Abstract:
:The spirochaete, Serpulina pilosicoli, is the agent of intestinal spirochaetosis, a diarrhoeal disease of humans and other species. By mechanisms as yet unknown, large numbers of these spirochaetes intimately attach to the colonic mucosa by one cell end. In some infected individuals, the spirochaetes may invade the lamina propria and adjacent tissues, and they may cause spirochaetaemia. To examine S. pilosicoli for pathogenic determinants homologous with Enterobacteria, DNA was extracted from six strains of S. pilosicoli and hybridised at low stringency with DNA probes derived from the inv, ail and yadA genes of Yersinia enterocolitica, the eae gene from enteropathogenic Escherichia coli and a probe derived from the virulence plasmid of Shigella flexneri. No hybridisation of the enterobacterial probes to S. pilosicoli DNA was detected, indicating that these gene sequences, which are known to be involved in the attachment and invasion processes of the other intestinal pathogens, were not present in the spirochaetes.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Hartland EL,Mikosza AS,Robins-Browne RM,Hampson DJdoi
10.1111/j.1574-6968.1998.tb13127.xsubject
Has Abstractpub_date
1998-08-01 00:00:00pages
59-63issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(98)00257-2journal_volume
165pub_type
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