Abstract:
:An enrichment culture which completely degraded fenoxaprop-ethyl (FE) was acquired by using FE as sole carbon source. An efficient FE-degrading strain T1 was isolated from the enrichment culture and identified as Rhodococcus sp. Strain T1 could degrade 94% of 100 mg L(-1) FE within 24 h and the metabolite fenoxaprop acid (FA) was identified by HPLC/MS analysis. This strain converted FE by cleavage of the ester bond, but could not further degrade FA. Strain T1 could also efficiently degrade haloxyfop-R-methyl, quizalofop-p-ethyl, cyhalofop-butyl and clodinafop-propargyl. FE hydrolase capable of hydrolysing FE to FA was found in the cell-free extract of strain T1 by zymogram analysis. A novel gene feh encoding FE hydrolase was cloned by shotgun library construction and successfully expressed in Escherichia coli.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Hou Y,Tao J,Shen W,Liu J,Li J,Li Y,Cao H,Cui Zdoi
10.1111/j.1574-6968.2011.02376.xsubject
Has Abstractpub_date
2011-10-01 00:00:00pages
196-203issue
2eissn
0378-1097issn
1574-6968journal_volume
323pub_type
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