Abstract:
:Astaxanthin possesses higher antioxidant activity than other carotenoids and, for this and other reasons, has great commercial potential for use in the aquaculture, pharmaceutical, and food industries. The basidiomycetous yeast Xanthophyllomyces dendrorhous is one of the best natural producers of astaxanthin, but wild-type cells accumulate only a small amount of astaxanthin. In this study, we developed an efficient flow cytometry method to screen for astaxanthin-overproducing mutants of X. dendrorhous. We first examined the relationship between cellular astaxanthin content and the intensity of fluorescence emitted from the cell. Although the fluorescence emission maximum of astaxanthin dissolved in acetone occurred at 570 nm, intracellular astaxanthin content correlated better with emission at around 675 nm in different X. dendrorhous strains. Using this emission wavelength, we screened cells mutagenized with ethyl methanesulfonate and successfully isolated mutants that produced 1.5-3.8-fold more astaxanthin than parent cells. This method enabled us to obtain overproducers five times more efficient than conventional screening from plate culture.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Ukibe K,Katsuragi T,Tani Y,Takagi Hdoi
10.1111/j.1574-6968.2008.01278.xsubject
Has Abstractpub_date
2008-09-01 00:00:00pages
241-8issue
2eissn
0378-1097issn
1574-6968pii
FML1278journal_volume
286pub_type
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