Abstract:
:High resistance to class IIa bacteriocins in Listeria monocytogenes has been clearly linked to lack of expression of the mptACD operon, encoding the EIIt Man mannose PTS permease. Also, intermediate resistance has been associated with membrane phospholipid modifications in the spontaneous mutants L. monocytogenes B73-V1 and B73-V2. We constructed a new mutant of L. monocytogenes that was interrupted in mpoA, and which also exhibited an intermediate resistance phenotype. The mpoABCD operon putatively encodes a PTS permease of the mannose family similar to that encoded by the mpt operon. In silico analysis indicated that mpo transcription might be dependent on sigma54. Our study demonstrated that the three intermediate resistant mutants have a slight decrease in mptACD expression, showing that the level of sensitivity is correlated to the level of mpt expression. We show a cross-regulation between mpo and mpt. In particular, the mpo mutant has a defect in mpt expression that possibly could explain its intermediate resistance phenotype.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Arous S,Dalet K,Héchard Ydoi
10.1016/j.femsle.2004.07.014keywords:
subject
Has Abstractpub_date
2004-09-01 00:00:00pages
37-41issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(04)00517-8journal_volume
238pub_type
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journal_title:FEMS microbiology letters
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