Abstract:
:A gene library of Neisseria meningitidis B15 P1.16 DNA was established in lambda Zap II and clones containing DNA encoding transferrin binding protein 1 (TBP-1) identified following hybridisation with a 63-bp DNA probe based on the codon assignment for the first 21 N-terminal amino acids of TBP-1. Sequencing of the cloned DNA demonstrated that all of the intergenic DNA (i.e. upstream of tbp-1 running through to the 3' end of the transferrin-binding protein 2 gene) and approx. 15% of tbp-1 had been cloned. The complete gene was generated using a polymerase chain reaction, with the primer for the 3' end being based on tbp-A of N. gonorrhoeae, and the approx. 2.9-kb DNA product cloned into pGem-3Z. The expressed protein (approx. 100 kDa) reacted with antiserum to an N-terminal peptide of TBP-1. In addition, the native product was surface-expressed by Escherichia coli and bound human transferrin.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Palmer HM,Powell NB,Ala'Aldeen DA,Wilton J,Borriello SPdoi
10.1111/j.1574-6968.1993.tb06310.xsubject
Has Abstractpub_date
1993-06-15 00:00:00pages
139-45issue
2eissn
0378-1097issn
1574-6968journal_volume
110pub_type
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