Colony immunoblot assay for the detection of hemolysin BL enterotoxin producing Bacillus cereus.

abstract：:Bacillus cereus is becoming one of the more important causes of food poisoning in the industrialised world. It produces one emetic toxin and three different enterotoxins. The emetic toxin is a ring-shaped structure of three repeats of four amino and/or oxy acids: [D-O-Leu-D-Ala-L-O-Val-L-Val]3. This ring structure has...

journal_title：FEMS microbiology letters

authors： Granum PE,Lund T

更新日期：1997-12-15 00:00:00

abstract：:Biochemical responses to cold and osmotic stresses overlap because each decreases the availability of free water. Since RNA-binding proteins are known to accumulate following cold stress and play key roles in regulating transcription termination, the effect of osmotic stress on expression of RNA-binding proteins was e...

journal_title：FEMS microbiology letters

authors： Mori S,Castoreno A,Mulligan ME,Lammers PJ

更新日期：2003-10-24 00:00:00

abstract：:The emergence and dissemination of colistin resistance among Gram-negative bacteria is a global problem. We initiated a surveillance of colistin-resistant and -susceptible Escherichia coli in raw meats from chicken in Nepal. A total of 180 meat samples were collected; from these, 60 E. coli strains were isolated (33.3...

journal_title：FEMS microbiology letters

authors： Joshi PR,Thummeepak R,Leungtongkam U,Pooarlai R,Paudel S,Acharya M,Dhital S,Sitthisak S

更新日期：2019-10-01 00:00:00

abstract：:Using a glnII (encoding glutamine synthetase II) PCR selective screening, a Frankia ACN14a gene library clone was isolated. A derived glnII-hybridising 2.7-kb HindIII subclone was characterised. Identities of 95% and 93% were observed, respectively, with the corresponding Frankia CpI1 glnI and glnII regions. A variabl...

journal_title：FEMS microbiology letters

authors： Cournoyer B,Lavire C

更新日期：1999-08-01 00:00:00

abstract：:The benzimidazole fungicide thiophanate-methyl is commonly applied to control leaf mould of tomato caused by Cladosporium fulvum in China. In this study, 32 isolates of C. fulvum were examined for their sensitivities to thiophanate-methyl, and two benzimidazole-resistant (BenR) phenotypes BenR1 and BenR2 were identifi...

journal_title：FEMS microbiology letters

authors： Yan L,Chen J,Zhang C,Ma Z

更新日期：2008-01-01 00:00:00

abstract：:Atypical psychrophilic Aeromonas salmonicida isolates were obtained from farmed and wild fish in Northeastern North America. These bacteria were isolated between 1992 and 2001 and carried tetracycline resistance (Tc(r)) plasmids of approximately 58 kb. The nine isolates had plasmids which could be divided into four gr...

journal_title：FEMS microbiology letters

authors： Casas C,Anderson EC,Ojo KK,Keith I,Whelan D,Rainnie D,Roberts MC

更新日期：2005-01-01 00:00:00

abstract：:Levan fructotransferase (LFTase) from Arthrobacter ureafaciens K2032 was expressed with N-terminal fusion of a LacZ-derived secretion motif (TMITNSSSVP) using the lac promoter system in recombinant Escherichia coli JM109 [pUDF-A81]. In flask cultures, recombinant enzyme activity was detected in culture media, and sequ...

journal_title：FEMS microbiology letters

authors： Lee J,Saraswat V,Koh I,Song KB,Park YH,Rhee SK

更新日期：2001-02-20 00:00:00

abstract：:A lipopolysaccharide mutant of Leptospira interrogans (LaiMut) was obtained by growth in the presence of an agglutinating monoclonal antibody (mAb) against lipopolysaccharide. Agglutination reactions with anti-lipopolysaccharide mAbs and polyclonal antibodies showed that LaiMut had lost some serogroup Icterohaemorrhag...

journal_title：FEMS microbiology letters

authors： Zapata S,Trueba G,Bulach DM,Boucher D,Adler B,Hartskeerl R

更新日期：2010-08-01 00:00:00

abstract：:We have tested the expression of alkaline phosphatases in a mutant strain of Escherichia coli deficient in dipZ, a gene coding for a protein involved in cytochrome c biogenesis, and the isogenic wild-type strain. The yield of soluble and active human placental alkaline phosphatase was significantly reduced in the muta...

journal_title：FEMS microbiology letters

authors： Beck R,Crooke H,Jarsch M,Cole J,Burtscher H

更新日期：1994-12-01 00:00:00

abstract：:In Taiwan, leptospirosis is caused mainly by Leptospira santarosai serovar shermani. Suppression subtractive hybridization was employed to isolate DNA fragments present in pathogenic L. santarosai serovar shermani but absent in non-pathogenic L. biflexa serovar patoc. Analysis of 23 subtracted DNA clones revealed 25 g...

journal_title：FEMS microbiology letters

authors： Hsieh WJ,Pan MJ

更新日期：2004-06-01 00:00:00

abstract：:Streptomyces tendae Tü901 produces nikkomycins belonging to the nucleoside peptide antibiotics. Mutants defective in histidine catabolism were isolated and characterized with regard to their histidine ammonium-lyase activity and antibiotic synthesis. In the histidine ammonialyase-negative mutant hut-11 which was unimp...

journal_title：FEMS microbiology letters

authors： Roos U,Mattern S,Schrempf H,Bormann C

更新日期：1992-10-01 00:00:00

abstract：:Biological halogenation of aromatic compounds implies the generation of reducing equivalents in the form of e.g. NADH. Thermodynamic calculations show that coupling the halogenation step to a step in which the reducing equivalents are oxidized with a potent oxidant such as O2 or N2O makes the halogenation reaction the...

journal_title：FEMS microbiology letters

authors： Dolfing J

更新日期：1998-10-15 00:00:00

abstract：:A fast screening method was developed to assess the pathogenicity of a diverse collection of environmental and clinical Burkholderia cepacia complex isolates in the nematode Caenorhabditis elegans. The method was validated by comparison with the standard slow-killing assay. We observed that the pathogenicity of B. cep...

journal_title：FEMS microbiology letters

authors： Cardona ST,Wopperer J,Eberl L,Valvano MA

更新日期：2005-09-01 00:00:00

abstract：:Among the gram-negative microorganisms with probiotic properties, Escherichia coli strain Nissle 1917 (briefly EcN) is probably the most intensively investigated bacterial strain today. Since nearly 100 years, the EcN strain is used as the active pharmaceutical ingredient in a licensed medicinal product that is distri...

journal_title：FEMS microbiology letters

authors： Sonnenborn U

更新日期：2016-10-01 00:00:00

abstract：:A multiplex-polymerase chain reaction (PCR) assay based on one-step amplification and detection of two different mycobacterial genomic fragments was designed for differentiation of Mycobacterium bovis and Mycobacterium tuberculosis. The oligonucleotide primers were chosen from a 500-bp genomic fragment which is well c...

journal_title：FEMS microbiology letters

authors： Shah DH,Verma R,Bakshi CS,Singh RK

更新日期：2002-08-27 00:00:00

abstract：:Specific non-typhoid Salmonella serovars carry large virulence plasmids that promote sustained extra-intestinal infections. These plasmids all share a highly conserved 8-kb region containing the spv operon, consisting of the regulatory spvR locus and the four structural spvABCD genes. The SpvR protein belongs to the L...

journal_title：FEMS microbiology letters

authors： Guiney DG,Fang FC,Krause M,Libby S

更新日期：1994-11-15 00:00:00

abstract：:Tritrichomonas foetus is a venereal pathogen of cattle, which causes infertility, early embryonic death or abortion. In order to evaluate the potential trichomonicidal activity of benzimidazoles, the effects of thiabendazole, mebendazole and albendazole were analyzed on the multiplication, general morphology and ultra...

journal_title：FEMS microbiology letters

authors： Carvalho KP,Gadelha AP

更新日期：2007-10-01 00:00:00

abstract：:The microbial adhesion to hydrocarbons (MATH) test is one of the most common method to determine the hydrophobicity of cell surfaces. Despite its prevalence, no standard test parameters are used in literature, making a direct comparison of data almost impossible. Criticism also focuses on test parameters that may mask...

journal_title：FEMS microbiology letters

authors： Nachtigall C,Weber C,Rothenburger S,Jaros D,Rohm H

更新日期：2019-06-01 00:00:00

abstract：:CRISPR-Cas systems provide adaptive immunity against mobile genetic elements, but employment of this resistance mechanism is often reported with a fitness cost for the host. Whether or not CRISPR-Cas systems are important barriers for the horizontal spread of conjugative plasmids, which play a crucial role in the spre...

journal_title：FEMS microbiology letters

authors： van Sluijs L,van Houte S,van der Oost J,Brouns SJ,Buckling A,Westra ER

更新日期：2019-03-01 00:00:00

abstract：:The growing demand to replace fossil fuels with renewable alternatives has generated an urgent and imminent global need to find new non-fossil sources. Sweet sorghum is widely recognized as a highly promising biomass energy crop with the particular potential to complement sugarcane for ethanol production. Our aim in t...

journal_title：FEMS microbiology letters

authors： Durán MA,Dantur K,Ruiz RM,Romero ER,Zossi S,Gusils C

更新日期：2018-07-01 00:00:00

abstract：:Escherichia coli is the most common organism associated with asymptomatic bacteriuria (ABU) in humans. In contrast to uropathogenic E. coli (UPEC) that cause symptomatic urinary tract infection, very little is known about the mechanisms by which these strains colonize the urinary tract. Here, we have investigated the ...

journal_title：FEMS microbiology letters

authors： Hancock V,Ferrières L,Klemm P

更新日期：2007-02-01 00:00:00

abstract：:The aims of our research were to investigate the gene expression of the multidrug efflux transporter, CDR1 and the major drug facilitator superfamily transporter, MDR1 gene in azole drug-resistant Candida albicans and Candida glabrata clinical isolates recovered from vaginitis patients; and to identify hotspot mutatio...

journal_title：FEMS microbiology letters

authors： Looi CY,D' Silva EC,Seow HF,Rosli R,Ng KP,Chong PP

更新日期：2005-08-15 00:00:00

abstract：:An 18-kDa protein that occurs in Vibrio cholerae has been described as an in vivo and low-iron regulated outer membrane antigen. Monoclonal antibodies which recognized this antigen were protective as passive vaccines in the infant rabbit model of cholera disease. In this study, those monoclonal antibodies were used in...

journal_title：FEMS microbiology letters

authors： Sciortino CV Jr

更新日期：1993-07-15 00:00:00

abstract：:High resistance to class IIa bacteriocins in Listeria monocytogenes has been clearly linked to lack of expression of the mptACD operon, encoding the EIIt Man mannose PTS permease. Also, intermediate resistance has been associated with membrane phospholipid modifications in the spontaneous mutants L. monocytogenes B73-...

journal_title：FEMS microbiology letters

authors： Arous S,Dalet K,Héchard Y

更新日期：2004-09-01 00:00:00

abstract：:The genus Burkholderia comprises over 28 species and species-specific, recA-based polymerase chain reaction (PCR) tests are available for several species, but not for some soil-inhabiting species including B. fungorum. Previous analysis of several novel rhizospheric, environmental isolates belonging to the B. cepacia ...

journal_title：FEMS microbiology letters

authors： Chan CH,Stead DE,Coutts RH

更新日期：2003-07-15 00:00:00

abstract：:Nitrogen is a key nutrient for land plants and phytoplankton in terrestrial and aquatic ecosystems. The model alga Chlamydomonas reinhardtii can grow efficiently on several inorganic nitrogen sources (e.g. ammonium, nitrate, nitrite) as well as many amino acids. In this study, we show that Chlamydomonas is unable to u...

journal_title：FEMS microbiology letters

authors： Calatrava V,Hom EFY,Llamas Á,Fernández E,Galván A

更新日期：2018-04-01 00:00:00

abstract：:Transcription of the sigA and sigB genes of Brevibacterium lactofermentum, encoding the principal sigma factors of RNA polymerase, has been investigated by Northern blot and primer extension analysis. Northern hybridizations revealed that sigA is transcribed as a monocistronic mRNA of 1.7 kb and sigB gives two transcr...

journal_title：FEMS microbiology letters

authors： Oguiza JA,Marcos AT,Martín JF

更新日期：1997-08-01 00:00:00

abstract：:Enterococcal surface protein (Esp) is a cell wall-associated protein of Enterococcus faecalis that has been identified as a potential virulence factor. We used a mouse model to examine whether Esp facilitates intestinal colonization or translocation of E. faecalis to mesenteric lymph nodes. After clindamycin treatment...

journal_title：FEMS microbiology letters

authors： Pultz NJ,Shankar N,Baghdayan AS,Donskey CJ

更新日期：2005-01-15 00:00:00

abstract：:Two thermophilic bacteria, which are capable of growing on starch at 60-70 degrees C under anaerobic conditions, were isolated from a sugar refinery in Uelzen and from Solar lake in Israel. On the basis of their physiological characteristics they were identified as Clostridium thermohydrosulfuricum Uel 1 and C. thermo...

journal_title：FEMS microbiology letters

authors： Klingeberg M,Hippe H,Antranikian G

更新日期：1990-05-01 00:00:00

abstract：:Blocking glycolytic breakdown of glucose by inactivation of phosphoglucose isomerase (Pgi) in Escherichia coli led to a greatly reduced maximum specific growth rate. Examination of the operational catabolic pathways and their flux ratios using [U-(13)C(6)]glucose-labeling experiments and metabolic flux ratio analysis ...

journal_title：FEMS microbiology letters

authors： Canonaco F,Hess TA,Heri S,Wang T,Szyperski T,Sauer U

更新日期：2001-11-13 00:00:00