Abstract:
:Interspecies bacterial communication is mediated by autoinducer-2, whose synthesis depends on luxS. Due to the apparent universality of luxS (present in more than 40 bacterial species), it may have an ancient origin; however, no direct evidence is currently available. We amplified luxS in bacteria isolated from 25- to 40-million-year-old amber. The phylogenies and molecular clocks of luxS and the 16S rRNA gene from ancient and extant bacteria were determined as well. Luminescence assays using Vibrio harveyi BB170 aimed to determine the activity of luxS. While the phylogeny of luxS was very similar to that of extant Bacillus spp., amber isolates exhibited unique 16S rRNA gene phylogenies. This suggests that luxS may have been acquired by horizontal transfer millions of years ago. Molecular clocks of luxS suggest slow evolutionary rates, similar to those of the 16S rRNA gene and consistent with a conserved gene. Dendograms of the 16S rRNA gene and luxS show two separate clusters for the extant and ancient bacteria, confirming the uniqueness of the latter group.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Santiago-Rodriguez TM,Patrício AR,Rivera JI,Coradin M,Gonzalez A,Tirado G,Cano RJ,Toranzos GAdoi
10.1111/1574-6968.12275subject
Has Abstractpub_date
2014-01-01 00:00:00pages
117-24issue
1eissn
0378-1097issn
1574-6968journal_volume
350pub_type
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