Abstract:
:Bacteriophages infecting Salmonella spp. were isolated from sewage using soft agar overlays containing three Salmonella serovars and assessed with regard to their potential to control food-borne salmonellae. Two distinct phages, as defined by plaque morphology, structure and host range, were obtained from a single sample of screened sewage. Phage FGCSSa1 had the broadest host range infecting six of eight Salmonella isolates and neither of two Escherichia coli isolates. Under optimal growth conditions for S. Enteritidis PT160, phage infection resulted in a burst size of 139 PFU but was apparently inactive at a temperature typical of stored foods (5 degrees C), even at multiplicity of infection values in excess of 10 000. While neither isolate had characteristics that would make them candidates for biocontrol of Salmonella spp. in foods, phage FGCSSa1 behaved unusually when grown on two Salmonella serotypes at 37 degrees C in that the addition of phages appeared to retard growth of the host, presumably by the lysis of a fraction of the host cell population.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Carey-Smith GV,Billington C,Cornelius AJ,Hudson JA,Heinemann JAdoi
10.1111/j.1574-6968.2006.00217.xsubject
Has Abstractpub_date
2006-05-01 00:00:00pages
182-6issue
2eissn
0378-1097issn
1574-6968pii
FML217journal_volume
258pub_type
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