Abstract:
:Immunoassays are increasingly used to investigate the production, properties and fates of the cyanobacterial hepatotoxic microcystins in vitro and in vivo. Responses of an ELISA immunoassay to microcystins have been determined using the authentic toxin antigen, microcystin-LR, and conjugation products between the toxin and glutathione, cysteine-glycine and cysteine. The antibodies against microcystin-LR crossreacted with the toxin conjugation products with similar affinities (96-112%) to that of microcystin-LR, when assayed at a concentration of 1 microg l(-1). Toxicity assessment of the conjugates, in comparison to microcystin-LR, indicated a reduction according to mouse bioassay. In vitro protein phosphatase inhibition assay indicated that the conjugates possessed approximately 3-9-fold lower toxicity than microcystin-LR.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Metcalf JS,Beattie KA,Pflugmacher S,Codd GAdoi
10.1111/j.1574-6968.2000.tb09222.xkeywords:
subject
Has Abstractpub_date
2000-08-15 00:00:00pages
155-8issue
2eissn
0378-1097issn
1574-6968pii
S0378-1097(00)00270-6journal_volume
189pub_type
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