Carbon supply and 2-oxoglutarate effects on expression of nitrate reductase and nitrogen-regulated genes in Synechococcus sp. strain PCC 7942.

Abstract:

:Synthesis of nitrate reductase in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942 took place at a slow rate when the cells were incubated without a supply of inorganic carbon, but addition to these cells of CO(2)/bicarbonate or, in a Synechococcus strain transformed with a gene encoding a 2-oxoglutarate permease, 2-oxoglutarate stimulated expression of the enzyme. Induction by 2-oxoglutarate was also observed at the mRNA level for two nitrogen-regulated genes, nir and amt1, but not for the photosystem II D1 protein-encoding gene psbA1. Our results are consistent with a role of 2-oxoglutarate in nitrogen control in cyanobacteria.

journal_name

FEMS Microbiol Lett

authors

Vázquez-Bermúdez MF,Herrero A,Flores E

doi

10.1016/S0378-1097(03)00208-8

keywords:

subject

Has Abstract

pub_date

2003-04-25 00:00:00

pages

155-9

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378109703002088

journal_volume

221

pub_type

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