Transposon mutagenesis in Staphylococcus epidermidis using the Enterococcus faecalis transposon Tn917.

abstract：:Marine ammonia oxidising bacteria were enriched by incubation of sea water, amended with ammonium sulphate, and subsequent subculture in liquid inorganic medium. PCR primers were designed to be specific for rDNA sequences from ammonia oxidisers belonging to the beta-sub-group of the proteobacteria. These primers were ...

journal_title：FEMS microbiology letters

authors： McCaig AE,Embley TM,Prosser JI

更新日期：1994-07-15 00:00:00

abstract：:Group B streptococci (GBS) represent a very important group of human pathogens. So far little is known about the mechanisms by which these bacteria can cause disease and the bacterial factors involved. One putative virulence factor is the beta antigen of the C protein complex (Bac), which can bind to the Fc region of ...

journal_title：FEMS microbiology letters

authors： Kreikemeyer B,Jerlström PG

更新日期：1999-11-15 00:00:00

abstract：:Aceticlastic methanogens metabolize acetate to methane and carbon dioxide. The central metabolism and the electron transport chains of these organisms have already been investigated. However, no particular attention has been paid to the mechanism by which acetate enters the archaeal cell. In our study we investigated ...

journal_title：FEMS microbiology letters

authors： Welte C,Kröninger L,Deppenmeier U

更新日期：2014-10-01 00:00:00

abstract：:Chemical and molecular taxonomic studies were performed on a representative strain of some lactic acid bacteria of unknown taxonomic position isolated from salmonid fish. The results demonstrate that the fish bacterium represents a new species of the genus Lactococcus for which the name Lactococcus piscium sp. nov. is...

journal_title：FEMS microbiology letters

authors： Williams AM,Fryer JL,Collins MD

更新日期：1990-03-01 00:00:00

abstract：:Fungi of the genus Fusarium are important plant pathogens and contaminants of cereal grains producing different types of mycotoxins. Enniatins are a group of mycotoxins with ionophoric properties frequently detected in North European grains. Within the Fusarium complex responsible for grain infection, Fusarium avenace...

journal_title：FEMS microbiology letters

authors： Kulik T,Jestoi M,Okorski A

更新日期：2011-01-01 00:00:00

abstract：:We investigated whether a subinhibitory concentration of clindamycin (Cli), corresponding to 1/2 the strain-specific minimum inhibitory concentration (MIC), could affect expression and stability of transcripts from genes coding for specific adhesins such as fibronectin binding proteins A (fnbA) and B (fnbB) as well as...

journal_title：FEMS microbiology letters

authors： Blickwede M,Wolz C,Valentin-Weigand P,Schwarz S

更新日期：2005-11-01 00:00:00

abstract：:Using oligonucleotides derived from the N-terminal sequence of a triazine hydrolase from Nocardioides sp. strain C190, two DNA fragments containing trzN were cloned into Escherichia coli and their nucleotide sequences were determined. The 456-amino acid polypeptide predicted from the 1356-bp trzN ORF displayed signifi...

journal_title：FEMS microbiology letters

authors： Mulbry WW,Zhu H,Nour SM,Topp E

更新日期：2002-01-02 00:00:00

abstract：:We found two Shiga toxin producing Escherichia coli O157:H7 strains isolated from humans carrying the stx(1) gene with an IS1203-like element (designated as IS1203v(1)). The IS1203v(1) was inserted into the coding region of the A subunit 7 bp upstream from the TGA termination codon, resulting in a loss of two amino ac...

journal_title：FEMS microbiology letters

authors： Suzuki M,Kondo F,Ito Y,Matsumoto M,Hata M,Oka H,Takahashi M,Sakae K

更新日期：2004-05-01 00:00:00

abstract：:Adenylate kinase isoenzymes localised in the mitochondria and in the cytosol have been detected in extracts of glucose-grown Aspergillus nidulans using specific staining after electrophoresis on cellulose acetate. The isoenzymes have similar Km values for AMP, ADP and MgATP2- but may differ in the mechanism used for i...

journal_title：FEMS microbiology letters

authors： Hoang VQ,Jaklitsch W,Scrutton MC

更新日期：1991-03-01 00:00:00

abstract：:To elucidate the molecular mechanism by which the MexR repressor regulates expression of the MexAB-OprM efflux pump, we investigated MexR and the mexR-mexA intergenic DNA (mexOP) interaction, and transcription of the mexA-lacZ reporter gene containing different lengths of mexOP. Homogeneously purified MexR bound speci...

journal_title：FEMS microbiology letters

authors： Saito K,Eda S,Maseda H,Nakae T

更新日期：2001-02-05 00:00:00

abstract：:The quorum quenching (QQ) activity of endophytic bacteria associated with medicinal plants was explored. Extracts of the Gram-negative Enterobacter sp. CS66 possessed potent N-acylhomoserine lactone (AHL) hydrolytic activity in vitro. Using degenerate primers, we PCR-amplified an open reading frame (denoted aiiE) from...

journal_title：FEMS microbiology letters

authors： Shastry RP,Dolan SK,Abdelhamid Y,Vittal RR,Welch M

更新日期：2018-05-01 00:00:00

abstract：:Pseudomonas aeruginosa are known to have a wide physiological potential allowing them to constantly populate diverse environments leading to severe infections of humans such as septicemia, leg ulcers, and burn wounds. We set out to probe physiological characteristics of P. aeruginosa isolates from diabetic leg ulcers ...

journal_title：FEMS microbiology letters

authors： Buivydas A,Pasanen T,Senčilo A,Daugelavičius R,Vaara M,Bamford DH

更新日期：2013-06-01 00:00:00

abstract：:Eikenella corrodens 33EK(L), a clinical isolate, was assayed for its ability to utilise amino acids as substrates in the reduction of nitrate to nitrite. The metabolism of proline, glutamate, serine and glutamine was found to result in relatively high rates of nitrate reduction. The ability of cells to metabolise thes...

journal_title：FEMS microbiology letters

authors： Gully NJ,Rogers AH

更新日期：1996-06-01 00:00:00

abstract：:The relationship between sugar availability and RTX (repeats in toxin) cytotoxin (leukotoxin) production in the periodontopathic bacterium, Actinobacillus actinomycetemcomitans, was investigated using a chemostat. A actinomycetemcomitans 301-b produced significant amounts of leukotoxin in anaerobic fructose-limited ch...

journal_title：FEMS microbiology letters

authors： Mizoguchi K,Ohta H,Miyagi A,Kurihara H,Takashiba S,Kato K,Murayama Y,Fukui K

更新日期：1997-01-01 00:00:00

abstract：:Quantitative cloning efficiencies for B. megaterium, B. subtilis, and E. coli were compared. Transformation of B. megaterium is less efficient than transformation of B. subtilis or E. coli. The frequency of recombinant clones was equal in E. coli and B. megaterium; both somewhat higher than in B. subtilis. Equivalent ...

journal_title：FEMS microbiology letters

authors： Von Tersch MA,Robbins HL

更新日期：1990-08-01 00:00:00

abstract：:Small colony variants (SCVs) of Pseudomonas aeruginosa NCTC 6750 (WT) were repeatedly isolated in an in vitro kinetic model after exposure to gentamicin (GM). There were minor differences biochemically and in phage and serotyping between the wild type (WT) strain and SCVs. Changes in outer membrane protein profiles we...

journal_title：FEMS microbiology letters

authors： Langford PR,Anwar H,Gonda I,Brown MR

更新日期：1989-10-01 00:00:00

abstract：:During bacterial infection, professional phagocytes are attracted to the site of infection, where they constitute a first line of host cell defense. Their function is to engulf and destroy the pathogens. Thus, bacteria must withstand the bactericidal activity of professional phagocytes, including macrophages to counte...

journal_title：FEMS microbiology letters

authors： Tran SL,Ramarao N

更新日期：2013-10-01 00:00:00

abstract：:The discovery that all hyperthermophiles that have been evaluated have the capacity to reduce Fe(III) has raised the question of whether mechanisms for dissimilatory Fe(III) reduction have been conserved throughout microbial evolution. Many studies have suggested that c-type cytochromes are integral components in elec...

journal_title：FEMS microbiology letters

authors： Childers SE,Lovley DR

更新日期：2001-02-20 00:00:00

abstract：:Three clinical isolates of Salmonella enterica recovered between 2000 and 2003 in France and Senegal were found to produce extended-spectrum beta-lactamase CTX-M-15. The two isolates from Senegal were recovered from stool of a hospitalized patient with gastroenteritis in 2000 and from an urine specimen of an out-patie...

journal_title：FEMS microbiology letters

authors： Weill FX,Perrier-Gros-Claude JD,Demartin M,Coignard S,Grimont PA

更新日期：2004-09-15 00:00:00

abstract：:Fusobacterium nucleatum is a common oral anaerobe associated with gingivitis, periodontal disease and preterm deliveries. Coaggregation among oral bacteria is considered to be a significant factor in dental plaque development. Adhesion to host cells was suggested to be important for the F. nucleatum virulence associat...

journal_title：FEMS microbiology letters

authors： Bachrach G,Ianculovici C,Naor R,Weiss EI

更新日期：2005-07-15 00:00:00

abstract：:The first method for enucleation of yeast Saccharomyces cerevisiae is reported. Various strains, including some killer strain and respiratory-deficient mutants of Saccharomyces cerevisiae were enucleated after treatment with cytochalasin B. Removal of nuclei from protruding sphaeroplasts was induced by centrifugation ...

journal_title：FEMS microbiology letters

authors： Salek AT

更新日期：1993-10-01 00:00:00

abstract：:We have investigated the iron-dependent regulation of the psbA gene, encoding the enzyme L-ornithine N(5)-oxygenase in the rhizobacterium Pseudomonas B10. We have cloned and characterized a Pseudomonas B10 gene, designated psbS, required for psbA expression. PsbS is endowed with structural and functional features of e...

journal_title：FEMS microbiology letters

authors： Leoni L,Ambrosi C,Petrucca A,Visca P

更新日期：2002-03-05 00:00:00

abstract：:Three tri-substituted spermidines, di-p-coumaroyl-caffeoylspermidine, tri-caffeoylspermidine and tri-p-coumaroylspermidine, isolated from pollen of Quercus alba, were examined for antifungal activity. Both di-p-coumaroyl-caffeoylspermidine and tri-p-coumaroylspermidine reduced mycelial growth of the oat leaf stripe pa...

journal_title：FEMS microbiology letters

authors： Walters D,Meurer-Grimes B,Rovira I

更新日期：2001-07-24 00:00:00

abstract：:The hybrid Pseudomonas cepacia strain JHR22 was tested for its ability to degrade Aroclor 1221 in soil. The influence of supplements--mineral salts and trace elements--on the degradation was investigated. Disappearance of Aroclor 1221 congeners, occurrence of metabolites, and release of chloride were measured under di...

journal_title：FEMS microbiology letters

authors： Havel J,Reineke W

更新日期：1993-04-01 00:00:00

abstract：:The commercialization of the n-butanol bioprocess is largely dependent on the price of feedstocks. Renewable cellulose appears to be an appealing feedstock. The microbial production of n-butanol still remains challenging because of the limited availability of intracellular NADH. To address this issue, an Escherichia c...

journal_title：FEMS microbiology letters

authors： Saini M,Chiang CJ,Li SY,Chao YP

更新日期：2016-02-01 00:00:00

abstract：:Ferric uptake regulator (Fur) proteins are widely recognized as repressors that in many prokaryotes regulate a large number of genes involved in iron homeostasis and oxidative stress response. In our study, we were able to identify the complete sequence of the fur gene from Microcystis aeruginosa using inverse-polymer...

journal_title：FEMS microbiology letters

authors： Martin-Luna B,Hernandez JA,Bes MT,Fillat MF,Peleato ML

更新日期：2006-01-01 00:00:00

abstract：:In the yearly Internationally Genetically Engineered Machines (iGEM) competition, teams of Bachelor's and Master's students design and build an engineered biological system using DNA technologies. Advising an iGEM team poses unique challenges due to the inherent difficulties of mounting and completing a new biological...

journal_title：FEMS microbiology letters

authors： Tsui J,Meyer AS

更新日期：2016-07-01 00:00:00

abstract：:Staphylococcus aureus lipase (SAL) is known to possess broad substrate specificity for triacylglycerides. We found that a sub-minimum inhibitory concentration of farnesol (1000 mg L(-1)) inhibits this lipase activity on a Mueller-Hinton agar containing 1% Tween substrates. A quantitative lipase assay using p-nitrophen...

journal_title：FEMS microbiology letters

authors： Kuroda M,Nagasaki S,Ito R,Ohta T

更新日期：2007-08-01 00:00:00

abstract：:This study investigated 247 Escherichia coli isolates collected from four cattle farms to characterize aminoglycoside-modifying enzyme (AME) genes, their plasmid replicons and transferability. Out of 247 isolates a high number of isolates (total 202; 81.78%) were found to be resistant to various antibiotics by disc di...

journal_title：FEMS microbiology letters

authors： Belaynehe KM,Shin SW,Hong-Tae P,Yoo HS

更新日期：2017-08-01 00:00:00

abstract：:Ceramide mono (CMH) or dihexoside (CDH) fractions from Trypanosoma cruzi (Dm28c clone) were identified as glucosyl and lactosylceramides containing non-hydroxylated fatty acids. The di-glycosylated form was much more efficiently recognized by sera from T. cruzi-immunized rabbits, indicating that glycosylation influenc...

journal_title：FEMS microbiology letters

authors： Villas-Boas MH,Wait R,Silva RB,Rodrigues ML,Barreto-Bergter E

更新日期：2005-03-01 00:00:00