Loop-mediated isothermal amplification for the rapid detection of Salmonella.

abstract：:TraJ is an activator of the transfer (tra) operon in the F plasmid that counteracts H-NS silencing at the main transfer promoter (P(Y)). TraJ contains 226 aa (26 670 kDa), not 229 aa as reported previously, and forms homodimers. TraJ binds DNA containing P(Y)in vivo as demonstrated using a chromatin-immunoprecipitatio...

journal_title：FEMS microbiology letters

authors： Rodriguez-Maillard JM,Arutyunov D,Frost LS

更新日期：2010-09-01 00:00:00

abstract：:To study the pathogenicity of mouse cytomegalovirus (MCMV) and to identify virulence determinants, we have isolated and phenotypically characterised 25 temperature-sensitive (ts) mutants. Six of these (tsm9, tsm13, tsm20, tsm22, tsm28 and tsm30) failed to replicate in mice and were avirulent. Five mutants (tsm14, tsm1...

journal_title：FEMS microbiology letters

authors： Akel HM,Sweet C

更新日期：1993-11-01 00:00:00

abstract：:Five mutations in ccpA of Bacillus megaterium with impaired functions were analysed for carbon catabolite repression. The phenotypes support the hypothesis that CcpA assumes a PurR/LacI fold. The completely inactive mutants CcpA119GE and CcpA326am cause alterations which are incompatible with that fold. A mutation wit...

journal_title：FEMS microbiology letters

authors： Kraus A,Hillen W

更新日期：1997-08-01 00:00:00

abstract：:Bacterial populations produce persister cells that neither grow nor die in the presence of microbicidal antibiotics. Persisters are largely responsible for high levels of biofilm tolerance to antimicrobials, but virtually nothing was known about their biology. Tolerance of Escherichia coli to ampicillin and ofloxacin ...

journal_title：FEMS microbiology letters

authors： Keren I,Kaldalu N,Spoering A,Wang Y,Lewis K

更新日期：2004-01-15 00:00:00

abstract：:Aceticlastic methanogens metabolize acetate to methane and carbon dioxide. The central metabolism and the electron transport chains of these organisms have already been investigated. However, no particular attention has been paid to the mechanism by which acetate enters the archaeal cell. In our study we investigated ...

journal_title：FEMS microbiology letters

authors： Welte C,Kröninger L,Deppenmeier U

更新日期：2014-10-01 00:00:00

abstract：:Dissimilatory metal-reducing bacteria (DMRB), such as Shewanella oneidensis MR-1, are of great interest for their importance in the biogeochemical cycling of metals and utility in biotechnological processes, such as bioremediation and microbial fuel cells. To identify genes necessary for metal reduction, this study co...

journal_title：FEMS microbiology letters

authors： Kouzuma A,Hashimoto K,Watanabe K

更新日期：2012-01-01 00:00:00

abstract：:A lipopolysaccharide mutant of Leptospira interrogans (LaiMut) was obtained by growth in the presence of an agglutinating monoclonal antibody (mAb) against lipopolysaccharide. Agglutination reactions with anti-lipopolysaccharide mAbs and polyclonal antibodies showed that LaiMut had lost some serogroup Icterohaemorrhag...

journal_title：FEMS microbiology letters

authors： Zapata S,Trueba G,Bulach DM,Boucher D,Adler B,Hartskeerl R

更新日期：2010-08-01 00:00:00

abstract：:Fusarium graminearum was grown on four lignocellulosic substrates (corn cobs, wheat bran, hop cell walls, and birchwood) and glucose as the sole carbon source. Proteomic studies performed on the resulting enzymatic cocktails highlighted a great diversity in the number and type of proteins secreted. The cell wall-degra...

journal_title：FEMS microbiology letters

authors： Debeire P,Delalande F,Habrylo O,Jeltsch JM,Van Dorsselaer A,Phalip V

更新日期：2014-06-01 00:00:00

abstract：:Reduction of D-fructose to D-mannitol by whole-cell biotransformation with recombinant resting cells of Corynebacterium glutamicum ATCC13032 requires the coexpression of mdh and fdh, which encode mannitol and formate dehydrogenases, respectively. However, d-mannitol formation is limited by the uptake of d-fructose in ...

journal_title：FEMS microbiology letters

authors： Bäumchen C,Krings E,Bringer S,Eggeling L,Sahm H

更新日期：2009-01-01 00:00:00

abstract：:The purpose of this study was to generate highly specific serological reagents for the quantitative identification of Actinomyces naeslundii in clinical samples, in particular dental plaque. Balb/c mice were immunized with pasteurized human A. naeslundii strains representing different genospecies and serotypes. Ten hy...

journal_title：FEMS microbiology letters

authors： Thurnheer T,Guggenheim B,Gmür R

更新日期：1997-05-15 00:00:00

abstract：:In this report we describe and characterize two oligomer probes that are broadly homologous to conserved eubacterial 16S ribosomal RNA (rRNA) sequences not present in human 18 rRNA or human mitochondrial 12S rRNA. One or both of the probes can detect all of 23 phylogenetically diverse eubacterial nucleic acids against...

journal_title：FEMS microbiology letters

authors： Chen K,Neimark H,Rumore P,Steinman CR

更新日期：1989-01-01 00:00:00

abstract：:Sixteen of the 18 Vibrio cholerae rough strains isolated from hospitalised diarrhoea patients were found to contain O1 serotype-specific (wbe) genes and all currently known virulence genes. Expression of the regulatory element ToxR was evident in these strains. Cholera toxin production ability of the rough strains was...

journal_title：FEMS microbiology letters

authors： De K,Ramamurthy T,Faruque SM,Yamasaki S,Takeda Y,Nair GB,Nandy RK

更新日期：2004-03-12 00:00:00

abstract：:Photorhabdus (Enterobacteriaceae) bacteria are pathogenic to insects and mutualistic with entomopathogenic Heterorhabditis nematodes. Photorhabdus luminescens subsp. akhurstii LN2, associated with Heterorhabditis indica LN2, shows nematicidal activity against H. bacteriophora H06 infective juveniles (IJs). In the pres...

journal_title：FEMS microbiology letters

authors： Qiu X,Wu C,Cao L,Ehlers RU,Han R

更新日期：2016-03-01 00:00:00

abstract：:Porphyromonas gingivalis produces a trypsin-like enzyme, Protease I, which is thought to be an important virulence determinant of the organism in adult periodontal disease. Protease I is transiently inhibited by physiological inhibitors of human thrombin. The aim of the present work was to establish whether Protease I...

journal_title：FEMS microbiology letters

authors： Curtis MA,Macey M,Slaney JM,Howells GL

更新日期：1993-06-15 00:00:00

abstract：:The hybrid Pseudomonas cepacia strain JHR22 was tested for its ability to degrade Aroclor 1221 in soil. The influence of supplements--mineral salts and trace elements--on the degradation was investigated. Disappearance of Aroclor 1221 congeners, occurrence of metabolites, and release of chloride were measured under di...

journal_title：FEMS microbiology letters

authors： Havel J,Reineke W

更新日期：1993-04-01 00:00:00

abstract：:As part of the ongoing sequencing of the complete Salmonella typhimurium LT2 genome, a partly ordered set of 416 lambda clones has been developed, representing over 90% of the genome. The average insert size is 17 kb. Sequences were obtained from both ends of each clone in this set. A total of over 600 kb of sequence ...

journal_title：FEMS microbiology letters

authors： Wong RM,Wong KK,Benson NR,McClelland M

更新日期：1999-04-15 00:00:00

abstract：:To investigate the auto-ecology of a strain of Gigaspora margarita in a commercial inoculum, we found a pair of PCR primers amplifying a sequence of 235 bp diagnostic of the isolate. We designed an oligonucleotide probe based on the DNA sequence. The combination of PCR and the probing successfully detected the diagnos...

journal_title：FEMS microbiology letters

authors： Yokoyama K,Tateishi T,Marumoto T,Saito M

更新日期：2002-07-02 00:00:00

abstract：:The anr gene of Pseudomonas aeruginosa encodes a transcriptional regulator of anaerobic gene expression, homologous to the Fnr protein of Escherichia coli. We report here that Anr has a role in regulating the activity of the aerobic respiratory chain of P. aeruginosa. Strains with internal deletions in their anr gene ...

journal_title：FEMS microbiology letters

authors： Ray A,Williams HD

更新日期：1997-11-15 00:00:00

abstract：:In Bacillus subtilis, the yoxA and dacC genes were proposed to form an operon. The yoxA gene was overexpressed in Escherichia coli and its product fused to a polyhistidine tag was purified. An aldose-1-epimerase or mutarotase activity was measured with the YoxA protein that we propose to rename as GalM by analogy with...

journal_title：FEMS microbiology letters

authors： Duez C,Zervosen A,Teller N,Melkonian R,Banzubazé E,Bouillenne F,Luxen A,Frère JM

更新日期：2009-11-01 00:00:00

abstract：:We report here improvements to the growth media and fermentation conditions which result in a substantial increase of Helicobacter pylori growth and in the enhanced production of vacuolating cytotoxin. Addition of glucose to the medium resulted in the increase of cell yield, cell viability and a significant improvemen...

journal_title：FEMS microbiology letters

authors： Marchini A,Massari P,Manetti R,Olivieri R

更新日期：1994-11-15 00:00:00

abstract：:Streptomyces coelicolor A3(2) produces an antibiotic, actinorhodin, which belongs to the aromatic polyketides and which can function as an acid/base indicator. Its production results in the death of microorganisms in the vicinity of S. coelicolor A3(2), and this phenomenon can be used in concert with biopesticides. Th...

journal_title：FEMS microbiology letters

authors： Lee Y,Kim K,Suh JW,Rhee S,Lim Y

更新日期：2007-01-01 00:00:00

abstract：:The malate dehydrogenase (MDH; EC 1.1.1.37; L-malate-NAD(+)-oxidoreductase) activities of truffles of the genus Tuber (Tuber melanosporum Vittad., Tuber brumale Vittad., Tuber aestivum Vittad., Tuber magnatum Pico, Tuber rufum Pico) have been characterized with regard to the K(m) and V(max) values in the direct and re...

journal_title：FEMS microbiology letters

authors： Zarivi O,Cesare P,Aimola P,Ragnelli AM,Scirri C,Cimini A,Bonfigli A,Pacioni G,Miranda M

更新日期：2000-04-15 00:00:00

abstract：:Eikenella corrodens 33EK(L), a clinical isolate, was assayed for its ability to utilise amino acids as substrates in the reduction of nitrate to nitrite. The metabolism of proline, glutamate, serine and glutamine was found to result in relatively high rates of nitrate reduction. The ability of cells to metabolise thes...

journal_title：FEMS microbiology letters

authors： Gully NJ,Rogers AH

更新日期：1996-06-01 00:00:00

abstract：:We identified a novel type-III staphylococcal cassette chromosome mec (SCCmec) element carried by eight methicillin-resistant Staphylococcus aureus (MRSA) strains from different wards and patients in an Indian hospital. Although the pulsed-field gel electrophoresis pattern and spa types of eight strains were identical...

journal_title：FEMS microbiology letters

authors： Arakere G,Nadig S,Ito T,Ma XX,Hiramatsu K

更新日期：2009-03-01 00:00:00

abstract：:Lyme disease is a multisystemic disorder caused by Borrelia burgdorferi, an invasive spirochete. B. burgdorferi has a predilection for collagenous tissue and one major clinical manifestation of the disease is arthritis. We have identified a collagenolytic activity in B. burgdorferi detergent lysates using iodinated ge...

journal_title：FEMS microbiology letters

authors： Grab DJ,Kennedy R,Philipp MT

更新日期：1996-10-15 00:00:00

abstract：:We found the presence of DNA sequence which shows sequence similarity to the class IV chitin synthase gene (CHS3) of Saccharomyces cerevisiae in the genome of 14 Rhizopus species which belong to zygomycetes. We cloned a gene (chs3), which might correspond to one of these homologous sequences, from Rhizopus oligosporus...

journal_title：FEMS microbiology letters

authors： Motoyama T,Horiuchi H,Ohta A,Yamaguchi I,Takagi M

更新日期：1998-12-01 00:00:00

abstract：:Periodic evolution of H(2)S during aerobic chemostat culture of Saccharomyces cerevisiae resulted in ultradian metabolic oscillation via periodic inhibition of respiratory activity. To understand the nature of periodic H(2)S evolution, we investigated whether oxidative stress is associated with H(2)S production. The c...

journal_title：FEMS microbiology letters

authors： Kwak WJ,Kwon GS,Jin I,Kuriyama H,Sohn HY

更新日期：2003-02-14 00:00:00

abstract：:parD and chpA are homologous conditional killer systems of plasmid and chromosomal origin, respectively, encoding a killer protein (Kid and ChpAK) and an antidote (Kis and ChpAI). Here it is shown that these systems can functionally interact. A multicopy chpA recombinant partially complements two mutations in the anti...

journal_title：FEMS microbiology letters

authors： Santos-Sierra S,Giraldo R,Díaz-Orejas R

更新日期：1997-07-01 00:00:00

abstract：:Rhodopirellula baltica (strain SH 1T) is a free-living marine representative of the phylogenetically independent and environmentally relevant phylum Planctomycetes. Little is known about the regulatory strategies of free-living bacteria with large (7.15 Mb) genomes. Therefore, a consistent, quantitative and qualitativ...

journal_title：FEMS microbiology letters

authors： Lombardot T,Bauer M,Teeling H,Amann R,Glöckner FO

更新日期：2005-01-01 00:00:00

abstract：:Bradyrhizobium japonicum has two types of flagella. One has thin filaments consisting of the 33-kDa flagellins FliCI and FliCII (FliCI-II) and the other has thick filaments consisting of the 65-kDa flagellins FliC1, FliC2, FliC3, and FliC4 (FliC1-4). To investigate the roles of each flagellum in competition for nodula...

journal_title：FEMS microbiology letters

authors： Althabegoiti MJ,Covelli JM,Pérez-Giménez J,Quelas JI,Mongiardini EJ,López MF,López-García SL,Lodeiro AR

更新日期：2011-06-01 00:00:00