Abstract:
:The aim of this study was to evaluate the ability of a nested PCR system to detect Salmonella senftenberg in raw oysters. The specific primers of the PCR were derived from the invA gene sequence, essential for Salmonella invasiveness into epithelial cells. First, for the extraction of DNA, four methods (guanidine isothiocyanate, E.Z.N.A. Mollusc Kit, Chelex-100, and lysis with detergents) were compared. A nested PCR method combined with 3.5 h pre-enrichment in buffered peptone water (BPW) and DNA extraction by the resin Chelex-100 is proposed for the detection of S. senftenberg in oyster samples. The detection limit of the method is less than 0.1 CFU/ml (<1 CFU/g of oyster). This procedure is shown to be an excellent tool for the sensitive detection of S. senftenberg from naturally contaminated oysters, with results being obtained within 8 h.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Vázquez-Novelle MD,Pazos AJ,Abad M,Sánchez JL,Pérez-Parallé MLdoi
10.1016/j.femsle.2004.12.016keywords:
subject
Has Abstractpub_date
2005-02-01 00:00:00pages
279-83issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(04)00905-Xjournal_volume
243pub_type
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