Secretory production of Arthrobacter levan fructotransferase from recombinant Escherichia coli.

abstract：:The significance of the chronicled role of the yeast transcription factor Adr1p in regulating ETR1 was examined in wild type and isogenic adr1Delta mutant cells. An ETR1-lacZ reporter construct was used to verify Adr1p-dependent gene expression. On solid glycerol medium containing X-gal, wild-type cells expressing the...

journal_title：FEMS microbiology letters

authors： Gurvitz A

更新日期：2009-08-01 00:00:00

abstract：:Pneumocystis carinii pneumonia was induced in rats by chronic corticosteroid immunosuppression. The parasite was separated from virtually all contaminating host cells using the technique of unit gravity sedimentation. Cellular contamination was routinely below 0.02%. The same technique allowed partial separation of th...

journal_title：FEMS microbiology letters

authors： Taylor MB,Easmon CS

更新日期：1990-06-15 00:00:00

abstract：:The discovery of nitrite-dependent anaerobic methane oxidation (n-damo) mediated by 'Candidatus Methylomirabilis oxyfera' with nitrite and methane as substrates has connected biogeochemical carbon and nitrogen cycles in a new way. The paddy fields often carry substantial methane and nitrate, thus may be a favorable ha...

journal_title：FEMS microbiology letters

authors： Zhou L,Wang Y,Long XE,Guo J,Zhu G

更新日期：2014-11-01 00:00:00

abstract：:CRISPR-Cas adaptive immune systems protect bacteria from phage predation, and other foreign genetic elements such as plasmids. Significant advances have been made regarding how CRISPR-Cas systems elicit immunity; however, comparatively little is known about their regulation. To study CRISPR-Cas regulation, we describe...

journal_title：FEMS microbiology letters

authors： Hampton HG,Patterson AG,Chang JT,Taylor C,Fineran PC

更新日期：2019-06-01 00:00:00

abstract：:An enrichment culture which completely degraded fenoxaprop-ethyl (FE) was acquired by using FE as sole carbon source. An efficient FE-degrading strain T1 was isolated from the enrichment culture and identified as Rhodococcus sp. Strain T1 could degrade 94% of 100 mg L(-1) FE within 24 h and the metabolite fenoxaprop a...

journal_title：FEMS microbiology letters

authors： Hou Y,Tao J,Shen W,Liu J,Li J,Li Y,Cao H,Cui Z

更新日期：2011-10-01 00:00:00

abstract：:Cowpea (Vigna unguiculata) and mung bean (Vigna radiata) are important legume crops yet their rhizobia have not been well characterized. In the present study, 62 rhizobial strains isolated from the root nodules of these plants grown in the subtropical region of China were analyzed via a polyphasic approach. The result...

journal_title：FEMS microbiology letters

authors： Zhang YF,Wang ET,Tian CF,Wang FQ,Han LL,Chen WF,Chen WX

更新日期：2008-08-01 00:00:00

abstract：:Trichoderma reesei is a filamentous fungus widely used as an efficient protein producer and known to secrete large quantities of biomass degrading enzymes. Much work has been done aimed at improving the secretion efficiency of this fungus. It is generally accepted that the major bottlenecks in secretion are protein fo...

journal_title：FEMS microbiology letters

authors： Diener SE,Dunn-Coleman N,Foreman P,Houfek TD,Teunissen PJ,van Solingen P,Dankmeyer L,Mitchell TK,Ward M,Dean RA

更新日期：2004-01-30 00:00:00

abstract：:Ruminococcus albus is a Gram-positive bacterium that degrades plant cell walls in the rumen of herbivores. It was described to synthesize two major glycoside-hydrolases (Cel9B and Cel48A), which are exported and anchored at the cell surface. In bacteria, proteins destined to cross the cytoplasmic membrane are synthesi...

journal_title：FEMS microbiology letters

authors： Esbelin J,Martin C,Forano E,Mosoni P

更新日期：2009-05-01 00:00:00

abstract：:Two conservative gene clusters, the S10 ribosomal protein region and one (of the two) set of rRNA genes, were split in a genome crossover rearrangement event in Mycoplasma gallisepticum. As a result of the rearrangement the major part of the S10 ribosomal protein cluster is located upstream of genes for 23S-5S rRNA (r...

journal_title：FEMS microbiology letters

authors： Skamrov A,Feoktistova E,Goldman M,Beabealashvilli R

更新日期：2002-03-05 00:00:00

abstract：:Three heterocytous cyanobacterial strains were isolated from different habitats of Central India, and initial morphological studies indicated them to be members of the genus Nostoc or other closely related genera. Subsequent studies using morphological, ecological, molecular and phylogenetic methods indicated the thre...

journal_title：FEMS microbiology letters

authors： Saraf A,Dawda HG,Suradkar A,Behere I,Kotulkar M,Shaikh ZM,Kumat A,Batule P,Mishra D,Singh P

更新日期：2018-12-01 00:00:00

abstract：:Brucella melitensis possesses an operon with two components: the response regulator OtpR and a putative cAMP-dependent protein kinase regulatory subunit encoded by the BMEI0067 gene. In the previous study, the function of OtpR has been studied, while little is known about the function of the BMEI0067 gene. Using a bio...

journal_title：FEMS microbiology letters

authors： Liu WJ,Dong H,Peng XW,Wu QM

更新日期：2014-07-01 00:00:00

abstract：:The increase in Antimicrobial resistance (AMR) microorganisms has been exacerbated by exposure to antimicrobial drugs (e.g. antibiotics). A solution to AMR may require academic researchers to not only contribute to the drug discovery pipeline through laboratory research, but also to engage and inform non-specialist au...

journal_title：FEMS microbiology letters

authors： Redfern J,Bowater L,Crossley M,Verran J

更新日期：2018-08-01 00:00:00

abstract：:Antimicrobial resistance (AMR) has emerged as one of the most pressing threats to public health. AMR evolution occurs in the clinic but also in the environment, where antibiotics and heavy metals can select and co-select for AMR. While the selective potential of both antibiotics and metals is increasingly well-charact...

journal_title：FEMS microbiology letters

authors： Vos M,Sibleyras L,Lo LK,Hesse E,Gaze W,Klümper U

更新日期：2020-02-01 00:00:00

abstract：:Three tri-substituted spermidines, di-p-coumaroyl-caffeoylspermidine, tri-caffeoylspermidine and tri-p-coumaroylspermidine, isolated from pollen of Quercus alba, were examined for antifungal activity. Both di-p-coumaroyl-caffeoylspermidine and tri-p-coumaroylspermidine reduced mycelial growth of the oat leaf stripe pa...

journal_title：FEMS microbiology letters

authors： Walters D,Meurer-Grimes B,Rovira I

更新日期：2001-07-24 00:00:00

abstract：:Nine clinical isolates of Enterobacteriaceae (six Escherichia coli and three Proteus mirabilis) isolated in three Parisian hospitals between 1989 and 2000 showed a particular extended-spectrum cephalosporin-resistance profile characterized by resistance to cefotaxime and aztreonam but not to ceftazidime. CTX-M-1, CTX-...

journal_title：FEMS microbiology letters

authors： Saladin M,Cao VT,Lambert T,Donay JL,Herrmann JL,Ould-Hocine Z,Verdet C,Delisle F,Philippon A,Arlet G

更新日期：2002-04-09 00:00:00

abstract：:A typing method for Clostridium difficile based on restriction fragment length polymorphisms (RFLP) is described. The technique utilizes commercially available Escherichia coli ribosomal ribonucleic acid (rRNA) as probe material. Probe labelling, hybridization and detection was performed using the Enhanced Chemilumine...

journal_title：FEMS microbiology letters

authors： Bowman RA,O'Neill GL,Riley TV

更新日期：1991-04-15 00:00:00

abstract：:Bordetella pertussis strains contain at least three distinct genes coding for fimbrial subunits, designated fim2, fim3, and fimX. The sequences of the fim2 and fimX genes have been published. Here we present the sequence of the fim3 gene. Proximal and distal to the fim3 gene, regions were observed that could function ...

journal_title：FEMS microbiology letters

authors： Mooi FR,ter Avest A,van der Heide HG

更新日期：1990-01-01 00:00:00

abstract：:We report a rapid and reliable two-step multiplex polymerase chain reaction (PCR) assay to identify the 10 Bacteroides fragilis group species - Bacteroides caccae, B. distasonis, B. eggerthii, B. fragilis, B. merdae, B. ovatus, B. stercoris, B. thetaiotaomicron, B. uniformis and B. vulgatus. These 10 species were firs...

journal_title：FEMS microbiology letters

authors： Liu C,Song Y,McTeague M,Vu AW,Wexler H,Finegold SM

更新日期：2003-05-16 00:00:00

abstract：:Changes in gene expression play a critical role in enhancing the ability of cyanobacteria to survive under cold conditions. In the present study, Spirulina platensis cultures were grown at the optimal growth temperature, in the light, before being transferred to dark conditions at 22 degrees C. Two dimensional-differe...

journal_title：FEMS microbiology letters

authors： Jeamton W,Mungpakdee S,Sirijuntarut M,Prommeenate P,Cheevadhanarak S,Tanticharoen M,Hongsthong A

更新日期：2008-04-01 00:00:00

abstract：:Multidrug resistance plasmids carrying the bla(CMY-2) gene have been identified in Salmonella enterica serovars Typhimurium and Newport from the United States. This gene confers decreased susceptibility to ceftriaxone, and is most often found in strains with concomitant resistance to ampicillin, chloramphenicol, strep...

journal_title：FEMS microbiology letters

authors： Doublet B,Carattoli A,Whichard JM,White DG,Baucheron S,Chaslus-Dancla E,Cloeckaert A

更新日期：2004-04-15 00:00:00

abstract：:Isolated membranes of the extreme haloalkaliphilic archaeon Natronococcus occultus were able to hydrolyze ATP via an ATPase, which required the presence of Mg(2+), high concentrations of NaCl, and a pH value of 9. The native molecular mass of the purified ATPase was 130 kDa and was composed of 74- and 61-kDa subunits....

journal_title：FEMS microbiology letters

authors： Eddy ML,Jablonski PE

更新日期：2000-08-15 00:00:00

abstract：:Cell-free protein synthesis has emerged in recent years as a powerful tool that can potentially transform the production of recombinant proteins. Cell-free protein synthesis harnesses the synthetic power of living cells while eliminating many of the constraints of traditional cell-based gene expression methods. Due to...

journal_title：FEMS microbiology letters

authors： Lee KH,Kim DM

更新日期：2018-09-01 00:00:00

abstract：:Candida albicans yeasts adhered avidly to extracellular matrix (ECM) proteins, type IV collagen, laminin, and fibronectin immobilized on plastic. Type IV collagen showed an increase of adherence of 400% above control values; laminin, 300%; and fibronectin, 150%. In addition, all three (in quantities of 0.02-200 microg...

journal_title：FEMS microbiology letters

authors： Klotz SA

更新日期：1990-03-15 00:00:00

abstract：:Integrons are bacterial genetic elements capable of capturing and expressing potentially adaptive genetic material. Class 1 integrons constitute the most intensely studied group of these elements to date, mainly due to their well-established role in the acquisition and dissemination of antibiotic resistance genes in c...

journal_title：FEMS microbiology letters

authors： Hardwick SA,Stokes HW,Findlay S,Taylor M,Gillings MR

更新日期：2008-01-01 00:00:00

abstract：:Hydrogenotrophic methanogens live in a synthrophic relationship with the human gut microbiota as the terminal part of the anaerobic food chain. Methanobrevibacter smithii of the Methanobacteriales is the prevailing archaeal species. Recently, methylotrophic archaea of the novel order Methanomassiliicoccales were isola...

journal_title：FEMS microbiology letters

authors： Vanderhaeghen S,Lacroix C,Schwab C

更新日期：2015-07-01 00:00:00

abstract：:A gene encoding a phosphomannose isomerase from Streptococcus mutans GS-5 was identified immediately downstream from the fructokinase gene, scrK. Nucleotide sequence analysis of this region revealed an open reading frame (ORF) specifying a putative protein of 316 amino acids. The gene cloned in Escherichia coli expres...

journal_title：FEMS microbiology letters

authors： Sato Y,Yamamoto Y,Kizaki H,Kuramitsu HK

更新日期：1993-11-15 00:00:00

abstract：:Shigella flexneri, which causes shigellosis in humans, evolved from Escherichia coli. The sequencing of Shigella genomes has revealed that a large number of insertion sequence (IS) elements (over 200 elements) reside in the genome. Although the presence of these elements has been noted previously and summarized, more ...

journal_title：FEMS microbiology letters

authors： Zaghloul L,Tang C,Chin HY,Bek EJ,Lan R,Tanaka MM

更新日期：2007-12-01 00:00:00

abstract：:We have previously shown that the secreted M. tuberculosis complex proteins CFP-10 and ESAT-6 form a tight, 1:1 complex, which may represent their functional form. In the work reported here a combination of yeast two-hybrid and biochemical analysis has been used to characterise complex formation between two other pair...

journal_title：FEMS microbiology letters

authors： Lightbody KL,Renshaw PS,Collins ML,Wright RL,Hunt DM,Gordon SV,Hewinson RG,Buxton RS,Williamson RA,Carr MD

更新日期：2004-09-01 00:00:00

abstract：:We tested the possibility of nonconjugative lateral DNA transfer in a colony biofilm of mixed Escherichia coli strains. By simply coculturing a plasmid-free F(-) strain and another F(-) strain harboring a nonconjugative plasmid in a colony biofilm on antibiotic-free agar media, transformed cells were produced within 2...

journal_title：FEMS microbiology letters

authors： Maeda S,Ito M,Ando T,Ishimoto Y,Fujisawa Y,Takahashi H,Matsuda A,Sawamura A,Kato S

更新日期：2006-02-01 00:00:00

abstract：:Emergence of races in Fusarium oxysporum f. sp. lycopersici (Fol) is caused by loss or mutation of at least one avirulence (AVR) gene. The product of AVR1 is a small protein (Avr1) secreted by Fol in tomato xylem sap during infection. This protein triggers Fol race 1 specific resistance (I) in tomato, indicating that ...

journal_title：FEMS microbiology letters

authors： Kashiwa T,Suzuki T,Sato A,Akai K,Teraoka T,Komatsu K,Arie T

更新日期：2016-07-01 00:00:00