Abstract：
:Two isolates, belonging to a new species of a novel genus of the Phylum "Deinococcus/Thermus ", were recovered from hot spring runoffs on the Island of São Miguel in the Azores. Strains RQ-24(T) and TU-8 are the first cultured representatives of a distinct phylogenetic lineage within this phylum. These strains form orange/red colonies, spherical-shaped cells, have an optimum growth temperature of about 50 degrees C, an optimum pH for growth between about 7.5 and 9.5, and do not grow at pH below 6.5 or above pH 11.2. These organisms grow in complex media without added NaCl, but have a maximum growth rate in media with 1.0% NaCl and grow in media containing up to 6.0% NaCl. The organisms are extremely ionizing radiation resistant; 60% of the cells survive 5.0 kGy. These strains are chemoorganotrophic and aerobic; do not grow in Thermus medium under anaerobic conditions with or without nitrate as electron acceptor and glucose as a source of carbon and energy, but ferment glucose to D-lactate without formation of gas. The organisms assimilate a large variety of sugars, organic acids and amino acids. Fatty acids are predominantly iso- and anteiso-branched; long chain 1,2 diols were also found in low relative proportions; menaquinone 8 (MK-8) is the primary respiratory quinone. Peptidoglycan was not detected. Based on 16S rRNA gene sequence analysis, physiological, biochemical and chemical analysis we describe a new species of one novel genus represented by strain RQ-24(T) (CIP 108686(T)=LMG 22925(T)=DSM 17093(T)) for which we propose the name Truepera radiovictrix. We also propose the family Trueperaceae fam. nov. to accommodate this new genus.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Albuquerque L,Simões C,Nobre MF,Pino NM,Battista JR,Silva MT,Rainey FA,da Costa MSdoi
10.1016/j.femsle.2005.05.002keywords:
subject
Has Abstractpub_date
2005-06-15 00:00:00pages
161-9issue
2eissn
0378-1097issn
1574-6968pii
S0378-1097(05)00284-3journal_volume
247pub_type
杂志文章abstract：:In this report we describe the development of a highly stringent and dually regulated promoter system for Shigella flexneri. Dual regulation was provided by utilizing a promoter susceptible to control by the bacteriophage P1 temperature-sensitive C1 repressor that in turn was under the transcriptional control of LacI....
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2002.tb11396.x
更新日期：2002-10-08 00:00:00
abstract：:Halobacillus trueperi accumulates glycine betaine under condition of high osmolarity. A fragment of the glycine betaine transporter betH gene was obtained from the genome of H. trueperi with degenerate primers. Through Southern blot hybridization and inverse PCR, a 5.1 kb EcoRI fragment containing the complete betH ge...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/j.femsle.2004.05.015
更新日期：2004-06-15 00:00:00
abstract：:The stable carbon isotopic compositions of the inorganic carbon source, bulk cell material, and isoprenoid lipids of the hyperthermophilic crenarchaeon Metallosphaera sedula, which uses a 3-hydroxypropionate-like pathway for autotrophic carbon fixation, have been measured. Bulk cell material was approximately 3 per th...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2001.tb10542.x
更新日期：2001-03-01 00:00:00
abstract：:Acetobacter spp. are used for industrial vinegar production because of their high ability to oxidize ethanol to acetic acid and high resistance to acetic acid. Two-dimensional gel electrophoretic analysis of a soluble fraction of Acetobacter aceti revealed the presence of several proteins whose production was enhanced...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/j.femsle.2004.05.007
更新日期：2004-06-15 00:00:00
abstract：:In this study, a putative N-acetylmuramyl-l-alanine amidase gene (bb0666) was identified in the genome of the Lyme disease spirochete Borrelia burgdorferi. This protein shares c. 30% identity with its counterparts from other bacteria. Reverse transcriptase-PCR analysis showed that bb0666 along with two other genes (bb...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2008.01416.x
更新日期：2009-01-01 00:00:00
abstract：:A successful Staphylococcus aureus vaccine should elicit a long-term antibody response that prevents establishment of the infection. The aim of the present study was to evaluate the functional role of antibodies raised against different S. aureus CP5 vaccines in invasion to bovine mammary epithelial cells (MAC-T) and ...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/1574-6968.12588
更新日期：2014-11-01 00:00:00
abstract：:Three different whole cell biosensor constructs were made by fusing the mercury inducible promoter, P(mer), and its regulatory gene, merR, from transposon Tn21 with reporter genes luxCDABE, lacZYA, or gfp. In Escherichia coli these biosensor constructs responded to low levels of mercury by producing light, beta-galact...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2000.tb09413.x
更新日期：2000-12-01 00:00:00
abstract：:The accumulation of 2-keto-3-deoxy-6-phosphogluconate, the key intermediate of the Entner-Doudoroff pathway, has long been thought to inhibit growth of bacteria, but careful measurements of 2-keto-3-deoxy-6-phosphogluconate accumulation by growing cells and the correlation of intracellular 2-keto-3-deoxy-6-phosphogluc...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1998.tb12870.x
更新日期：1998-02-15 00:00:00
abstract：:In order to characterize the metabolites produced in vivo by biphenyl-2,3-dioxygenase and biphenyl-2,3-dihydrodiol-2,3-dehydrogenase, the first two enzymes of the (polychloro)biphenyl catabolic pathway encoded by the bph locus of Pseudomonas sp. LB400, recombinant E. coli strains expressing the respective genes were c...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1995.tb07894.x
更新日期：1995-11-15 00:00:00
abstract：:A number of hybrids obtained by fusion of protoplasts of complementary auxotrophic strains of Candida albicans were analysed for growth rates, cell volume, DNA content, stability and adherence to human buccal epithelial cells. SDS-PAGE analysis of the cell wall proteins indicated that the hybrid cell walls contained m...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1994.tb06617.x
更新日期：1994-01-01 00:00:00
abstract：:The gene encoding the alpha-subunit of methanol dehydrogenase (mxaF) and its flanking region was isolated from a methylotrophic bacterium, Hyphomicrobium methylovorum GM2. The deduced amino acid sequence of MxaF showed 80, 80, 74 and 66% identity with those of Methylobacterium extorquens AM1, M. organophilum XX, Parac...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1997.tb12673.x
更新日期：1997-09-15 00:00:00
abstract：:The central carbon metabolism is well investigated in bacteria, but this is not the case for archaea. MJ0400-His(6) from Methanocaldococcus jannaschii catalyzes the cleavage of fructose-1,6-bisphosphate (FBP) to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate with a V(max) of 33 mU mg(-1) and a K(m) of 430 m...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2008.01079.x
更新日期：2008-04-01 00:00:00
abstract：:The filamentous fungus Cunninghamella elegans has the ability to metabolize xenobiotics, including polycyclic aromatic hydrocarbons and pharmaceutical drugs, by both phase I and II biotransformations. Cytosolic and microsomal fractions were assayed for activities of cytochrome P450 monooxygenase, aryl sulfotransferase...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1996.tb08161.x
更新日期：1996-05-01 00:00:00
abstract：:Using electron microscopy, pili with at least two distinct morphologies were observed on strains of Pasteurella multocida isolated from pigs with atrophic rhinitis. Rigid pili were found on 60-80% of all cells observed. These pili had a strong tendency to lie flat along the side of the outer cell membrane of P. multoc...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/0378-1097(95)00317-x
更新日期：1995-10-15 00:00:00
abstract：:An identification method by PCR, specific to the Carnobacterium genus, was optimised by testing it on 28 bacterial strains. Primers from the literature were tested to differentiate Carnobacterium strains present among various bacterial species. The DNA of Carnobacterium species (C. alterfunditum, C. divergens, C. fund...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/j.femsle.2005.05.037
更新日期：2005-09-01 00:00:00
abstract：:Lactic acid bacteria (LAB) have a long history in industrial processes as food starters and biocontrol agents, and also as producers of high-value compounds. Lactic acid, their main product, is among the most requested chemicals because of its multiple applications, including the synthesis of biodegradable plastic pol...
journal_title：FEMS microbiology letters
pub_type： 杂志文章,评审
doi：10.1093/femsle/fny126
更新日期：2018-08-01 00:00:00
abstract：:Saccharomyces cerevisiae strains tolerant to osmotic stress are important for ethanol production during very high gravity (VHG) fermentation. We aimed to identify novel genes that confer enhanced tolerance to osmotic stress in S. cerevisiae. Two strains tolerant to up to 30% (w/v) glucose were isolated by screening a ...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1093/femsle/fny149
更新日期：2018-07-01 00:00:00
abstract：:The Mycobacterium marinum G13 promoter is a sigma 70-like promoter that is more active by green fluorescent protein (gfp) differential fluorescence induction (DFI) assays when M. marium resides in an intracellular compartment as compared with growth in media. In assays using DFI, we found that the mycobacterial G13 pr...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2005.00078.x
更新日期：2006-02-01 00:00:00
abstract：:Astaxanthin possesses higher antioxidant activity than other carotenoids and, for this and other reasons, has great commercial potential for use in the aquaculture, pharmaceutical, and food industries. The basidiomycetous yeast Xanthophyllomyces dendrorhous is one of the best natural producers of astaxanthin, but wild...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2008.01278.x
更新日期：2008-09-01 00:00:00
abstract：:The effects of specific inhibitors of glycoprotein trimming reactions on Junin virus (JV) replication were investigated. Bromoconduritol, an inhibitor of glucosidase II, significantly reduced infective virus production (DE50: 1.1 mM) and viral protein expression. Neither 1-deoxynojirimycin, an inhibitor of both glucos...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1993.tb06140.x
更新日期：1993-05-01 00:00:00
abstract：:Rainbow trout gastroenteritis has been related to the accumulation of segmented filamentous bacteria in the digestive tract of fish, which presents lethargy, reduced appetite and accumulation of mucoid faeces. Some authors associate the comparison of illness with the presence of viable filaments, which produce and rel...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2010.01984.x
更新日期：2010-07-01 00:00:00
abstract：:The study compared images of mature Streptococcus mutans biofilms captured at increasing magnification to determine which microscopy method is most acceptable for imaging the biofilm topography and the extracellular polymeric substance (EPS). In vitro S. mutans biofilms were imaged using (1) scanning electron microsco...
journal_title：FEMS microbiology letters
pub_type： 信件
doi：10.1111/1574-6968.12334
更新日期：2014-01-01 00:00:00
abstract：:micF RNA, whose sequence is highly complementary to a 5'-portion of ompF mRNA, has been implicated in the osmoregulation and thermoregulation of the ompF porin gene in Escherichia coli. To define and characterize cis-acting regulatory regions upstream of the micF promoter, a series of deletions of the micF promoter fu...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/0378-1097(91)90440-l
更新日期：1991-09-15 00:00:00
abstract：:Primers designed to conserved regions of botulinum and tetanus clostridial toxins were used to amplify DNA fragments from non-proteolytic Clostridium botulinum type F (202F) DNA using polymerase chain reaction technology. The fragments were cloned and the complete nucleotide sequence of the gene encoding type F toxin ...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/0378-1097(92)90408-g
更新日期：1992-09-15 00:00:00
abstract：:Adenylate kinase isoenzymes localised in the mitochondria and in the cytosol have been detected in extracts of glucose-grown Aspergillus nidulans using specific staining after electrophoresis on cellulose acetate. The isoenzymes have similar Km values for AMP, ADP and MgATP2- but may differ in the mechanism used for i...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/0378-1097(91)90146-2
更新日期：1991-03-01 00:00:00
abstract：:Neisseria gonorrhoeae and Neisseria meningitidis are exclusively human pathogens. A crucial property of the pathogenicity of neisserial infection is the ability to adhere to human epithelial cells. Pili mediate adherence of these bacteria to target cells and thereby promote colonization and infection of mucosal surfac...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1998.tb12974.x
更新日期：1998-05-01 00:00:00
abstract：:Eight-day-old embryonated hen's eggs were used as a model to study Mycobacterium avium virulence. Strains isolated from human patients caused 20-90% mortality when eggs were infected by injection of bacterial suspensions into the amniotic sac. Virulence of examined strains subsequently decreased with passage through e...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2000.tb09297.x
更新日期：2000-09-15 00:00:00
abstract：:A novel bacterial strain, PM2, capable of growing on methanol, was isolated in alkaline conditions from a soil inoculum. This bacterium was characterized at the physiological, biochemical and molecular level. Based on biochemical and molecular data strain PM2 was classified as a novel member of the group of fluorescen...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/S0378-1097(03)00692-X
更新日期：2003-10-24 00:00:00
abstract：:The flaA gene, which codes for the Spirochaeta aurantia flagellar filament outer layer polypeptide, FlaA, was cloned, sequenced and analysed. The gene appears to be transcribed into a monocistronic mRNA from a sigma 70-like promoter. The translational start is 31 base pairs after the start of the transcript, the open ...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1993.tb05971.x
更新日期：1993-02-01 00:00:00
abstract：:A protein kinase from Dictyostelium discoideum which phosphorylates the synthetic peptide, calmodulin-dependent protein kinase substrate (CDPKS, amino acid sequence: PLRRTLSVAA) and is stimulated by Ca2+/calmodulin is described. This is the first report of a protein kinase with these characteristics in D. discoideum. ...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1994.tb06623.x
更新日期：1994-01-01 00:00:00
