Horizontal transfer of nonconjugative plasmids in a colony biofilm of Escherichia coli.

Abstract:

:We tested the possibility of nonconjugative lateral DNA transfer in a colony biofilm of mixed Escherichia coli strains. By simply coculturing a plasmid-free F(-) strain and another F(-) strain harboring a nonconjugative plasmid in a colony biofilm on antibiotic-free agar media, transformed cells were produced within 24-48 h at the frequency of 10(-10)-10(-9) per recipient cell. PCR analysis of the transformed cells demonstrated the occurrence of lateral plasmid transfer. These cells survived until at least day 7 under antibiotic-free conditions. Liquid cultures of the same strains in Luria-Bertani broth produced no or few transformants, suggesting the importance of colony-biofilm formation for plasmid transfer. This is a novel line of evidence indicating that nonconjugative, nonviral horizontal gene transfer can occur between E. coli cells.

journal_name

FEMS Microbiol Lett

authors

Maeda S,Ito M,Ando T,Ishimoto Y,Fujisawa Y,Takahashi H,Matsuda A,Sawamura A,Kato S

doi

10.1111/j.1574-6968.2005.00072.x

keywords:

subject

Has Abstract

pub_date

2006-02-01 00:00:00

pages

115-20

issue

1

eissn

0378-1097

issn

1574-6968

pii

FML072

journal_volume

255

pub_type

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