luxS in bacteria isolated from 25- to 40-million-year-old amber.

Abstract:

:Interspecies bacterial communication is mediated by autoinducer-2, whose synthesis depends on luxS. Due to the apparent universality of luxS (present in more than 40 bacterial species), it may have an ancient origin; however, no direct evidence is currently available. We amplified luxS in bacteria isolated from 25- to 40-million-year-old amber. The phylogenies and molecular clocks of luxS and the 16S rRNA gene from ancient and extant bacteria were determined as well. Luminescence assays using Vibrio harveyi BB170 aimed to determine the activity of luxS. While the phylogeny of luxS was very similar to that of extant Bacillus spp., amber isolates exhibited unique 16S rRNA gene phylogenies. This suggests that luxS may have been acquired by horizontal transfer millions of years ago. Molecular clocks of luxS suggest slow evolutionary rates, similar to those of the 16S rRNA gene and consistent with a conserved gene. Dendograms of the 16S rRNA gene and luxS show two separate clusters for the extant and ancient bacteria, confirming the uniqueness of the latter group.

journal_name

FEMS Microbiol Lett

authors

Santiago-Rodriguez TM,Patrício AR,Rivera JI,Coradin M,Gonzalez A,Tirado G,Cano RJ,Toranzos GA

doi

10.1111/1574-6968.12275

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

117-24

issue

1

eissn

0378-1097

issn

1574-6968

journal_volume

350

pub_type

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