Identification of a glycolytic regulon in the archaea Pyrococcus and Thermococcus.

abstract：:Both form I and II ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) genes were detected in Thiobacillus intermedius by heterologous hybridization using specific probes from Anacystis nidulans and Rhodobacter sphaeroides, respectively. However, only the previously reported form I enzyme could be demonstrated i...

journal_title：FEMS microbiology letters

authors： Stoner MT,Shively JM

更新日期：1993-03-01 00:00:00

abstract：:Salmonella enterica serovar Enteritidis (S. Enteritidis) possesses plasmids of different sizes and roles. Besides the serovar-specific virulence plasmid present in most field strains, S. Enteritidis can harbour plasmids of low molecular mass whose biological role is poorly understood. We therefore sequenced plasmid pC...

journal_title：FEMS microbiology letters

authors： Gregorova D,Pravcova M,Karpiskova R,Rychlik I

更新日期：2002-09-10 00:00:00

abstract：:This report describes Vibrio seventh pandemic island II (VSP-II) and three novel variants revealed by comparative genomics of 23 Vibrio cholerae strains and their presence among a large and diverse collection of V. cholerae isolates. Three VSP-II variants were reported previously and our results demonstrate the presen...

journal_title：FEMS microbiology letters

authors： Taviani E,Grim CJ,Choi J,Chun J,Haley B,Hasan NA,Huq A,Colwell RR

更新日期：2010-07-01 00:00:00

abstract：:The identification of virulence factors in Rhodococcus equi has been severely hampered by the lack of a method for in vivo random insertion mutagenesis. This study reports the use of transposomes to generate random insertions of a gene conferring kanamycin resistance into the genome of R. equi ATCC 33701. Southern hyb...

journal_title：FEMS microbiology letters

authors： Mangan MW,Meijer WG

更新日期：2001-12-18 00:00:00

abstract：:Growth of a Mycobacterium avium complex serotype 20 rough-colony variant in 1.0% Tween 80 resulted in a smooth-colony morphological conversion that was reversible upon removal of Tween and was not associated with the presence of serotype-specific glycopeptidolipid antigens. Electron microscopic examination suggested t...

journal_title：FEMS microbiology letters

authors： Wright EL,Pourshafie M,Barrow WW

更新日期：1992-11-01 00:00:00

abstract：:Previously-developed PCR protocols specific for the 16S rRNA gene of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli were adapted for the detection of these species in human faeces, following DNA extraction and purification using mini-prep columns. The limits of detection in seeded faeces f...

journal_title：FEMS microbiology letters

authors： Mikosza AS,La T,Margawani KR,Brooke CJ,Hampson DJ

更新日期：2001-04-13 00:00:00

abstract：:A high-level expression system for the Escherichia coli HB101 gamma-glutamyltranspeptidase (GGT) gene was constructed to obtain a sufficient amount of the enzyme for structural studies. About 300 mg of the enzyme was purified from 2 1 of culture broth. Both subunits were needed to reconstitute GGT activity. The E. col...

journal_title：FEMS microbiology letters

authors： Ishiye M,Niwa M

更新日期：1992-10-15 00:00:00

abstract：:Mild alkaline solutions (beta-elimination), after removing the non-covalently bonded wall materials by hot SDS, released 13% and 26% of remaining wall proteins from mycelial and yeast cells of Candida albicans, respectively. When the beta-elimination was carried out after digestion of the walls with chitinase, four-fo...

journal_title：FEMS microbiology letters

authors： Mormeneo S,Marcilla A,Iranzo M,Sentandreu R

更新日期：1994-10-15 00:00:00

abstract：:Multidrug efflux pumps contribute to multiple antibiotic resistance in Pseudomonas aeruginosa. Pump expression usually has been quantified by Western blotting. Quantitative real-time polymerase chain reaction has been developed to measure mRNA expression for genes of interest. Whether this method correlates with pump ...

journal_title：FEMS microbiology letters

authors： Yoneda K,Chikumi H,Murata T,Gotoh N,Yamamoto H,Fujiwara H,Nishino T,Shimizu E

更新日期：2005-02-01 00:00:00

abstract：:The aims of our research were to investigate the gene expression of the multidrug efflux transporter, CDR1 and the major drug facilitator superfamily transporter, MDR1 gene in azole drug-resistant Candida albicans and Candida glabrata clinical isolates recovered from vaginitis patients; and to identify hotspot mutatio...

journal_title：FEMS microbiology letters

authors： Looi CY,D' Silva EC,Seow HF,Rosli R,Ng KP,Chong PP

更新日期：2005-08-15 00:00:00

abstract：:The bovine enteric calici-like virus, Newbury agent 1 (NA1) was characterised to determine if it is a member of the Caliciviridae and to establish its antigenic relationship to the established bovine enteric calicivirus Newbury agent 2 (NA2). Solid phase immune electron microscopy (SPIEM) allowed quantification of NA1...

journal_title：FEMS microbiology letters

authors： Dastjerdi AM,Snodgrass DR,Bridger JC

更新日期：2000-11-01 00:00:00

abstract：:One hundred and ninety-eight clinical isolates of Mycobacterium kansasii collected between 2003 and 2004 in Japan were genotyped by PCR and restriction enzyme analysis (PRA) and 16S-23S internal transcribed spacer (ITS) sequencing. The results demonstrated that clinical isolates of M. kansasii in Japan are almost excl...

journal_title：FEMS microbiology letters

authors： Iwamoto T,Saito H

更新日期：2006-01-01 00:00:00

abstract：:A screening for hydrogen uptake (hup) genes in Rhizobium leguminosarum bv. viciae isolates from different locations within Spain identified no Hup+ strains, confirming the scarcity of the Hup trait in R. leguminosarum. However, five new Hup+ strains were isolated from Ni-rich soils from Italy and Germany. The hup gene...

journal_title：FEMS microbiology letters

authors： Fernández D,Toffanin A,Palacios JM,Ruiz-Argüeso T,Imperial J

更新日期：2005-12-01 00:00:00

abstract：:Ninety-three Salmonella isolates recovered from commercial foods and exotic animals in Colombia were studied. The serotypes, resistance profiles and where applicable the quinolone resistance genes were determined. Salmonella Anatum (n=14), Uganda (19), Braenderup (10) and Newport (10) were the most prevalent serovars,...

journal_title：FEMS microbiology letters

authors： Karczmarczyk M,Martins M,McCusker M,Mattar S,Amaral L,Leonard N,Aarestrup FM,Fanning S

更新日期：2010-12-01 00:00:00

abstract：:The DnaK chaperone system is involved in various cellular processes such as the control of the folded and oligomeric state of proteins under stress and non-stress conditions. In this study we functionally characterised the homologues of the DnaK system from Clostridium acetobutylicum DnaK, DnaJ, GrpE and OrfA were het...

journal_title：FEMS microbiology letters

authors： Rüngeling E,Laufen T,Bahl H

更新日期：1999-01-01 00:00:00

abstract：:The flaA gene, which codes for the Spirochaeta aurantia flagellar filament outer layer polypeptide, FlaA, was cloned, sequenced and analysed. The gene appears to be transcribed into a monocistronic mRNA from a sigma 70-like promoter. The translational start is 31 base pairs after the start of the transcript, the open ...

journal_title：FEMS microbiology letters

authors： Parales J Jr,Greenberg EP

更新日期：1993-02-01 00:00:00

abstract：:Two isolates, belonging to a new species of a novel genus of the Phylum "Deinococcus/Thermus ", were recovered from hot spring runoffs on the Island of São Miguel in the Azores. Strains RQ-24(T) and TU-8 are the first cultured representatives of a distinct phylogenetic lineage within this phylum. These strains form or...

journal_title：FEMS microbiology letters

authors： Albuquerque L,Simões C,Nobre MF,Pino NM,Battista JR,Silva MT,Rainey FA,da Costa MS

更新日期：2005-06-15 00:00:00

abstract：:Immunoassays are increasingly used to investigate the production, properties and fates of the cyanobacterial hepatotoxic microcystins in vitro and in vivo. Responses of an ELISA immunoassay to microcystins have been determined using the authentic toxin antigen, microcystin-LR, and conjugation products between the toxi...

journal_title：FEMS microbiology letters

authors： Metcalf JS,Beattie KA,Pflugmacher S,Codd GA

更新日期：2000-08-15 00:00:00

abstract：:The SbmA protein is involved in the transport of MccB17-, MccJ25-, bleomycin- and proline-rich peptides into the Escherichia coli cytoplasm. sbmA gene homologues were found in a variety of bacteria. However, the physiological role of this protein still remains unknown. Previously, we found that a combination of sbmA a...

journal_title：FEMS microbiology letters

authors： Corbalán NS,Adler C,de Cristóbal RE,Pomares MF,Delgado MA,Vincent PA

更新日期：2010-10-01 00:00:00

abstract：:Contrary to its effect on rich medium, D-cycloserine showed no bactericidal effect on Zymomonas mobilis cells cultured on mineral medium. Addition of a mixture of glycine and glutamic acid to the mineral medium restored its bactericidal action. However, mutant enrichments run in these conditions were biased, with most...

journal_title：FEMS microbiology letters

authors： Pencreac'h G,Aït-Abdelkader N,Joset F,Baratti JC

更新日期：1996-09-01 00:00:00

abstract：:Computational comparative techniques were applied to analysis of the aromatic amino acid regulon in Gram-positive bacteria. A new candidate transcription regulation signal of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase and shikimate kinase genes was identified in Streptococcus and Lactococcus species. New T-b...

journal_title：FEMS microbiology letters

authors： Panina EM,Vitreschak AG,Mironov AA,Gelfand MS

更新日期：2003-05-28 00:00:00

abstract：:Mapping of the plasmid-encoded RNA of the intracellular parasite, Chlamydia trachomatis revealed that the upstream control elements are different from those of other Gram-negative bacteria. A tetranucleotide, AYAA was found near the -10 position, in 5 out of 8 upstream sequences described so far. The plasmid also has ...

journal_title：FEMS microbiology letters

authors： Sriprakash KS,Pearce BJ

更新日期：1990-09-15 00:00:00

abstract：:Clostridium difficile is the causative agent for pseudomembranous colitis in humans. Toxic strains of C. difficile produce two toxins, toxin A and toxin B. A reliable and definitive method of typing the toxic strains of C. difficile is needed since nosocomial cross infection is a primary concern in hospitals and other...

journal_title：FEMS microbiology letters

authors： McMillin DE,Muldrow LL

更新日期：1992-04-01 00:00:00

abstract：:Marine ammonia oxidising bacteria were enriched by incubation of sea water, amended with ammonium sulphate, and subsequent subculture in liquid inorganic medium. PCR primers were designed to be specific for rDNA sequences from ammonia oxidisers belonging to the beta-sub-group of the proteobacteria. These primers were ...

journal_title：FEMS microbiology letters

authors： McCaig AE,Embley TM,Prosser JI

更新日期：1994-07-15 00:00:00

abstract：:Rifampicin is a principal drug used to combat infections by mycobacteria and related organisms. Most strains of Mycobacterium are able to inactivate this antibiotic by ribosylation via an ADP-ribosylated intermediate. We found that this inactivation was inhibited by light at levels similar to those prevailing in labor...

journal_title：FEMS microbiology letters

authors： Dabbs ER,Quan S

更新日期：2000-01-01 00:00:00

abstract：:During the establishment of Escherichia coli O157:H7 infection, its capacity to adhere to host intestinal epithelial cells is the critical first step in pathogenesis. It also has the capability to form biofilms, and because both are surface activities, we sought to gain insight into a potential linkage between biofilm...

journal_title：FEMS microbiology letters

authors： Puttamreddy S,Minion FC

更新日期：2011-02-01 00:00:00

abstract：:Acetone-butanol-ethanol (ABE) fermentation is a metabolic process of clostridia that produces bio-based solvents including butanol. It is enabled by an underlying metabolic reaction network and modulated by cellular gene regulation and environmental cues. Mathematical modeling has served as a valuable strategy to faci...

journal_title：FEMS microbiology letters

authors： Liao C,Seo SO,Lu T

更新日期：2016-05-01 00:00:00

abstract：:Staphylococcus aureus produces a number of virulence factors. The major virulence factors exhibited by S aureus include various antigens, enzymes, cytotoxins and exotoxins (e.g. hemolysins, enterotoxins and toxic shock syndrome toxin). In this report, we show the influence of punicalagin on the secretion of exoprotein...

journal_title：FEMS microbiology letters

authors： Mun SH,Kong R,Seo YS,Zhou T,Kang OH,Shin DW,Kwon DY

更新日期：2016-11-01 00:00:00

abstract：:The ascomycete Sclerotinia sclerotiorum is a plant pathogen with a very broad host range. In order to identify and characterize genes involved in S. sclerotiorum infection of Brassica napus (canola), expressed sequence tags (ESTs) were examined from libraries prepared from three tissues: complex appressorium (infectio...

journal_title：FEMS microbiology letters

authors： Sexton AC,Cozijnsen AJ,Keniry A,Jewell E,Love CG,Batley J,Edwards D,Howlett BJ

更新日期：2006-05-01 00:00:00

abstract：:Trichothecenes are an important family of mycotoxins produced by several species of the genus Fusarium. These fungi cause serious disease on infected plants and postharvest storage of crops, and the toxins can cause health problems for humans and animals. Unfortunately, there are few methods for controlling mycotoxin ...

journal_title：FEMS microbiology letters

authors： Ismail Y,McCormick S,Hijri M

更新日期：2013-11-01 00:00:00