Abstract:
:Staphylococcus aureus lipase (SAL) is known to possess broad substrate specificity for triacylglycerides. We found that a sub-minimum inhibitory concentration of farnesol (1000 mg L(-1)) inhibits this lipase activity on a Mueller-Hinton agar containing 1% Tween substrates. A quantitative lipase assay using p-nitrophenyl palmitate (pNPP) revealed that the inhibitory action of farnesol appears to be the result of the inhibition of lipase activity rather than of its secretion into the culture medium. The inhibition was observed in all the tested 8 methicillin-susceptible S. aureus and 31 methicillin-resistant S. aureus clinical isolates. Using homogeneous lipase purified by hydrophobic interaction chromatography, it was revealed that farnesol could competitively inhibit the lipase activity against the substrate pNPP.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Kuroda M,Nagasaki S,Ito R,Ohta Tdoi
10.1111/j.1574-6968.2007.00772.xsubject
Has Abstractpub_date
2007-08-01 00:00:00pages
28-34issue
1eissn
0378-1097issn
1574-6968pii
FML772journal_volume
273pub_type
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