Identification of in vivo essential genes from Pseudomonas aeruginosa by PCR-based signature-tagged mutagenesis.

Abstract:

:We adapted PCR-based signature-tagged mutagenesis (STM) to Pseudomonas aeruginosa. A collection of 1056 mutants was screened in a chronic lung infection rat model. Thirteen mutants were confirmed to be attenuated. Analysis revealed that these STM mutants represented transposon insertions into eight genes previously described in databases, three genes encoding proteins sharing identity with hypothetical proteins and two genes that shared no significant identity with sequences in databases. Five strains mutated in genes involved in protein degradation, stress tolerance, cation transport, ABC transporter, and an unknown protein were shown to be highly attenuated when tested individually in the rat chronic lung infection model.

journal_name

FEMS Microbiol Lett

authors

Lehoux DE,Sanschagrin F,Levesque RC

doi

10.1111/j.1574-6968.2002.tb11162.x

keywords:

subject

Has Abstract

pub_date

2002-04-23 00:00:00

pages

73-80

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378109702005979

journal_volume

210

pub_type

杂志文章
  • The Tat protein transport system: intriguing questions and conundrums.

    abstract::The Tat machinery catalyzes the transport of folded proteins across the cytoplasmic membrane in bacteria and the thylakoid membrane in plants. Transport occurs only in the presence of an electric field (Δψ) and/or a pH (ΔpH) gradient, and thus, Tat transport is considered to be dependent on the proton motive force (pm...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fny123

    authors: Hamsanathan S,Musser SM

    更新日期:2018-06-01 00:00:00

  • Genomic 3' terminal sequence comparison of three isolates of rabbit haemorrhagic disease virus.

    abstract::Comparison of sequence data is necessary in older to investigate virus origins, identify features common to virulent strains, and characterize genomic organization within virus families. A virulent caliciviral disease of rabbits recently emerged in China. We have sequenced 1100 bases from the 3' ends of two independen...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90486-8

    authors: Milton ID,Vlasak R,Nowotny N,Rodak L,Carter MJ

    更新日期:1992-05-15 00:00:00

  • Efflux of choline and glycine betaine from osmoregulating cells of Escherichia coli.

    abstract::We present evidence that glycine betaine (betaine) which was synthesized from choline was excreted and reaccumulated in osmoregulating cells of Escherichia coli. Choline which was accumulated in bet mutants defective in betaine synthesis was shown to be excreted in response to betaine uptake. Our data suggest that E. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90395-5

    authors: Lamark T,Styrvold OB,Strøm AR

    更新日期:1992-09-15 00:00:00

  • Identification of mature appressorium-enriched transcripts in Magnaporthe grisea, the rice blast fungus, using suppression subtractive hybridization.

    abstract::We have constructed a fungal subtractive suppressive library enriched in genes expressed during appressorium maturation in Magnaporthe grisea. Sequencing of 250 clones from the subtracted appressorium cDNA library revealed 142 unique genes, represented by 155 non-redundant ESTs (expressed sequence tags). Of these ESTs...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.02.032

    authors: Lu JP,Liu TB,Lin FC

    更新日期:2005-04-01 00:00:00

  • Novel plasmids in clinical strains of Streptococcus pneumoniae.

    abstract::Small plasmids were found in two clinical isolates of Streptococcus pneumoniae from Spain (strains 671 and 678) and in one strain (SpR) isolated in Germany. All three strains contained one plasmid (2.75 to 3.1 kb) which is related to the only previously described pneumococcal plasmid, pDP1. Strains 678 and SpR carried...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(91)90019-7

    authors: Sibold C,Markiewicz Z,Latorre C,Hakenbeck R

    更新日期:1991-01-01 00:00:00

  • Expression of a new porin 'OmpE' by strains of Salmonella enteritidis.

    abstract::Strains of Salmonella enteritidis expressed a novel porin with a subunit size of 35.5 kDa as shown by SDS-PAGE. This protein was expressed as a major outer membrane protein (MOMP) when grown on nutrient agar, McConkey agar or blood agar, or in Tris-succinate medium; but was only produced in trace amounts when strains ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(93)90017-v

    authors: Chart H,Frost JA,Rowe B

    更新日期:1993-05-15 00:00:00

  • The role of quorum sensing in chronic cystic fibrosis Pseudomonas aeruginosa infections.

    abstract::Studies on cultured cells and in infection models have shown that cell density-dependent quorum-sensing (QS) controls many of the known virulence factors of Pseudomonas aeruginosa. However, it is less clear what role QS plays in chronic human lung infections associated with cystic fibrosis (CF). The involvement of QS ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.2008.01394.x

    authors: Winstanley C,Fothergill JL

    更新日期:2009-01-01 00:00:00

  • New genus-specific primers for the PCR identification of novel isolates of the genus Streptomonospora.

    abstract::The halophilic actinomycete genus Streptomonospora, forming a distinct branch in the 16S rRNA gene phylogenetic tree adjacent to the genera Nocardiopsis and Thermobifida, was first proposed by Cui et al. to accommodate the species type Streptomonospora salina. During a biodiversity and taxonomic study on halophilic fi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00402.x

    authors: Zhi XY,Tang SK,Li WJ,Xu LH,Jiang CL

    更新日期:2006-10-01 00:00:00

  • Interactions between statins and Penicillium chrysogenum antifungal protein (PAF) to inhibit the germination of sporangiospores of different sensitive Zygomycetes.

    abstract::This study reports on the antifungal activities of statins combined with an antifungal compound secreted by Penicillium chrysogenum, PAF. Several species belonging in the class Zygomycetes are considered to be agents of human or animal mycoses; other species have significance as postharvest plant pathogens. In the pre...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00661.x

    authors: Galgóczy L,Papp T,Lukács G,Leiter E,Pócsi I,Vágvölgyi C

    更新日期:2007-05-01 00:00:00

  • Oligonucleotide microarray for identification of Bacillus anthracis based on intergenic transcribed spacers in ribosomal DNA.

    abstract::We developed a DNA microarray for identification of Bacillus anthracis and other phylogenetic groupings within the "Bacillus cereus group". Nucleotide sequences of 16S-23S ribosomal DNA internal transcribed spacers containing genes for tRNA(Ile) from 52 B. anthracis strains were found to be identical to sequences from...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.09.042

    authors: Nübel U,Schmidt PM,Reiss E,Bier F,Beyer W,Naumann D

    更新日期:2004-11-15 00:00:00

  • Temperature-sensitive recombinant subtilisin protease variants that efficiently degrade molecular biology enzymes.

    abstract::We used error-prone PCR to generate mutations in a subtilisin protease-encoding gene, and screened for recombinants that expressed temperature-sensitive (TS) variants. From the dozens of mutations that we detected in the recombinant genes we found that those mutations that affected aspartate-75 had the most profound e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnaa162

    authors: Thompson VC,McGuire BE,Frier MS,Legg MSG,Dyer TW,Gudavicius G,Potter S,Nano FE

    更新日期:2020-10-16 00:00:00

  • Yersinia pestis lacZ expresses a beta-galactosidase with low enzymatic activity.

    abstract::Although very little, if any, beta-galactosidase activity is detected in Yersinia pestis by a standard Miller assay, we found that Y. pestis KIM6+ cells formed blue colonies on plates containing 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal). Searches of the Y. pestis genome databases revealed the presence of n...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2005.00039.x

    authors: Bobrov AG,Perry RD

    更新日期:2006-02-01 00:00:00

  • Evaluation of the catalase promoter for expressing the alkaline xylanase gene (alx) in Aspergillus niger.

    abstract::Aspergillus niger represents a promising host for the expression of recombinant proteins, but only a few expression systems are available for this organism. In this study, the inducible catalase promoter (PcatR) from A. niger was characterized. For this, constructs were developed and checked for the expression of the ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02454.x

    authors: Sharma R,Katoch M,Govindappa N,Srivastava PS,Sastry KN,Qazi GN

    更新日期:2012-02-01 00:00:00

  • Enhancement and repression of cyanide-insensitive respiration in Aspergillus niger.

    abstract::The biosynthesis of the alternative oxidase protein consisting of cyanide-insensitive and salicylhydroxamic acid-sensitive respiration in Aspergillus niger was investigated. Cyanide-insensitive respiration was enhanced by addition of antimycin A to the incubation mixture, but repressed by the addition of cycloheximide...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08393.x

    authors: Kirimura K,Matsui T,Sugano S,Usami S

    更新日期:1996-08-01 00:00:00

  • Catalytic and structural properties of IRT-21 beta-lactamase (TEM-77) from a co-amoxiclav-resistant Proteus mirabilis isolate.

    abstract::Proteus mirabilis strain MAG1, a clinical isolate that is resistant to broad-spectrum penicillins and co-amoxiclav, produces inhibitor-resistant TEM (IRT)-21, a novel mutant of TEM beta-lactamase. This enzyme has a pI of 5.2 and is derived from the bla(TEM-1a) gene ancestor. It contains two major amino acid substituti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10945.x

    authors: Mammeri H,Gilly L,Laurans G,Vedel G,Eb F,Paul G

    更新日期:2001-12-18 00:00:00

  • Modified peptidoglycan precursors produced by glycopeptide-resistant enterococci.

    abstract::Cytoplasmic precursors of the peptidoglycan biosynthetic pathway were purified from vancomycin-treated, glycopeptide-sensitive and -resistant strains of Enterococcus faecium. Resistance was due to production of a modified precursor, UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-lactate, where lactate was identified on the basi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90608-q

    authors: Messer J,Reynolds PE

    更新日期:1992-07-01 00:00:00

  • Fatty acids synthesized by oral treponemes in chemically defined media.

    abstract::OMIZ-W68, a chemically defined medium that contains no long-chain fatty acids and yet supports in vitro proliferation of a wide range of fastidious oral anaerobes, is described. The type strains of Treponema denticola, Treponema lecithinolyticum, Treponema maltophilum, Treponema pectinovorum, Treponema socranskii, and...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00609.x

    authors: Wyss C

    更新日期:2007-04-01 00:00:00

  • The use of writing assignments to help students synthesize content in upper-level undergraduate biology courses.

    abstract::Biology education is undergoing a transformation toward a more student-centered, inquiry-driven classroom. Many educators have designed engaging assignments that are designed to help undergraduate students gain exposure to the scientific process and data analysis. One of these types of assignments is use of a grant pr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx024

    authors: Sparks-Thissen RL

    更新日期:2017-02-01 00:00:00

  • Spiroethers of German chamomile inhibit production of aflatoxin G and trichothecene mycotoxin by inhibiting cytochrome P450 monooxygenases involved in their biosynthesis.

    abstract::The essential oil of German chamomile showed specific inhibition toward aflatoxin G(1) (AFG(1)) production, and (E)- and (Z)-spiroethers were isolated as the active compounds from the oil. The (E)- and (Z)-spiroethers inhibited AFG(1) production of Aspergillus parasiticus with inhibitory concentration 50% (IC(50)) val...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01195.x

    authors: Yoshinari T,Yaguchi A,Takahashi-Ando N,Kimura M,Takahashi H,Nakajima T,Sugita-Konishi Y,Nagasawa H,Sakuda S

    更新日期:2008-07-01 00:00:00

  • Multiple restriction-modification systems are present in rumen treponemes.

    abstract::Type II restriction endonucleases were purified by heparin-sepharose followed by ion chromatography from Treponema strains. The results indicate that in addition to frequently cutting GATC-specific restriction enzymes, the tested strains also possess rarely cutting endonucleases. The purified restriction endonucleases...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.07.032

    authors: Piknova M,Javorsky P,Pristas P

    更新日期:2005-10-01 00:00:00

  • PCR primers specific for the genus Tuber reveal the presence of several truffle species in a truffle-ground.

    abstract::Truffles are hypogeous Ascomycete fungi belonging to the genus Tuber and forming fruiting bodies highly prized for their taste and aroma. The identification of the genus Tuber and its species is important to investigate their ecology and avoid fraud in the food market. As genus-specific primers are not available, the ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01655.x

    authors: Zampieri E,Mello A,Bonfante P,Murat C

    更新日期:2009-08-01 00:00:00

  • Accumulation of ppGpp in Streptococcus pyogenes and Streptococcus rattus following amino acid starvation.

    abstract::We have re-examined the stringent response of Streptococcus rattus and Streptococcus pyogenes, two organisms that had originally been reported not to accumulate ppGpp following amino acid deprivation. We conclude that ppGpp does accumulate when S. rattus and S. pyogenes are deprived of isoleucine by mupirocin addition...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb12836.x

    authors: Whitehead KE,Webber GM,England RR

    更新日期:1998-02-01 00:00:00

  • Construction of mini-Tn10luxABcam/Ptac-ATS and its use for developing a bacteriophage that transduces bioluminescence to Escherichia coli O157:H7.

    abstract::Mini-Tn10luxABcam/Ptac-ATS was constructed in order to develop a luciferase-transducing bacteriophage for detecting Escherichia coli O157:H7. The transposon was designed to deliver a 3.6-kb insertion that confers n-decanal-dependent bioluminescence and resistance to chloramphenicol and was constructed using mini-Tn10c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb08909.x

    authors: Waddell TE,Poppe C

    更新日期:2000-01-15 00:00:00

  • Structure of the Escherichia coli 0104 polysaccharide and its identity with the capsular K9 polysaccharide.

    abstract::The 0104 antigen (lipopolysaccharide, LPS) of Escherichia coli has an acidic O specific polysaccharide. From the aqueous phase of a phenol water extraction of E. coli O104: K-, a fraction was obtained by ultracentrifugation and Cetavlon precipitation of the supernatant, which was enriched in long-chain LPS. Compositio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90673-c

    authors: Kogan G,Jann B,Jann K

    更新日期:1992-03-01 00:00:00

  • Identification of a P2-related prophage remnant locus of Photorhabdus luminescens encoding an R-type phage tail-like particle.

    abstract::Analysis of the Photorhabdus luminescens genome sequence revealed that the pts region is related to the tail synthesis gene core of the P2 phage. The pts locus encodes a DNA invertase homologue. PCR-RFLP analysis showed the two potential tail fiber regions of the pts locus present DNA inversions. Electron microscopy r...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.02.009

    authors: Gaudriault S,Thaler JO,Duchaud E,Kunst F,Boemare N,Givaudan A

    更新日期:2004-04-15 00:00:00

  • Cloning and characterization of genes encoding an enzyme which oxidizes dimethyl sulfide in Acinetobacter sp. strain 20B.

    abstract::Acinetobacter sp. strain 20B was isolated based on the ability to utilize dimethyl sulfide as the sole sulfur source. Since strain 20B oxidized indole as well as dimethyl sulfide, indigo production by recombinant Escherichia coli clones carrying Acinetobacter DNA was used as a selection for cloning genes encoding dime...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb12692.x

    authors: Horinouchi M,Kasuga K,Nojiri H,Yamane H,Omori T

    更新日期:1997-10-01 00:00:00

  • Binding study of AfsK, a Ser/Thr kinase from Streptomyces coelicolor A3(2) and S-adenosyl-L-methionine.

    abstract::Streptomyces coelicolor A3(2) produces an antibiotic, actinorhodin, which belongs to the aromatic polyketides and which can function as an acid/base indicator. Its production results in the death of microorganisms in the vicinity of S. coelicolor A3(2), and this phenomenon can be used in concert with biopesticides. Th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00531.x

    authors: Lee Y,Kim K,Suh JW,Rhee S,Lim Y

    更新日期:2007-01-01 00:00:00

  • Current climate for digital game-based learning of science in further and higher education.

    abstract::Digital game-based learning (DGBL) is being used increasingly as an alternative learning tool to teach science in further and higher education. A variety of digital game formats currently exist for science learning, alongside diverse methods for their implementation and evaluation. This paper aims to provide a broad s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fny237

    authors: Brown CL,Comunale MA,Wigdahl B,Urdaneta-Hartmann S

    更新日期:2018-11-01 00:00:00

  • Mycobacterium leprae infection of human Schwann cells depends on selective host kinases and pathogen-modulated endocytic pathways.

    abstract::Mycobacterium leprae, an obligate intracellular pathogen, shows a unique tropism for Schwann cells (SC). This leads to the peripheral neuropathy disorder observed in leprosy. In this study, we investigated signal transduction events and the intracellular fate of M. leprae during the interaction of the microorganism wi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.08.007

    authors: Alves L,de Mendonça Lima L,da Silva Maeda E,Carvalho L,Holy J,Sarno EN,Pessolani MC,Barker LP

    更新日期:2004-09-15 00:00:00

  • Detection and quantification of virulent Aeromonas hydrophila in channel catfish tissues following waterborne challenge.

    abstract::The aim of this study was to understand the pathogenesis of motile aeromonas septicemia caused by an emergent, high virulent Aeromonas hydrophila (vAh) in channel catfish, Ictalurus punctatus Adipose fin clipped catfish were challenged with vAh using a waterborne challenge method, and the distribution of vAh over a ti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw080

    authors: Zhang D,Moreira GS,Shoemaker C,Newton JC,Xu DH

    更新日期:2016-05-01 00:00:00