Chromosome sizes and phylogenetic relationships between serotypes of Actinobacillus pleuropneumoniae.

Abstract:

:The genome size of Actinobacillus pleuropneumoniae was determined by pulsed field gel electrophoresis of AscI and ApaI digested chromosomal DNA. The genome size of the type strain 4074T (serotype 1) was determined to be 2404 +/- 40 kb. The chromosome sizes for the reference strains of the other serotypes range between 2.3 and 2.4 Mb. The restriction pattern profiles of AscI, ApaI and NheI digested chromosomes showed a high degree of polymorphism among the different serotype reference strains and allowed their discrimination. The analysis of the macrorestriction pattern polymorphism revealed phylogenetic relationships between the different serotype reference strains which reflect to some extent groups of serotypes known to cross-react serologically. In addition, different pulsed fields gel electrophoresis patterns also revealed heterogeneity in the chromosomal structure among different field strains of serotypes 1, 5a, and 5b, while strains of serotype 9 originating from most distant geographical places showed homogeneous ApaI patterns in pulsed field gel electrophoresis.

journal_name

FEMS Microbiol Lett

authors

Chevallier B,Dugourd D,Tarasiuk K,Harel J,Gottschalk M,Kobisch M,Frey J

doi

10.1111/j.1574-6968.1998.tb12913.x

subject

Has Abstract

pub_date

1998-03-15 00:00:00

pages

209-16

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(98)00033-0

journal_volume

160

pub_type

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