Purification and characterization of a putative virulence factor, serine protease, from Vibrio parahaemolyticus.


:A protease (protease A) was successfully purified from the extracellular proteins of Vibrio parahaemolyticus no. 93, a clinical strain carrying neither tdh nor trh genes, using phenyl-Sepharose CL-4B hydrophobic interaction chromatography. The molecular mass of protease A was 43 kDa using gel filtration, which was in agreement with the results obtained from SDS-PAGE, suggesting that protease A was a monomeric protein. Additionally, the isoelectric point of this protein was 5.0. The optimum temperature and pH of protease A ranged from 40 degrees C to 50 degrees C and pH 8, respectively. Protease A activity was inhibited by serine protease inhibitors, such as phenylmethylsulfonyl fluoride and soybean trypsin inhibitor; moreover, the activity could be blocked by treatment with 20 mM of 1,10-phenanthroline, but could not be restored by adding metal ions. These results indicated that protease A is a serine protease that requires metal. The 12 N-terminal residues of protease A showed a high degree of identity (81%) to the sequence of Vibrio metschnikovii VapT serine protease. The purified protease had significant effects on the growth of Chinese hamster ovary, HeLa, Vero and Caco-2 cells and its cytotoxic activity was not blocked by gangliosides. Protease A lysed erythrocytes well but its hemolytic activity was unstable after heat treatment, indicating that protease A is able to cause hemolysis but is a heat-labile protein. The purified protease caused tissue hemorrhage and death in mice when injected both intraperitoneally and intravenously. In conclusion, this is the first report of a serine protease purified directly from the supernatant of V. parahaemolyticus and identifying it as a potential virulence factor.


FEMS Microbiol Lett


Lee CY,Cheng MF,Yu MS,Pan MJ





Has Abstract


2002-03-19 00:00:00














  • Fungal cytochrome P450s catalyzing hydroxylation of substituted toluenes to form their hydroxymethyl derivatives.

    abstract::The degradation of a series of nitroaromatic compounds by the lignin-degrading fungus Phanerochaete chrysosporium was examined. From 4-nitrotoluene (4-NT), several metabolic intermediates were identified. Initially, 4-NT was converted to 4-nitrobenzyl alcohol (4-NBA), followed by the oxidation reactions to form 4-nitr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Teramoto H,Tanaka H,Wariishi H

    更新日期:2004-05-15 00:00:00

  • Development and evaluation of a rapid, simple, sensitive, monoclonal antibody-based co-agglutination test for direct detection of Vibrio cholerae 01.

    abstract::Cholera epidemics caused by Vibrio cholerae 01 continue to represent a major public health concern in many developing countries. A rapid and simple test kit for the detection of V. cholerae 01 has been developed. The kit, CholeraScreen is a monoclonal antibody-based, co-agglutination test and is used directly with sto...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Colwell RR,Hasan JA,Huq A,Loomis L,Siebeling RJ,Torres M,Galvez S,Islam S,Tamplin MT,Bernstein D

    更新日期:1992-10-15 00:00:00

  • PCR performance of the highly thermostable proof-reading B-type DNA polymerase from Pyrococcus abyssi.

    abstract::DNA polymerase from the archaeon Pyrococcus abyssi strain Orsay was expressed in Escherichia coli. The recombinant DNA polymerase (Pab) was purified to homogeneity by heat treatment followed by 5 steps of chromatography and characterized for PCR applications. Buffer optimization experiments indicated that Pab PCR perf...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dietrich J,Schmitt P,Zieger M,Preve B,Rolland JL,Chaabihi H,Gueguen Y

    更新日期:2002-11-19 00:00:00

  • Construction and characterization of a Xanthomonas oryzae pv. oryzae bacterial artificial chromosome library.

    abstract::Xanthomonas oryzae pv. oryzae is an important plant pathogen which causes bacterial blight of rice. To facilitate genome studies of this bacterium, we have constructed a bacterial artificial chromosome (BAC) library of strain MAFF 311018. It consisted of 750 clones representing 16 genome equivalents, and had an insert...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ochiai H,Inoue Y,Hasebe A,Kaku H

    更新日期:2001-06-12 00:00:00

  • State of the art in eukaryotic nitrogenase engineering.

    abstract::Improving the ability of plants and plant-associated organisms to fix and assimilate atmospheric nitrogen has inspired plant biotechnologists for decades, not only to alleviate negative effects on nature from increased use and availability of reactive nitrogen, but also because of apparent economic benefits and opport...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Burén S,Rubio LM

    更新日期:2018-02-01 00:00:00

  • Differential regulation of physiological activities by RcsB and OmpR in Yersinia enterocolitica.

    abstract::A thorough understanding of the mechanisms of Rcs and EnvZ/OmpR phosphorelay systems that allow Yersinia enterocolitica to thrive in various environments is crucial to prevent and control Y. enterocolitica infections. In this study, we showed that RcsB and OmpR have the ability to function differently in modulating a ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Meng J,Bai J,Xu J,Huang C,Chen J

    更新日期:2019-09-01 00:00:00

  • Role of mycoferritin from Aspergillus parasiticus (255) in secondary metabolism (aflatoxin production).

    abstract::Aspergillus parasiticus (255), a non-toxigenic isolate showed the presence of secondary metabolites-aflatoxins (B1, B2, G1, G2) when grown in yeast extract sucrose media but not in basal media, thus demonstrating its toxigenic potential. Native PAGE of the crude protein isolated at different growth periods of A. paras...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shashidhar J,Sashidhar RB,Deshpande V

    更新日期:2005-10-01 00:00:00

  • The rfaH gene, which affects lipopolysaccharide synthesis in Salmonella enterica serovar Typhi, is differentially expressed during the bacterial growth phase.

    abstract::We have cloned and sequenced the rfaH gene from Salmonella enterica serovar Typhi strain Ty2. The gene showed a high degree of similarity to the rfaH genes from Escherichia coli K-12 and S. enterica serovar Typhimurium. A rfaH mutant was constructed by site-directed mutagenesis. This mutant produced a rough lipopolysa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Rojas G,Saldías S,Bittner M,Zaldívar M,Contreras I

    更新日期:2001-10-16 00:00:00

  • Isolation and characterization of Deinococcus geothermalis T27, a slightly thermophilic and organic solvent-tolerant bacterium able to survive in the presence of high concentrations of ethyl acetate.

    abstract::A solvent-tolerant, slightly thermophilic bacterium was isolated at 45 degrees C in the presence of toluene vapor provided as the sole carbon source. Strain T27 was identified as Deinococcus geothermalis T27. It could tolerate high concentrations of solvent provided as a nonaqueous layer (5% and 20%, v/v) to a cell su...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kongpol A,Kato J,Vangnai AS

    更新日期:2008-09-01 00:00:00

  • Lactobacillus reuteri A9 and Lactobacillus mucosae A13 isolated from Chinese superlongevity people modulate lipid metabolism in a hypercholesterolemia rat model.

    abstract::While there is strong evidence showing that many food-borne probiotics regulate cholesterol metabolism, few studies have examined how probiotics of human origin affect cholesterol metabolism. Because people living in so-called 'longevity villages' are unlikely to have hypercholesterolemia, we hypothesized that probiot...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jiang J,Feng N,Zhang C,Liu F,Zhao J,Zhang H,Zhai Q,Chen W

    更新日期:2019-12-01 00:00:00

  • General and kinetic properties of endoglucanase from Aspergillus niger.

    abstract::Endoglucanase of Aspergillus niger AS-101 was partially purified by ammonium sulphate fractionation and molecular sieving on Sephadex G-200. The enzyme was found to be unstable on storage, however, it received some protection on the addition of BSA, glycerol or sodium azide. Glycerol also protected the enzyme from ina...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Singh A,Agrawal AK,Abidi AB,Darmwal NS

    更新日期:1990-09-01 00:00:00

  • Attempts to revive Mycobacterium tuberculosis from 300-year-old human mummies.

    abstract::Environmental persistence of Mycobacterium tuberculosis is subject to speculation. However, the reality that infected postmortem tissues can be a danger to pathologists and embalmers has worrisome implications. A few experimental studies have demonstrated the organism's ability to withstand exposure to embalming fluid...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lemma E,Zimhony O,Greenblatt CL,Koltunov V,Zylber MI,Vernon K,Spigelman M

    更新日期:2008-06-01 00:00:00

  • Massive Open Online Courses: what will be their legacy?

    abstract::Massive Open Online Courses (MOOCs) dominated discussions of online learning and higher education in the news media and in universities between 2012 and 2015. However, fashions pass, needs change and technology evolves. This Commentary looks back, pauses on the present, and then looks forward. Whilst MOOCs are a signi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Daniel J

    更新日期:2016-04-01 00:00:00

  • Biotransformation of trinitrotoluene by Citrobacter sp. YC4 and evaluation of its cyto-toxicological effects.

    abstract::Trinitrotoluene (TNT) is an explosive chemical generally used for military, civil and industrial purposes. Therefore, TNT residue can be found in soil and ground water as an environmental pollutant. The environmental control of TNT pollution has become a critical issue because of its potential toxicity and carcinogeni...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kao CM,Wei SF,Chen SC,Yao CL,Ma C,Chien CC

    更新日期:2018-01-01 00:00:00

  • Giardia lamblia -- a model organism for eukaryotic cell differentiation.

    abstract::Giardia lamblia is a binucleated, flagellated protozoan parasite that inhabits the upper small intestine of its vertebrate hosts. The entire life cycle, which can be completed in vitro, is simple with cycling between a vegetative trophozoite and a highly resistant cystic form. The parasite is one of the earliest diver...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Svärd SG,Hagblom P,Palm JE

    更新日期:2003-01-21 00:00:00

  • Variant toxin B and a functional toxin A produced by Clostridium difficile C34.

    abstract::A particular property of Clostridium difficile strain C34 is an insertion of approximately 2 kb in the tcdA-C34 gene that does not hinder expression of a fully active TcdA-C34 molecule. Intoxication with TcdA-C34 induced an arborized appearance in eukaryotic cells (D-type cytopathic effect); intoxication with TcdB-C34...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mehlig M,Moos M,Braun V,Kalt B,Mahony DE,von Eichel-Streiber C

    更新日期:2001-05-01 00:00:00

  • Characterisation of the bovine enteric calici-like virus, Newbury agent 1.

    abstract::The bovine enteric calici-like virus, Newbury agent 1 (NA1) was characterised to determine if it is a member of the Caliciviridae and to establish its antigenic relationship to the established bovine enteric calicivirus Newbury agent 2 (NA2). Solid phase immune electron microscopy (SPIEM) allowed quantification of NA1...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dastjerdi AM,Snodgrass DR,Bridger JC

    更新日期:2000-11-01 00:00:00

  • Mini-Tn7 vectors as genetic tools for gene cloning at a single copy number in an industrially important and phytopathogenic bacteria, Xanthomonas spp.

    abstract::Transposon mini-Tn7 vectors insert into the chromosome of several Gram-negative bacteria in a site-specific manner. Here, we showed the application of mini-Tn7 as single copy site-specific integration vector system for Xanthomonas campestris pv. campestris. The transposition of the mini-Tn7 into the bacterial genome w...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jittawuttipoka T,Buranajitpakorn S,Fuangthong M,Schweizer HP,Vattanaviboon P,Mongkolsuk S

    更新日期:2009-09-01 00:00:00

  • Kinetics of electron transfer between plastocyanin and the soluble CuA domain of cyanobacterial cytochrome c oxidase.

    abstract::It has been shown that efficient functioning of photosynthesis and respiration in the cyanobacterium Synechocystis PCC 6803 requires the presence of either cytochrome c6 or plastocyanin. In order to check whether the blue copper protein plastocyanin can act as electron donor to cytochrome c oxidase, we investigated th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Paumann M,Bernroitner M,Lubura B,Peer M,Jakopitsch C,Furtmüller PG,Peschek GA,Obinger C

    更新日期:2004-10-15 00:00:00

  • Identification of the gene (aphA) encoding the class B acid phosphatase/phosphotransferase of Escherichia coli MG1655 and characterization of its product.

    abstract::An open reading frame located in the tyrB-uvrA intergenic region of the Escherichia coli MG1655 chromosome was identified as encoding the class B acid phosphatase of this species on the basis of cloning and expression experiments. A protocol for purification of the enzyme (named AphA) was developed, and its properties...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Thaller MC,Schippa S,Bonci A,Cresti S,Rossolini GM

    更新日期:1997-01-15 00:00:00

  • Catalase-peroxidase activity is decreased in a Caulobacter crescentus rho mutant.

    abstract::A Caulobacter crescentus rho::Tn5 mutant strain presenting a partially functional transcription termination factor Rho is highly sensitive to hydrogen peroxide in both exponential and stationary phases. The mutant was shown to be permanently under oxidative stress, based on fluorophore oxidation, and also to be sensit...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Italiani VC,Braz VS,Xiao H,Steinman HM,Marques MV

    更新日期:2010-02-01 00:00:00

  • Impact of oleic acid (cis-9-octadecenoic acid) on bacterial viability and biofilm production in Staphylococcus aureus.

    abstract::Staphylococcus aureus is responsible for a broad variety of chronic infections. Most S. aureus clinical isolates show the capacity to adhere to abiotic surfaces and to develop biofilms. Because S. aureus growing in a biofilm is highly refractory to treatment, inhibition of biofilm formation represents a major therapeu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Stenz L,François P,Fischer A,Huyghe A,Tangomo M,Hernandez D,Cassat J,Linder P,Schrenzel J

    更新日期:2008-10-01 00:00:00

  • Biochemical characterization of isovaleryl-CoA dehydrogenase (LiuA) of Pseudomonas aeruginosa and the importance of liu genes fora functional catabolic pathway of methyl-branched compounds.

    abstract::Growth of Pseudomonas aeruginosa on acyclic terpene alcohols (citronellol) and on other methyl-branched compounds such as leucine or isovalerate requires a functional leucine/isovalerate utilization (Liu) pathway. In this study, we investigated the liuABCDE gene cluster by insertion mutant analysis, heterologous expre...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Förster-Fromme K,Jendrossek D

    更新日期:2008-09-01 00:00:00

  • H4 histone in the macronucleus of Blepharisma japonicum (Protozoa, Ciliophora, Heterotrichida).

    abstract::Two clones, obtained by polymerase chain reaction from macronuclear DNA of the unicellular ciliated protist Blepharisma japonicum, were isolated and sequenced. They correspond to fragments of two different putative H4 histone genes. The existence of multiple H4 histone genes was also suggested by Southern blot hybridi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Salvini M,Bini E,Santucci A,Batistoni R

    更新日期:1997-04-01 00:00:00

  • Transcriptional repressor Rex is involved in regulation of oxidative stress response and biofilm formation by Streptococcus mutans.

    abstract::The transcriptional repressor Rex has been implicated in the regulation of energy metabolism and fermentative growth in response to redox potential. Streptococcus mutans, the primary causative agent of human dental caries, possesses a gene that encodes a protein with high similarity to members of the Rex family of pro...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Bitoun JP,Nguyen AH,Fan Y,Burne RA,Wen ZT

    更新日期:2011-07-01 00:00:00

  • Isolation of the dxr gene of Zymomonas mobilis and characterization of the 1-deoxy-D-xylulose 5-phosphate reductoisomerase.

    abstract::The gene encoding the second enzyme of the 2C-methyl-D-erythritol 4-phosphate (MEP) pathway for isopentenyl diphosphate biosynthesis, 1-deoxy-D-xylulose 5-phosphate (DXP) reductoisomerase, was cloned and sequenced from Zymomonas mobilis. The deduced amino acid sequence showed the highest identity (48.2%) to the DXP re...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Grolle S,Bringer-Meyer S,Sahm H

    更新日期:2000-10-01 00:00:00

  • Outer membrane proteins of gentamicin induced small colony variants of Pseudomonas aeruginosa.

    abstract::Small colony variants (SCVs) of Pseudomonas aeruginosa NCTC 6750 (WT) were repeatedly isolated in an in vitro kinetic model after exposure to gentamicin (GM). There were minor differences biochemically and in phage and serotyping between the wild type (WT) strain and SCVs. Changes in outer membrane protein profiles we...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Langford PR,Anwar H,Gonda I,Brown MR

    更新日期:1989-10-01 00:00:00

  • Conformational plasticity of molecular chaperones involved in periplasmic and outer membrane protein folding.

    abstract::How proteins reach their native conformation and location has been a major question of biology during the last 50 years. To counterbalance protein misfolding and the accumulation of aggregation products, a complex network of chaperones and proteases takes care of protein quality control in the cell. Such a chaperone n...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Mas G,Hiller S

    更新日期:2018-07-01 00:00:00

  • In vitro assay for the Bacillus subtilis signal peptidase SipS: systems for efficient in vitro transcription-translation and processing of precursors of secreted proteins.

    abstract::The signal peptidase (SPase) SipS of Bacillus subtilis is responsible for the processing of precursors of secreted proteins. It differs from the SPases of Gram-negative bacteria in structure and specificity. To assay the activity of SipS in vitro, two efficient transcription-translation systems for the synthesis of ra...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Vehmaanperä J,Görner A,Venema G,Bron S,van Dijl JM

    更新日期:1993-12-01 00:00:00

  • Biochemical characterization of alpha-amylase from the yeast Cryptococcus flavus.

    abstract::During our screening of amylolytic microorganisms from Brazilian fruits, we isolated a yeast strain classified as Cryptococcus flavus. When grown on starch-containing medium this strain exhibited the highest amylase production after 24 h of cultivation. The extracellular amylase from C. flavus was purified from the cu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wanderley KJ,Torres FA,Moraes LM,Ulhoa CJ

    更新日期:2004-02-16 00:00:00