Abstract:
:Mild alkaline solutions (beta-elimination), after removing the non-covalently bonded wall materials by hot SDS, released 13% and 26% of remaining wall proteins from mycelial and yeast cells of Candida albicans, respectively. When the beta-elimination was carried out after digestion of the walls with chitinase, four-fold more proteinaceous materials were released from mycelium and a similar amount in yeast walls. The solubilized materials were shown to be highly polydisperse, and endo-glycosidase H reduced their polydispersity and molecular masses, revealing different electrophoretic patterns in yeast and mycelial cell walls. The solubilized mycelial proteins carried N-glycosidic sugar chains and the epitopes recognized by two monoclonal antibodies were preserved, although showing a different behaviour in yeast walls. These results are consistent with the idea that significant amounts of intrinsic O-glycosylated mannoproteins are interconnected in the walls of C. albicans.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Mormeneo S,Marcilla A,Iranzo M,Sentandreu Rdoi
10.1111/j.1574-6968.1994.tb07212.xsubject
Has Abstractpub_date
1994-10-15 00:00:00pages
131-6issue
1-2eissn
0378-1097issn
1574-6968journal_volume
123pub_type
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