Purification and properties of an esterase from Aspergillus nomius HS-1 degrading ethylene glycol dibenzoate.

abstract：:The genus Xanthomonas is composed of several species that cause severe crop losses around the world. In Latin America, one of the most relevant species is Xanthomonas campestris pv. campestris, which is responsible for black rot in cruciferous plants. This pathogen causes yield losses in several cultures, including ca...

journal_title：FEMS microbiology letters

authors： Andrade AE,Silva LP,Pereira JL,Noronha EF,Reis FB Jr,Bloch C Jr,dos Santos MF,Domont GB,Franco OL,Mehta A

更新日期：2008-04-01 00:00:00

abstract：:The enterotoxigenic Escherichia coli (ETEC) strain Ec2173, causing post weaning diarrhoea in swine, harbours six plasmids ranging from 13 to 200 kb in size. The heat stable toxin genes sta, stb and a tetracycline resistance gene were located on a self conjugative 120-kb plasmid, called pTC. In the cloned ColE1 type or...

journal_title：FEMS microbiology letters

authors： Olasz F,Fekete PZ,Blum-Oehler G,Boldogkoi Z,Nagy B

更新日期：2005-03-15 00:00:00

abstract：:Piscirickettsia salmonis is the etiological agent of Salmonid Rickettsial Septicemia, a disease affecting salmon aquaculture industry. We analyzed the 16S-23S rDNA spacer region (internal transcribed spacer, ITS) of Chilean P. salmonis isolates LF-89 and EM-90. Two main ITS amplification products were obtained by PCR ...

journal_title：FEMS microbiology letters

authors： Casanova A,Obreque J,Sandino AM,Jashés M

更新日期：2001-04-01 00:00:00

abstract：:The phosphatidylcholine (PC) content of Aspergillus nidulans choC was varied by growing the auxotroph in medium containing various concentrations of choline chloride. Direct linear correlations were observed between PC content and in vivo chitin synthase activity, between in vivo chitin synthase activity and mean hyph...

journal_title：FEMS microbiology letters

authors： Binks PR,Robson GD,Goosey MW,Trinci AP

更新日期：1991-10-01 00:00:00

abstract：:Although very little, if any, beta-galactosidase activity is detected in Yersinia pestis by a standard Miller assay, we found that Y. pestis KIM6+ cells formed blue colonies on plates containing 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal). Searches of the Y. pestis genome databases revealed the presence of n...

journal_title：FEMS microbiology letters

authors： Bobrov AG,Perry RD

更新日期：2006-02-01 00:00:00

abstract：:The lin genes encode the gamma-hexachlorocyclohexane (gamma-HCH or lindane) catabolic pathway in lindane-degrading strains. The location and stability of these genes have been explored in the lindane-degrading Sphingobium francense strain Sp+, and in two non-lindane-degrading mutants (Sp1- and Sp2-). The lin genes, li...

journal_title：FEMS microbiology letters

authors： Cérémonie H,Boubakri H,Mavingui P,Simonet P,Vogel TM

更新日期：2006-04-01 00:00:00

abstract：:Fourteen strains representing 11 Caragana-nodulating Mesorhizobium genomic species were identified as representing Mesorhizobium amorphae, Mesorhizobium huakuii, Mesorhizobium septentrionale and groups related to Mesorhizobium plurifarium, Mesorhizobium temperatum, Mesorhizobium tianshanense and Mesorhizobium mediterr...

journal_title：FEMS microbiology letters

authors： Chen WF,Guan SH,Zhao CT,Yan XR,Man CX,Wang ET,Chen WX

更新日期：2008-06-01 00:00:00

abstract：:Aceticlastic methanogens metabolize acetate to methane and carbon dioxide. The central metabolism and the electron transport chains of these organisms have already been investigated. However, no particular attention has been paid to the mechanism by which acetate enters the archaeal cell. In our study we investigated ...

journal_title：FEMS microbiology letters

authors： Welte C,Kröninger L,Deppenmeier U

更新日期：2014-10-01 00:00:00

abstract：:Morphologically similar species such as Chinese black truffles and Tuber melanosporum were typed by restriction length polymorphism of internal transcribed spacers. This analysis together with sequence comparison revealed the presence of high genetic variability among fruit bodies collected in China. Selection of prim...

journal_title：FEMS microbiology letters

authors： Paolocci F,Rubini A,Granetti B,Arcioni S

更新日期：1997-08-15 00:00:00

abstract：:Digital game-based learning (DGBL) is being used increasingly as an alternative learning tool to teach science in further and higher education. A variety of digital game formats currently exist for science learning, alongside diverse methods for their implementation and evaluation. This paper aims to provide a broad s...

journal_title：FEMS microbiology letters

authors： Brown CL,Comunale MA,Wigdahl B,Urdaneta-Hartmann S

更新日期：2018-11-01 00:00:00

abstract：:Phytochromes are red/far-red light photoreceptors found in plants, cyanobacteria and heterotrophic bacteria. Biochemical analyses have established that the genes bphO and bphP (PA4116 and PA4117) of Pseudomonas aeruginosa encode both phytochrome components: BphO, a heme oxygenase that produces the linear tetrapyrrole ...

journal_title：FEMS microbiology letters

authors： Barkovits K,Harms A,Benkartek C,Smart JL,Frankenberg-Dinkel N

更新日期：2008-03-01 00:00:00

abstract：:Haemin (iron protoporphyrin IX) is an essential growth factor for the periodontal pathogen. Porphyromonas gingivalis. Iron protoporphyrin IX (IPP IX) binding to the avirulent P. gingivalis beige variant (W50/BE1) and the black-pigmenting parent wild-type strain W50 was quantified. W50/BE1 grown in a chemostat under ha...

journal_title：FEMS microbiology letters

authors： Smalley JW,Birss AJ,McKee AS,Marsh PD

更新日期：1996-07-15 00:00:00

abstract：:The binding and hemagglutinating activities of the B subunit(s) of the heat-labile enterotoxin (LTh-B) isolated from human enterotoxigenic Escherichia coli were investigated. The binding of 125I-labeled LTh-B to neuraminidase-treated human type B erythrocytes was most effectively inhibited by ganglioside GM1. A number...

journal_title：FEMS microbiology letters

authors： Sugii S,Tsuji T

更新日期：1990-01-01 00:00:00

abstract：:The ascomycete Sclerotinia sclerotiorum is a plant pathogen with a very broad host range. In order to identify and characterize genes involved in S. sclerotiorum infection of Brassica napus (canola), expressed sequence tags (ESTs) were examined from libraries prepared from three tissues: complex appressorium (infectio...

journal_title：FEMS microbiology letters

authors： Sexton AC,Cozijnsen AJ,Keniry A,Jewell E,Love CG,Batley J,Edwards D,Howlett BJ

更新日期：2006-05-01 00:00:00

abstract：:The flagellins of Methanospirillum hungatei strains JF1 and GP1, Methanococcus deltae, and Methanothermus fervidus are glycosylated. Isolated flagellar filaments from these organisms are dissociated by low concentrations (0.5% (v/v)) of Triton X-100. Flagellar filaments from other methanogens (Methanococcus voltae, Me...

journal_title：FEMS microbiology letters

authors： Faguy DM,Koval SF,Jarrell KF

更新日期：1992-01-01 00:00:00

abstract：:In Azotobacter chroococcum the hydrogenase structural genes (hupSL) cover about 2.8 kb of a 15-kb region associated with hydrogen-uptake (Hup) activity. Two other genes in this region, hupD and hupE, were located 8.9 kb downstream of hupL and were shown to be essential for hydrogenase activity by insertion mutagenesis...

journal_title：FEMS microbiology letters

authors： Du L,Stejskal F,Tibelius KH

更新日期：1992-09-01 00:00:00

abstract：:Bacteria are in constant conflict with competing bacterial and eukaryotic cells. To cope with the various challenges, bacteria developed distinct strategies, such as toxins that inhibit the growth or kill rivals of the same ecological niche. In recent years, two toxin systems have been discovered - the type VI secreti...

journal_title：FEMS microbiology letters

authors： Braun V,Patzer SI

更新日期：2013-08-01 00:00:00

abstract：:The regulation of nitrogen assimilation was investigated in the Gram-positive actinomycete Corynebacterium glutamicum. Biochemical studies and site-directed mutagenesis revealed that glutamine synthetase activity is regulated via adenylylation in this organism. The genes encoding the central signal transduction protei...

journal_title：FEMS microbiology letters

authors： Jakoby M,Krämer R,Burkovski A

更新日期：1999-04-15 00:00:00

abstract：:The gene encoding Aquifex aeolicus (Aae) DNA polymerase was expressed under the control of the trp promoter on a high-copy plasmid, pTRPNS, in Escherichia coli. The expressed enzyme was purified 11-fold with a 13.8% yield and a specific activity of 2268.3 U mg(-1). The optimum pH of the enzyme was 6.8-7.2. The optimal...

journal_title：FEMS microbiology letters

authors： Chang JR,Choi JJ,Kim HK,Kwon ST

更新日期：2001-07-10 00:00:00

abstract：:pSA1.1 is a 9.1-kb multicopy plasmid originally isolated from Streptomyces cyaneus (formerly S. azureus) ATCC 14921. This plasmid accumulates single-stranded DNA in S. lividans and is therefore considered to replicate by a rolling-circle replication. In the present work, the rep gene encoding the replication initiator...

journal_title：FEMS microbiology letters

authors： Yokoyama E,Matsuzaki Y,Doi K,Ogata S

更新日期：1998-12-01 00:00:00

abstract：:The pseudotrisaccharide allosamidin, a potent inhibitor of chitinases, retarded the fragmentation of hyphae but did not affect the fungal growth and cephalosporin-C production in Acremonium chrysogenum. In vitro inhibition of A. chrysogenum cell-bound chitinase(s) by allosamidin revealed that about 47% of the soluble ...

journal_title：FEMS microbiology letters

authors： Sándor E,Pusztahelyi T,Karaffa L,Karányi Z,Pócsi I,Biró S,Szentirmai A,Pócsi I

更新日期：1998-07-15 00:00:00

abstract：:Vibrio vulnificus, an opportunistic human pathogen, can obtain iron from a variety of heme proteins. This process involves the digestion of heme proteins by an exoprotease to liberate protoheme (iron-protoporphyrin IX). In the present study, we tested whether this pathogen also uses a synthetic heme compound, Fe-alpha...

journal_title：FEMS microbiology letters

authors： Miyoshi S,Kamei T,Inami Y,Ota Y,Yamamoto S,Tomochika K,Shinoda S

更新日期：1999-03-01 00:00:00

abstract：:DNA uptake in competent Streptococcus pneumoniae was strongly dependent on intracellular pH. Ionophore treatments that either acidified or alkalinized the cytoplasm reduced DNA transport. This indicates that the optimum pH for DNA uptake corresponds to the intracellular pH of competent bacteria which is 8.3 +/- 0.2. I...

journal_title：FEMS microbiology letters

authors： Clavé C,Trombe MC

更新日期：1989-11-01 00:00:00

abstract：:Alcaligenes sp. strain PPH degrades phenanthrene via 1-hydroxy-2-naphthoic acid (1-H2NA), 1,2-dihydroxynaphthalene (1,2-DHN), salicylic acid and catechol. Enzyme activity versus growth profile and heat stability studies suggested the presence of two distinct hydroxylases, namely 1-hydroxy-2-naphthoic acid hydroxylase ...

journal_title：FEMS microbiology letters

authors： Deveryshetty J,Phale PS

更新日期：2010-10-01 00:00:00

abstract：:A phylogenetic analysis based on 16S rRNA was performed on the genera Planococcus, Marinococcus, Sporosarcina and endospore-forming rods. In agreement with earlier 16S rRNA cataloguing data, Planococcus citreus and Sporosarcina ureae clustered with Bacillus pasteurii and other bacilli containing lysine in their cell w...

journal_title：FEMS microbiology letters

authors： Farrow JA,Ash C,Wallbanks S,Collins MD

更新日期：1992-06-01 00:00:00

abstract：:The production of candidate affinity proteins in a soluble form, for downstream characterization, is often a time-consuming step in combinatorial protein engineering methods. Here, a novel approach for efficient production of candidate clones is described based on direct cleavage of the affinity protein from the surfa...

journal_title：FEMS microbiology letters

authors： Kronqvist N,Löfblom J,Severa D,Ståhl S,Wernérus H

更新日期：2008-01-01 00:00:00

abstract：:The genus Pycnoporus forms a group of four species known especially for producing high redox potential laccases suitable for white biotechnology. A sample of 36 Pycnoporus strains originating from different geographical areas was studied to seek informative molecular markers for the typing of new strains in laboratory...

journal_title：FEMS microbiology letters

authors： Lesage-Meessen L,Haon M,Uzan E,Levasseur A,Piumi F,Navarro D,Taussac S,Favel A,Lomascolo A

更新日期：2011-12-01 00:00:00

abstract：:The constitutive expression vector pHY300-F1gfp was constructed to test the function of a promoter, F1, cloned from the rice epiphyte Bacillus brevis strain DX01. The DX01 cells harboring the plasmid pHY300-F1gfp were shown to produce bright green fluorescence. The results were confirmed by Western blot analysis and f...

journal_title：FEMS microbiology letters

authors： Chen Y,Yan J,Yang M,Wang J,Shen D

更新日期：2003-12-05 00:00:00

abstract：:Plasmodium falciparum sirtuin, PfSir2, contains histone deacetylase (HDAC) activity that may be central to the regulation of virulence gene expression in the parasites. Although a few reports have been published recently regarding in vitro and in vivo function of PfSir2, expression of the endogenous protein (c. 30 kDa...

journal_title：FEMS microbiology letters

authors： Prusty D,Mehra P,Srivastava S,Shivange AV,Gupta A,Roy N,Dhar SK

更新日期：2008-05-01 00:00:00

abstract：:Using pulsed-field gel electrophoresis (PFGE), nine profile types were identified in XbaI digests of a panel of strains of Salmonella enteritidis phage type 4 (PT 4) isolated in England and Wales between 1967 and 1992. When applied to 39 isolates from humans, chickens, poultry products and feed, strains belonging to p...

journal_title：FEMS microbiology letters

authors： Powell NG,Threlfall EJ,Chart H,Rowe B

更新日期：1994-06-01 00:00:00