Isolation of genes encoding beta-D-xylanase, beta-D-xylosidase and alpha-L-arabinofuranosidase activities from the rumen bacterium Prevotella ruminicola B1(4).

abstract：:Four distinct bands of cysteine proteinase activity were detected when stationary-phase populations of Leishmania mexicana mexicana were subjected to gelatin-SDS-PAGE. The highest mobility band contained at least three isoforms separable by mono Q anion exchange chromatography. These high mobility activities were dist...

journal_title：FEMS microbiology letters

authors： Robertson CD,Coombs GH

更新日期：1992-07-01 00:00:00

abstract：:The occurrence of highly variable penicillin-binding proteins (PBPs) in penicillin-resistant Streptococcus pneumoniae suggested that transfer of homologous genes from related species may be involved in resistance development. Antiserum and monoclonal antibodies raised against PBPs 1a and 2b from the susceptible S. pne...

journal_title：FEMS microbiology letters

authors： Chalkley L,Schuster C,Potgieter E,Hakenbeck R

更新日期：1991-12-15 00:00:00

abstract：:Within the phylum Bacteroidetes, the gyrB gene, encoding for the B subunit of the DNA gyrase, has been used as a phylogenetic marker for several genera closely related to Flavobacterium. The phylogenies of the complete 16S rRNA gene and the gyrB gene were compared for 33 Antarctic Flavobacterium isolates and 23 type s...

journal_title：FEMS microbiology letters

authors： Peeters K,Willems A

更新日期：2011-08-01 00:00:00

abstract：:Microbial competition for carbon sources is a primary determinant of rhizosphere ecology. We employed the PCR to examine the population fluctuations of a symbiotic nitrogen-fixing bacterium (Rhizobium etli) during the first 11 days following inoculation of Phaseolus vulgaris seedlings grown in the presence or absence ...

journal_title：FEMS microbiology letters

authors： Petersen DJ,Srinivasan M,Chanway CP

更新日期：1996-09-01 00:00:00

abstract：:This study was initiated to establish whether inhibition of growth of yeasts by medium-chain fatty acids resembled that caused by weak-acid preservatives or uncouplers. Unlike sorbic acid and 2,4-dinitrophenol, decanoic acid caused rapid cell death at its inhibitory concentration. This suggested a mode of action by me...

journal_title：FEMS microbiology letters

authors： Stratford M,Anslow PA

更新日期：1996-08-15 00:00:00

abstract：:Agrobacterium tumefaciens is the causal agent of crown gall disease and is a vector for DNA transfer in transgenic plants. The transformation process by A. tumefaciens has been widely studied, but the attachment stage has not been well characterized. Most measurements of attachment have used microscopy and colony coun...

journal_title：FEMS microbiology letters

authors： Petrovicheva A,Joyner J,Muth TR

更新日期：2017-10-02 00:00:00

abstract：:Staphylococcus aureus represents the most prevalent cause of food-borne intoxications worldwide. While being repressed by competing bacteria in most matrices, this pathogen exhibits crucial competitive advantages during growth at high salt concentrations or low pH, conditions frequently encountered in food production ...

journal_title：FEMS microbiology letters

authors： Sihto HM,Tasara T,Stephan R,Johler S

更新日期：2014-07-01 00:00:00

abstract：:The cea-kil operon of the ColE1 plasmid is negatively regulated by the LexA-repressor and therefore, it is under the control of SOS regulation. We constructed a gene fusion between the cea and lacZ genes. Expression of the translational fusion can be easily detected by monitoring the levels of beta-galactosidase. Sinc...

journal_title：FEMS microbiology letters

authors： Schumann W,Songür N,Miltenburger HG

更新日期：1991-07-01 00:00:00

abstract：:An isolation procedure was developed to provide within one day microcystin-LR, a cyclic heptapeptide toxin from Microcystis aeruginosa PCC 7806. After ODS (octadecylsilyl) solid phase extraction, the crude toxin fraction was chromatographed using a strong anion exchange column. The toxin was eluted with 0.02 M ammoniu...

journal_title：FEMS microbiology letters

authors： Martin C,Sivonen K,Matern U,Dierstein R,Weckesser J

更新日期：1990-03-01 00:00:00

abstract：:The fatty acid composition during the transformation of Trypanosoma cruzi epimastigotes into metacyclic trypomastigotes (metacyclogenesis) was analysed by gas-liquid chromatography and mass spectrometry. Significant qualitative and quantitative changes in the fatty acid composition occurred during incubation of epimas...

journal_title：FEMS microbiology letters

authors： Esteves MG,Gonzales-Perdomo M,Alviano CS,Angluster J,Goldenberg S

更新日期：1989-05-01 00:00:00

abstract：:The American Society for Microbiology's curricular guidelines for Introductory Microbiology highlighted key laboratory skills in the isolation, visualization and identification of microorganisms as core learning objectives in the discipline. Since the publication of these guidelines in 2012, there has been a paucity o...

journal_title：FEMS microbiology letters

authors： Wang JTH,Huston WM,Johanesen P,Lloyd M,Waller KL

更新日期：2018-10-01 00:00:00

abstract：:Analysis of the Photorhabdus luminescens genome sequence revealed that the pts region is related to the tail synthesis gene core of the P2 phage. The pts locus encodes a DNA invertase homologue. PCR-RFLP analysis showed the two potential tail fiber regions of the pts locus present DNA inversions. Electron microscopy r...

journal_title：FEMS microbiology letters

authors： Gaudriault S,Thaler JO,Duchaud E,Kunst F,Boemare N,Givaudan A

更新日期：2004-04-15 00:00:00

abstract：:Bacterial swarming motility is a flagella-dependent translocation on the surface environment. It has received extensive attention as a population behavior involving numerous genes. Here, we report that Citrobacter freundii, an opportunistic pathogen, exhibits swarming movement on a solid medium surface with appropriat...

journal_title：FEMS microbiology letters

authors： Cong Y,Wang J,Chen Z,Xiong K,Xu Q,Hu F

更新日期：2011-04-01 00:00:00

abstract：:The branched-chain amino acid permease Bap2p is a transport system for leucine, isoleucine, and valine in Saccharomyces cerevisiae, and its synthesis is regulated transcriptionally. However, the downregulation mechanisms of Bap2p have not been established. Here we demonstrate that the C-terminal region of Bap2p plays ...

journal_title：FEMS microbiology letters

authors： Omura F,Kodama Y,Ashikari T

更新日期：2001-01-15 00:00:00

abstract：:The bft gene encoding Bacteroides fragilis toxin (BFT) has been devided into two subtypes, bft-1 and bft-2. We found a novel subtype by sequencing a segment of the bft gene from 64 enterotoxigenic B. fragilis (ETBF) strains isolated in Japan. The 1548-bp nucleotide sequences of the new bft, the bft-1, and bft-2 genes ...

journal_title：FEMS microbiology letters

authors： Kato N,Liu CX,Kato H,Watanabe K,Tanaka Y,Yamamoto T,Suzuki K,Ueno K

更新日期：2000-01-01 00:00:00

abstract：:Methanobacterium barkeri was found to catalyze the reductive dehalogenation of trichlorofluoromethane (CFC-11), also known as FREON 11. Products detected were CHFCl2, CH2FCl, CO and fluoride. ...

journal_title：FEMS microbiology letters

authors： Krone UE,Thauer RK

更新日期：1992-01-01 00:00:00

abstract：:Using natural rubber latex as the sole source of carbon and energy 50 rubber-degrading bacteria were isolated. Out of those 50 isolates, 33 were identified as Streptomyces species and 8 as Micromonospora species. Screening of 1220 bacteria obtained from different culture collections revealed 46 additional rubber-degra...

journal_title：FEMS microbiology letters

authors： Jendrossek D,Tomasi G,Kroppenstedt RM

更新日期：1997-05-15 00:00:00

abstract：:Bacillus cereus strains involved in food poisoning cases of the diarrheal type may produce two different enterotoxin complexes. To facilitate the identification of hemolysin BL-enterotoxin complex (HBL) and/or the nonhemolytic enterotoxin (NHE) producing colonies a colony immunoblot procedure was developed, which allo...

journal_title：FEMS microbiology letters

authors： Moravek M,Wegscheider M,Schulz A,Dietrich R,Bürk C,Märtlbauer E

更新日期：2004-09-01 00:00:00

abstract：:The TBF-1 is an 11.9-kDa fruiting body specific protein of the Ascomycetes hypogeous fungus Tuber borchii Vittad. found in aqueous extract and the hyphal cell wall. The tbf-1 gene codes a 12-amino acid N-terminal stretch not present in mature protein. This sequence does not match with any homologous signal sequences s...

journal_title：FEMS microbiology letters

authors： Palma F,Cerigini E,Stocchi V

更新日期：2007-07-01 00:00:00

abstract：:Biotype, genome, protein and plasmid profile diversity amongst 40 epidemiologically unrelated strains of Helicobacter pylori was studied. Strains were API Zym biotypes II, III and IV but most (87%) were biotype II. Four subsets of strains were defined on a combination of motility (56% positive) and cytotoxin productio...

journal_title：FEMS microbiology letters

authors： Owen RJ,Bickley J,Moreno M,Costas M,Morgan DR

更新日期：1991-04-15 00:00:00

abstract：:Cytoplasmic precursors of the peptidoglycan biosynthetic pathway were purified from vancomycin-treated, glycopeptide-sensitive and -resistant strains of Enterococcus faecium. Resistance was due to production of a modified precursor, UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-lactate, where lactate was identified on the basi...

journal_title：FEMS microbiology letters

authors： Messer J,Reynolds PE

更新日期：1992-07-01 00:00:00

abstract：:The coiled-coil protein DivIVA is a determinant of apical growth and hyphal branching in Streptomyces coelicolor. We have investigated the properties of this protein and the involvement of different domains in its essential function and subcellular targeting. In S. coelicolor cell extracts, DivIVA was present as large...

journal_title：FEMS microbiology letters

authors： Wang SB,Cantlay S,Nordberg N,Letek M,Gil JA,Flärdh K

更新日期：2009-08-01 00:00:00

abstract：:The growing demand to replace fossil fuels with renewable alternatives has generated an urgent and imminent global need to find new non-fossil sources. Sweet sorghum is widely recognized as a highly promising biomass energy crop with the particular potential to complement sugarcane for ethanol production. Our aim in t...

journal_title：FEMS microbiology letters

authors： Durán MA,Dantur K,Ruiz RM,Romero ER,Zossi S,Gusils C

更新日期：2018-07-01 00:00:00

abstract：:We adapted PCR-based signature-tagged mutagenesis (STM) to Pseudomonas aeruginosa. A collection of 1056 mutants was screened in a chronic lung infection rat model. Thirteen mutants were confirmed to be attenuated. Analysis revealed that these STM mutants represented transposon insertions into eight genes previously de...

journal_title：FEMS microbiology letters

authors： Lehoux DE,Sanschagrin F,Levesque RC

更新日期：2002-04-23 00:00:00

abstract：:Streptococcus bovis HC5 cultures released a broad spectrum lantibiotic, bovicin HC5, into the cell-fee culture supernatant after they reached stationary phase, but most of the antibacterial activity remained cell-associated. Cell-associated bovicin HC5 was more resistant to degradation by Pronase E than the cell-free ...

journal_title：FEMS microbiology letters

authors： Xavier BM,Houlihan AJ,Russell JB

更新日期：2008-06-01 00:00:00

abstract：:Viable counts and activities of sulfate-reducing bacteria were determined in the oral cavities of 12 healthy volunteers. Of these, 10 harboured viable sulfate-reducing bacteria populations. Six separate sites were sampled: the posterior tongue, anterior tongue, mid buccal mucosa, vestibular mucosa, supragingival plaqu...

journal_title：FEMS microbiology letters

authors： Willis CL,Gibson GR,Allison C,Macfarlane S,Holt JS

更新日期：1995-06-15 00:00:00

abstract：:We have studied the intraperoxisomal location of catalase in peroxisomes of methanol-grown Hansenula polymorpha by (immuno)cytochemical means. In completely crystalline peroxisomes, in which the crystalline matrix is composed of octameric alcohol oxidase (AO) molecules, most of the catalase protein is located in a nar...

journal_title：FEMS microbiology letters

authors： Keizer I,Roggenkamp R,Harder W,Veenhuis M

更新日期：1992-05-15 00:00:00

abstract：:Pneumocystis is a parasitic fungus causing pneumonia in immunosuppressed mammals and S-adenosylmethionine a key intermediary metabolite for all cells. Other than a species of Rickettsia bacteria and an aberrant strain of the protozoan Amoeba proteus, Pneumocystis is the only cell known unable to synthesize AdoMet; it ...

journal_title：FEMS microbiology letters

authors： Merali S,Clarkson AB Jr

更新日期：2004-08-15 00:00:00

abstract：:This study provides a novel qRT-PCR protocol for specific detection and proof of viability of Phytophthora in environmental samples based on differential accumulation of cox II transcripts. Chemical and physical treatments were tested for their ability to induce in vitro the accumulation of cytochrome oxidase genes en...

journal_title：FEMS microbiology letters

authors： Vannini A,Tomassini A,Bruni N,Vettraino AM

更新日期：2014-04-01 00:00:00

abstract：:Academic work at different career stages has changed and a broadened portfolio of expertise enables academics to adapt, maintain and advance their career. Development related to research activity is naturally driven by methodology and technology. Institutions and peers largely support development in the contexts of di...

journal_title：FEMS microbiology letters

authors： Fahnert B

更新日期：2015-10-01 00:00:00