Multiple restriction-modification systems are present in rumen treponemes.

Abstract:

:Type II restriction endonucleases were purified by heparin-sepharose followed by ion chromatography from Treponema strains. The results indicate that in addition to frequently cutting GATC-specific restriction enzymes, the tested strains also possess rarely cutting endonucleases. The purified restriction endonucleases represent four different sequence specificities, comprising isoschizomers of DrdI, AflII, Tth111I and NdeI. The data presented show that three rumen Treponema strains possess altogether seven type II restriction-modification systems. Thus, individual Treponema strains may be considered an interesting source of multiple type II restriction enzymes.

journal_name

FEMS Microbiol Lett

authors

Piknova M,Javorsky P,Pristas P

doi

10.1016/j.femsle.2005.07.032

keywords:

subject

Has Abstract

pub_date

2005-10-01 00:00:00

pages

99-103

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(05)00521-5

journal_volume

251

pub_type

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