Abstract:
:A semi-automated method has been developed for the quantification and measurement of conidia discharged by the aphid pathogen Erynia neoaphidis. This was used to compare conidiation by E. neoaphidis-mycosed pea aphid cadavers, mycelial plugs cut from agar plates, mycelial pellets from shake flasks and by mycelial pellets from different phases of liquid batch fermenter culture. Aphid cadavers discharged significantly more and significantly smaller conidia than plugs or pellets. The volume of conidia discharged was stable over the period of discharge (80 h), but more detailed analysis of the size frequency distribution showed that more very small and very large conidia were discharged after 5 h incubation than after 75 h incubation. Biomass harvested at the end of the exponential growth phase in batch fermenter culture produced significantly more conidia than biomass from any other growth phase. The implications of these findings for the development of production and formulation processes for E. neoaphidis as a biological control agent are discussed.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Bonner TJ,Pell JK,Gray SNdoi
10.1016/S0378-1097(03)00063-6keywords:
subject
Has Abstractpub_date
2003-03-14 00:00:00pages
75-80issue
1eissn
0378-1097issn
1574-6968pii
S0378109703000636journal_volume
220pub_type
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