Purification and properties of a 1,3-beta-glucanase from Penicillium oxalicum autolysates.

abstract：:Different genes encoding pyridine nucleotide transhydrogenases (pntAB, udhA) and acetoacetyl-CoA reductases (phaB) were heterologously overexpressed in Rhodospirillum rubrum S1. A recombinant strain, which harbored the gene encoding the membrane-bound transhydrogenase PntAB from Escherichia coli MG1655 and the phaB1 g...

journal_title：FEMS microbiology letters

authors： Heinrich D,Raberg M,Steinbüchel A

更新日期：2015-04-01 00:00:00

abstract：:The genus Xanthomonas is composed of several species that cause severe crop losses around the world. In Latin America, one of the most relevant species is Xanthomonas campestris pv. campestris, which is responsible for black rot in cruciferous plants. This pathogen causes yield losses in several cultures, including ca...

journal_title：FEMS microbiology letters

authors： Andrade AE,Silva LP,Pereira JL,Noronha EF,Reis FB Jr,Bloch C Jr,dos Santos MF,Domont GB,Franco OL,Mehta A

更新日期：2008-04-01 00:00:00

abstract：:Fungi of the genus Fusarium are important plant pathogens and contaminants of cereal grains producing different types of mycotoxins. Enniatins are a group of mycotoxins with ionophoric properties frequently detected in North European grains. Within the Fusarium complex responsible for grain infection, Fusarium avenace...

journal_title：FEMS microbiology letters

authors： Kulik T,Jestoi M,Okorski A

更新日期：2011-01-01 00:00:00

abstract：:Pneumocystis carinii pneumonia was induced in rats by chronic corticosteroid immunosuppression. The parasite was separated from virtually all contaminating host cells using the technique of unit gravity sedimentation. Cellular contamination was routinely below 0.02%. The same technique allowed partial separation of th...

journal_title：FEMS microbiology letters

authors： Taylor MB,Easmon CS

更新日期：1990-06-15 00:00:00

abstract：:Trichoderma reesei is a filamentous fungus widely used as an efficient protein producer and known to secrete large quantities of biomass degrading enzymes. Much work has been done aimed at improving the secretion efficiency of this fungus. It is generally accepted that the major bottlenecks in secretion are protein fo...

journal_title：FEMS microbiology letters

authors： Diener SE,Dunn-Coleman N,Foreman P,Houfek TD,Teunissen PJ,van Solingen P,Dankmeyer L,Mitchell TK,Ward M,Dean RA

更新日期：2004-01-30 00:00:00

abstract：:Lactobacilli have been shown to be important in the maintenance of the healthy urogenital flora. One strain, Lactobacillus fermentum RC-14, releases surface-active components which can inhibit adhesion of uropathogenic bacteria. Using a quantitative method for determining inhibition of adhesion, a protein with high an...

journal_title：FEMS microbiology letters

authors： Heinemann C,van Hylckama Vlieg JE,Janssen DB,Busscher HJ,van der Mei HC,Reid G

更新日期：2000-09-01 00:00:00

abstract：:Prevotella ruminicola B1(4) is a strictly anaerobic, Gram-negative, polysaccharide-degrading rumen bacterium. Xylanase activity in this strain was found to be inducible, the specific activity of cells grown on xylan being increased at least 20-fold by comparison with cells grown on glucose. Ten bacteriophage clones ex...

journal_title：FEMS microbiology letters

authors： Gasparic A,Marinsek-Logar R,Martin J,Wallace RJ,Nekrep FV,Flint HJ

更新日期：1995-01-15 00:00:00

abstract：:Mycobacterium leprae incorporated exogenously supplied pyrimidines as bases or nucleosides, but not as a nucleotide, into its nucleic acids. Notably, thymine was incorporated approximately 5 times more rapidly than thymidine by both suspensions of, or intracellular M. leprae. Thymine incorporation was significantly in...

journal_title：FEMS microbiology letters

authors： Wheeler PR

更新日期：1989-01-15 00:00:00

abstract：:The 42- and 51-kDa protein genes of Bacillus sphaericus 1593 have been subcloned independently downstream from the cytA gene promoter of Bacillus thuringiensis serovar israelensis and introduced into a non-mosquitocidal strain of Bacillus thuringiensis. Consequently, each protein was overproduced and accumulated as in...

journal_title：FEMS microbiology letters

authors： Nicolas L,Nielsen-Leroux C,Charles JF,Delécluse A

更新日期：1993-02-01 00:00:00

abstract：:The Mycobacterium tuberculosis murG gene, Rv2153, was expressed in Escherichia coli murG(Ts) strain OV58 on a plasmid under the control of the arabinose-inducible araBAD promoter. Mycobacterium tuberculosis murG rescued the growth of E. coli murG(Ts) at the nonpermissive temperature: transformants were only obtained i...

journal_title：FEMS microbiology letters

authors： Jha RK,Katagihallimath N,Hota SK,Das KS,de Sousa SM

更新日期：2012-01-01 00:00:00

abstract：:In Bacillus subtilis, utilisation of xylose, arabinose and ribose is controlled by the transcriptional factors XylR, AraR and RbsR, respectively. Here we apply the comparative approach to the analysis of these regulons in the Bacillus/Clostridium group. Evolutionary variability of operon structures is demonstrated and...

journal_title：FEMS microbiology letters

authors： Rodionov DA,Mironov AA,Gelfand MS

更新日期：2001-12-18 00:00:00

abstract：:Endpolygalacturonase I from Stereum purpureum has been identified as a causative substance for the silver-leaf disease in apples. It possesses a unique pro-sequence in the C-terminal region that lacks endpolygalacturonases from any other origin. In this study, we analyzed and compared enzymatic characteristics between...

journal_title：FEMS microbiology letters

authors： Hamada S,Toda K,Ogawa S,Kubota K,Miyairi K

更新日期：2015-09-01 00:00:00

abstract：:This study reports on the antifungal activities of statins combined with an antifungal compound secreted by Penicillium chrysogenum, PAF. Several species belonging in the class Zygomycetes are considered to be agents of human or animal mycoses; other species have significance as postharvest plant pathogens. In the pre...

journal_title：FEMS microbiology letters

authors： Galgóczy L,Papp T,Lukács G,Leiter E,Pócsi I,Vágvölgyi C

更新日期：2007-05-01 00:00:00

abstract：:A bacterial isolate, tentatively identified as Pseudomonas sp. strain TR3, was found to utilize the diaryl ester phenylbenzoate as sole source of carbon and energy. This strain has the ability to productively degrade phenylbenzoate and some substituted derivatives by a catabolic sequence which was characterized bioche...

journal_title：FEMS microbiology letters

authors： Reich T,Schmidt S,Fortnagel P

更新日期：1999-07-15 00:00:00

abstract：:A [Fe]-hydrogenase gene (hydA) was cloned from Clostridium paraputrificum M-21 in Escherichia coli using a conserved DNA sequence of clostridial hydrogenase genes amplified by PCR as the probe. The hydA gene consisted of an open reading frame of 1749 bp encoding 582 amino acids with an estimated molecular mass of 64,5...

journal_title：FEMS microbiology letters

authors： Morimoto K,Kimura T,Sakka K,Ohmiya K

更新日期：2005-05-15 00:00:00

abstract：:Incubation of Mycobacterium tuberculosis and M smegmatis cells with the sugar components of their surface-exposed glycans demonstrated that D-arabinose but not alpha-D-glucose or D-mannose, led to the dispersion of the large clumps formed by the bacilli in stationary liquid cultures. These results confirm the presence...

journal_title：FEMS microbiology letters

authors： Anton V,Rougé P,Daffé M

更新日期：1996-11-01 00:00:00

abstract：:We recently identified the phaG(Pp) gene encoding (R)-3-hydroxydecanoyl-ACP:CoA transacylase in Pseudomonas putida, which directly links the fatty acid de novo biosynthesis and polyhydroxyalkanoate (PHA) biosynthesis. An open reading frame (ORF) of which the deduced amino acid sequence shared about 57% identity with P...

journal_title：FEMS microbiology letters

authors： Hoffmann N,Steinbüchel A,Rehm BH

更新日期：2000-03-15 00:00:00

abstract：:In this report we describe the development of a highly stringent and dually regulated promoter system for Shigella flexneri. Dual regulation was provided by utilizing a promoter susceptible to control by the bacteriophage P1 temperature-sensitive C1 repressor that in turn was under the transcriptional control of LacI....

journal_title：FEMS microbiology letters

authors： Schofield DA,Westwater C,Dolan JW,Norris JS,Schmidt MG

更新日期：2002-10-08 00:00:00

abstract：:Hopanoid fingerprints allowed to differentiate bacteria formerly connected to the genus Pseudomonas. Whereas all strains related to Pseudomonas and Ralstonia were devoid of any detectable hopanoid, these pentacyclic triterpenoids were found in the Burkholderia species and in related soil isolates, which contained as m...

journal_title：FEMS microbiology letters

authors： Cvejic JH,Putra SR,El-Beltagy A,Hattori R,Hattori T,Rohmer M

更新日期：2000-02-15 00:00:00

abstract：:In Saccharomyces cerevisiae, genes involved in thiamin pyrophosphate (TPP) synthesis (THI genes) and the pyruvate decarboxylase structural gene PDC5 are transcriptionally induced in response to thiamin starvation. Three positive regulatory factors (Thi2p, Thi3p, and Pdc2p) are involved in the expression of THI genes, ...

journal_title：FEMS microbiology letters

authors： Nosaka K,Esaki H,Onozuka M,Konno H,Hattori Y,Akaji K

更新日期：2012-05-01 00:00:00

abstract：:Lactococcus lactis MG5267 is a plasmid-free strain in which the lactose operon is integrated in the bacterial chromosome. The chromosomal lacG gene which encodes phospho-beta-galactosidase was inactivated by a double cross-over integration event. Unexpectedly, the resultant mutant was shown to retain a Lac-positive ph...

journal_title：FEMS microbiology letters

authors： Payne J,MacCormick CA,Griffin HG,Gasson MJ

更新日期：1996-02-01 00:00:00

abstract：:Mycobacterium avium ssp. paratuberculosis (M. paratuberculosis), the causative agent of Johne's disease, is an important animal pathogen that has also been implicated in human disease. The major proteins expressed by M. paratuberculosis were analyzed by two-dimensional gel electrophoresis, and a superoxide dismutase (...

journal_title：FEMS microbiology letters

authors： Liu X,Feng Z,Harris NB,Cirillo JD,Bercovier H,Barletta RG

更新日期：2001-08-21 00:00:00

abstract：:Halobacillus trueperi accumulates glycine betaine under condition of high osmolarity. A fragment of the glycine betaine transporter betH gene was obtained from the genome of H. trueperi with degenerate primers. Through Southern blot hybridization and inverse PCR, a 5.1 kb EcoRI fragment containing the complete betH ge...

journal_title：FEMS microbiology letters

authors： Lu W,Zhao B,Feng D,Yang S

更新日期：2004-06-15 00:00:00

abstract：:Insertion sequences (ISs) are mobile genetic elements widely distributed among bacteria. Their impact on the bacterial genome is multifold, including transfer of genetic information, shuttle of adaptive traits and influence on the genomic content. As a result, ISs play an important role in the organization, plasticity...

journal_title：FEMS microbiology letters

authors： Lysnyansky I,Calcutt MJ,Ben-Barak I,Ron Y,Levisohn S,Methé BA,Yogev D

更新日期：2009-05-01 00:00:00

abstract：:The presence of insertion sequence IS1 in 70 multiple-antibiotic resistant clinical strains was determined. This 70-strain collection comprised 46 Escherichia coli, 18 Salmonella and 6 Shigella strains. The presence of IS1 was detected in the chromosome and plasmids of 73% and 63% of the strains, respectively, and 51%...

journal_title：FEMS microbiology letters

authors： Ramirez-Santos J,Alvarez G,Cisneros E,Gomez-Eichelmann MC

更新日期：1992-06-01 00:00:00

abstract：:Non-linear dielectric spectroscopy is a novel technique for determining the activity of (predominantly) membranous enzymes as their ability to generate harmonics when excited with a sinusoidal electrical field. In washed suspensions of yeast cells, the ability to generate harmonics is inhibited by low concentrations o...

journal_title：FEMS microbiology letters

authors： Woodward AM,Kell DB

更新日期：1991-11-01 00:00:00

abstract：:Recently, a novel verocytotoxin named VT2y was described which belongs to the STx family and is produced by Escherichia coli isolated from domestic poultry with swollen head syndrome (SHS). The VT2y toxin induced apoptosis in Vero, HeLa, CHO, CEF (primary chicken embryo fibroblast) and PCK (primary chicken kidney) cel...

journal_title：FEMS microbiology letters

authors： Salvadori MR,Yamada AT,Yano T

更新日期：2001-04-01 00:00:00

abstract：:The NH(2)-terminal amino acid sequence of L-threo-3-hydroxyaspartate dehydratase from Pseudomonas sp. T62 showed significant similarity to that of the SRY1 gene product of Saccharomyces cerevisiae (serine racemase in yeast). SRY1 was cloned and expressed in Escherichia coli, and the gene product was purified and parti...

journal_title：FEMS microbiology letters

authors： Wada M,Nakamori S,Takagi H

更新日期：2003-08-29 00:00:00

abstract：:Divergicin 750, a bacteriocin produced by Carnobacterium divergens 750, preferentially inhibited the growth of strains of Carnobacterium and Enterococcus. Selected strains of Listeria monocytogenes and Clostridium perfringens were also inhibited. The bacteriocin was purified to homogeneity by ammonium sulfate precipit...

journal_title：FEMS microbiology letters

authors： Holck A,Axelsson L,Schillinger U

更新日期：1996-02-15 00:00:00

abstract：:Streptococcus pneumoniae strain JNR.7/87 is a highly virulent, type 4 encapsulated Gram-positive bacterium whose transformability has not been tested previously, and whose genome is currently being sequenced. The strain was transformed at very low efficiency by addition of exogenous competence-stimulating peptide: How...

journal_title：FEMS microbiology letters

authors： Bricker AL,Camilli A

更新日期：1999-03-15 00:00:00