherence to extracellular matrix. It has remained uncertain whether the increase in cellu...

journal_title：FEMS microbiology letters

authors： Lobo LA

更新日期：2006-09-01 00:00:00

abstract：:We adapted PCR-based signature-tagged mutagenesis (STM) to Pseudomonas aeruginosa. A collection of 1056 mutants was screened in a chronic lung infection rat model. Thirteen mutants were confirmed to be attenuated. Analysis revealed that these STM mutants represented transposon insertions into eight genes previously de...

journal_title：FEMS microbiology letters

authors： Lehoux DE,Sanschagrin F,Levesque RC

更新日期：2002-04-23 00:00:00

abstract：:The enterobacterium Erwinia amylovora is the causal agent of fire blight. This study presents the analysis of the complete genome of phage PhiEaH1, isolated from the soil surrounding an E. amylovora-infected apple tree in Hungary. Its genome is 218 kb in size, containing 244 ORFs. PhiEaH1 is the second E. amylovora in...

journal_title：FEMS microbiology letters

authors： Meczker K,Dömötör D,Vass J,Rákhely G,Schneider G,Kovács T

更新日期：2014-01-01 00:00:00

abstract：:A collection of 45 epidemiologically unrelated Streptococcus agalactiae strains (group B Streptococcus, GBS), belonging to different serotypes, isolated from pregnant women in China and Russia was studied. Strains were characterized by pulsed-field gel electrophoresis (PFGE) employing hybridization with nine genes pot...

journal_title：FEMS microbiology letters

authors： Dmitriev A,Hu YY,Shen AD,Suvorov A,Yang YH

更新日期：2002-02-19 00:00:00

abstract：:Himalaya hosts a unique environment for microbial ecology. The present study aimed to explore the bioplastic producing bacterial communities along altitude gradient of Pangi-Chamba trans-Himalayan region. A total of 411 bacteria were isolated and 70 characterized at the molecular level for potential polyhydroxyalkanoa...

journal_title：FEMS microbiology letters

authors： Kumar V,Thakur V,Ambika,Kumar S,Singh D

更新日期：2018-07-01 00:00:00

abstract：:A novel strain of Rhizopus oryzae WPG secretes a noninduced lipase (ROLw) in the culture medium; purified ROLw is a protein of 29 kDa, the 45 N-terminal amino acid residues were sequenced, this sequence is very homologous to Rhizopus delemar lipase (RDL), Rhizopus niveus lipase (RNL) and R. oryzae lipase (ROL29) seque...

journal_title：FEMS microbiology letters

authors： Salah RB,Mosbah H,Fendri A,Gargouri A,Gargouri Y,Mejdoub H

更新日期：2006-07-01 00:00:00

abstract：:Expression of AT-rich genes from microorganisms such as archaea is often inefficient in Escherichia coli. The trpA and trpB genes encoding the tryptophan synthase subunits were cloned from the hyperthermophilic archaeon Pyrococcus furiosus. No apparent difference in codon bias was found between the genes. However, usi...

journal_title：FEMS microbiology letters

authors： Ishida M,Oshima T,Yutani K

更新日期：2002-11-05 00:00:00

abstract：:The gene (open reading frame Tm1155, g6pd) encoding glucose-6-phosphate dehydrogenase (G6PD, EC 1.1.1.49) of the hyperthermophilic bacterium Thermotoga maritima was cloned and functionally expressed in Escherichia coli. The purified recombinant enzyme is a homodimer with an apparent molecular mass of 95 kDa composed o...

journal_title：FEMS microbiology letters

authors： Hansen T,Schlichting B,Schönheit P

更新日期：2002-11-05 00:00:00

abstract：:Amanita exitialis Zhu L. Yang and T.H. Li is a lethal mushroom species recently isolated in Guangdong Province, China. In this report, a pure culture of this species was obtained for the first time. To confirm the identity of the pure culture, the internal transcribed spacer regions of the nuclear ribosomal DNA of the...

journal_title：FEMS microbiology letters

authors： Zhang P,Chen Z,Hu J,Wei B,Zhang Z,Hu W

更新日期：2005-11-15 00:00:00

abstract：:Streptococcus mutans, the etiologic agent of dental caries in humans, is considered a dominating force in the oral microbiome due to its highly-evolved propensity for survival. The oral pathogen encodes an elaborate array of regulatory elements, including the carbon catabolite-responsive regulator, CcpA, a global regu...

journal_title：FEMS microbiology letters

authors： Lindsay EL,Faustoferri RC,Quivey RG

更新日期：2021-01-16 00:00:00

abstract：:Acinetobacter baumannii strain A148, a clinical isolate resistant to imipenem (MIC = 32 mg l-1), synthesized two beta-lactamases with pIs 6.3 and > 9.2. The pI 6.3 enzyme hydrolyzed the penicillins, including isoxazoylpenicillins, first-, second- and, to a lesser extent, third-generation cephalosporins. It was inhibit...

journal_title：FEMS microbiology letters

authors： Hornstein M,Sautjeau-Rostoker C,Péduzzi J,Vessières A,Hong LT,Barthélémy M,Scavizzi M,Labia R

更新日期：1997-08-15 00:00:00

abstract：:Fourteen strains representing 11 Caragana-nodulating Mesorhizobium genomic species were identified as representing Mesorhizobium amorphae, Mesorhizobium huakuii, Mesorhizobium septentrionale and groups related to Mesorhizobium plurifarium, Mesorhizobium temperatum, Mesorhizobium tianshanense and Mesorhizobium mediterr...

journal_title：FEMS microbiology letters

authors： Chen WF,Guan SH,Zhao CT,Yan XR,Man CX,Wang ET,Chen WX

更新日期：2008-06-01 00:00:00

abstract：:The degradation of a series of nitroaromatic compounds by the lignin-degrading fungus Phanerochaete chrysosporium was examined. From 4-nitrotoluene (4-NT), several metabolic intermediates were identified. Initially, 4-NT was converted to 4-nitrobenzyl alcohol (4-NBA), followed by the oxidation reactions to form 4-nitr...

journal_title：FEMS microbiology letters

authors： Teramoto H,Tanaka H,Wariishi H

更新日期：2004-05-15 00:00:00

abstract：:Shigella flexneri, which causes shigellosis in humans, evolved from Escherichia coli. The sequencing of Shigella genomes has revealed that a large number of insertion sequence (IS) elements (over 200 elements) reside in the genome. Although the presence of these elements has been noted previously and summarized, more ...

journal_title：FEMS microbiology letters

authors： Zaghloul L,Tang C,Chin HY,Bek EJ,Lan R,Tanaka MM

更新日期：2007-12-01 00:00:00

abstract：:Biofilms present complex assemblies of micro-organisms attached to surfaces. they are dynamic structures in which various metabolic activities and interactions between the component cells occur. When phage come in contact with biofilms, further interactions occur dependent on the susceptibility of the biofilm bacteria...

journal_title：FEMS microbiology letters

authors： Sutherland IW,Hughes KA,Skillman LC,Tait K

更新日期：2004-03-12 00:00:00

abstract：:We report the enhanced bactericidal activity of ofloxacin in drug-containing Eudragit E100(®) dispersions (EuCl-OFX) against Pseudomonas aeruginosa and the effect of the cationic polymer on bacterial membrane. Organisms treated with EuCl-OFX showed changes in cell morphology, altered outer membrane (OM) and cytoplasm...

journal_title：FEMS microbiology letters

authors： Romero VL,Pons P,Bocco JL,Manzo RH,Alovero FL

更新日期：2012-09-01 00:00:00

abstract：:Ruminococcus albus is a Gram-positive bacterium that degrades plant cell walls in the rumen of herbivores. It was described to synthesize two major glycoside-hydrolases (Cel9B and Cel48A), which are exported and anchored at the cell surface. In bacteria, proteins destined to cross the cytoplasmic membrane are synthesi...

journal_title：FEMS microbiology letters

authors： Esbelin J,Martin C,Forano E,Mosoni P

更新日期：2009-05-01 00:00:00

abstract：:The glt operon of Escherichia coli comprises the structural genes for the glutamate synthase subunits (gltB and gltD) and gltF, whose product was previously suggested to have regulatory functions. The A/T-rich region between gltD and gltF contains a weak promoter and a translation initiation site for gltF. The GltF pr...

journal_title：FEMS microbiology letters

authors： Grassl G,Bufe B,Müller B,Rösel M,Kleiner D

更新日期：1999-10-01 00:00:00

abstract：:The effectiveness of an educational board game developed to teach the pharmacology of antimicrobial drugs to medical students was compared with the lecture-based seminar as a supplemental tool to improve short- and long-term knowledge retention and the perception of the learning method by students. A group of 124 stud...

journal_title：FEMS microbiology letters

authors： Karbownik MS,Wiktorowska-Owczarek A,Kowalczyk E,Kwarta P,Mokros Ł,Pietras T

更新日期：2016-04-01 00:00:00

abstract：:A cyclodextrin glycosyltransferase (CGTase) gene of Brevibacillus brevis CD162 was cloned into Escherichia coli using pUC19 as a vector. Determination of the nucleotide sequence showed the presence of an open reading frame of 2079 bp encoding a polypeptide of 693 amino acid residues, composed of a 20-amino acid signal...

journal_title：FEMS microbiology letters

authors： Kim MH,Sohn CB,Oh TK

更新日期：1998-07-15 00:00:00

abstract：:The distribution of virulence markers related to cytolethal distending toxin-V (CDT-V), subtilase cytotoxin (SubAB), the enterohemorrhagic Escherichia coli factor for adherence (Efa1), the adhesin similar to IrgA (Iha), the long polar fimbriae (LpfO113), the autoagglutinating adhesin (Saa), and the protein required fo...

journal_title：FEMS microbiology letters

authors： Cergole-Novella MC,Nishimura LS,Dos Santos LF,Irino K,Vaz TM,Bergamini AM,Guth BE

更新日期：2007-09-01 00:00:00

abstract：:Tachyzoites of Toxoplasma gondii multiply within the parasitophorous vacuole (PV) until the lysis of the host cell. This study was undertaken to evaluate the effect of hydroxyurea (a specific drug that arrests cell division at G1/S phase) on the multiplication of T. gondii tachyzoites in infected Vero cells. Infected ...

journal_title：FEMS microbiology letters

authors： de Melo EJ,Mayerhoffer RO,de Souza W

更新日期：2000-04-01 00:00:00

abstract：:Type II restriction endonucleases were purified by heparin-sepharose followed by ion chromatography from Treponema strains. The results indicate that in addition to frequently cutting GATC-specific restriction enzymes, the tested strains also possess rarely cutting endonucleases. The purified restriction endonucleases...

journal_title：FEMS microbiology letters

authors： Piknova M,Javorsky P,Pristas P

更新日期：2005-10-01 00:00:00