Immuno-crossreactivity and toxicity assessment of conjugation products of the cyanobacterial toxin, microcystin-LR.

Abstract:

:Immunoassays are increasingly used to investigate the production, properties and fates of the cyanobacterial hepatotoxic microcystins in vitro and in vivo. Responses of an ELISA immunoassay to microcystins have been determined using the authentic toxin antigen, microcystin-LR, and conjugation products between the toxin and glutathione, cysteine-glycine and cysteine. The antibodies against microcystin-LR crossreacted with the toxin conjugation products with similar affinities (96-112%) to that of microcystin-LR, when assayed at a concentration of 1 microg l(-1). Toxicity assessment of the conjugates, in comparison to microcystin-LR, indicated a reduction according to mouse bioassay. In vitro protein phosphatase inhibition assay indicated that the conjugates possessed approximately 3-9-fold lower toxicity than microcystin-LR.

journal_name

FEMS Microbiol Lett

authors

Metcalf JS,Beattie KA,Pflugmacher S,Codd GA

doi

10.1111/j.1574-6968.2000.tb09222.x

keywords:

subject

Has Abstract

pub_date

2000-08-15 00:00:00

pages

155-8

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(00)00270-6

journal_volume

189

pub_type

杂志文章
  • Outer membrane proteins of Bacteroides fragilis grown in vivo.

    abstract::The outer membrane protein (OMP) profiles of four different strains of Bacteroides fragilis, as determined by Coomassie blue stained polyacrylamide gels, were compared after growth in broth culture and in the mouse peritoneal cavity. There was no induction of the expression of large quantities of novel OMP after growt...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90022-i

    authors: Patrick S,Lutton DA

    更新日期:1990-09-01 00:00:00

  • A surface polysaccharide forms when gonococci are converted to serum resistance by cytidine 5'-monophospho-N-acetyl neuraminic acid.

    abstract::A serum-susceptible, guinea-pig chamber-passaged, laboratory strain (BS4 (agar)) of Neisseria gonorrhoeae was converted to serum resistance by incubation with cytidine 5-monophospho-N-acetyl neuraminic acid (CMP-NANA) and examined by electron microscopy after staining with ruthenium-red. In contrast to serum susceptib...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90261-n

    authors: Fox AJ,Curry A,Rowland PL,Lancaster S,Jones DM,Parsons NJ,Cole JA,Smith H

    更新日期:1990-01-01 00:00:00

  • Isolation of transposon mutants from Azospirillum brasilense Yu62 and characterization of genes involved in indole-3-acetic acid biosynthesis.

    abstract::The molecular genetics of indole-3-acetic (IAA) synthesis and regulation in Azospirillum brasilense was investigated in this study. Tn5 mutagenesis was performed and five mutants with decreased IAA production were isolated. Five Tn5-inserted genes from these mutants were cloned and sequenced. Four genes were reported ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.05.020

    authors: Xie B,Xu K,Zhao HX,Chen SF

    更新日期:2005-07-01 00:00:00

  • Detection of hepatitis B virus DNA by polymerase chain reaction in HBsAg negative Senegalese patients suffering from cirrhosis or primary liver cancer.

    abstract::The polymerase chain reaction was used to search for hepatitis B virus (HBV)-DNA sequences in the sera of HBsAg-negative Senegalese patients suffering from liver cirrhosis or liver cancer. Amplified HBV-DNA sequences were detected by hybridization with a digoxigenin-labelled HBV-DNA probe. HBV-DNA was detected in 17% ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(91)90439-h

    authors: Coursaget P,Le Cann P,Leboulleux D,Diop MT,Bao O,Coll AM

    更新日期:1991-09-15 00:00:00

  • Purification and properties of an esterase from Aspergillus nomius HS-1 degrading ethylene glycol dibenzoate.

    abstract::We isolated a fungal strain HS-1 utilizing either ethylene glycol dibenzoate or ethyl benzoate as sole source of carbon and energy, and identified it as Aspergillus nomius, based on morphological and rDNA analyses. An enzyme hydrolyzing the esters was purified from the culture supernatant of the strain to an electroph...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00353-7

    authors: Uchida H,Kurakata Y,Sawamura H,Inamura N,Kotani T,Uwajima T

    更新日期:2003-06-06 00:00:00

  • Genome diversity at the serA-linked capsule locus in Escherichia coli.

    abstract::Individual isolates of Escherichia coli synthesize one of more than 70 chemically distinct polysaccharides which form the capsule. In this article we review the genetics of capsule production in E. coli and highlight what this is beginning to reveal in terms of the genetic basis of the structural diversity of polysacc...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1992.tb14029.x

    authors: Boulnois G,Drake R,Pearce R,Roberts I

    更新日期:1992-12-15 00:00:00

  • The emergence of colistin-resistant Escherichia coli in chicken meats in Nepal.

    abstract::The emergence and dissemination of colistin resistance among Gram-negative bacteria is a global problem. We initiated a surveillance of colistin-resistant and -susceptible Escherichia coli in raw meats from chicken in Nepal. A total of 180 meat samples were collected; from these, 60 E. coli strains were isolated (33.3...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz237

    authors: Joshi PR,Thummeepak R,Leungtongkam U,Pooarlai R,Paudel S,Acharya M,Dhital S,Sitthisak S

    更新日期:2019-10-01 00:00:00

  • A novel phenol hydroxylase and catechol 2,3-dioxygenase from the thermophilic Bacillus thermoleovorans strain A2: nucleotide sequence and analysis of the genes.

    abstract::The new thermophilic Bacillus thermoleovorans strain A2 degrades phenol and cresols via the meta cleavage pathway. The first two enzymes involved in this process, the phenol hydroxylase and catechol 2,3-dioxygenase, encoded by the pheA and pheB genes respectively, were cloned and sequenced. The deduced amino acid sequ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb12926.x

    authors: Duffner FM,Müller R

    更新日期:1998-04-01 00:00:00

  • Association of attenuated mutants of Salmonella enterica serovar Enteritidis with porcine peripheral blood leukocytes.

    abstract::In this study, we were interested in the association of attenuated mutants of Salmonella enterica serovar Enteritidis with subpopulations of porcine white blood cells (WBC). The mutants included those with inactivated aroA, phoP, rfaL, rfaG, rfaC and fliC genes and a mutant with five major pathogenicity islands remove...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02305.x

    authors: Stepanova H,Volf J,Malcova M,Matiasovic J,Faldyna M,Rychlik I

    更新日期:2011-08-01 00:00:00

  • A novel phytase from Citrobacter gillenii: characterization and expression in Pichia pastoris (Komagataella pastoris).

    abstract::The phyCg gene encoding a new phytase from Citrobacter gillenii was optimized, synthesized, cloned and expressed in Pichia pastoris. Analysis of the amino acid sequence of the enzyme showed that it belongs to the histidine acid phosphatase family. The amino acid sequence of the PhyCg phytase has the highest homology (...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnaa217

    authors: Tkachenko AA,Kalinina AN,Borshchevskaya LN,Sineoky SP,Gordeeva TL

    更新日期:2020-12-21 00:00:00

  • Initiation of sporulation in Clostridium difficile: a twist on the classic model.

    abstract::The formation of dormant endospores is a complex morphological process that permits long-term survival in inhospitable environments for many Gram-positive bacteria. Sporulation for the anaerobic gastrointestinal pathogen Clostridium difficile is necessary for survival outside of the gastrointestinal tract of its host....

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/1574-6968.12499

    authors: Edwards AN,McBride SM

    更新日期:2014-09-01 00:00:00

  • Draft genome sequence of an aromatic compound-degrading bacterium, Desulfobacula sp. TS, belonging to the Deltaproteobacteria.

    abstract::Herein, we report a high-quality draft genome sequence of an uncultivated aromatic compound-degrading bacterium, obtained by the stable isotope probing method from a sulfate-reducing microcosm from an oil-contaminated tidal flat. The obtained genome was closely related with that of Desulfobacula toluolica Tol2. Abunda...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12591

    authors: Kim SJ,Park SJ,Jung MY,Kim JG,Min UG,Hong HJ,Rhee SK

    更新日期:2014-11-01 00:00:00

  • The F plasmid transfer activator TraJ is a dimeric helix-turn-helix DNA-binding protein.

    abstract::TraJ is an activator of the transfer (tra) operon in the F plasmid that counteracts H-NS silencing at the main transfer promoter (P(Y)). TraJ contains 226 aa (26 670 kDa), not 229 aa as reported previously, and forms homodimers. TraJ binds DNA containing P(Y)in vivo as demonstrated using a chromatin-immunoprecipitatio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.02064.x

    authors: Rodriguez-Maillard JM,Arutyunov D,Frost LS

    更新日期:2010-09-01 00:00:00

  • Chondroitinase C activity of Streptococcus intermedius.

    abstract::Chondroitin 6-sulfate depolymerizing activity was examined in the culture supernatant of Streptococcus intermedius ATCC 27335. 2-Acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-6-O-sulfo-D-galactose was split from the substrate. The enzyme(s) was not active upon chondroitin 4-sulfate or dermatan sulfate...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1989.tb03284.x

    authors: Hibi E,Osano E,Fujii Y,Moriyama T

    更新日期:1989-01-15 00:00:00

  • Functional characterization of the competence protein DprA/Smf in Escherichia coli.

    abstract::In several bacterial species that show natural transformation, dprA has been described as a competence gene. The DprA protein has been suggested to be involved in the protection of incoming DNA. However, members of the dprA gene family (also called smf) can be detected in virtually all bacterial species, which suggest...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00423.x

    authors: Smeets LC,Becker SC,Barcak GJ,Vandenbroucke-Grauls CM,Bitter W,Goosen N

    更新日期:2006-10-01 00:00:00

  • Environmental adaptation factors of two yeasts isolated from the leachate of a uranium mineral heap.

    abstract::Two yeasts from the effluent of a uranium mineral heap were identified as Candida sorbophila and Rhodotorula mucilaginosa. They are well adapted to such an environment as shown by their tolerance to metals and acid pH. However, different mechanisms could be involved in the adaptation: C. sorbophila could be considered...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11186.x

    authors: de Silóniz MI,Payo EM,Callejo MA,Marquina D,Peinado JM

    更新日期:2002-05-07 00:00:00

  • Regulatory systems controlling motility and gene transfer agent production and release in Rhodobacter capsulatus.

    abstract::Production of the gene transfer agent of Rhodobacter capsulatus, RcGTA, is dependent upon several cellular regulatory systems, including a putative phosphorelay involving the CtrA and CckA proteins. These proteins are also involved in flagellar motility in R. capsulatus. The interactions of proteins in this system are...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2012.02553.x

    authors: Mercer RG,Quinlan M,Rose AR,Noll S,Beatty JT,Lang AS

    更新日期:2012-06-01 00:00:00

  • Characterization of pRAS1-like plasmids from atypical North American psychrophilic Aeromonas salmonicida.

    abstract::Atypical psychrophilic Aeromonas salmonicida isolates were obtained from farmed and wild fish in Northeastern North America. These bacteria were isolated between 1992 and 2001 and carried tetracycline resistance (Tc(r)) plasmids of approximately 58 kb. The nine isolates had plasmids which could be divided into four gr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.10.039

    authors: Casas C,Anderson EC,Ojo KK,Keith I,Whelan D,Rainnie D,Roberts MC

    更新日期:2005-01-01 00:00:00

  • Role of oxidative stress and mitochondrial changes in cyanobacteria-induced apoptosis and hepatotoxicity.

    abstract::Microcystins produced by cyanobacteria are potent and specific hepatotoxins; however, the mechanisms of microcystin-induced hepatotoxicity have not been fully elucidated. The induction of free radical formation and mitochondrial alterations are two major events found in microcystin-treated cultured rat hepatocytes. Th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1016/S0378-1097(03)00100-9

    authors: Ding WX,Nam Ong C

    更新日期:2003-03-14 00:00:00

  • Gram-positive cell wall structure of the A3 gamma type in heliobacteria.

    abstract::The amino acid composition and structure of the peptidoglycan from Heliobacillus mobilis was determined by one- and two-dimensional thin-layer chromatography of completely and partially hydrolysed cell wall preparations. The structure was found to be of the A3 gamma type, with L,L-diaminopimelate in position 3, D-alan...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb07135.x

    authors: Pickett MW,Weiss N,Kelly DJ

    更新日期:1994-09-15 00:00:00

  • How did cyanobacteria first embark on the path to becoming plastids?: lessons from protist symbioses.

    abstract::Symbioses between phototrophs and heterotrophs (a.k.a 'photosymbioses') are extremely common, and range from loose and temporary associations to obligate and highly specialized forms. In the history of life, the most transformative was the 'primary endosymbiosis,' wherein a cyanobacterium was engulfed by a eukaryote a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fny209

    authors: Gavelis GS,Gile GH

    更新日期:2018-10-01 00:00:00

  • Plasmid-encoded gamma-hexachlorocyclohexane degradation genes and insertion sequences in Sphingobium francense (ex-Sphingomonas paucimobilis Sp+).

    abstract::The lin genes encode the gamma-hexachlorocyclohexane (gamma-HCH or lindane) catabolic pathway in lindane-degrading strains. The location and stability of these genes have been explored in the lindane-degrading Sphingobium francense strain Sp+, and in two non-lindane-degrading mutants (Sp1- and Sp2-). The lin genes, li...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00188.x

    authors: Cérémonie H,Boubakri H,Mavingui P,Simonet P,Vogel TM

    更新日期:2006-04-01 00:00:00

  • Quantification of disease progression of several microbial pathogens on Arabidopsis thaliana using real-time fluorescence PCR.

    abstract::An accurate monitoring of disease progression is important to evaluate disease susceptibility phenotypes. Over the years, Arabidopsis thaliana has become the model species to serve as a host in plant-pathogen interactions. Despite the efforts to study genetic mechanisms of host defense, little efforts are made for a t...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00759-6

    authors: Brouwer M,Lievens B,Van Hemelrijck W,Van den Ackerveken G,Cammue BP,Thomma BP

    更新日期:2003-11-21 00:00:00

  • Construction and properties of pyruvate dehydrogenase complexes with up to nine lipoyl domains per lipoate acetyltransferase chain.

    abstract::The lipoate acetyltransferase (E2p) subunits of the pyruvate dehydrogenase (PDH) complex of Escherichia coli have three tandemly repeated lipoyl domains, although net deletions of one or two has no apparent effect on the activity of the purified complexes. Plasmids containing IPTG-inducible aceEF-lpd operons, which en...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1992.tb14047.x

    authors: Machado RS,Clark DP,Guest JR

    更新日期:1992-12-15 00:00:00

  • The Giardia genome project database.

    abstract::The Giardia genome project database provides an online resource for Giardia lamblia (WB strain, clone C6) genome sequence information. The database includes edited single-pass reads, the results of BLASTX searches, and details of progress towards sequencing the entire 12 million-bp Giardia genome. Pre-sorted BLASTX re...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09242.x

    authors: McArthur AG,Morrison HG,Nixon JE,Passamaneck NQ,Kim U,Hinkle G,Crocker MK,Holder ME,Farr R,Reich CI,Olsen GE,Aley SB,Adam RD,Gillin FD,Sogin ML

    更新日期:2000-08-15 00:00:00

  • Epitopes of serogroup B Neisseria meningitidis analysed in vitro and directly from cerebrospinal fluid.

    abstract::Two type B15 P1.16 strains of Neisseria meningitidis were examined by immunogold electron microscopy for accessibility of two outer membrane protein (OMPs) to monoclonal antibodies. Both strains exhibited cell-to-cell variation of one epitope of the Class 3 OMP (P3.15) and one of the Class 1 OMPs (P1.16) when grown in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(89)90379-0

    authors: Wall RA,Davies HA,Borriello SP

    更新日期:1989-11-01 00:00:00

  • Hydroxyurea inhibits intracellular Toxoplasma gondii multiplication.

    abstract::Tachyzoites of Toxoplasma gondii multiply within the parasitophorous vacuole (PV) until the lysis of the host cell. This study was undertaken to evaluate the effect of hydroxyurea (a specific drug that arrests cell division at G1/S phase) on the multiplication of T. gondii tachyzoites in infected Vero cells. Infected ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/s0378-1097(00)00074-4

    authors: de Melo EJ,Mayerhoffer RO,de Souza W

    更新日期:2000-04-01 00:00:00

  • Interaction of dietary resveratrol with animal-associated bacteria.

    abstract::Resveratrol (3,5,4'-trihydroxy-trans-stilbene), an antifungal phytoalexin produced by grapes, peanuts, and Japanese knotweeds, is thought to be a beneficial dietary phytochemical in red wine and grape juice. Information on its antibacterial properties and biotransformation, however, is limited. We surveyed the interac...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01691.x

    authors: Jung CM,Heinze TM,Schnackenberg LK,Mullis LB,Elkins SA,Elkins CA,Steele RS,Sutherland JB

    更新日期:2009-08-01 00:00:00

  • Identification of the gene (aphA) encoding the class B acid phosphatase/phosphotransferase of Escherichia coli MG1655 and characterization of its product.

    abstract::An open reading frame located in the tyrB-uvrA intergenic region of the Escherichia coli MG1655 chromosome was identified as encoding the class B acid phosphatase of this species on the basis of cloning and expression experiments. A protocol for purification of the enzyme (named AphA) was developed, and its properties...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb10192.x

    authors: Thaller MC,Schippa S,Bonci A,Cresti S,Rossolini GM

    更新日期:1997-01-15 00:00:00

  • Direct profiling of the yeast dynamics in wine fermentations.

    abstract::We present a method to directly characterize the yeast diversity present in wine fermentations by employing denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 26S ribosomal RNA (rRNA) genes. PCR-DGGE of a portion of the 26S rRNA gene was shown to distinguish most yeast genera a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09210.x

    authors: Cocolin L,Bisson LF,Mills DA

    更新日期:2000-08-01 00:00:00