Identification of sequences important for recognition of vnf genes by the VnfA transcriptional activator in Azotobacter vinelandii.


:To analyze regulation of the vanadium-dependent nitrogenase of Azotobacter vinelandii, plasmids carrying vnfE-, vnfH-, or vnfD-lacZ fusions were transferred to Escherichia coli. These genes were expressed only if VnfA was present. Deletions of the vnfE upstream region were constructed and comparison of a region necessary for expression with sequences upstream of other vnf genes indicated a substantially conserved motif, GTAC-N6-GTAC, hypothesized to be the binding site for VnfA. This motif was duplicated with 17 or 18 bases lying between each in the vnfH and vnfD promoters. Deletion analysis of the vnfH promoter indicated that both motifs were necessary for full expression. In footprinting experiments, VnfA significantly protected from methylation the guanine residues within or immediately adjacent to the proposed VnfA recognition motifs. The active form of VnfA is probably interacting dimers, a tetramer, or a higher order oligomer since two regions of dyad symmetry are required for its interaction with the DNA.


FEMS Microbiol Lett


Woodley P,Buck M,Kennedy C




Has Abstract


1996-01-15 00:00:00












  • Analysis of the molecular mass heterogeneity of the transferrin receptor in Neisseria meningitidis and commensal Neisseria.

    abstract::Peroxidase-conjugated transferrin was used to detect transferrin receptors both in intact outer membrane vesicles (OMVs) from Neisseria species in a dot blot assay, and in SDS-PAGE-separated OMV proteins after transferring to nitrocellulose membranes. All N. meningitidis strains produced transferrin receptors after cu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ferreirós CM,Criado MT,Pintor M,Ferrón L

    更新日期:1991-10-15 00:00:00

  • Identification and characterization of a cross-reactive and a unique B-cell epitope on the hsp60 homologue from Neisseria gonorrhoeae.

    abstract::Two monoclonal antibodies (G6 and 7B), generated against a 63-kDa stress protein (GSP63) from Neisseria gonorrhoeae strain VP1, were used to investigate the antigenic heterogeneity of GSP63 among the Neisseriaceae and its antigenic relationship with the Hsp60 heat-shock protein family. Immunoblotting experiments demon...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Pannekoek Y,Dankert J,van Putten JP

    更新日期:1992-11-15 00:00:00

  • Properties of the periplasmic nitrate reductases from Paracoccus pantotrophus and Escherichia coli after growth in tungsten-supplemented media.

    abstract::Paracoccus pantotrophus grown anaerobically under denitrifying conditions expressed similar levels of the periplasmic nitrate reductase (NAP) when cultured in molybdate- or tungstate-containing media. A native PAGE gel stained for nitrate reductase activity revealed that only NapA from molybdate-grown cells displayed ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gates AJ,Hughes RO,Sharp SR,Millington PD,Nilavongse A,Cole JA,Leach ER,Jepson B,Richardson DJ,Butler CS

    更新日期:2003-03-28 00:00:00

  • Efficient extracellular production of hybrid E. coli heat-labile enterotoxin B subunits in a marine Vibrio.

    abstract::Escherichia coli heat-labile enterotoxin B subunit (EtxB) has been proposed as a potential protein carrier for the delivery of heterologous peptides to target cells, particularly for the oral delivery of epitopes to the mucosal immune system. In this study, two extensions to the C-terminus of EtxB were genetically eng...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Marcello A,Loregian A,Palù G,Hirst TR

    更新日期:1994-03-15 00:00:00

  • Different in vivo localization of the Escherichia coli proteins CspD and CspA.

    abstract::Two Csp proteins (CspA and CspD) were fused to the green fluorescent protein GFP and expressed from their natural promoters or from an inducible promoter. Fluorescence microscopy and computerized image analysis indicate that in Escherichia coli growing at 37 degrees C CspD localizes in the nucleoid like the control H-...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Giangrossi M,Exley RM,Le Hegarat F,Pon CL

    更新日期:2001-08-21 00:00:00

  • Presence of non-suppressive, M2-related dsRNAs molecules in Saccharomyces cerevisiae strains isolated from spontaneous fermentations.

    abstract::Total dsRNA extractions in five killer K2 strains of Saccharomyces cerevisiae isolated from spontaneous fermentations revealed the presence of a novel dsRNA fragment (which we named NS dsRNA) of approximately 1.30 kb, together with L and M2 dsRNAs. NS dsRNA appeared to be encapsidated in the same kind of viral particl...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cansado J,Barros Velázquez J,Sieiro C,Gacto M,Villa TG

    更新日期:1999-12-15 00:00:00

  • Survival of nontypeable Haemophilus influenzae in macrophages.

    abstract::In this study we have investigated the ability of nonencapsulated, nontypeable Haemophilus influenzae, NT477 to survive in the J774 mouse macrophage-like cell line. Viable, intracellular nontypeable H. influenzae could still be recovered from macrophages 72 h after phagocytosis. In contrast, H. influenzae strain Rd, a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Craig JE,Cliffe A,Garnett K,High NJ

    更新日期:2001-09-11 00:00:00

  • A multiplex-PCR for the differentiation of Mycobacterium bovis and Mycobacterium tuberculosis.

    abstract::A multiplex-polymerase chain reaction (PCR) assay based on one-step amplification and detection of two different mycobacterial genomic fragments was designed for differentiation of Mycobacterium bovis and Mycobacterium tuberculosis. The oligonucleotide primers were chosen from a 500-bp genomic fragment which is well c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shah DH,Verma R,Bakshi CS,Singh RK

    更新日期:2002-08-27 00:00:00

  • Development and evaluation of a rapid, simple, sensitive, monoclonal antibody-based co-agglutination test for direct detection of Vibrio cholerae 01.

    abstract::Cholera epidemics caused by Vibrio cholerae 01 continue to represent a major public health concern in many developing countries. A rapid and simple test kit for the detection of V. cholerae 01 has been developed. The kit, CholeraScreen is a monoclonal antibody-based, co-agglutination test and is used directly with sto...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Colwell RR,Hasan JA,Huq A,Loomis L,Siebeling RJ,Torres M,Galvez S,Islam S,Tamplin MT,Bernstein D

    更新日期:1992-10-15 00:00:00

  • Chromosomal analysis of group B streptococcal clinical strains; bac gene-positive strains are genetically homogenous.

    abstract::A collection of 45 epidemiologically unrelated Streptococcus agalactiae strains (group B Streptococcus, GBS), belonging to different serotypes, isolated from pregnant women in China and Russia was studied. Strains were characterized by pulsed-field gel electrophoresis (PFGE) employing hybridization with nine genes pot...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dmitriev A,Hu YY,Shen AD,Suvorov A,Yang YH

    更新日期:2002-02-19 00:00:00

  • Molecular characterization of DNA encoding 23S rRNA and 16S-23S rRNA intergenic spacer regions of Aeromonas hydrophila.

    abstract::Amplification of the gene encoding 23S rRNA of Aeromonas hydrophila by polymerase chain reaction, with primers complementary to conserved regions of 16S and the 3'-end of 23S rRNA genes, resulted in a DNA fragment of approximately 3 kb. This fragment was cloned in Escherichia coli, and its nucleotide sequence determin...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: East AK,Collins MD

    更新日期:1993-01-15 00:00:00

  • Secretory production of Arthrobacter levan fructotransferase from recombinant Escherichia coli.

    abstract::Levan fructotransferase (LFTase) from Arthrobacter ureafaciens K2032 was expressed with N-terminal fusion of a LacZ-derived secretion motif (TMITNSSSVP) using the lac promoter system in recombinant Escherichia coli JM109 [pUDF-A81]. In flask cultures, recombinant enzyme activity was detected in culture media, and sequ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lee J,Saraswat V,Koh I,Song KB,Park YH,Rhee SK

    更新日期:2001-02-20 00:00:00

  • Effect of pheromone induction on transfer of the Enterococcus faecalis plasmid pCF10 in intestinal mucus ex vivo.

    abstract::The effect of synthetic sex pheromone on pheromone-inducible conjugation between the isogenic Enterococcus faecalis strains OG1RF and OG1SS was investigated in (i) Todd-Hewitt broth medium and (ii) intestinal mucus isolated from germ-free rats. In broth, the presence of synthetic pheromone cCF10 had no detectable effe...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Licht TR,Hammerum AM,Jensen LB,Jacobsen BL

    更新日期:2001-11-13 00:00:00

  • Identification of a new promoter for the response regulator rcsB expression in Salmonella enterica serovar Typhimurium.

    abstract::The RcsCDB (Rcs) phosphorelay system regulates capsule synthesis, flagella production and other cellular activities in several enteric bacteria. This system consists of three proteins: the sensor RcsC, the cognate response regulator RcsB and the histidine-containing phosphotransfer protein RcsD (YojN), which is hypoth...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Pescaretti Mde L,Morero R,Delgado MA

    更新日期:2009-11-01 00:00:00

  • An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda Red.

    abstract::We have established an improved large deletion method in Escherichia coli genome using a combination of two different recombination systems, lambda Red and Cre/loxP. The loxP site could be rapidly and efficiently integrated in the genome by lambda Red and large deletions of both 117- and 165-kbp regions could be gener...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fukiya S,Mizoguchi H,Mori H

    更新日期:2004-05-15 00:00:00

  • Multicopy suppression of a gacA mutation by the infC operon in Pseudomonas fluorescens CHA0: competition with the global translational regulator RsmA.

    abstract::The gacA gene of the biocontrol strain Pseudomonas fluorescens CHA0 codes for a response regulator which, together with the sensor kinase GacS (=LemA), is required for the production of exoenzymes and secondary metabolites involved in biocontrol, including hydrogen cyanide (HCN). A gacA multicopy suppressor was isolat...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Blumer C,Haas D

    更新日期:2000-06-01 00:00:00

  • The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling.

    abstract::Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) represents a simple reliable approach for rapid bacterial identification based on specific peptide/protein fingerprints. However, cell-wall characteristics of mycobacterial species, and their well known stability, complicate MA...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Balážová T,Makovcová J,Šedo O,Slaný M,Faldyna M,Zdráhal Z

    更新日期:2014-04-01 00:00:00

  • Distribution of the antiseptic-resistance genes qacE and qacE delta 1 in gram-negative bacteria.

    abstract::The distribution of the antiseptic-resistance genes qacE and qacE delta 1 was studied in a large number of Gram-negative bacteria by a method that included the polymerase chain reaction (PCR). A total of 117 strains of Gram-negative bacteria, isolated from clinical or environmental sources, was used in this analysis. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kazama H,Hamashima H,Sasatsu M,Arai T

    更新日期:1998-02-15 00:00:00

  • Molecular characterization of benzimidazole-resistant isolates of Cladosporium fulvum.

    abstract::The benzimidazole fungicide thiophanate-methyl is commonly applied to control leaf mould of tomato caused by Cladosporium fulvum in China. In this study, 32 isolates of C. fulvum were examined for their sensitivities to thiophanate-methyl, and two benzimidazole-resistant (BenR) phenotypes BenR1 and BenR2 were identifi...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Yan L,Chen J,Zhang C,Ma Z

    更新日期:2008-01-01 00:00:00

  • GalK limits type I-F CRISPR-Cas expression in a CRP-dependent manner.

    abstract::CRISPR-Cas adaptive immune systems protect bacteria from phage predation, and other foreign genetic elements such as plasmids. Significant advances have been made regarding how CRISPR-Cas systems elicit immunity; however, comparatively little is known about their regulation. To study CRISPR-Cas regulation, we describe...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hampton HG,Patterson AG,Chang JT,Taylor C,Fineran PC

    更新日期:2019-06-01 00:00:00

  • Bacillus cereus and its food poisoning toxins.

    abstract::Bacillus cereus is becoming one of the more important causes of food poisoning in the industrialised world. It produces one emetic toxin and three different enterotoxins. The emetic toxin is a ring-shaped structure of three repeats of four amino and/or oxy acids: [D-O-Leu-D-Ala-L-O-Val-L-Val]3. This ring structure has...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Granum PE,Lund T

    更新日期:1997-12-15 00:00:00

  • A chemiluminescence immunoassay for evaluation of Cryptosporidium parvum growth in vitro.

    abstract::A chemiluminescence immunoassay (CLIA) was developed to detect Cryptosporidium parvum growth in Madin-Darby canine kidney (MDCK) cell cultures. Optimal results were obtained when MDCK cells were plated at a density of 1 x 10(4) cells/well (96-well plate) and maintained as a monolayer for 4 days prior to infection with...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: You X,Arrowood MJ,Lejkowski M,Xie L,Schinazi RF,Mead JR

    更新日期:1996-03-01 00:00:00

  • Morphological and intracellular alterations induced by cytotoxin VT2y produced by Escherichia coli isolated from chickens with swollen head syndrome.

    abstract::Recently, a novel verocytotoxin named VT2y was described which belongs to the STx family and is produced by Escherichia coli isolated from domestic poultry with swollen head syndrome (SHS). The VT2y toxin induced apoptosis in Vero, HeLa, CHO, CEF (primary chicken embryo fibroblast) and PCK (primary chicken kidney) cel...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Salvadori MR,Yamada AT,Yano T

    更新日期:2001-04-01 00:00:00

  • Replicating function of the RS1 element associated with Vibrio cholerae CTX phi prophage.

    abstract::The RS1 element associated with Vibrio cholerae CTX phi prophage was cloned from an E1 Tor biotype Vibrio cholerae strain. We used the recA- vaccine strain Peru-15, that lacks the target for RS-mediated site-specific integration, to show that RS1 promotes autonomous replication of a suicide vector. A linker insertion ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Campos J,Fando R,Silva A,Rodriguez BL,Benitez JA

    更新日期:1998-07-01 00:00:00

  • Immunogenicity of IMS 1113 plus soluble subunit and chimeric proteins containing Mycoplasma hyopneumoniae P97 C-terminal repeat regions.

    abstract::The surface adhesin P97 mediates the adherence of Mycoplasma hyopneumoniae to swine cilia. Two reiterated repeats R1 and R2 are located at the C-terminus of P97. The purpose of this study was to evaluate the immunogenicity of Montanide adjuvant IMS 1113 plus soluble subunit proteins rR1, rR1R2 and their chimeric forms...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Barate AK,Cho Y,Truong QL,Hahn TW

    更新日期:2014-03-01 00:00:00

  • Production of minicellulosomes from Clostridium cellulovorans for the fermentation of cellulosic ethanol using engineered recombinant Saccharomyces cerevisiae.

    abstract::Saccharomyces cerevisiae was engineered for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum. The chimeric endoglucanase E fused with the dockerin domain of endoglucanase B from C. ce...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hyeon JE,Yu KO,Suh DJ,Suh YW,Lee SE,Lee J,Han SO

    更新日期:2010-09-01 00:00:00

  • Arginine inhibits Na-driven flagellar motors of alkaliphilic Bacillus.

    abstract::L-arginine has attracted a great deal of attention as an agent for refolding denatured proteins, and the mildness of its effects offer hope for a wide range of potential applications for this substance, including medicines with few side effects. We report that both L- and D-arginine inhibits Na+-driven flagellar motor...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Atsumi T,Yoshimura F,Sugiyama S

    更新日期:2006-03-01 00:00:00

  • Corynebacterium diphtheriae surface proteins as adhesins to human erythrocytes.

    abstract::Corynebacterium diphtheriae strains express non-fimbrial surface proteins able to recognize and bind to specific host cells receptors. Protein extracts were obtained from bacterial cells by mechanical process and ammonium sulfate precipitation at 25 and 45% (w/v) saturation. SDS-PAGE analysis of the extracts detected ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Colombo AV,Hirata R Jr,de Souza CM,Monteiro-Leal LH,Previato JO,Formiga LC,Andrade AF,Mattos-Guaraldi AL

    更新日期:2001-04-13 00:00:00

  • Solubilization of zinc salts by a bacterium isolated from the air environment of a tannery.

    abstract::Airborne bacteria isolated from a tannery air environment were screened for the property of solubilization of insoluble zinc oxide and zinc phosphate. Out of 10 strains tested, a strain of Pseudomonas aeruginosa (CMG 823) showed the best solubilization and solubilized both zinc oxide and zinc phosphate. Colonies of th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fasim F,Ahmed N,Parsons R,Gadd GM

    更新日期:2002-07-16 00:00:00

  • Proteomic analysis of the insoluble subproteome of Clostridium difficile strain 630.

    abstract::Clostridium difficile, a Gram-positive spore-forming anaerobe, causes infections in humans ranging from mild diarrhoeal to potentially life-threatening pseudomembranous colitis. The availability of genomic information for a range of C. difficile strains affords researchers the opportunity to better understand not only...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jain S,Graham RL,McMullan G,Ternan NG

    更新日期:2010-11-01 00:00:00