Abstract:
:The genus Burkholderia comprises over 28 species and species-specific, recA-based polymerase chain reaction (PCR) tests are available for several species, but not for some soil-inhabiting species including B. fungorum. Previous analysis of several novel rhizospheric, environmental isolates belonging to the B. cepacia complex suggested they may be closely related to B. fungorum. To discover any relationship between these isolates and B. fungorum we set out to clone and sequence a portion of the B. fungorum recA gene in order to design species-specific primer pairs for use in a recA-based PCR assay. Using a similar procedure we extended the recA-based PCR assay to identify B. sacchari and B. caledonica, two additional soil-inhabiting Burkholderia spp.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Chan CH,Stead DE,Coutts RHdoi
10.1016/S0378-1097(03)00443-9keywords:
subject
Has Abstractpub_date
2003-07-15 00:00:00pages
133-8issue
1eissn
0378-1097issn
1574-6968pii
S0378109703004439journal_volume
224pub_type
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