Characterization of a Mycobacterium bovis BCG insertion sequence related to the IS21 family.

abstract：:Helicobacter pylori genomes contain about 30 hop genes that encode outer membrane proteins. Helicobacter pylori hopQ alleles exhibit a high level of genetic diversity, and two families of hopQ alleles have been described. Type I hopQ alleles are found more commonly in cag-positive H. pylori strains from patients with ...

journal_title：FEMS microbiology letters

authors： Loh JT,Torres VJ,Algood HM,McClain MS,Cover TL

更新日期：2008-12-01 00:00:00

abstract：:Serratia entomophila and Serratia proteamaculans cause amber disease of the grass grub Costelytra zealandica (Coleoptera: Scarabaeidae). Three genes required for virulence, sepABC, are located on a large plasmid, pADAP. The translated products of the sep genes are members of the toxin complex (Tc) family of insecticid...

journal_title：FEMS microbiology letters

authors： Hurst MR,Jones SM,Tan B,Jackson TA

更新日期：2007-10-01 00:00:00

abstract：:The ADP-ribosylation factor (ARF) family of GTPases are highly conserved from yeast to human and regulate vesicle budding. Sec7 domain containing proteins stimulate the guanine nucleotide exchange on Arf proteins, while ARF-GTPase activating proteins stimulate the hydrolysis of GTP. Since vesicle trafficking is import...

journal_title：FEMS microbiology letters

authors： Oscarsson T,Walther A,Lengeler KB,Wendland J

更新日期：2017-12-29 00:00:00

abstract：:An open reading frame located in the tyrB-uvrA intergenic region of the Escherichia coli MG1655 chromosome was identified as encoding the class B acid phosphatase of this species on the basis of cloning and expression experiments. A protocol for purification of the enzyme (named AphA) was developed, and its properties...

journal_title：FEMS microbiology letters

authors： Thaller MC,Schippa S,Bonci A,Cresti S,Rossolini GM

更新日期：1997-01-15 00:00:00

abstract：:We have investigated the mechanisms used by Trichomonas vaginalis to damage cellular membranes, using human erythrocytes as target cells. Haemolysis is a contact- and temperature-dependent phenomenon, and is inhibited in 4 mM EGTA. Osmotic protection experiments using carbohydrates with different molecular diameters a...

journal_title：FEMS microbiology letters

authors： Fiori PL,Rappelli P,Rocchigiani AM,Cappuccinelli P

更新日期：1993-05-01 00:00:00

abstract：:A cyclodextrin glycosyltransferase (CGTase) gene of Brevibacillus brevis CD162 was cloned into Escherichia coli using pUC19 as a vector. Determination of the nucleotide sequence showed the presence of an open reading frame of 2079 bp encoding a polypeptide of 693 amino acid residues, composed of a 20-amino acid signal...

journal_title：FEMS microbiology letters

authors： Kim MH,Sohn CB,Oh TK

更新日期：1998-07-15 00:00:00

abstract：:Intracellular copper routing in Enterococcus hirae is accomplished by the CopZ copper chaperone. Under copper stress, CopZ donates Cu(+) to the CopY repressor, thereby releasing its bound zinc and abolishing repressor-DNA interaction. This in turn induces the expression of the cop operon, which encodes CopY and CopZ, ...

journal_title：FEMS microbiology letters

authors： Stoyanov JV,Mancini S,Lu ZH,Mourlane F,Poulsen KR,Wimmer R,Solioz M

更新日期：2010-01-01 00:00:00

abstract：:The nucleotide sequence of a 714-bp DNA fragment containing the enterotoxic Escherichia coli (ETEC) adhesive factor 8786 structural gene, designated nfaA, revealed an open reading frame of 498 bp encoding a polypeptide of 166 amino acids. Primer-extension experiments showed that the nfaA gene is within a single transc...

journal_title：FEMS microbiology letters

authors： Aubel D,Darfeuille-Michaud A,Martin C,Joly B

更新日期：1992-11-01 00:00:00

abstract：:The frequency of spontaneous as well as induced reversions of auxotrophic mutations in Escherichia coli AB1157 and its DeltadnaK and DeltadnaKdnaJ derivatives was estimated. The obtained results demonstrate that both mutants tested are characterized by elevated frequency of spontaneous reversions compared to their AB1...

journal_title：FEMS microbiology letters

authors： Grudniak AM,Kuć M,Wolska KI

更新日期：2005-01-15 00:00:00

abstract：:An autonomous impala transposon trapped in Fusarium oxysporum by insertion within the niaD gene encoding nitrate reductase was introduced in the genome of the fungus Penicillium griseoroseum, a producer of pectinase enzymes. Through a phenotypic assay, we demonstrate that this element is able to excise from the niaD g...

journal_title：FEMS microbiology letters

authors： de Queiroz MV,Daboussi MJ

更新日期：2003-01-28 00:00:00

abstract：:Many bacteria are capable of accumulating intracellular granules of polyhydroxyalkanoates (PHA). In this work, we developed confocal microscopy analysis of bacterial cells to study changes in the diameters of cells as well as PHA granules during growth and PHA accumulation in the bacterium Cupriavidus necator H16 (for...

journal_title：FEMS microbiology letters

authors： Mravec F,Obruca S,Krzyzanek V,Sedlacek P,Hrubanova K,Samek O,Kucera D,Benesova P,Nebesarova J

更新日期：2016-05-01 00:00:00

abstract：:This study was initiated to establish whether inhibition of growth of yeasts by medium-chain fatty acids resembled that caused by weak-acid preservatives or uncouplers. Unlike sorbic acid and 2,4-dinitrophenol, decanoic acid caused rapid cell death at its inhibitory concentration. This suggested a mode of action by me...

journal_title：FEMS microbiology letters

authors： Stratford M,Anslow PA

更新日期：1996-08-15 00:00:00

abstract：:In this multidisciplinary study, we combined morphological, physiological, and phylogenetic approaches to identify three dominant water bloom-forming Cyanobacteria in a tropical marine mangrove in Guadeloupe (French West Indies). Phylogenetic analysis based on 16S rRNA gene sequences place these marine Cyanobacteria i...

journal_title：FEMS microbiology letters

authors： Guidi-Rontani C,Jean MR,Gonzalez-Rizzo S,Bolte-Kluge S,Gros O

更新日期：2014-10-01 00:00:00

abstract：:Immunoassays are increasingly used to investigate the production, properties and fates of the cyanobacterial hepatotoxic microcystins in vitro and in vivo. Responses of an ELISA immunoassay to microcystins have been determined using the authentic toxin antigen, microcystin-LR, and conjugation products between the toxi...

journal_title：FEMS microbiology letters

authors： Metcalf JS,Beattie KA,Pflugmacher S,Codd GA

更新日期：2000-08-15 00:00:00

abstract：:A search of the complete genome sequence of the halophilic archaeon Halobacterium salinarum failed to identify a gene homologous to the thymidylate synthase (thyA) gene present in the closely related Haloferax volcanii. To understand the source of thymidine synthesis in Hbt. salinarum, a genomic library of Hbt. salina...

journal_title：FEMS microbiology letters

authors： Giladi M,Bitan-Banin G,Mevarech M,Ortenberg R

更新日期：2002-10-29 00:00:00

abstract：:We have developed a novel type of a positive screen for the discovery of antibacterial compounds that target the Escherichia coli replication initiator protein DnaA. DnaA is an essential replication protein, conserved in (almost) all bacteria--including all human pathogens--and no existing antibiotics target the main ...

journal_title：FEMS microbiology letters

authors： Fossum S,De Pascale G,Weigel C,Messer W,Donadio S,Skarstad K

更新日期：2008-04-01 00:00:00

abstract：:The miniplasmid profiles of 18 Bacillus thuringiensis strains belonging to 8 different serotypes were determined using an alkaline hydrolysis method for isolation of low molecular mass plasmids. Nearly all the strains contained covalently closed circular (CCC) DNA species ranging from 2 to 5 species per strain and fro...

journal_title：FEMS microbiology letters

authors： Marin R,Tanguay RM,Valéro J,Letarte R,Bellemare G

更新日期：1992-07-15 00:00:00

abstract：:It was shown that virulent Mycobacterium tuberculosis H37Rv induces necrosis of infected RAW264 cells at 24 h post infection while avirulent H37Ra and an attenuated H37Rv mutant that is deficient for RD1 region (H37RvDeltaRD1) cause less necrosis of the infected cells. While H37Rv caused damage of the mitochondrial in...

journal_title：FEMS microbiology letters

authors： Kaku T,Kawamura I,Uchiyama R,Kurenuma T,Mitsuyama M

更新日期：2007-09-01 00:00:00

abstract：:A Tn5 transposon mutant was isolated of the alkylphenol degrader Pseudomonas sp. KL28 that showed increased growth at higher levels of m-cresol on solid and in liquid cultures. The transposon was inserted at the 5'-terminus of rpoS, which encodes a stationary-phase sigma factor. When grown on agar plates, the rpoS mut...

journal_title：FEMS microbiology letters

authors： Yun JI,Cho KM,Kim JK,Lee SO,Cho K,Lee K

更新日期：2007-04-01 00:00:00

abstract：:A two-step enzymatic synthesis process of 4-hydroxyisoleucine is suggested. In the first step, the aldol condensation of acetaldehyde and alpha-ketobutyrate catalyzed by specific aldolase results in the formation of 4-hydroxy-3-methyl-2-keto-pentanoate (HMKP). In the second step, amination of HMKP by the branched-chai...

journal_title：FEMS microbiology letters

authors： Smirnov SV,Samsonova NN,Novikova AE,Matrosov NG,Rushkevich NY,Kodera T,Ogawa J,Yamanaka H,Shimizu S

更新日期：2007-08-01 00:00:00

abstract：:The 0104 antigen (lipopolysaccharide, LPS) of Escherichia coli has an acidic O specific polysaccharide. From the aqueous phase of a phenol water extraction of E. coli O104: K-, a fraction was obtained by ultracentrifugation and Cetavlon precipitation of the supernatant, which was enriched in long-chain LPS. Compositio...

journal_title：FEMS microbiology letters

authors： Kogan G,Jann B,Jann K

更新日期：1992-03-01 00:00:00

abstract：:Alcaligenes sp. strain PPH degrades phenanthrene via 1-hydroxy-2-naphthoic acid (1-H2NA), 1,2-dihydroxynaphthalene (1,2-DHN), salicylic acid and catechol. Enzyme activity versus growth profile and heat stability studies suggested the presence of two distinct hydroxylases, namely 1-hydroxy-2-naphthoic acid hydroxylase ...

journal_title：FEMS microbiology letters

authors： Deveryshetty J,Phale PS

更新日期：2010-10-01 00:00:00

abstract：:When the amino acid-fermenting bacterium Clostridium aminophilum F was inoculated into media containing 1 microM monensin or a bacteriocin-like inhibitory substance (BLIS) from Butyrivibrio fibrisolvens JL5, the cultures lagged and growth was not observed for more than 12 h. The monensin- and BLIS-treated cultures eve...

journal_title：FEMS microbiology letters

authors： Rychlik JL,Russell JB

更新日期：2002-03-19 00:00:00

abstract：:Aspergillus niger represents a promising host for the expression of recombinant proteins, but only a few expression systems are available for this organism. In this study, the inducible catalase promoter (PcatR) from A. niger was characterized. For this, constructs were developed and checked for the expression of the ...

journal_title：FEMS microbiology letters

authors： Sharma R,Katoch M,Govindappa N,Srivastava PS,Sastry KN,Qazi GN

更新日期：2012-02-01 00:00:00

abstract：:To investigate the regulatory mechanism governing antifungal metabolite biosynthesis, two kinds of global regulator genes in Pseudomonas sp. M18, an rpoS and an rsmA gene, were cloned and mutated by inserting with an aacC1 cassette, respectively. Two mutants showed the same regulatory mode with repression of phenazine...

journal_title：FEMS microbiology letters

authors： Ge Y,Yang S,Fang Y,Yang R,Mou D,Cui J,Wen L

更新日期：2007-03-01 00:00:00

abstract：:To clarify the cause of the supersusceptibility of Branhamella catarrhalis to macrolide antibiotics, which are well-known to be inactive to most Gram-negative bacteria, we determined its cell surface hydrophobicity by the partition experiment between water and hydrocarbons. Its cell surface was found to be markedly mo...

journal_title：FEMS microbiology letters

authors： Gotoh N,Tanaka S,Nishino T

更新日期：1989-05-01 00:00:00

abstract：:The purpose of this study was to generate highly specific serological reagents for the quantitative identification of Actinomyces naeslundii in clinical samples, in particular dental plaque. Balb/c mice were immunized with pasteurized human A. naeslundii strains representing different genospecies and serotypes. Ten hy...

journal_title：FEMS microbiology letters

authors： Thurnheer T,Guggenheim B,Gmür R

更新日期：1997-05-15 00:00:00

abstract：:D-(-)-3-Hydroxybutyrate (3HB) oligomer hydrolase was purified from Paracoccus denitrificans. The enzyme was a monomeric protein with an approximate molecular mass of 31 kDa. The isoelectric point of the enzyme was 5.2. Optimum temperature and pH were 35-40 degrees C and 8.0, respectively. The enzyme activity was not a...

journal_title：FEMS microbiology letters

authors： Ueda S,Sano K,Gao D,Tomihari N,Yamane T,Endo I

更新日期：2002-01-10 00:00:00

abstract：:In spite of being dispensable, O-antigens are believed to facilitate various cellular processes and alter antibiotic sensitivities. Escherichia coli K-12 (CS109) strains are lacking in O-antigens and are reported to be sensitive to antibiotics. To our surprise, E. coli 2443 (expressing O8-antigen) manifested two- to f...

journal_title：FEMS microbiology letters

authors： Sarkar SK,Ghosh AS

更新日期：2008-05-01 00:00:00

abstract：:Exopolysaccharide (EPS) was prepared by submerged mycelial culture of a newly isolated mushroom Grifola frondosa HB0071 in a 5-l stirred-tank fermenter. This fungus produced a high concentration of biomass (24.8 gl(-1) at day 4), thereby achieving high EPS concentration (7.2 gl(-1) at day 4). EPS was proven to be a pr...

journal_title：FEMS microbiology letters

authors： Bae JT,Sim GS,Lee DH,Lee BC,Pyo HB,Choe TB,Yun JW

更新日期：2005-10-15 00:00:00