Purification and properties of an esterase from Aspergillus nomius HS-1 degrading ethylene glycol dibenzoate.


:We isolated a fungal strain HS-1 utilizing either ethylene glycol dibenzoate or ethyl benzoate as sole source of carbon and energy, and identified it as Aspergillus nomius, based on morphological and rDNA analyses. An enzyme hydrolyzing the esters was purified from the culture supernatant of the strain to an electrophoretically homogeneous state. The enzyme was a carboxyl esterase with a monomeric structure, of which the molecular mass was about 60000, and inhibited by phenylmethylsulfonyl fluoride. The enzyme hydrolyzed various benzoate esters and p-nitrophenyl esters, and its hydrolysis rates for the most favorable substrates, n-butyl benzoate and p-nitrophenyl valerate, were comparable.


FEMS Microbiol Lett


Uchida H,Kurakata Y,Sawamura H,Inamura N,Kotani T,Uwajima T





Has Abstract


2003-06-06 00:00:00














  • Mutational, proteomic and metabolomic analysis of a plant growth promoting copper-resistant Pseudomonas spp.

    abstract::Pseudomonas sp. TLC6-6.5-4 is a multiple metal resistant plant growth-promoting bacteria isolated from copper-contaminated lake sediments. In this study, a comprehensive analysis of genes involved in copper resistance was performed by generating a library of transposon (Tn5) mutants. Two copper-sensitive mutants with ...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Li K,Pidatala RR,Ramakrishna W

    更新日期:2012-10-01 00:00:00

  • The ligase chain reaction distinguishes hepatitis B virus S-gene variants.

    abstract::A novel method for the identification of hepatitis B virus (HBV) variants was developed. The ligase chain reaction (LCR) distinguished the sequences of two isolates from the Gambia showing a change at nucleotide 421 within the region coding for the surface protein a antigenic determinant. One sequence was derived from...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Karthigesu VD,Mendy M,Fortuin M,Whittle HC,Howard CR,Allison LM

    更新日期:1995-09-01 00:00:00

  • Fungal cytochrome P450s catalyzing hydroxylation of substituted toluenes to form their hydroxymethyl derivatives.

    abstract::The degradation of a series of nitroaromatic compounds by the lignin-degrading fungus Phanerochaete chrysosporium was examined. From 4-nitrotoluene (4-NT), several metabolic intermediates were identified. Initially, 4-NT was converted to 4-nitrobenzyl alcohol (4-NBA), followed by the oxidation reactions to form 4-nitr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Teramoto H,Tanaka H,Wariishi H

    更新日期:2004-05-15 00:00:00

  • Isolation of the fenoxaprop-ethyl (FE)-degrading bacterium Rhodococcus sp. T1, and cloning of FE hydrolase gene feh.

    abstract::An enrichment culture which completely degraded fenoxaprop-ethyl (FE) was acquired by using FE as sole carbon source. An efficient FE-degrading strain T1 was isolated from the enrichment culture and identified as Rhodococcus sp. Strain T1 could degrade 94% of 100 mg L(-1) FE within 24 h and the metabolite fenoxaprop a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hou Y,Tao J,Shen W,Liu J,Li J,Li Y,Cao H,Cui Z

    更新日期:2011-10-01 00:00:00

  • Listeria monocytogenes ferritin protects against multiple stresses and is required for virulence.

    abstract::In this study, the role of Listeria monocytogenes ferritin was investigated. The fri gene encoding the ferritin was deleted and the phenotype of the mutant was analyzed demonstrating that ferritin is necessary for optimal growth in minimal medium in both presence and absence of iron, as well as after cold- and heat-sh...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dussurget O,Dumas E,Archambaud C,Chafsey I,Chambon C,Hébraud M,Cossart P

    更新日期:2005-09-15 00:00:00

  • Characterization of a periplasmic peptidyl-prolyl cis-trans isomerase in Erwinia chrysanthemi.

    abstract::The main determinant of the plant pathogen Erwinia chrysanthemi virulence is the production of extracellular enzymes, mainly pectate lyases. Adjacent to a pectate lyase encoding locus, we identified the gene rotA supposed to encode a folding catalyst. Overproduction of the protein and assay of activity using a synthet...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Pissavin C,Hugouvieux-Cotte-Pattat N

    更新日期:1997-12-01 00:00:00

  • Evaluating efficacy of bacteriophage therapy against Staphylococcus aureus infections using a silkworm larval infection model.

    abstract::Silkworm larva has recently been recognized as an alternative model animal for higher mammals to evaluate the effects of antibiotics. In this study, we examined the efficacy of the bacteriophage (phage) therapy, which harnesses phages as antibacterial agents, against Staphylococcus aureus infections, using the silkwor...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Takemura-Uchiyama I,Uchiyama J,Kato S,Inoue T,Ujihara T,Ohara N,Daibata M,Matsuzaki S

    更新日期:2013-10-01 00:00:00

  • The Pseudomonas aeruginosa RhlR-controlled aegerolysin RahU is a low-affinity rhamnolipid-binding protein.

    abstract::The opportunistic pathogen Pseudomonas aeruginosa uses quorum-sensing systems to regulate collective behaviour in response to the environment, by linking the expression of particular genes to population density. The quorum-sensing transcription factors LasR and RhlR and their cognate N-acyl-homoserine lactone (HSL) si...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Miklavič Š,Kogovšek P,Hodnik V,Korošec J,Kladnik A,Anderluh G,Gutierrez-Aguirre I,Maček P,Butala M

    更新日期:2015-05-01 00:00:00

  • High-quality mutant libraries of Xanthomonas oryzae pv. oryzae and X. campestris pv. campestris generated by an efficient transposon mutagenesis system.

    abstract::A novel transposon mutagenesis system for the phytopathogenic bacteria Xanthomonas oryzae pv. oryzae (Xoo) and X. campestris pv. campestris (Xcc) was developed using a Tn5-based transposome. A highly efficient transformation up to 10(6) transformants per microg transposon DNA was obtained. Southern blot and thermal as...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sun Q,Wu W,Qian W,Hu J,Fang R,He C

    更新日期:2003-09-12 00:00:00

  • bkaR is a TetR-type repressor that controls an operon associated with branched-chain keto-acid metabolism in Mycobacteria.

    abstract::This study describes how bkaR, a highly conserved mycobacterial TetR-like transcriptional repressor, regulates a number of nearby genes that have associations with branched-chain keto-acid metabolism. bkaR (MSMEG_4718) was deleted from the nonpathogenic species Mycobacterium smegmatis, and changes in global gene expre...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Balhana RJ,Swanston SN,Coade S,Withers M,Sikder MH,Stoker NG,Kendall SL

    更新日期:2013-08-01 00:00:00

  • Isolation and identification of nematode-antagonistic compounds from the fungus Aspergillus candidus.

    abstract::Culture medium from an isolate of the fungus Aspergillus candidus was extracted, fractionated and examined to discover compounds antagonistic to plant-parasitic nematodes that are important pathogens of agricultural crops. Column, thin layer and preparative chromatographies and spectral and elemental analyses, were us...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shemshura ON,Bekmakhanova NE,Mazunina MN,Meyer SL,Rice CP,Masler EP

    更新日期:2016-03-01 00:00:00

  • A molecular marker diagnostic of a specific isolate of an arbuscular mycorrhizal fungus, Gigaspora margarita.

    abstract::To investigate the auto-ecology of a strain of Gigaspora margarita in a commercial inoculum, we found a pair of PCR primers amplifying a sequence of 235 bp diagnostic of the isolate. We designed an oligonucleotide probe based on the DNA sequence. The combination of PCR and the probing successfully detected the diagnos...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Yokoyama K,Tateishi T,Marumoto T,Saito M

    更新日期:2002-07-02 00:00:00

  • The mode of action of the plant antimicrobial peptide MiAMP1 differs from that of its structural homologue, the yeast killer toxin WmKT.

    abstract::The plant antimicrobial peptide MiAMP1 from Macadamia integrifolia and the yeast killer toxin peptide WmKT from Williopsis mrakii are structural homologues. Comparative studies of yeast mutants were performed to test their sensitivity to these two antimicrobial peptides. No differences in susceptibility to MiAMP1 were...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Stephens C,Kazan K,Goulter KC,Maclean DJ,Manners JM

    更新日期:2005-02-01 00:00:00

  • Laboratory-acquired lethal infections by potential bioweapons pathogens including Ebola in 2014.

    abstract::Researchers studying both cellular and viral disease agents in the laboratory have become infected since the early days of microbiology 150 years ago. However, in the early 21st century, new concerns about bioweapons being used to generate terror and also with a series of newly emerging or newly understood disease-cau...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Silver S

    更新日期:2015-01-01 00:00:00

  • Structure and function of a cold shock domain fold protein, CspD, in Janthinobacterium sp. Ant5-2 from East Antarctica.

    abstract::A cold shock domain (CSD)-containing protein, CspD, of molecular mass ~7.28 kDa in a psychrotolerant Antarctic Janthinobacterium sp. Ant5-2 (ATCC BAA-2154) exhibited constitutive expression at 37, 22, 15, 4 and -1°C. The cspD gene encoding the CspD protein of Ant5-2 was cloned, sequenced and analyzed. The deduced prot...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mojib N,Andersen DT,Bej AK

    更新日期:2011-06-01 00:00:00

  • Differentiation of enterococci from other group D streptococci by means of a specific monoclonal antibody.

    abstract::We report on the specificity of a monoclonal antibody which reacts with autoclaved extracts of four species of enterococci but does not react to the same extent with similar extracts from two non-enterococcal group D streptococci. The monoclonal antibody also reacts specifically with purified lipoteichoic acid from St...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hammonds SJ,Easterbrook TJ,McIllmurray MB

    更新日期:1991-07-15 00:00:00

  • Efficient cloning in Bacillus megaterium: comparison to Bacillus subtilis and Escherichia coli cloning hosts.

    abstract::Quantitative cloning efficiencies for B. megaterium, B. subtilis, and E. coli were compared. Transformation of B. megaterium is less efficient than transformation of B. subtilis or E. coli. The frequency of recombinant clones was equal in E. coli and B. megaterium; both somewhat higher than in B. subtilis. Equivalent ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Von Tersch MA,Robbins HL

    更新日期:1990-08-01 00:00:00

  • Dr fimbriae coding region associated hemolytic activity of Escherichia coli.

    abstract::We investigated the hemolytic activity of Escherichia coli strain EC901 carrying plasmid pBJN406 containing genes draA-E involved in expression of the mannose-resistant Dr hemagglutinin, and in its isogenic insertion mutants devised with Tn5, Tn3, and TnphoA. While E. coli BN406 displayed rapid hemolytic activity agai...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Goluszko P,Nowicki S,Kaul AK,Pham T,Nowicki BJ

    更新日期:1995-07-15 00:00:00

  • Efficient extracellular production of hybrid E. coli heat-labile enterotoxin B subunits in a marine Vibrio.

    abstract::Escherichia coli heat-labile enterotoxin B subunit (EtxB) has been proposed as a potential protein carrier for the delivery of heterologous peptides to target cells, particularly for the oral delivery of epitopes to the mucosal immune system. In this study, two extensions to the C-terminus of EtxB were genetically eng...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Marcello A,Loregian A,Palù G,Hirst TR

    更新日期:1994-03-15 00:00:00

  • Mobilizable narrow host range plasmids as natural suicide vectors enabling horizontal gene transfer among distantly related bacterial species.

    abstract::Klebsiella pneumoniae 287-w carries three small narrow host range (NHR) plasmids (pIGMS31, pIGMS32, and pIGRK), which could be maintained in several closely related species of Gammaproteobacteria, but not in Alphaproteobacteria. The plasmids contain different mobilization systems (MOB), whose activity in Escherichia c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Smorawinska M,Szuplewska M,Zaleski P,Wawrzyniak P,Maj A,Plucienniczak A,Bartosik D

    更新日期:2012-01-01 00:00:00

  • Characterization of pRAS1-like plasmids from atypical North American psychrophilic Aeromonas salmonicida.

    abstract::Atypical psychrophilic Aeromonas salmonicida isolates were obtained from farmed and wild fish in Northeastern North America. These bacteria were isolated between 1992 and 2001 and carried tetracycline resistance (Tc(r)) plasmids of approximately 58 kb. The nine isolates had plasmids which could be divided into four gr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Casas C,Anderson EC,Ojo KK,Keith I,Whelan D,Rainnie D,Roberts MC

    更新日期:2005-01-01 00:00:00

  • RD1 region in mycobacterial genome is involved in the induction of necrosis in infected RAW264 cells via mitochondrial membrane damage and ATP depletion.

    abstract::It was shown that virulent Mycobacterium tuberculosis H37Rv induces necrosis of infected RAW264 cells at 24 h post infection while avirulent H37Ra and an attenuated H37Rv mutant that is deficient for RD1 region (H37RvDeltaRD1) cause less necrosis of the infected cells. While H37Rv caused damage of the mitochondrial in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kaku T,Kawamura I,Uchiyama R,Kurenuma T,Mitsuyama M

    更新日期:2007-09-01 00:00:00

  • Serine racemase homologue of Saccharomyces cerevisiae has L-threo-3-hydroxyaspartate dehydratase activity.

    abstract::The NH(2)-terminal amino acid sequence of L-threo-3-hydroxyaspartate dehydratase from Pseudomonas sp. T62 showed significant similarity to that of the SRY1 gene product of Saccharomyces cerevisiae (serine racemase in yeast). SRY1 was cloned and expressed in Escherichia coli, and the gene product was purified and parti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wada M,Nakamori S,Takagi H

    更新日期:2003-08-29 00:00:00

  • Phospholipid etherlipid and phospholipid fatty acid fingerprints in selected euryarchaeotal monocultures for taxonomic profiling.

    abstract::Phospholipid etherlipid (PLEL) derived isoprenoids and phospholipid fatty acids (PLFA) were determined in eight Euryarchaeotal monocultures for taxonomic profiling. For the first time significant amounts of fatty acids in the PLFA of Euryarchaeota were determined. The PLFA proportion varied between 11.3 and 35.5% of t...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gattinger A,Schloter M,Munch JC

    更新日期:2002-07-16 00:00:00

  • A surface polysaccharide forms when gonococci are converted to serum resistance by cytidine 5'-monophospho-N-acetyl neuraminic acid.

    abstract::A serum-susceptible, guinea-pig chamber-passaged, laboratory strain (BS4 (agar)) of Neisseria gonorrhoeae was converted to serum resistance by incubation with cytidine 5-monophospho-N-acetyl neuraminic acid (CMP-NANA) and examined by electron microscopy after staining with ruthenium-red. In contrast to serum susceptib...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fox AJ,Curry A,Rowland PL,Lancaster S,Jones DM,Parsons NJ,Cole JA,Smith H

    更新日期:1990-01-01 00:00:00

  • The NarE protein of Neisseria gonorrhoeae catalyzes ADP-ribosylation of several ADP-ribose acceptors despite an N-terminal deletion.

    abstract::The ADP-ribosylating enzymes are encoded in many pathogenic bacteria in order to affect essential functions of the host. In this study, we show that Neisseria gonorrhoeae possess a locus that corresponds to the ADP-ribosyltransferase NarE, a previously characterized enzyme in N. meningitidis The 291 bp coding sequence...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Rodas PI,Álamos-Musre AS,Álvarez FP,Escobar A,Tapia CV,Osorio E,Otero C,Calderón IL,Fuentes JA,Gil F,Paredes-Sabja D,Christodoulides M

    更新日期:2016-09-01 00:00:00

  • Use of a microarray to assess the distribution of plasmid and chromosomal virulence genes in strains of enteroaggregative Escherichia coli.

    abstract::A DNA microarray was used to analyze the distribution of plasmid and chromosomal genes among strains of enteroaggregative Escherichia coli (EAEC) isolated from a prospective diarrhoea surveillance study in the United Kingdom. Target genes were extracted from existing databases and from the genome sequence of prototype...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jenkins C,van Ijperen C,Dudley EG,Chart H,Willshaw GA,Cheasty T,Smith HR,Nataro JP

    更新日期:2005-12-01 00:00:00

  • Efficient screening for astaxanthin-overproducing mutants of the yeast Xanthophyllomyces dendrorhous by flow cytometry.

    abstract::Astaxanthin possesses higher antioxidant activity than other carotenoids and, for this and other reasons, has great commercial potential for use in the aquaculture, pharmaceutical, and food industries. The basidiomycetous yeast Xanthophyllomyces dendrorhous is one of the best natural producers of astaxanthin, but wild...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ukibe K,Katsuragi T,Tani Y,Takagi H

    更新日期:2008-09-01 00:00:00

  • Myo-inositol facilitators IolT1 and IolT2 enhance D-mannitol formation from D-fructose in Corynebacterium glutamicum.

    abstract::Reduction of D-fructose to D-mannitol by whole-cell biotransformation with recombinant resting cells of Corynebacterium glutamicum ATCC13032 requires the coexpression of mdh and fdh, which encode mannitol and formate dehydrogenases, respectively. However, d-mannitol formation is limited by the uptake of d-fructose in ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Bäumchen C,Krings E,Bringer S,Eggeling L,Sahm H

    更新日期:2009-01-01 00:00:00

  • Alteration in the composition of cholesteryl glucosides and other lipids in Helicobacter pylori undergoing morphological change from spiral to coccoid form.

    abstract::In this analysis of membrane lipid compositions in Helicobacter pylori, the membrane lipid profiles drastically changed during coccoid formation: cholesteryl-6-O-tetradecanoyl-alpha-D-glucopyranoside levels increased, cholesteryl-alpha-D-glucopyranoside and phosphatidyl ethanolamine decreased, and a coordinated increa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shimomura H,Hayashi S,Yokota K,Oguma K,Hirai Y

    更新日期:2004-08-15 00:00:00