Abstract:
:The time course production of xylanolytic enzymes by the rumen anaerobic fungus Neocallimastix frontalis was studied during growth on different carbon sources and revealed using isoelectric focusing and immunoblotting. A constant low level of endoxylanase expression was observed in glucose medium. A high level of xylanase activity was detected in methyl glucoside medium corresponding to the induction of new isoforms which were repressed by the presence of glucose. beta-Xylosidases were constitutively produced at a high level and remained mainly associated to the fungal cells. Polyclonal antibodies raised against the endoxylanases XYLI and XYLII revealed that XYLI was secreted to the different culture media showing a characteristic pattern of constitutive expression, while anti-XYLII recognized several polypeptides larger than XYLII indicating the production of multiple antigenically related enzymes during growth on the inducing substrate.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Gomez de Segura B,Durand R,Fèvre Mdoi
10.1111/j.1574-6968.1998.tb13066.xsubject
Has Abstractpub_date
1998-07-01 00:00:00pages
47-53issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(98)00190-6journal_volume
164pub_type
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