Identification of sequences important for recognition of vnf genes by the VnfA transcriptional activator in Azotobacter vinelandii.


:To analyze regulation of the vanadium-dependent nitrogenase of Azotobacter vinelandii, plasmids carrying vnfE-, vnfH-, or vnfD-lacZ fusions were transferred to Escherichia coli. These genes were expressed only if VnfA was present. Deletions of the vnfE upstream region were constructed and comparison of a region necessary for expression with sequences upstream of other vnf genes indicated a substantially conserved motif, GTAC-N6-GTAC, hypothesized to be the binding site for VnfA. This motif was duplicated with 17 or 18 bases lying between each in the vnfH and vnfD promoters. Deletion analysis of the vnfH promoter indicated that both motifs were necessary for full expression. In footprinting experiments, VnfA significantly protected from methylation the guanine residues within or immediately adjacent to the proposed VnfA recognition motifs. The active form of VnfA is probably interacting dimers, a tetramer, or a higher order oligomer since two regions of dyad symmetry are required for its interaction with the DNA.


FEMS Microbiol Lett


Woodley P,Buck M,Kennedy C




Has Abstract


1996-01-15 00:00:00












  • Horizontal transfer of nonconjugative plasmids in a colony biofilm of Escherichia coli.

    abstract::We tested the possibility of nonconjugative lateral DNA transfer in a colony biofilm of mixed Escherichia coli strains. By simply coculturing a plasmid-free F(-) strain and another F(-) strain harboring a nonconjugative plasmid in a colony biofilm on antibiotic-free agar media, transformed cells were produced within 2...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Maeda S,Ito M,Ando T,Ishimoto Y,Fujisawa Y,Takahashi H,Matsuda A,Sawamura A,Kato S

    更新日期:2006-02-01 00:00:00

  • Differences in effects on DNA gyrase activity between two glutamate racemases of Bacillus subtilis, the poly-gamma-glutamate synthesis-linking Glr enzyme and the YrpC (MurI) isozyme.

    abstract::Bacillus subtilis possesses two isogenes encoding glutamate racemases, the poly-gamma-glutamate synthesis-linking Glr enzyme and the YrpC isozyme, and produces abundant amounts of the Glr enzyme. The YrpC isozyme, but not the Glr enzyme, was found to influence the activity of DNA gyrase, as did the MurI-type glutamate...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ashiuchi M,Kuwana E,Komatsu K,Soda K,Misono H

    更新日期:2003-06-27 00:00:00

  • Genetic engineering of Streptococcus gordonii for the simultaneous display of two heterologous proteins at the bacterial surface.

    abstract::The Gram-positive bacterium Streptococcus gordonii has been genetically engineered to allow the simultaneous expression of two heterologous proteins at the cell surface. A family of recombinant streptococci displaying two different antigens was constructed. All the strains were genetically stable and expressed both pr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Maggi T,Spinosa M,Ricci S,Medaglini D,Pozzi G,Oggioni MR

    更新日期:2002-04-23 00:00:00

  • A role for carbon catabolite repression in the metabolism of phosphonoacetate by Agromyces fucosus Vs2.

    abstract::A strain of Agromyces fucosus, designated Vs2, metabolized a range of organophosphonate compounds as sole phosphorus sources for growth and metabolized phosphonoacetate as a sole carbon, energy and phosphorus source for growth. With phosphonoacetate as the sole phosphorus source and a pyruvate carbon source, transient...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: O'Loughlin SN,Graham RL,McMullan G,Ternan NG

    更新日期:2006-08-01 00:00:00

  • Physical and genetic map of Enterococcus faecium ATCC19434 and demonstration of intra- and interspecific genomic diversity in enterococci.

    abstract::A physical map of the Enterococcus faecium ATCC19434 chromosome was constructed by NotI, I-CeuI and Sse8387I. The chromosome was a circular DNA of 2600 kb in size, and contained six rRNA operons (rrn). The locations and orientations of the six rrn operons and 24 different determinants were mapped. Genomes of three add...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Oana K,Okimura Y,Kawakami Y,Hayashida N,Shimosaka M,Okazaki M,Hayashi T,Ohnishi M

    更新日期:2002-02-05 00:00:00

  • Characterization of monoclonal antibodies for rapid identification of Actinomyces naeslundii in clinical samples.

    abstract::The purpose of this study was to generate highly specific serological reagents for the quantitative identification of Actinomyces naeslundii in clinical samples, in particular dental plaque. Balb/c mice were immunized with pasteurized human A. naeslundii strains representing different genospecies and serotypes. Ten hy...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Thurnheer T,Guggenheim B,Gmür R

    更新日期:1997-05-15 00:00:00

  • Quantification of disease progression of several microbial pathogens on Arabidopsis thaliana using real-time fluorescence PCR.

    abstract::An accurate monitoring of disease progression is important to evaluate disease susceptibility phenotypes. Over the years, Arabidopsis thaliana has become the model species to serve as a host in plant-pathogen interactions. Despite the efforts to study genetic mechanisms of host defense, little efforts are made for a t...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Brouwer M,Lievens B,Van Hemelrijck W,Van den Ackerveken G,Cammue BP,Thomma BP

    更新日期:2003-11-21 00:00:00

  • Utilization of D-ribose through D-xylose transporter.

    abstract::An Escherichia coli mutant defective in high-affinity D-ribose transport is able to utilize the sugar as a sole carbon source, suggesting that other transport systems for D-ribose exist. In order to search for such transporters, transposon mutagenesis was carried out in an rbsB-negative strain containing ribokinase (r...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Song S,Park C

    更新日期:1998-06-15 00:00:00

  • Adherence of Candida albicans to components of the subendothelial extracellular matrix.

    abstract::Candida albicans yeasts adhered avidly to extracellular matrix (ECM) proteins, type IV collagen, laminin, and fibronectin immobilized on plastic. Type IV collagen showed an increase of adherence of 400% above control values; laminin, 300%; and fibronectin, 150%. In addition, all three (in quantities of 0.02-200 microg...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Klotz SA

    更新日期:1990-03-15 00:00:00

  • Rapid purification of the peptide toxins microcystin-LR and nodularin.

    abstract::An isolation procedure was developed to provide within one day microcystin-LR, a cyclic heptapeptide toxin from Microcystis aeruginosa PCC 7806. After ODS (octadecylsilyl) solid phase extraction, the crude toxin fraction was chromatographed using a strong anion exchange column. The toxin was eluted with 0.02 M ammoniu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Martin C,Sivonen K,Matern U,Dierstein R,Weckesser J

    更新日期:1990-03-01 00:00:00

  • Bacillus and Streptomyces were selected as broad-spectrum antagonists against soilborne pathogens from arid areas in Egypt.

    abstract::Plant protection via disease-suppressive bacteria in desert farming requires specific biological control agents (BCAs) adapted to the unique arid conditions. We performed an ecological study of below-ground communities in desert farm soil and untreated desert soil, and based on these findings, selected antagonists wer...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Köberl M,Ramadan EM,Adam M,Cardinale M,Hallmann J,Heuer H,Smalla K,Berg G

    更新日期:2013-05-01 00:00:00

  • Characterization of tccP2 carried by atypical enteropathogenic Escherichia coli.

    abstract::Atypical enteropathogenic Escherichia coli (EPEC) comprise an important group of paediatric pathogens. Atypical EPEC have reservoirs in farm and domestic animals where they can be either commensal or pathogenic; serogroup O26 is dominant in humans and animals. Central to intestinal colonization by EPEC is the transloc...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ooka T,Vieira MA,Ogura Y,Beutin L,La Ragione R,van Diemen PM,Stevens MP,Aktan I,Cawthraw S,Best A,Hernandes RT,Krause G,Gomes TA,Hayashi T,Frankel G

    更新日期:2007-06-01 00:00:00

  • A surface polysaccharide forms when gonococci are converted to serum resistance by cytidine 5'-monophospho-N-acetyl neuraminic acid.

    abstract::A serum-susceptible, guinea-pig chamber-passaged, laboratory strain (BS4 (agar)) of Neisseria gonorrhoeae was converted to serum resistance by incubation with cytidine 5-monophospho-N-acetyl neuraminic acid (CMP-NANA) and examined by electron microscopy after staining with ruthenium-red. In contrast to serum susceptib...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fox AJ,Curry A,Rowland PL,Lancaster S,Jones DM,Parsons NJ,Cole JA,Smith H

    更新日期:1990-01-01 00:00:00

  • Accumulation of an artificial cell wall-binding lipase by Bacillus subtilis wprA and/or sigD mutants.

    abstract::A recombinant lipase, CWB-LipB, localized on the Bacillus subtilis cell surface and retaining lipase activity was unstable and not accumulated in a high yield. To improve the accumulation, we examined cell wall binding protease (wprA)- and/or sigma D (sigD)-deficient mutants, and also a NprE and AprA protease-deficien...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kobayashi G,Toida J,Akamatsu T,Yamamoto H,Shida T,Sekiguchi J

    更新日期:2000-07-15 00:00:00

  • Inhibition of adhesion-promoting activity of a human salivary protein which promotes adhesion of Streptococcus mutans JBP to hydroxyapatite.

    abstract::The adhesion-promoting proteins (APP) (molecular mass approx. 300 kDa), which promote adhesion of Streptococcus mutans JBP (serotype c) to hydroxyapatite, were isolated from human submandibular-sublingual (SMSL) saliva by gel filtration on a Trisacryl GP2000 M column. The effects of hexoses, pentoses, methyl-pentoses,...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kishimoto E,Hay DI,Gibbons RJ

    更新日期:1991-12-15 00:00:00

  • A calcium and calmodulin-dependent protein kinase present in differentiating Dictyostelium discoideum.

    abstract::A protein kinase from Dictyostelium discoideum which phosphorylates the synthetic peptide, calmodulin-dependent protein kinase substrate (CDPKS, amino acid sequence: PLRRTLSVAA) and is stimulated by Ca2+/calmodulin is described. This is the first report of a protein kinase with these characteristics in D. discoideum. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dunbar AJ,Wheldrake JF

    更新日期:1994-01-01 00:00:00

  • Genetic evidence for a novel thymidylate synthase in the halophilic archaeon Halobacterium salinarum and in Campylobacter jejuni.

    abstract::A search of the complete genome sequence of the halophilic archaeon Halobacterium salinarum failed to identify a gene homologous to the thymidylate synthase (thyA) gene present in the closely related Haloferax volcanii. To understand the source of thymidine synthesis in Hbt. salinarum, a genomic library of Hbt. salina...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Giladi M,Bitan-Banin G,Mevarech M,Ortenberg R

    更新日期:2002-10-29 00:00:00

  • Magnaporthe oryzae endopolygalacturonase homolog correlates with density-dependent conidial germination.

    abstract::Endopolygalacturonases (endoPGs) of some phytopathogens are virulent factors for dicots. To investigate the function of the endoPG of Magnaporthe oryzae, a disruption mutant of MGG_08938, the homolog of endoPG found in the genome database of this fungus, was generated. The pathogenicity, mycelial growth, and appressor...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mori T,Jung HY,Maejima K,Hirata H,Himeno M,Hamamoto H,Namba S

    更新日期:2008-03-01 00:00:00

  • Incorporation of choline into Streptococcus pneumoniae cell wall antigens: evidence for choline kinase activity.

    abstract::The choline-containing teichoic and lipoteichoic acids play an important part in cell wall metabolism of Streptococcus pneumoniae. We propose that a choline kinase enzyme has a role in the synthesis of these antigens. The presence of this enzyme was demonstrated in cell free extracts of S. pneumoniae by measuring the ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Whiting GC,Gillespie SH

    更新日期:1996-05-01 00:00:00

  • A combined stress response analysis of Spirulina platensis in terms of global differentially expressed proteins, and mRNA levels and stability of fatty acid biosynthesis genes.

    abstract::Changes in gene expression play a critical role in enhancing the ability of cyanobacteria to survive under cold conditions. In the present study, Spirulina platensis cultures were grown at the optimal growth temperature, in the light, before being transferred to dark conditions at 22 degrees C. Two dimensional-differe...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jeamton W,Mungpakdee S,Sirijuntarut M,Prommeenate P,Cheevadhanarak S,Tanticharoen M,Hongsthong A

    更新日期:2008-04-01 00:00:00

  • The subcellular localization of a C-terminal processing protease in Pseudomonas aeruginosa.

    abstract::Carboxy (C)-terminal processing proteases (CTP) are a relatively new group of serine proteases. Found in a broad range of organisms - bacteria, archaea, algae, plants and animals - these proteases are involved in the C-terminal processing of proteins. In comparison with amino-terminal processing of bacterial proteins,...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hoge R,Laschinski M,Jaeger KE,Wilhelm S,Rosenau F

    更新日期:2011-03-01 00:00:00

  • Expression of the phytochrome operon in Pseudomonas aeruginosa is dependent on the alternative sigma factor RpoS.

    abstract::Phytochromes are red/far-red light photoreceptors found in plants, cyanobacteria and heterotrophic bacteria. Biochemical analyses have established that the genes bphO and bphP (PA4116 and PA4117) of Pseudomonas aeruginosa encode both phytochrome components: BphO, a heme oxygenase that produces the linear tetrapyrrole ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Barkovits K,Harms A,Benkartek C,Smart JL,Frankenberg-Dinkel N

    更新日期:2008-03-01 00:00:00

  • Purification and characterisation of a quorum quenching AHL-lactonase from the endophytic bacterium Enterobacter sp. CS66.

    abstract::The quorum quenching (QQ) activity of endophytic bacteria associated with medicinal plants was explored. Extracts of the Gram-negative Enterobacter sp. CS66 possessed potent N-acylhomoserine lactone (AHL) hydrolytic activity in vitro. Using degenerate primers, we PCR-amplified an open reading frame (denoted aiiE) from...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shastry RP,Dolan SK,Abdelhamid Y,Vittal RR,Welch M

    更新日期:2018-05-01 00:00:00

  • Internal size variations in Tn1546-like elements due to the presence of IS1216V.

    abstract::In this study, internal size variations in the VanA gene cluster Tn1546, encoding resistance to glycopeptides, is described. Studies of previously uncharacterized size variations of an internal region, encoding the vanX and vanY genes of Tn1546, revealed that these variations were due to the presence of the IS sequenc...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jensen LB

    更新日期:1998-12-15 00:00:00

  • The pel1 mutant of Saccharomyces cerevisiae is deficient in cardiolipin and does not survive the disruption of the CHO1 gene encoding phosphatidylserine synthase.

    abstract::Cells of the pel1 mutant of Saccharomyces cerevisiae were found to contain an extremely low content of cardiolipin, a decreased level of phosphatidylcholine and an increased level of phosphatidylinositol. Disruption of the PEL1 gene in cells containing a null mutation in the CHO1 gene was lethal. Despite its putative ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Janitor M,Obernauerová M,Kohlwein SD,Subík J

    更新日期:1996-06-15 00:00:00

  • Identification of Mga1, a G-protein alpha-subunit gene involved in regulating citrinin and pigment production in Monascus ruber M7.

    abstract::The filamentous fungi Monascus spp., which have been used in traditional fermented food in Asia for centuries, are well-known producers of a group of bioactive metabolites that are widely used as food additives and nutraceutical supplements worldwide. However, its potential to produce the mycotoxin citrinin poses a th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Li L,Shao Y,Li Q,Yang S,Chen F

    更新日期:2010-07-01 00:00:00

  • Infection by the 'photosynthetic' phage S-PM2 induces increased synthesis of phycoerythrin in Synechococcus sp. WH7803.

    abstract::Phycoerythrin-containing Synechococcus strains are unicellular cyanobacteria that are of great ecological importance in the marine environment. These organisms are known to be susceptible to infection by cyanophages (viruses that infect cyanobacteria). The infection cycle takes several hours and during this time the c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shan J,Jia Y,Clokie MR,Mann NH

    更新日期:2008-06-01 00:00:00

  • Characterisation of the bovine enteric calici-like virus, Newbury agent 1.

    abstract::The bovine enteric calici-like virus, Newbury agent 1 (NA1) was characterised to determine if it is a member of the Caliciviridae and to establish its antigenic relationship to the established bovine enteric calicivirus Newbury agent 2 (NA2). Solid phase immune electron microscopy (SPIEM) allowed quantification of NA1...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dastjerdi AM,Snodgrass DR,Bridger JC

    更新日期:2000-11-01 00:00:00

  • The Serratia-type hemolysin of Chromobacterium violaceum.

    abstract::Chromobacterium violaceum is a Gram-negative opportunistic human pathogen and an inhabitant of tropical soils and waterways. Although known primarily for the synthesis of the pigment violacein, and more recently as a reporter strain for quorum sensing, clinical reports of chromobacteriosis comprise the largest block o...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Brumbach KC,Eason BD,Anderson LK

    更新日期:2007-02-01 00:00:00

  • Nuclear behavior in fungal hyphae.

    abstract::A characteristic feature of fungal hypha is the presence of large number of nuclei in a common cytoplasmic environment. Where it has been examined, the coenocytic mycelium is commonly heterokaryotic. The nuclei cooperate, compete or combat. It is proposed that in addition to their classical role in heredity, supernume...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Maheshwari R

    更新日期:2005-08-01 00:00:00