Multiplicity and expression of xylanases in the rumen fungus Neocallimastix frontalis.

Abstract:

:The time course production of xylanolytic enzymes by the rumen anaerobic fungus Neocallimastix frontalis was studied during growth on different carbon sources and revealed using isoelectric focusing and immunoblotting. A constant low level of endoxylanase expression was observed in glucose medium. A high level of xylanase activity was detected in methyl glucoside medium corresponding to the induction of new isoforms which were repressed by the presence of glucose. beta-Xylosidases were constitutively produced at a high level and remained mainly associated to the fungal cells. Polyclonal antibodies raised against the endoxylanases XYLI and XYLII revealed that XYLI was secreted to the different culture media showing a characteristic pattern of constitutive expression, while anti-XYLII recognized several polypeptides larger than XYLII indicating the production of multiple antigenically related enzymes during growth on the inducing substrate.

journal_name

FEMS Microbiol Lett

authors

Gomez de Segura B,Durand R,Fèvre M

doi

10.1111/j.1574-6968.1998.tb13066.x

subject

Has Abstract

pub_date

1998-07-01 00:00:00

pages

47-53

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(98)00190-6

journal_volume

164

pub_type

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