Identification of a glycolytic regulon in the archaea Pyrococcus and Thermococcus.


:The glycolytic pathway of the hyperthermophilic archaea that belong to the order Thermococcales (Pyrococcus, Thermococcus and Palaeococcus) differs significantly from the canonical Embden-Meyerhof pathway in bacteria and eukarya. This archaeal glycolysis variant consists of several novel enzymes, some of which catalyze unique conversions. Moreover, the enzymes appear not to be regulated allosterically, but rather at transcriptional level. To elucidate details of the gene expression control, the transcription initiation sites of the glycolytic genes in Pyrococcus furiosus have been mapped by primer extension analysis and the obtained promoter sequences have been compared with upstream regions of non-glycolytic genes. Apart from consensus sequences for the general transcription factors (TATA-box and BRE) this analysis revealed the presence of a potential transcription factor binding site (TATCAC-N(5)-GTGATA) in glycolytic and starch utilizing promoters of P. furiosus and several thermococcal species. The absence of this inverted repeat in Pyrococcus abyssi and Pyrococcus horikoshii probably reflects that their reduced catabolic capacity does not require this regulatory system. Moreover, this phyletic pattern revealed a TrmB-like regulator (PF0124 and TK1769) which may be involved in recognizing the repeat. This Thermococcales glycolytic regulon, with more than 20 genes, is the largest regulon that has yet been described for Archaea.


FEMS Microbiol Lett


van de Werken HJ,Verhees CH,Akerboom J,de Vos WM,van der Oost J




Has Abstract


2006-07-01 00:00:00














  • Cloning and characterization of the gene encoding penicillin-binding protein A of Streptomyces griseus.

    abstract::An internal segment of the penicillin-binding protein gene, pbpA, of Streptomyces griseus was amplified from genomic DNA using the polymerase chain reaction and used as a hybridization probe to isolate the complete gene from a cosmid library. pbpA encodes a 485 amino acid sequence that conserves three motifs of PBPs, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jiang H,Kendrick KE

    更新日期:2000-12-01 00:00:00

  • The neuA/flmD gene cluster of Helicobacter pylori is involved in flagellar biosynthesis and flagellin glycosylation.

    abstract::Helicobacter pylori possesses a gene (HP0326/JHP309) homologous to neuA of other bacteria, encoding a cytidyl monophosphate-N-acetylneuraminic acid synthetase-homologous enzyme in its N-terminal portion. We analysed the function of this gene, which is controlled by a flagellar class 2 sigma(54) promoter, in flagellar ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Josenhans C,Vossebein L,Friedrich S,Suerbaum S

    更新日期:2002-05-07 00:00:00

  • Quorum sensing and the anaerobic regulator (ANR) control polyhydroxyalkanoate (PHA) production in Pseudomonas chlororaphis PA23.

    abstract::Pseudomonas chlororaphis PA23 is a biocontrol agent that, in addition to producing antifungal compounds, synthesizes polyhydroxyalkanoate (PHA) polymers as a carbon and energy sink. Quorum sensing (QS) and the anaerobic regulator (ANR) are required for PA23-mediated fungal suppression; however, the role of these regul...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mohanan N,Gislason A,Sharma PK,Ghergab A,Plouffe J,Levin DB,de Kievit T

    更新日期:2019-09-01 00:00:00

  • Characterization of a novel vip3-type gene from Bacillus thuringiensis and evidence of its presence on a large plasmid.

    abstract::A novel vip3-type gene named vip3LB has been isolated from Bacillus thuringiensis strain BUPM95. The corresponding secreted vegetative insecticidal protein was active against the lepidopteran insect Ephestia kuehniella. The vip3LB gene was shown, for the first time, to be carried by the large plasmid containing the cr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mesrati LA,Tounsi S,Jaoua S

    更新日期:2005-03-15 00:00:00

  • A catabolic pathway for the degradation of chrysene by Pseudoxanthomonas sp. PNK-04.

    abstract::The chrysene-degrading bacterium Pseudoxanthomonas sp. PNK-04 was isolated from a coal sample. Three novel metabolites, hydroxyphenanthroic acid, 1-hydroxy-2-naphthoic acid and salicylic acid, were identified by TLC, HPLC and MS. Key enzyme activities, namely 1-hydroxy-2-naphthoate hydroxylase, 1,2-dihydroxynaphthalen...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Nayak AS,Sanjeev Kumar S,Santosh Kumar M,Anjaneya O,Karegoudar TB

    更新日期:2011-07-01 00:00:00

  • Determination of viridans streptococci surface lipoteichoic acid by enzyme linked immune sorbent assay.

    abstract::An enzyme linked immune sorbent assay (ELISA) was developed to measure bacterial surface lipoteichoic acid (LTA). Numerous strains of oral streptococci belonging to the 'viridans' group were examined on three separate occasions. The results show that, under these cultural conditions, oral streptococci do not normally ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hogg SD,Manning JE

    更新日期:1989-04-01 00:00:00

  • Surface Listeria monocytogenes carbohydrate-binding components revealed by agglutination with neoglycoproteins.

    abstract::Carbohydrate-binding components were shown to be present at the surface of Listeria monocytogenes by means of a panel of neoglycoproteins using direct agglutination. These lectin-like components bind on neoglycoproteins bearing D-glucosamine, L-fucosylamine, or para-amino-phenyl-alpha-D-mannopyrannoside residues. The ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cottin J,Loiseau O,Robert R,Mahaza C,Carbonnelle B,Senet JM

    更新日期:1990-03-15 00:00:00

  • The antibacterial effects of terpene alcohols on Staphylococcus aureus and their mode of action.

    abstract::The study was made of the antibacterial effects of three terpene alcohols on Staphylococcus aureus, focusing on the leakage of K+ ions and toxicity over time. The leakage of K+ ions was monitored continuously with a K+-electrode. Our results suggested that the terpene alcohols, namely, farnesol, nerolidol and plaunoto...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Inoue Y,Shiraishi A,Hada T,Hirose K,Hamashima H,Shimada J

    更新日期:2004-08-15 00:00:00

  • Variation and predicted structure of the flagellin gene in Actinoplanes species.

    abstract::Members of the genus Actinoplanes are considered to be representative of motile actinomycetes. To infer the flagellar diversity of Actinoplanes species, novel degenerate primers were designed for the flagellin (fliC) gene. The fliC gene of 21 Actinoplanes strains was successfully amplified and classified into two grou...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Yamamura H,Hanawa K,Kusunoki M,Nakamura K,Nakagawa Y,Ishida Y,Hamada M,Otoguro M,Tamura T,Fujita N,Hayakawa M

    更新日期:2011-11-01 00:00:00

  • The phosphotransferase system gene ptsI in the endophytic bacterium Bacillus cereus is required for biofilm formation, colonization, and biocontrol against wheat sharp eyespot.

    abstract::Natural resistance of wheat plants to wheat sharp eyespot is inadequate, and new strategies for controlling the disease are required. Biological control is an alternative and attractive way of reducing the use of chemicals in agriculture. In this study, we investigated the biocontrol properties of endophytic bacterium...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Xu YB,Chen M,Zhang Y,Wang M,Wang Y,Huang QB,Wang X,Wang G

    更新日期:2014-05-01 00:00:00

  • Response regulator, VemR, positively regulates the virulence and adaptation of Xanthomonas campestris pv. campestris.

    abstract::Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot disease in cruciferous plants. The synthesis of known virulence factors in this organism, such as extracellular enzymes and biofilm, is strictly regulated in response to environmental stimuli. Two-component signal transduction systems sense e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Tao J,He C

    更新日期:2010-03-01 00:00:00

  • Phylogenetic affinity of a Giardia lamblia cysteine desulfurase conforms to canonical pattern of mitochondrial ancestry.

    abstract::Among a few potential archezoan groups, only the Metamonada (diplomonads, retortamonads, and oxymonads) still retain the status of amitochondriate protists that diverged before the acquisition or retention of mitochondria. Indeed, finding that diplomonad genomes harbor a gene encoding a mitochondrial type chaperonin 6...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Emelyanov VV

    更新日期:2003-09-26 00:00:00

  • Structure of the Bordetella pertussis gene coding for the serotype 3 fimbrial subunit.

    abstract::Bordetella pertussis strains contain at least three distinct genes coding for fimbrial subunits, designated fim2, fim3, and fimX. The sequences of the fim2 and fimX genes have been published. Here we present the sequence of the fim3 gene. Proximal and distal to the fim3 gene, regions were observed that could function ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mooi FR,ter Avest A,van der Heide HG

    更新日期:1990-01-01 00:00:00

  • Isolation of transposon mutants from Azospirillum brasilense Yu62 and characterization of genes involved in indole-3-acetic acid biosynthesis.

    abstract::The molecular genetics of indole-3-acetic (IAA) synthesis and regulation in Azospirillum brasilense was investigated in this study. Tn5 mutagenesis was performed and five mutants with decreased IAA production were isolated. Five Tn5-inserted genes from these mutants were cloned and sequenced. Four genes were reported ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Xie B,Xu K,Zhao HX,Chen SF

    更新日期:2005-07-01 00:00:00

  • A role for carbon catabolite repression in the metabolism of phosphonoacetate by Agromyces fucosus Vs2.

    abstract::A strain of Agromyces fucosus, designated Vs2, metabolized a range of organophosphonate compounds as sole phosphorus sources for growth and metabolized phosphonoacetate as a sole carbon, energy and phosphorus source for growth. With phosphonoacetate as the sole phosphorus source and a pyruvate carbon source, transient...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: O'Loughlin SN,Graham RL,McMullan G,Ternan NG

    更新日期:2006-08-01 00:00:00

  • Cloning and expression of the D-ribulose-1,5-bisphosphate carboxylase/oxygenase form II gene from Thiobacillus intermedius in Escherichia coli.

    abstract::Both form I and II ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) genes were detected in Thiobacillus intermedius by heterologous hybridization using specific probes from Anacystis nidulans and Rhodobacter sphaeroides, respectively. However, only the previously reported form I enzyme could be demonstrated i...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Stoner MT,Shively JM

    更新日期:1993-03-01 00:00:00

  • Construction of a food-grade host/vector system for Lactococcus lactis based on the lactose operon.

    abstract::A plasmid-based food-grade vector system was developed for Lactococcus lactis by exploiting the genes for lactose metabolism. L. lactis MG5267 is a plasmid-free strain containing the entire lactose operon as a chromosomal insertion. The lacF gene was deleted from this strain by a double cross-over homologous recombina...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: MacCormick CA,Griffin HG,Gasson MJ

    更新日期:1995-03-15 00:00:00

  • Infection by the 'photosynthetic' phage S-PM2 induces increased synthesis of phycoerythrin in Synechococcus sp. WH7803.

    abstract::Phycoerythrin-containing Synechococcus strains are unicellular cyanobacteria that are of great ecological importance in the marine environment. These organisms are known to be susceptible to infection by cyanophages (viruses that infect cyanobacteria). The infection cycle takes several hours and during this time the c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shan J,Jia Y,Clokie MR,Mann NH

    更新日期:2008-06-01 00:00:00

  • Tetratricopeptide-like repeats in type-III-secretion chaperones and regulators.

    abstract::Efficient type-III secretion depends on cytosolic molecular chaperones, which bind specifically to the translocators and effectors. In the past there has been a tendency to shoe-horn all type-III-secretion chaperones into a single structural and functional class. However, we have shown that the LcrH/SycD-like chaperon...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Pallen MJ,Francis MS,Fütterer K

    更新日期:2003-06-06 00:00:00

  • Antifungal activity of three spermidine conjugates.

    abstract::Three tri-substituted spermidines, di-p-coumaroyl-caffeoylspermidine, tri-caffeoylspermidine and tri-p-coumaroylspermidine, isolated from pollen of Quercus alba, were examined for antifungal activity. Both di-p-coumaroyl-caffeoylspermidine and tri-p-coumaroylspermidine reduced mycelial growth of the oat leaf stripe pa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Walters D,Meurer-Grimes B,Rovira I

    更新日期:2001-07-24 00:00:00

  • A new root-nodulating symbiont of the tropical legume Sesbania, Rhizobium sp. SIN-1, is closely related to R. galegae, a species that nodulates temperate legumes.

    abstract::Rhizobium sp. SIN-1, isolated in India from root nodules on the tropical legume Sesbania aculeata, also induces nitrogen-fixing nodules on roots of S. macrocarpa, S. speciosa, S. procumbens, S. punicea, S. rostrata, and Vigna unguiculata. Unlike Azorhizobium caulinodans, SIN-1 does not induce stem nodules on S. rostra...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Rana D,Krishnan HB

    更新日期:1995-12-01 00:00:00

  • Helicobacter pylori strain ATCC700392 encodes a methyl-accepting chemotaxis receptor protein (MCP) for arginine and sodium bicarbonate.

    abstract::Helicobacter pylori ATCC43504 responds chemotactically to aspartic acid and serine, but not to arginine, nor to sodium bicarbonate. In contrast, H. pylori ATCC700392 (strain 26695) shows chemotaxis to all four attractants. Open reading frame HP0099 from H. pylori 26695 is predicted to encode one of three methyl-accept...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cerda O,Rivas A,Toledo H

    更新日期:2003-07-29 00:00:00

  • Regulation of the yeast vacuolar aminopeptidase Y gene (APY1) expression.

    abstract::The steady-state levels of the aminopeptidase Y (APY1) transcript and the level of assayable aminopeptidase Y activity were measured under a variety of nutritional conditions. Specific APY1 mRNA is less abundant in cells grown in minimal medium than in cells grown in rich medium, while active enzyme levels follow just...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Herrera-Camacho I,Suárez-Rendueles P

    更新日期:1996-06-01 00:00:00

  • Identification and characterization of a cross-reactive and a unique B-cell epitope on the hsp60 homologue from Neisseria gonorrhoeae.

    abstract::Two monoclonal antibodies (G6 and 7B), generated against a 63-kDa stress protein (GSP63) from Neisseria gonorrhoeae strain VP1, were used to investigate the antigenic heterogeneity of GSP63 among the Neisseriaceae and its antigenic relationship with the Hsp60 heat-shock protein family. Immunoblotting experiments demon...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Pannekoek Y,Dankert J,van Putten JP

    更新日期:1992-11-15 00:00:00

  • Ammonia-induced injury in pure cultures and natural populations of coliform bacteria.

    abstract::Ammonia-induced injury was investigated in pure cultures of Escherichia coli and Enterobacter aerogenes, and in natural coliform populations obtained from the oligotrophic Luxapallila and the eutrophic Sunflower Rivers in northern Mississippi. Pure cultures were affected by ammonia exposure as indicated by changes in ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Naundorf G,Aumen NG

    更新日期:1989-11-01 00:00:00

  • Bacterial differentiation, development, and disease: mechanisms for survival.

    abstract::Bacteria have the exquisite ability to maintain a precise diameter, cell length, and shape. The dimensions of bacteria size and shape are a classical metric in the distinction of bacterial species. Much of what we know about the particular morphology of any given species is the result of investigations of planktonic c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Justice SS,Harrison A,Becknell B,Mason KM

    更新日期:2014-11-01 00:00:00

  • Ku70 or Ku80 deficiencies in the fungus Botrytis cinerea facilitate targeting of genes that are hard to knock out in a wild-type context.

    abstract::The filamentous ascomycete Botrytis cinerea is one of the most studied models for understanding the necrotrophic behaviour of phytopathogenic fungi. The genomes of two strains of B. cinerea have been sequenced (B05.10 and T4), which may contribute to elucidating the virulence polymorphism in this fungus. In this study...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Choquer M,Robin G,Le Pêcheur P,Giraud C,Levis C,Viaud M

    更新日期:2008-12-01 00:00:00

  • Heat-inactivation of plasmid-encoded CI857 repressor induces gene expression from Ind- lambda prophage in recombinant Escherichia coli.

    abstract::We have observed significant cell lysis upon temperature up-shift of recombinant Escherichia coli cultures harboring CI857-repressed lambda-based expression vectors. This event, that becomes evident about 30-40 min after the heat shock, takes place when using the lambda promoter system in Ind- lysogenic strains, but n...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hoffmann F,Arís A,Carbonell X,Rohde M,Corchero JL,Rinas U,Villaverde A

    更新日期:1999-08-15 00:00:00

  • Nitrogen regulation in Corynebacterium glutamicum: isolation of genes involved and biochemical characterization of corresponding proteins.

    abstract::The regulation of nitrogen assimilation was investigated in the Gram-positive actinomycete Corynebacterium glutamicum. Biochemical studies and site-directed mutagenesis revealed that glutamine synthetase activity is regulated via adenylylation in this organism. The genes encoding the central signal transduction protei...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jakoby M,Krämer R,Burkovski A

    更新日期:1999-04-15 00:00:00

  • Molecular characterization of DNA encoding 23S rRNA and 16S-23S rRNA intergenic spacer regions of Aeromonas hydrophila.

    abstract::Amplification of the gene encoding 23S rRNA of Aeromonas hydrophila by polymerase chain reaction, with primers complementary to conserved regions of 16S and the 3'-end of 23S rRNA genes, resulted in a DNA fragment of approximately 3 kb. This fragment was cloned in Escherichia coli, and its nucleotide sequence determin...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: East AK,Collins MD

    更新日期:1993-01-15 00:00:00