Denaturing gradient gel electrophoresis gel expansion (DGGEGE)--an attempt to resolve the limitations of co-migration in the DGGE of complex polymicrobial communities.

abstract：:DNA polymerase from the archaeon Pyrococcus abyssi strain Orsay was expressed in Escherichia coli. The recombinant DNA polymerase (Pab) was purified to homogeneity by heat treatment followed by 5 steps of chromatography and characterized for PCR applications. Buffer optimization experiments indicated that Pab PCR perf...

journal_title：FEMS microbiology letters

authors： Dietrich J,Schmitt P,Zieger M,Preve B,Rolland JL,Chaabihi H,Gueguen Y

更新日期：2002-11-19 00:00:00

abstract：:We have studied the intraperoxisomal location of catalase in peroxisomes of methanol-grown Hansenula polymorpha by (immuno)cytochemical means. In completely crystalline peroxisomes, in which the crystalline matrix is composed of octameric alcohol oxidase (AO) molecules, most of the catalase protein is located in a nar...

journal_title：FEMS microbiology letters

authors： Keizer I,Roggenkamp R,Harder W,Veenhuis M

更新日期：1992-05-15 00:00:00

abstract：:Bacillus cereus is becoming one of the more important causes of food poisoning in the industrialised world. It produces one emetic toxin and three different enterotoxins. The emetic toxin is a ring-shaped structure of three repeats of four amino and/or oxy acids: [D-O-Leu-D-Ala-L-O-Val-L-Val]3. This ring structure has...

journal_title：FEMS microbiology letters

authors： Granum PE,Lund T

更新日期：1997-12-15 00:00:00

abstract：:The structure and distribution of a Mycobacterium bovis BCG insertion element of the IS21 family were investigated. Several IS21-like elements found in mycobacterial genomes were separated in four types, following their nucleic acid similarities. The M. bovis BCG IS21 element is highly similar to IS1533 (class I), 70%...

journal_title：FEMS microbiology letters

authors： Lefèvre P,Braibant M,Content J,Gilot P

更新日期：1999-09-15 00:00:00

abstract：:Marine viruses are the most abundant biological entity in the oceans, the majority of which infect bacteria and are known as bacteriophages. Yet, the bulk of bacteriophages form part of the vast uncultured dark matter of the microbial biosphere. In spite of the paucity of cultured marine bacteriophages, it is known th...

journal_title：FEMS microbiology letters

authors： Perez Sepulveda B,Redgwell T,Rihtman B,Pitt F,Scanlan DJ,Millard A

更新日期：2016-08-01 00:00:00

abstract：:5-Carboxymethyl-2-hydroxymuconic semialdehyde (CHMS) dehydrogenase from Escherichia coli C and Klebsiella pneumoniae M5a1 have been purified and some of their properties studied. The apparent Km values for NAD and CHMS were 11.7 +/- 1.5 microM and 5.2 +/- 1.9 microM, respectively, for the K. pneumoniae enzyme, and 19....

journal_title：FEMS microbiology letters

authors： Fawcett T,Garrido-Pertierra A,Cooper RA

更新日期：1989-02-01 00:00:00

abstract：:Environmental plasmids often expand the metabolic repertoire of bacteria that carry them, but they also interfere with the biochemical and genetic network of the host. The pWW0 plasmid born by Pseudomonas putida mt-2 encodes the TOL pathway for degradation of toluene/m-xylene through production of intermediate compoun...

journal_title：FEMS microbiology letters

authors： Silva-Rocha R,de Lorenzo V

更新日期：2014-07-01 00:00:00

abstract：:The bft gene encoding Bacteroides fragilis toxin (BFT) has been devided into two subtypes, bft-1 and bft-2. We found a novel subtype by sequencing a segment of the bft gene from 64 enterotoxigenic B. fragilis (ETBF) strains isolated in Japan. The 1548-bp nucleotide sequences of the new bft, the bft-1, and bft-2 genes ...

journal_title：FEMS microbiology letters

authors： Kato N,Liu CX,Kato H,Watanabe K,Tanaka Y,Yamamoto T,Suzuki K,Ueno K

更新日期：2000-01-01 00:00:00

abstract：:Burkholderia pseudomallei, a Gram-negative bacterium is found in the soil and water, mainly in Southeast Asia and Northern Australia. It is responsible for melioidosis in human and animals. The bacteria produce several potential virulent factors such as extracellular protease, hemolysin, lipase and lecithinase. The is...

journal_title：FEMS microbiology letters

authors： Lee MA,Liu Y

更新日期：2000-11-01 00:00:00

abstract：:Two actinomycete strains, CHR3 and CHR28, were isolated from metal-contaminated sediments from Baltimore Inner Harbor. The isolates were classified as Streptomyces spp. Agar diffusion assays showed both isolates to be resistant to mercuric chloride and phenylmercuric acetate. Hybridization experiments indicated that g...

journal_title：FEMS microbiology letters

authors： Ravel J,Amoroso MJ,Colwell RR,Hill RT

更新日期：1998-05-01 00:00:00

abstract：:The physiological role of mitochondrial aldehyde dehydrogenase (ALD5) was investigated by analysis of the ald5 mutant (AKD321) in Saccharomyces cerevisiae. K(+)-activated ALDH activity of the ald5 mutant was about 80% of the wild-type in the mitochondrial fraction, while the respiratory activity of the ald5 mutant was...

journal_title：FEMS microbiology letters

authors： Kurita O,Nishida Y

更新日期：1999-12-15 00:00:00

abstract：:Bacterial swarming motility is a flagella-dependent translocation on the surface environment. It has received extensive attention as a population behavior involving numerous genes. Here, we report that Citrobacter freundii, an opportunistic pathogen, exhibits swarming movement on a solid medium surface with appropriat...

journal_title：FEMS microbiology letters

authors： Cong Y,Wang J,Chen Z,Xiong K,Xu Q,Hu F

更新日期：2011-04-01 00:00:00

abstract：:Acinetobacter baumanii, which may be found in water, is an important emerging hospital-acquired pathogen. Free-living amoebae can be recovered from the same water networks, and it has been shown that these protozoa may support the growth of other bacteria. In this paper, we have studied potential relationships between...

journal_title：FEMS microbiology letters

authors： Cateau E,Verdon J,Fernandez B,Hechard Y,Rodier MH

更新日期：2011-06-01 00:00:00

abstract：:The diheme cytochromes c of the widespread TsdA family are bifunctional thiosulfate dehydrogenase/tetrathionate reductases. Here, biochemical information was collected about TsdA from the Epsilonproteobacterium Wolinella succinogenes (WsTsdA). The situation in W. succinogenes is unique since TsdA is closely associated...

journal_title：FEMS microbiology letters

authors： Kurth JM,Schuster A,Seel W,Herresthal S,Simon J,Dahl C

更新日期：2017-02-01 00:00:00

abstract：:The time course production of xylanolytic enzymes by the rumen anaerobic fungus Neocallimastix frontalis was studied during growth on different carbon sources and revealed using isoelectric focusing and immunoblotting. A constant low level of endoxylanase expression was observed in glucose medium. A high level of xyla...

journal_title：FEMS microbiology letters

authors： Gomez de Segura B,Durand R,Fèvre M

更新日期：1998-07-01 00:00:00

abstract：:Isolates of anaerobic fungi obtained from the rumen, duodenum and faeces of sheep were identified as Piromyces mae based on their morphological characteristics observed using light microscopy. There was no significant morphological variation among the isolates of P. mae from the rumen, duodenum and faeces. Isozymes of...

journal_title：FEMS microbiology letters

authors： Wong MV,Ho YW,Tan SG,Abdullah N,Jalaludin S

更新日期：1995-12-01 00:00:00

abstract：:Streptococcus suis serotype 2 (S. suis 2 or SS2) is the causative agent of several diseases in both pigs and humans. 6-Phosphogluconate dehydrogenase (6PGD) is a cell surface protein in SS2. In this study, the immunogenicity and protective efficacy of recombinant 6PGD (r6PGD) from SS2 was evaluated in piglets. Immuniz...

journal_title：FEMS microbiology letters

authors： Tan C,Liu M,Liu J,Yuan F,Fu S,Liu Y,Jin M,Bei W,Chen H

更新日期：2009-07-01 00:00:00

abstract：:Brucella melitensis possesses an operon with two components: the response regulator OtpR and a putative cAMP-dependent protein kinase regulatory subunit encoded by the BMEI0067 gene. In the previous study, the function of OtpR has been studied, while little is known about the function of the BMEI0067 gene. Using a bio...

journal_title：FEMS microbiology letters

authors： Liu WJ,Dong H,Peng XW,Wu QM

更新日期：2014-07-01 00:00:00

abstract：:Binding of meningitis-causing Escherichia coli K1 to human brain microvascular endothelial cells (HBMEC) contributes to traversal of the blood-brain barrier, which occurs in part by the mannose-sensitive binding of FimH. In this study, we showed that FimH also binds to HBMEC, independent of mannose, and identified ATP...

journal_title：FEMS microbiology letters

authors： Shin S,Kim KS

更新日期：2010-02-01 00:00:00

abstract：:The pseudotrisaccharide allosamidin, a potent inhibitor of chitinases, retarded the fragmentation of hyphae but did not affect the fungal growth and cephalosporin-C production in Acremonium chrysogenum. In vitro inhibition of A. chrysogenum cell-bound chitinase(s) by allosamidin revealed that about 47% of the soluble ...

journal_title：FEMS microbiology letters

authors： Sándor E,Pusztahelyi T,Karaffa L,Karányi Z,Pócsi I,Biró S,Szentirmai A,Pócsi I

更新日期：1998-07-15 00:00:00

abstract：:We developed an improved HEp-2 cell assay method for the detection of Bacillus cereus toxin, which affects the proliferation of HEp-2 cells. The cytostatic toxin was stable upon exposure to heat, pH 2, pH 11 and trypsin, which suggests it is an emetic. Using the HEp-2 cell assay, we examined the distribution and conta...

journal_title：FEMS microbiology letters

authors： Mikami T,Horikawa T,Murakami T,Matsumoto T,Yamakawa A,Murayama S,Katagiri S,Shinagawa K,Suzuki M

更新日期：1994-06-01 00:00:00

abstract：:We have constructed a Thermus promoter probe vector pPP11 containing the promoterless heat-stable kanamycin resistance (Kmr) gene. Total DNA of T. thermophilus HB27 was randomly sheared into 100-200 bp fragments and inserted into the EcoRI site of pPP11. About 1000 clones which showed Kmr were obtained, and they were ...

journal_title：FEMS microbiology letters

authors： Maseda H,Hoshino T

更新日期：1995-05-01 00:00:00

abstract：:Growth of Pseudomonas aeruginosa on acyclic terpene alcohols (citronellol) and on other methyl-branched compounds such as leucine or isovalerate requires a functional leucine/isovalerate utilization (Liu) pathway. In this study, we investigated the liuABCDE gene cluster by insertion mutant analysis, heterologous expre...

journal_title：FEMS microbiology letters

authors： Förster-Fromme K,Jendrossek D

更新日期：2008-09-01 00:00:00

abstract：:The filamentous fungus Cunninghamella elegans has the ability to metabolize xenobiotics, including polycyclic aromatic hydrocarbons and pharmaceutical drugs, by both phase I and II biotransformations. Cytosolic and microsomal fractions were assayed for activities of cytochrome P450 monooxygenase, aryl sulfotransferase...

journal_title：FEMS microbiology letters

authors： Zhang D,Yang Y,Leakey JE,Cerniglia CE

更新日期：1996-05-01 00:00:00

abstract：:Hydrogenobacter acidophilus strain 3H-1 is a thermoacidophilic, obligately chemolithoautotrophic, hydrogen-oxidizer isolated from a Japanese solfataric field. Strain 3H-1 requires elemental sulfur for growth. We used PCR to amplify the 16S rRNA gene of strain 3H-1, and sequenced the amplification product directly. Phy...

journal_title：FEMS microbiology letters

authors： Shima S,Yanagi M,Saiki H

更新日期：1994-06-01 00:00:00

abstract：:This review describes the factors which are currently recognized as being central to the virulence of the human pathogen, Vibrio vulnificus. This estuarine/marine bacterium occurs in high numbers in molluscan shellfish, primarily oysters, and its ingestion in raw oysters results in a ca. 60% mortality in those persons...

journal_title：FEMS microbiology letters

authors： Linkous DA,Oliver JD

更新日期：1999-05-15 00:00:00

abstract：:A serum-susceptible, guinea-pig chamber-passaged, laboratory strain (BS4 (agar)) of Neisseria gonorrhoeae was converted to serum resistance by incubation with cytidine 5-monophospho-N-acetyl neuraminic acid (CMP-NANA) and examined by electron microscopy after staining with ruthenium-red. In contrast to serum susceptib...

journal_title：FEMS microbiology letters

authors： Fox AJ,Curry A,Rowland PL,Lancaster S,Jones DM,Parsons NJ,Cole JA,Smith H

更新日期：1990-01-01 00:00:00

abstract：:Falcipain-2A, the cysteine protease of Plasmodium falciparum has been proposed as a good drug target. This study evaluated the suitability of Plasmodium berghei as the animal model and reports the first functional expression and characterization of the falcipain-2A orthologue, berghepain-2. Comparative studies reveale...

journal_title：FEMS microbiology letters

authors： Chan C,Goh LL,Sim TS

更新日期：2005-08-15 00:00:00

abstract：:The central carbon metabolism is well investigated in bacteria, but this is not the case for archaea. MJ0400-His(6) from Methanocaldococcus jannaschii catalyzes the cleavage of fructose-1,6-bisphosphate (FBP) to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate with a V(max) of 33 mU mg(-1) and a K(m) of 430 m...

journal_title：FEMS microbiology letters

authors： Samland AK,Wang M,Sprenger GA

更新日期：2008-04-01 00:00:00

abstract：:Pseudomonas aeruginosa are known to have a wide physiological potential allowing them to constantly populate diverse environments leading to severe infections of humans such as septicemia, leg ulcers, and burn wounds. We set out to probe physiological characteristics of P. aeruginosa isolates from diabetic leg ulcers ...

journal_title：FEMS microbiology letters

authors： Buivydas A,Pasanen T,Senčilo A,Daugelavičius R,Vaara M,Bamford DH

更新日期：2013-06-01 00:00:00