Cell wall substrate specificity of six different lysozymes and lysozyme inhibitory activity of bacterial extracts.

abstract：:The branched-chain amino acid permease Bap2p is a transport system for leucine, isoleucine, and valine in Saccharomyces cerevisiae, and its synthesis is regulated transcriptionally. However, the downregulation mechanisms of Bap2p have not been established. Here we demonstrate that the C-terminal region of Bap2p plays ...

journal_title：FEMS microbiology letters

authors： Omura F,Kodama Y,Ashikari T

更新日期：2001-01-15 00:00:00

abstract：:Periodic evolution of H(2)S during aerobic chemostat culture of Saccharomyces cerevisiae resulted in ultradian metabolic oscillation via periodic inhibition of respiratory activity. To understand the nature of periodic H(2)S evolution, we investigated whether oxidative stress is associated with H(2)S production. The c...

journal_title：FEMS microbiology letters

authors： Kwak WJ,Kwon GS,Jin I,Kuriyama H,Sohn HY

更新日期：2003-02-14 00:00:00

abstract：:D-(-)-3-Hydroxybutyrate (3HB) oligomer hydrolase was purified from Paracoccus denitrificans. The enzyme was a monomeric protein with an approximate molecular mass of 31 kDa. The isoelectric point of the enzyme was 5.2. Optimum temperature and pH were 35-40 degrees C and 8.0, respectively. The enzyme activity was not a...

journal_title：FEMS microbiology letters

authors： Ueda S,Sano K,Gao D,Tomihari N,Yamane T,Endo I

更新日期：2002-01-10 00:00:00

abstract：:L-allo-Threonine aldolase (L-allo-threonine acetaldehyde-lyase), which exhibited specificity for L-allo-threonine but not for L-threonine, was purified from a cell-free extract of Aeromonas jandaei DK-39. The purified enzyme catalyzed the aldol cleavage reaction of L-allo-threonine (K(m) = 1.45 mM, Vmax = 45.2 mumol m...

journal_title：FEMS microbiology letters

authors： Kataoka M,Wada M,Nishi K,Yamada H,Shimizu S

更新日期：1997-06-15 00:00:00

abstract：:The genome size of Actinobacillus pleuropneumoniae was determined by pulsed field gel electrophoresis of AscI and ApaI digested chromosomal DNA. The genome size of the type strain 4074T (serotype 1) was determined to be 2404 +/- 40 kb. The chromosome sizes for the reference strains of the other serotypes range between...

journal_title：FEMS microbiology letters

authors： Chevallier B,Dugourd D,Tarasiuk K,Harel J,Gottschalk M,Kobisch M,Frey J

更新日期：1998-03-15 00:00:00

abstract：:We have investigated the mechanisms used by Trichomonas vaginalis to damage cellular membranes, using human erythrocytes as target cells. Haemolysis is a contact- and temperature-dependent phenomenon, and is inhibited in 4 mM EGTA. Osmotic protection experiments using carbohydrates with different molecular diameters a...

journal_title：FEMS microbiology letters

authors： Fiori PL,Rappelli P,Rocchigiani AM,Cappuccinelli P

更新日期：1993-05-01 00:00:00

abstract：:CheY, the response regulator of the chemotaxis system in Escherichia coli, can be regulated by two covalent modifications - phosphorylation and acetylation. Both covalent modifications are involved in chemotaxis, but the mechanism and role of the acetylation are still obscure. While acetylation was shown to repress th...

journal_title：FEMS microbiology letters

authors： Li R,Chen P,Gu J,Deng JY

更新日期：2013-10-01 00:00:00

abstract：:A previous study [DeVault et al. (1991) Mol. Microbiol. 5, 2503-2509] suggested that growth of Pseudomonas aeruginosa in glucose-containing medium represses algD gene transcription. In this study, growth of P. aeruginosa in rich medium containing glucose or gluconate increased alginate production and algD transcriptio...

journal_title：FEMS microbiology letters

authors： Ma JF,Phibbs PV,Hassett DJ

更新日期：1997-03-15 00:00:00

abstract：:A microorganism which reduces Fe(III) during the fermentation of glucose was isolated from freshwater sediment. The Fe(III) was supplied to enrichment cultures as a soluble complex with the bidentate ligand maltol (3-hydroxy-2-methyl-4-pyrone). Advantages that were afforded by the use of Fe(III)(maltol)3 over previous...

journal_title：FEMS microbiology letters

authors： Dobbin PS,Carter JP,Garcia-Salamanca San Juan C,von Hobe M,Powell AK,Richardson DJ

更新日期：1999-07-01 00:00:00

abstract：:Fourteen strains representing 11 Caragana-nodulating Mesorhizobium genomic species were identified as representing Mesorhizobium amorphae, Mesorhizobium huakuii, Mesorhizobium septentrionale and groups related to Mesorhizobium plurifarium, Mesorhizobium temperatum, Mesorhizobium tianshanense and Mesorhizobium mediterr...

journal_title：FEMS microbiology letters

authors： Chen WF,Guan SH,Zhao CT,Yan XR,Man CX,Wang ET,Chen WX

更新日期：2008-06-01 00:00:00

abstract：:Proteins which are secreted or associated with the cell envelope of Mycobacterium tuberculosis may contain protective T-cell epitopes. Prior to this study, a recombinant clone bank of enzymatically active M. tuberculosis-alkaline phosphatase fusions, were screened for immunogenicity in a murine T-cell model. Five of t...

journal_title：FEMS microbiology letters

authors： Moran AJ,Doran JL,Wu J,Treit JD,Ekpo P,Kerr VJ,Roberts AD,Orme IM,Galant S,Ress SR,Nano FE

更新日期：1999-08-01 00:00:00

abstract：:Reduction of D-fructose to D-mannitol by whole-cell biotransformation with recombinant resting cells of Corynebacterium glutamicum ATCC13032 requires the coexpression of mdh and fdh, which encode mannitol and formate dehydrogenases, respectively. However, d-mannitol formation is limited by the uptake of d-fructose in ...

journal_title：FEMS microbiology letters

authors： Bäumchen C,Krings E,Bringer S,Eggeling L,Sahm H

更新日期：2009-01-01 00:00:00

abstract：:To investigate the auto-ecology of a strain of Gigaspora margarita in a commercial inoculum, we found a pair of PCR primers amplifying a sequence of 235 bp diagnostic of the isolate. We designed an oligonucleotide probe based on the DNA sequence. The combination of PCR and the probing successfully detected the diagnos...

journal_title：FEMS microbiology letters

authors： Yokoyama K,Tateishi T,Marumoto T,Saito M

更新日期：2002-07-02 00:00:00

abstract：:Streptococcus suis (S. suis) is an emerging zoonotic agent that exhibits high level resistance to classic antibiotics and a heavy burden in the swine industry. Therefore alternative antibacterial agents need to be developed. A novel endolysin derived from the S. suis temperate phage phi5218, termed Ply5218, was identi...

journal_title：FEMS microbiology letters

authors： Zhang H,Zhang C,Wang H,Yan YX,Sun J

更新日期：2016-09-01 00:00:00

abstract：:A protease (protease A) was successfully purified from the extracellular proteins of Vibrio parahaemolyticus no. 93, a clinical strain carrying neither tdh nor trh genes, using phenyl-Sepharose CL-4B hydrophobic interaction chromatography. The molecular mass of protease A was 43 kDa using gel filtration, which was in ...

journal_title：FEMS microbiology letters

authors： Lee CY,Cheng MF,Yu MS,Pan MJ

更新日期：2002-03-19 00:00:00

abstract：:pSA1.1 is a 9.1-kb multicopy plasmid originally isolated from Streptomyces cyaneus (formerly S. azureus) ATCC 14921. This plasmid accumulates single-stranded DNA in S. lividans and is therefore considered to replicate by a rolling-circle replication. In the present work, the rep gene encoding the replication initiator...

journal_title：FEMS microbiology letters

authors： Yokoyama E,Matsuzaki Y,Doi K,Ogata S

更新日期：1998-12-01 00:00:00

abstract：:A cyclodextrin glycosyltransferase (CGTase) gene of Brevibacillus brevis CD162 was cloned into Escherichia coli using pUC19 as a vector. Determination of the nucleotide sequence showed the presence of an open reading frame of 2079 bp encoding a polypeptide of 693 amino acid residues, composed of a 20-amino acid signal...

journal_title：FEMS microbiology letters

authors： Kim MH,Sohn CB,Oh TK

更新日期：1998-07-15 00:00:00

abstract：:A growing body of evidence implicates streptococcal and staphylococcal superantigens in the development of psoriasis, atopic dermatitis and Kawasaki syndrome. In each of these illnesses, an abnormal state of immunologic activity is observed. Superantigens, which have a unique ability to activate large numbers of lymph...

journal_title：FEMS microbiology letters

authors： Yarwood JM,Leung DY,Schlievert PM

更新日期：2000-11-01 00:00:00

abstract：:A semi-automated method has been developed for the quantification and measurement of conidia discharged by the aphid pathogen Erynia neoaphidis. This was used to compare conidiation by E. neoaphidis-mycosed pea aphid cadavers, mycelial plugs cut from agar plates, mycelial pellets from shake flasks and by mycelial pell...

journal_title：FEMS microbiology letters

authors： Bonner TJ,Pell JK,Gray SN

更新日期：2003-03-14 00:00:00

abstract：:The cryopreservation of an anaerobic rumen fungus, Piromyces communis OTS1, was examined at -84 degrees C using dimethyl sulfoxide, propylene glycol or ethylene glycol as cryoprotectants. Ethylene glycol was the most effective agent, combining high survival and low toxicity, followed by dimethyl sulfoxide and propylen...

journal_title：FEMS microbiology letters

authors： Sakurada M,Tsuzuki Y,Morgavi DP,Tomita Y,Onodera R

更新日期：1995-04-01 00:00:00

abstract：:The shaA gene (sodium-hydrogen antiporter gene A, identical to mrpA) is largely responsible for Na+ extrusion in Bacillus subtilis. The disruption of shaA combined with a low concentration of NaCl completely abolishes sporulation but allows normal growth. To investigate the role of shaA and shaA-mediated sodium ion ho...

journal_title：FEMS microbiology letters

authors： Kosono S,Asai K,Sadaie Y,Kudo T

更新日期：2004-03-12 00:00:00

abstract：:Acinetobacter sp. strain 20B was isolated based on the ability to utilize dimethyl sulfide as the sole sulfur source. Since strain 20B oxidized indole as well as dimethyl sulfide, indigo production by recombinant Escherichia coli clones carrying Acinetobacter DNA was used as a selection for cloning genes encoding dime...

journal_title：FEMS microbiology letters

authors： Horinouchi M,Kasuga K,Nojiri H,Yamane H,Omori T

更新日期：1997-10-01 00:00:00

abstract：:Binding of meningitis-causing Escherichia coli K1 to human brain microvascular endothelial cells (HBMEC) contributes to traversal of the blood-brain barrier, which occurs in part by the mannose-sensitive binding of FimH. In this study, we showed that FimH also binds to HBMEC, independent of mannose, and identified ATP...

journal_title：FEMS microbiology letters

authors： Shin S,Kim KS

更新日期：2010-02-01 00:00:00

abstract：:The enterotoxigenic Escherichia coli (ETEC) strain Ec2173, causing post weaning diarrhoea in swine, harbours six plasmids ranging from 13 to 200 kb in size. The heat stable toxin genes sta, stb and a tetracycline resistance gene were located on a self conjugative 120-kb plasmid, called pTC. In the cloned ColE1 type or...

journal_title：FEMS microbiology letters

authors： Olasz F,Fekete PZ,Blum-Oehler G,Boldogkoi Z,Nagy B

更新日期：2005-03-15 00:00:00

abstract：:Enterococcus faecium, a major cause of nosocomial infections, is often isolated from conditions where biofilm is considered to be important in the establishment of infections. We investigated biofilm formation among E. faecium isolates from diverse sources and found that the occurrence and amount of biofilm formation ...

journal_title：FEMS microbiology letters

authors： Almohamad S,Somarajan SR,Singh KV,Nallapareddy SR,Murray BE

更新日期：2014-04-01 00:00:00

abstract：:Candida albicans yeasts adhered avidly to extracellular matrix (ECM) proteins, type IV collagen, laminin, and fibronectin immobilized on plastic. Type IV collagen showed an increase of adherence of 400% above control values; laminin, 300%; and fibronectin, 150%. In addition, all three (in quantities of 0.02-200 microg...

journal_title：FEMS microbiology letters

authors： Klotz SA

更新日期：1990-03-15 00:00:00

abstract：:We have constructed a Thermus promoter probe vector pPP11 containing the promoterless heat-stable kanamycin resistance (Kmr) gene. Total DNA of T. thermophilus HB27 was randomly sheared into 100-200 bp fragments and inserted into the EcoRI site of pPP11. About 1000 clones which showed Kmr were obtained, and they were ...

journal_title：FEMS microbiology letters

authors： Maseda H,Hoshino T

更新日期：1995-05-01 00:00:00

abstract：:Biological halogenation of aromatic compounds implies the generation of reducing equivalents in the form of e.g. NADH. Thermodynamic calculations show that coupling the halogenation step to a step in which the reducing equivalents are oxidized with a potent oxidant such as O2 or N2O makes the halogenation reaction the...

journal_title：FEMS microbiology letters

authors： Dolfing J

更新日期：1998-10-15 00:00:00

abstract：:Outer membrane-associated proteins of 18 kDa were expressed by enteroaggregative Escherichia coli (EAggEC) belonging to serotypes O126:H27 and O44:H18, which hybridized with a probe derived from a plasmid necessary for enteroaggregative adhesion. The 18 kDa proteins expressed by strains of E. coli, belonging to these ...

journal_title：FEMS microbiology letters

authors： Chart H,Smith HR,Rowe B

更新日期：1995-10-01 00:00:00

abstract：:Emergence of races in Fusarium oxysporum f. sp. lycopersici (Fol) is caused by loss or mutation of at least one avirulence (AVR) gene. The product of AVR1 is a small protein (Avr1) secreted by Fol in tomato xylem sap during infection. This protein triggers Fol race 1 specific resistance (I) in tomato, indicating that ...

journal_title：FEMS microbiology letters

authors： Kashiwa T,Suzuki T,Sato A,Akai K,Teraoka T,Komatsu K,Arie T

更新日期：2016-07-01 00:00:00