Involvement of O8-antigen in altering beta-lactam antibiotic susceptibilities in Escherichia coli.

Abstract:

:In spite of being dispensable, O-antigens are believed to facilitate various cellular processes and alter antibiotic sensitivities. Escherichia coli K-12 (CS109) strains are lacking in O-antigens and are reported to be sensitive to antibiotics. To our surprise, E. coli 2443 (expressing O8-antigen) manifested two- to fourfold higher sensitivities toward penicillin and its derivatives than strain CS109. However, sensitivities toward other structurally unrelated antibiotics remained unchanged. To understand the rationale behind such observations, we replaced the rfb locus of strain 2443 with that of E. coli K-12. The beta-lactam sensitivities of 2443 cells with replaced rfb locus appeared to be identical to those for CS109. Therefore, it is quite reasonable to hypothesize the possible involvement of O8-antigen in beta-lactam sensitization.

journal_name

FEMS Microbiol Lett

authors

Sarkar SK,Ghosh AS

doi

10.1111/j.1574-6968.2008.01104.x

subject

Has Abstract

pub_date

2008-05-01 00:00:00

pages

59-64

issue

1

eissn

0378-1097

issn

1574-6968

pii

FML1104

journal_volume

282

pub_type

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