Efficient screening for astaxanthin-overproducing mutants of the yeast Xanthophyllomyces dendrorhous by flow cytometry.


:Astaxanthin possesses higher antioxidant activity than other carotenoids and, for this and other reasons, has great commercial potential for use in the aquaculture, pharmaceutical, and food industries. The basidiomycetous yeast Xanthophyllomyces dendrorhous is one of the best natural producers of astaxanthin, but wild-type cells accumulate only a small amount of astaxanthin. In this study, we developed an efficient flow cytometry method to screen for astaxanthin-overproducing mutants of X. dendrorhous. We first examined the relationship between cellular astaxanthin content and the intensity of fluorescence emitted from the cell. Although the fluorescence emission maximum of astaxanthin dissolved in acetone occurred at 570 nm, intracellular astaxanthin content correlated better with emission at around 675 nm in different X. dendrorhous strains. Using this emission wavelength, we screened cells mutagenized with ethyl methanesulfonate and successfully isolated mutants that produced 1.5-3.8-fold more astaxanthin than parent cells. This method enabled us to obtain overproducers five times more efficient than conventional screening from plate culture.


FEMS Microbiol Lett


Ukibe K,Katsuragi T,Tani Y,Takagi H




Has Abstract


2008-09-01 00:00:00














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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jock S,Völksch B,Mansvelt L,Geider K

    更新日期:2002-06-04 00:00:00

  • Functional conservation between the argininosuccinate lyase of the archaeon Methanococcus maripaludis and the corresponding bacterial and eukaryal genes.

    abstract::The argH gene encoding argininosuccinate lyase (ASL) of Methanococcus maripaludis was cloned on a 4.7-kb HindIII genomic fragment. The gene is preceded by a short open reading frame (ORF149), which encodes a polypeptide with an unknown function. The two genes are co-transcribed. The ASL of M. maripaludis shares a high...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cohen-Kupiec R,Kupiec M,Sandbeck K,Leigh JA

    更新日期:1999-04-01 00:00:00

  • Accelerated methanogenesis from aliphatic and aromatic hydrocarbons under iron- and sulfate-reducing conditions.

    abstract::The impact of four electron acceptors on hydrocarbon-induced methanogenesis was studied. Methanogenesis from residual hydrocarbons may enhance the exploitation of oil reservoirs and may improve bioremediation. The conditions to drive the rate-limiting first hydrocarbon-oxidizing steps for the conversion of hydrocarbon...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Siegert M,Cichocka D,Herrmann S,Gründger F,Feisthauer S,Richnow HH,Springael D,Krüger M

    更新日期:2011-02-01 00:00:00

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    abstract::A real-time nucleic acid sequence-based amplification assay, targeting tmRNA, was designed for the rapid identification of Staphylococcus aureus. The selectivity of the assay was confirmed against a panel of 76 Staphylococcus strains and species and 22 other bacterial species. A detection limit of 1 cell equivalent wa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: O'Grady J,Lacey K,Glynn B,Smith TJ,Barry T,Maher M

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dolfing J

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mukoyama D,Takeyama H,Kondo Y,Matsunaga T

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Bonner TJ,Pell JK,Gray SN

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    pub_type: 杂志文章


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    pub_type: 杂志文章,评审


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Thaller MC,Schippa S,Bonci A,Cresti S,Rossolini GM

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Maggi T,Spinosa M,Ricci S,Medaglini D,Pozzi G,Oggioni MR

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