Identification of sequences important for recognition of vnf genes by the VnfA transcriptional activator in Azotobacter vinelandii.

abstract：:The genus Burkholderia comprises over 28 species and species-specific, recA-based polymerase chain reaction (PCR) tests are available for several species, but not for some soil-inhabiting species including B. fungorum. Previous analysis of several novel rhizospheric, environmental isolates belonging to the B. cepacia ...

journal_title：FEMS microbiology letters

authors： Chan CH,Stead DE,Coutts RH

更新日期：2003-07-15 00:00:00

abstract：:The extensively discussed idea of oxidative stress development under antibiotic treatment was confirmed using an antioxidant gene expression (soxRS-, oxyR-regulon) approach, including microaerobic cultivation conditions. The killing action of antibiotics and their ability to cause peroxide oxidative stress in Escheric...

journal_title：FEMS microbiology letters

authors： Akhova AV,Tkachenko AG

更新日期：2014-04-01 00:00:00

abstract：:The study was made of the antibacterial effects of three terpene alcohols on Staphylococcus aureus, focusing on the leakage of K+ ions and toxicity over time. The leakage of K+ ions was monitored continuously with a K+-electrode. Our results suggested that the terpene alcohols, namely, farnesol, nerolidol and plaunoto...

journal_title：FEMS microbiology letters

authors： Inoue Y,Shiraishi A,Hada T,Hirose K,Hamashima H,Shimada J

更新日期：2004-08-15 00:00:00

abstract：:Based on amino acid sequence similarities between the methylated elongation factor EF-Tu from Escherichia coli and the EF-Tu from Euglena gracilis chloroplast, we predicted that the latter could also be methylated in the presence of an appropriate methyltransferase. We found that, as reported for the eubacterial homol...

journal_title：FEMS microbiology letters

authors： Toledo H,Jerez CA

更新日期：1990-09-15 00:00:00

abstract：:We identified the minimal locus of 163-kb plasmid pSV1 of Streptomyces violaceoruber for the replication in S. lividans. This locus comprised a repA gene and an upstream 407-bp sequence containing two inverted repeats (IR-III and IR-IV) within an iteron, an AT-rich region and a 300-bp noncoding sequence (NCS). RepA pr...

journal_title：FEMS microbiology letters

authors： Chen Y,Tan H,Qin Z

更新日期：2013-12-01 00:00:00

abstract：:Occurrence of tetracycline resistance genes encoding ribosomal protection proteins was examined in 151 tetracycline-resistant bacterial isolates from fish and seawater at coastal aquaculture sites in Japan and Korea. The tet(M) gene was detected in 34 Japanese and Korean isolates, which included Vibrio sp., Lactococcu...

journal_title：FEMS microbiology letters

authors： Kim SR,Nonaka L,Suzuki S

更新日期：2004-08-01 00:00:00

abstract：:The molecular mechanisms controlling expression of the long polar fimbriae 2 (Lpf2) of enterohemorrhagic Escherichia coli (EHEC) O157:H7 were evaluated. Primer extension was used to locate the lpfA2 transcriptional start site in EHEC strain EDL933 at 171 bp upstream of the lpfA2 start codon. Semi-quantitative RT-PCR d...

journal_title：FEMS microbiology letters

authors： Arenas-Hernández MM,Rojas-López M,Medrano-López A,Nuñez-Reza KJ,Puente JL,Martínez-Laguna Y,Torres AG

更新日期：2014-08-01 00:00:00

abstract：:The flaA gene, which codes for the Spirochaeta aurantia flagellar filament outer layer polypeptide, FlaA, was cloned, sequenced and analysed. The gene appears to be transcribed into a monocistronic mRNA from a sigma 70-like promoter. The translational start is 31 base pairs after the start of the transcript, the open ...

journal_title：FEMS microbiology letters

authors： Parales J Jr,Greenberg EP

更新日期：1993-02-01 00:00:00

abstract：:In Corynebacterium glutamicum the promoter of the araBAD Escherichia coli gene is positively regulated by both arabinose and the araC gene product, as it is the case in the natural host. If the L-arabinose inducer and an active araC gene are present, significant amounts of araBAD promoter expression take place in the ...

journal_title：FEMS microbiology letters

authors： Ben-Samoun K,Leblon G,Reyes O

更新日期：1999-05-01 00:00:00

abstract：:Saccharomyces cerevisiae was transformed with a genomic library from Penicillium purpurogenum, and an endoxylanase-producing yeast clone (named 44A) that grows on xylose or xylan as sole carbon source was isolated. This yeast synthesizes xynA mRNA and secretes endoxylanase A to culture media when grown on xylan or xyl...

journal_title：FEMS microbiology letters

authors： Chávez R,Navarro C,Calderón I,Peirano A,Bull P,Eyzaguirre J

更新日期：2002-07-02 00:00:00

abstract：:Enterococcal surface protein (Esp) is a cell wall-associated protein of Enterococcus faecalis that has been identified as a potential virulence factor. We used a mouse model to examine whether Esp facilitates intestinal colonization or translocation of E. faecalis to mesenteric lymph nodes. After clindamycin treatment...

journal_title：FEMS microbiology letters

authors： Pultz NJ,Shankar N,Baghdayan AS,Donskey CJ

更新日期：2005-01-15 00:00:00

abstract：:Crithidia oncopelti, Crithidia deanei and Crithidia desouzai are flagellates of the Trypanosomatidae family that present bacterium-like endosymbionts in their cytoplasm. Gelatin-SDS-PAGE analysis was used to characterize cell-associated and extracellular proteinases in these organisms. Our survey indicates that the pr...

journal_title：FEMS microbiology letters

authors： d'Avila-Levy CM,Melo AC,Vermelho AB,Branquinha MH

更新日期：2001-08-07 00:00:00

abstract：:Blocking glycolytic breakdown of glucose by inactivation of phosphoglucose isomerase (Pgi) in Escherichia coli led to a greatly reduced maximum specific growth rate. Examination of the operational catabolic pathways and their flux ratios using [U-(13)C(6)]glucose-labeling experiments and metabolic flux ratio analysis ...

journal_title：FEMS microbiology letters

authors： Canonaco F,Hess TA,Heri S,Wang T,Szyperski T,Sauer U

更新日期：2001-11-13 00:00:00

abstract：:In this study, internal size variations in the VanA gene cluster Tn1546, encoding resistance to glycopeptides, is described. Studies of previously uncharacterized size variations of an internal region, encoding the vanX and vanY genes of Tn1546, revealed that these variations were due to the presence of the IS sequenc...

journal_title：FEMS microbiology letters

authors： Jensen LB

更新日期：1998-12-15 00:00:00

abstract：:Bacteria carry a number of genes that cause cell growth arrest or cell lysis upon expression. Notably, defective prophages retain many lysis proteins. Here, we identified a novel lytic gene, ydfD, on the Qin prophage segment of the Escherichia coli genome. YdfD lyses 99.9% of cells within 2 h of its induction. The co-...

journal_title：FEMS microbiology letters

authors： Masuda H,Awano N,Inouye M

更新日期：2016-03-01 00:00:00

abstract：:Morphologically similar species such as Chinese black truffles and Tuber melanosporum were typed by restriction length polymorphism of internal transcribed spacers. This analysis together with sequence comparison revealed the presence of high genetic variability among fruit bodies collected in China. Selection of prim...

journal_title：FEMS microbiology letters

authors： Paolocci F,Rubini A,Granetti B,Arcioni S

更新日期：1997-08-15 00:00:00

abstract：:Abstract The emergence of the nontuberculosis mycobacteria (NTM) as clinically relevant pathogens has warranted the study of these ubiquitous organisms in the context of their likely environmental niche, the biofilm. We assayed the NTM bacterium Mycobacterium marinum strain 1218R, a fish outbreak isolate, for biofilm ...

journal_title：FEMS microbiology letters

authors： Hall-Stoodley L,Brun OS,Polshyna G,Barker LP

更新日期：2006-04-01 00:00:00

abstract：:A cyclodextrin glycosyltransferase (CGTase) gene of Brevibacillus brevis CD162 was cloned into Escherichia coli using pUC19 as a vector. Determination of the nucleotide sequence showed the presence of an open reading frame of 2079 bp encoding a polypeptide of 693 amino acid residues, composed of a 20-amino acid signal...

journal_title：FEMS microbiology letters

authors： Kim MH,Sohn CB,Oh TK

更新日期：1998-07-15 00:00:00

abstract：:Diesel fuel is a common environmental pollutant comprised of a large number of both aromatic and aliphatic hydrocarbons. The microbial degradation of individual hydrocarbons has been well characterized, however, the community dynamics within a system degrading a complex pollutant such as diesel fuel are still poorly u...

journal_title：FEMS microbiology letters

authors： Ciric L,Philp JC,Whiteley AS

更新日期：2010-02-01 00:00:00

abstract：:Corynebacterium diphtheriae strains express non-fimbrial surface proteins able to recognize and bind to specific host cells receptors. Protein extracts were obtained from bacterial cells by mechanical process and ammonium sulfate precipitation at 25 and 45% (w/v) saturation. SDS-PAGE analysis of the extracts detected ...

journal_title：FEMS microbiology letters

authors： Colombo AV,Hirata R Jr,de Souza CM,Monteiro-Leal LH,Previato JO,Formiga LC,Andrade AF,Mattos-Guaraldi AL

更新日期：2001-04-13 00:00:00

abstract：:Bacillus cereus strains involved in food poisoning cases of the diarrheal type may produce two different enterotoxin complexes. To facilitate the identification of hemolysin BL-enterotoxin complex (HBL) and/or the nonhemolytic enterotoxin (NHE) producing colonies a colony immunoblot procedure was developed, which allo...

journal_title：FEMS microbiology letters

authors： Moravek M,Wegscheider M,Schulz A,Dietrich R,Bürk C,Märtlbauer E

更新日期：2004-09-01 00:00:00

abstract：:The transport of radioactively labelled uracil into submerged mycelium of T. viride was measured by means of a membrane filtration technique. It was found to be time-dependent (up to 90 min) and concentration-dependent (up to 8 mmol l-1). Its concentration dependence was biphasic and consisted from the saturatable par...

journal_title：FEMS microbiology letters

authors： Lakatos B,Simkovic M,Betina V,Varecka L

更新日期：1999-02-15 00:00:00

abstract：:Macrolide is inactivated with ATP plus crude extract of Escherichia coli producing macrolide 2'-phosphotransferase (MPH(2')), but not by living cells. Therefore, a convenient method for detection of MPH(2') using intact cells is needed. In this report, we determine that the modified lysozyme-DNase-RNase (LDR) method (...

journal_title：FEMS microbiology letters

authors： Taniguchi K,Nakamura A,Tsurubuchi K,O'Hara K,Sawai T

更新日期：1998-10-15 00:00:00

abstract：:Peptidoglycan hydrolases are an effective new source of antimicrobials. A chimeric fusion protein of the Ply187 endopeptidase domain and LysK SH3b cell wall-binding domain is a potent agent against Staphylococcus aureus in four functional assays. ...

journal_title：FEMS microbiology letters

authors： Mao J,Schmelcher M,Harty WJ,Foster-Frey J,Donovan DM

更新日期：2013-05-01 00:00:00

abstract：:Herein, we report a high-quality draft genome sequence of an uncultivated aromatic compound-degrading bacterium, obtained by the stable isotope probing method from a sulfate-reducing microcosm from an oil-contaminated tidal flat. The obtained genome was closely related with that of Desulfobacula toluolica Tol2. Abunda...

journal_title：FEMS microbiology letters

authors： Kim SJ,Park SJ,Jung MY,Kim JG,Min UG,Hong HJ,Rhee SK

更新日期：2014-11-01 00:00:00

abstract：:Methanobacterium barkeri was found to catalyze the reductive dehalogenation of trichlorofluoromethane (CFC-11), also known as FREON 11. Products detected were CHFCl2, CH2FCl, CO and fluoride. ...

journal_title：FEMS microbiology letters

authors： Krone UE,Thauer RK

更新日期：1992-01-01 00:00:00

abstract：:A Na(+)/H(+) antiporter gene was isolated from alkaliphilic Bacillus sp. G1. The full-length sequence of the Na(+)/H(+) antiporter gene was obtained using a genome walking method, and designated as g1-nhaC. An ORF preceded by a promoter-like sequence and a Shine-Dalgarno sequence, and followed by a terminator-like seq...

journal_title：FEMS microbiology letters

authors： Liew CW,Illias RM,Mahadi NM,Najimudin N

更新日期：2007-11-01 00:00:00

abstract：:In the industrialized world, functional foods have become a part of an everyday diet and are demonstrated to offer potential health benefits beyond the widely accepted nutritional effects. Currently, the most important and frequently used functional food compounds are probiotics and prebiotics, or they are collectivel...

journal_title：FEMS microbiology letters

authors： Nagpal R,Kumar A,Kumar M,Behare PV,Jain S,Yadav H

更新日期：2012-09-01 00:00:00

abstract：:The glnAntrBC operon of Proteus vulgaris was cloned and heterologously expressed in Escherichia coli. The nucleotide sequence was determined. An open reading frame of 1407 bp was identified as the glnA gene and the deduced amino acid sequence showed 82% identity with the E. coli glutamine synthetase protein. Heterolog...

journal_title：FEMS microbiology letters

authors： Steglitz-Mörsdorf U,Mörsdorf G,Kaltwasser H

更新日期：1993-01-15 00:00:00

abstract：:Sixty-four breast-fed infants and 23 calves were investigated for bacteria and enzymatic activity in their faecal samples. The bacteria were measured using cultivation and fluorescence in situ hybridization. Enzymatic activity was also examined. Forty-seven (64%) infants and all the calves had high numbers of bifidoba...

journal_title：FEMS microbiology letters

authors： Rada V,Vlková E,Nevoral J,Trojanová I

更新日期：2006-05-01 00:00:00