Abstract:
:While there is an extensive database of genes encoding ammonia monooxygenase (amo) found in the ammonia-oxidizing beta-proteobacteria, few amo sequences are available representing the gamma-proteobacteria. We sequenced the complete amo operon (amoCAB) for Nitrosococcus oceani (ATCC 19707), a marine, autotrophic, ammonia-oxidizing bacterium belonging to the gamma-subdivision of the proteobacteria. An additional autotrophic, ammonia-oxidizing bacterium isolated from a marine environment (strain C-113) was identified as belonging to the Nitrosococcus genus by 16S rDNA analysis and its amo operon was sequenced. This is the first report of a full-length sequence for the amo operon from a gamma-subdivision autotrophic ammonia-oxidizing bacterium. The N. oceani and C-113 amo genes were 88-90% identical to each other, 49-53% identical to the pmo genes encoding the related particulate methane monooxygenase of Methylococcus capsulatus (Bath), and 39-42% identical to the amo genes of the beta-subdivision autotrophic ammonia-oxidizing bacteria. In both Nitrosococcus strains, the amo operon was found as a single copy and contained three genes, amoC, amoA, amoB, with intergenic spacer regions between amoC and amoA (286 bp) and between amoA and amoB (65 bp). We conclude that the amo genes will allow for a finer scale phylogenetic differentiation than 16S rDNA within the gamma-subdivision AOB.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Alzerreca JJ,Norton JM,Klotz MGdoi
10.1111/j.1574-6968.1999.tb08773.xkeywords:
subject
Has Abstractpub_date
1999-11-01 00:00:00pages
21-9issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(99)00457-7journal_volume
180pub_type
杂志文章abstract::Acinetobacter sp. strain 20B was isolated based on the ability to utilize dimethyl sulfide as the sole sulfur source. Since strain 20B oxidized indole as well as dimethyl sulfide, indigo production by recombinant Escherichia coli clones carrying Acinetobacter DNA was used as a selection for cloning genes encoding dime...
journal_title:FEMS microbiology letters
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doi:10.1111/j.1574-6968.1997.tb12692.x
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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更新日期:2002-12-17 00:00:00
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journal_title:FEMS microbiology letters
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更新日期:2004-08-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1995.tb07731.x
更新日期:1995-08-01 00:00:00
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journal_title:FEMS microbiology letters
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更新日期:2005-09-15 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:1998-12-15 00:00:00
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:1999-05-01 00:00:00
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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journal_title:FEMS microbiology letters
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更新日期:1994-10-15 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:2001-11-13 00:00:00
abstract::The variable stress-sensitivity of individual cells within pure cultures is widely noted but generally unexplained. Here, factors determining the heterogeneous susceptibility to copper toxicity in Saccharomyces cerevisiae were examined with a rapid non-perturbing approach based on flow cytometry. By determination of t...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1999.tb13687.x
更新日期:1999-07-15 00:00:00