Cross-utilization and expression of outer membrane receptor proteins for siderophore uptake by Diamondback moth Plutella xylostella (Lepidoptera: Plutellidae) gut bacteria.

Abstract:

:Siderophore production by entomo- and phytopathogens, plus the cross-utilization of these siderophores and expression of outer membrane receptor proteins (OMRPs) by Diamondback moth (DBM) gut bacterial strains, were all examined. All the tested strains grew in the presence of 2, 2'-dipyridyl, and the Brachybacterium sp. PSGB10, Pseudomonas sp. PRGB06, and Serratia marcescens FLGB16 strains were found to cross-utilize the siderophores of various entomopathogens, including Bacillus thuringiensis. A sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis also showed the presence of the OMRPs responsible for the siderophore cross-utilization. In contrast, Stenotrophomonas sp. PRGB08 was unable to cross-utilize siderophores and did not express OMRPs. Thus, siderophore cross-utilization and OMRP expression by the DBM gut bacterial strains would seem to support the potential for microbial populations in the insect gut to evolve efficient mechanisms to overcome any iron limitation imposed by the host insect and eventually contribute to the defense mechanism of the host insect. Furthermore, it is important to consider that other biologically active metabolites produced by insect gut microorganisms may also confer a protective effect on a host insect species.

journal_name

FEMS Microbiol Lett

authors

Indiragandhi P,Anandham R,Madhaiyan M,Kim GH,Sa T

doi

10.1111/j.1574-6968.2008.01350.x

subject

Has Abstract

pub_date

2008-12-01 00:00:00

pages

27-33

issue

1

eissn

0378-1097

issn

1574-6968

pii

FML1350

journal_volume

289

pub_type

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