Persister cells and tolerance to antimicrobials.

Abstract:

:Bacterial populations produce persister cells that neither grow nor die in the presence of microbicidal antibiotics. Persisters are largely responsible for high levels of biofilm tolerance to antimicrobials, but virtually nothing was known about their biology. Tolerance of Escherichia coli to ampicillin and ofloxacin was tested at different growth stages to gain insight into the nature of persisters. The number of persisters did not change in lag or early exponential phase, and increased dramatically in mid-exponential phase. Similar dynamics were observed with Pseudomonas aeruginosa (ofloxacin) and Staphylococcus aureus (ciprofloxacin and penicillin). This shows that production of persisters depends on growth stage. Maintaining a culture of E. coli at early exponential phase by reinoculation eliminated persisters. This suggests that persisters are not at a particular stage in the cell cycle, neither are they defective cells nor cells created in response to antibiotics. Our data indicate that persisters are specialized survivor cells.

journal_name

FEMS Microbiol Lett

authors

Keren I,Kaldalu N,Spoering A,Wang Y,Lewis K

doi

10.1016/S0378-1097(03)00856-5

keywords:

subject

Has Abstract

pub_date

2004-01-15 00:00:00

pages

13-8

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378109703008565

journal_volume

230

pub_type

杂志文章
  • Growth of Salmonella enteritidis and S. pullorum on Hektoen agar and the expression of lipopolysaccharide or flagella.

    abstract::Growth of strains of Salmonella enteritidis and Salmonella pullorum on Hektoen agar has been reported to influence the expression of long-chain lipopolysaccharide and motility respectively. In this study we used a panel of strains of S. enteritidis and S. pullorum to investigate these phenomena. Culture on Hektoen aga...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13043.x

    authors: Chart H,Rowe B

    更新日期:1998-06-15 00:00:00

  • Biotransformation of trinitrotoluene by Citrobacter sp. YC4 and evaluation of its cyto-toxicological effects.

    abstract::Trinitrotoluene (TNT) is an explosive chemical generally used for military, civil and industrial purposes. Therefore, TNT residue can be found in soil and ground water as an environmental pollutant. The environmental control of TNT pollution has become a critical issue because of its potential toxicity and carcinogeni...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx256

    authors: Kao CM,Wei SF,Chen SC,Yao CL,Ma C,Chien CC

    更新日期:2018-01-01 00:00:00

  • Marine phage genomics: the tip of the iceberg.

    abstract::Marine viruses are the most abundant biological entity in the oceans, the majority of which infect bacteria and are known as bacteriophages. Yet, the bulk of bacteriophages form part of the vast uncultured dark matter of the microbial biosphere. In spite of the paucity of cultured marine bacteriophages, it is known th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fnw158

    authors: Perez Sepulveda B,Redgwell T,Rihtman B,Pitt F,Scanlan DJ,Millard A

    更新日期:2016-08-01 00:00:00

  • Anaerobic degradation of protocatechuate (3,4-dihydroxybenzoate) by Thauera aromatica strain AR-1.

    abstract::The denitrifying bacterium Thauera aromatica strain AR-1 grows anaerobically with protocatechuate (3,4-dihydroxybenzoate (DHB)) as sole energy and carbon source. This bacterium harbors two distinct pathways for degradation of aromatic compounds, the benzoyl-coenzyme A (CoA) pathway for benzoate degradation and the hyd...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11257.x

    authors: Philipp B,Kemmler D,Hellstern J,Gorny N,Caballero A,Schink B

    更新日期:2002-06-18 00:00:00

  • ATP/ADP alteration as a sign of the oxidative stress development in Escherichia coli cells under antibiotic treatment.

    abstract::The extensively discussed idea of oxidative stress development under antibiotic treatment was confirmed using an antioxidant gene expression (soxRS-, oxyR-regulon) approach, including microaerobic cultivation conditions. The killing action of antibiotics and their ability to cause peroxide oxidative stress in Escheric...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12405

    authors: Akhova AV,Tkachenko AG

    更新日期:2014-04-01 00:00:00

  • A structurally novel staphylococcal protein A from the V8 strain.

    abstract::Protein A from the Staphylococcus aureus strain V8 has a molecular mass about 8000 Da less than that of known proteins A. The corresponding gene was cloned and expressed in Escherichia coli. Sequence analysis of this structurally new protein A revealed that it lacked an IgG-binding domain (58 amino acids), and that it...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90554-2

    authors: Finck-Barbançon V,Prevost G,Mazurier I,Piemont Y

    更新日期:1992-02-01 00:00:00

  • Differential expression of putative drug resistance genes in Mycobacterium tuberculosis clinical isolates.

    abstract::Understanding drug resistance in Mycobacterium tuberculosis requires an integrated analysis of strain lineages, mutations and gene expression. Previously, we reported the differential expression of esxG, esxH, infA, groES, rpmI, rpsA and lipF genes in a sensitive M. tuberculosis strain and in a multidrug-resistant cli...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv194

    authors: González-Escalante L,Peñuelas-Urquides K,Said-Fernández S,Silva-Ramírez B,Bermúdez de León M

    更新日期:2015-12-01 00:00:00

  • Rapid identification of marine bioluminescent bacteria by amplified 16S ribosomal RNA gene restriction analysis.

    abstract::To rapidly identify natural isolates of marine bioluminescent bacteria, we developed amplified ribosomal DNA restriction analysis (ARDRA) methods. ARDRA, which is based on the restriction patterns of 16S rRNA gene digested with five enzymes (EcoRI, DdeI, HhaI, HinfI, RsaI), clearly distinguished the 14 species of mari...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00129.x

    authors: Kita-Tsukamoto K,Wada M,Yao K,Kamiya A,Yoshizawa S,Uchiyama N,Kogure K

    更新日期:2006-03-01 00:00:00

  • Concomitant expression of E. coli cytosine deaminase and uracil phosphoribosyltransferase improves the cytotoxicity of 5-fluorocytosine.

    abstract::The prodrug activation system formed by the E. coli codA gene encoding cytosine deaminase (CD) and 5-fluorocytosine (5-FC) developed for selective cancer chemotherapy suffers from a sensitivity limitation in many tumour cells. In an attempt to improve the CD/5-FC suicide association, we combined the E. coli upp gene e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13205.x

    authors: Tiraby M,Cazaux C,Baron M,Drocourt D,Reynes JP,Tiraby G

    更新日期:1998-10-01 00:00:00

  • Induction of a viable but non-culturable state in Salmonella Typhimurium by thermosonication and factors affecting resuscitation.

    abstract::The objective of this work was to analyze the effects of thermosonication (TS) on induction of a viable but non-culturable (VBNC) state in Salmonella Typhimurium and to examine incubation factors affecting subsequent resuscitation of cells. A TS treatment of 380 W at 53°C for 30 min induced the VBNC state in S. Typhim...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx249

    authors: Liao H,Jiang L,Zhang R

    更新日期:2018-02-01 00:00:00

  • Astaxanthin formation in the marine photosynthetic bacterium Rhodovulum sulfidophilum expressing crtI, crtY, crtW and crtZ.

    abstract::This study reports the production of astaxanthin in the photosynthetic bacterium Rhodovulum sulfidophilum, which has adequate precursor pools and storage capabilities for heterologous carotenoid production. Chemical mutagenesis was carried out using ethylmethane sulfonate to produce mutants with a modified carotenoid ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00471.x

    authors: Mukoyama D,Takeyama H,Kondo Y,Matsunaga T

    更新日期:2006-12-01 00:00:00

  • Sesquiterpene farnesol as a competitive inhibitor of lipase activity of Staphylococcus aureus.

    abstract::Staphylococcus aureus lipase (SAL) is known to possess broad substrate specificity for triacylglycerides. We found that a sub-minimum inhibitory concentration of farnesol (1000 mg L(-1)) inhibits this lipase activity on a Mueller-Hinton agar containing 1% Tween substrates. A quantitative lipase assay using p-nitrophen...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00772.x

    authors: Kuroda M,Nagasaki S,Ito R,Ohta T

    更新日期:2007-08-01 00:00:00

  • Use of intersimple sequence repeats markers to develop strain-specific SCAR markers for Lentinula edodes.

    abstract::Although Lentinula edodes is the second most important cultivated mushroom worldwide, most industrially cultivated strains have been identified only through traditional phenotypic analysis. Here, we report for the first time the use of sequence characterized amplified region (SCAR) markers for strain differentiation. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00145.x

    authors: Qin LH,Tan Q,Chen MJ,Pan YJ

    更新日期:2006-04-01 00:00:00

  • Modified peptidoglycan precursors produced by glycopeptide-resistant enterococci.

    abstract::Cytoplasmic precursors of the peptidoglycan biosynthetic pathway were purified from vancomycin-treated, glycopeptide-sensitive and -resistant strains of Enterococcus faecium. Resistance was due to production of a modified precursor, UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-lactate, where lactate was identified on the basi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90608-q

    authors: Messer J,Reynolds PE

    更新日期:1992-07-01 00:00:00

  • Amino acid and nucleotide sequence, adjacent genes, and heterologous expression of hiracin JM79, a sec-dependent bacteriocin produced by Enterococcus hirae DCH5, isolated from Mallard ducks (Anas platyrhynchos).

    abstract::The primary structure of a bacteriocin produced by Enterococcus hirae DCH5 was determined by combined amino acid and DNA sequencing. Nucleotide analysis of a 2838-bp DNA fragment of E. hirae DCH5 revealed five putative ORFs. The first orf (hirJM79) encodes a 74-amino-acid peptide containing an N-terminal signal peptid...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00673.x

    authors: Sánchez J,Diep DB,Herranz C,Nes IF,Cintas LM,Hernández PE

    更新日期:2007-05-01 00:00:00

  • Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean.

    abstract::Bradyrhizobium japonicum has two types of flagella. One has thin filaments consisting of the 33-kDa flagellins FliCI and FliCII (FliCI-II) and the other has thick filaments consisting of the 65-kDa flagellins FliC1, FliC2, FliC3, and FliC4 (FliC1-4). To investigate the roles of each flagellum in competition for nodula...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02280.x

    authors: Althabegoiti MJ,Covelli JM,Pérez-Giménez J,Quelas JI,Mongiardini EJ,López MF,López-García SL,Lodeiro AR

    更新日期:2011-06-01 00:00:00

  • Cloning and expression in Escherichia coli of dextranase genes from Bacteroides thetaiotaomicron.

    abstract::A genomic bank was constructed in Escherichia coli HB101, consisting of DNA fragments from Bacteroides thetaiotaomicron strain 489 inserted within the vector pBR322. By screening on complex medium containing blue dextran, 10 stable dextranase-positive (Dex+) clones were isolated. Seven groups of Dex+ inserts were iden...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(91)90368-k

    authors: Joncquiert JC,Béchet M,Tierny Y,Courtois J,Dubourguier HC,Guillaume JB

    更新日期:1991-12-01 00:00:00

  • An Arf-GAP promotes endocytosis and hyphal growth of Ashbya gossypii.

    abstract::The ADP-ribosylation factor (ARF) family of GTPases are highly conserved from yeast to human and regulate vesicle budding. Sec7 domain containing proteins stimulate the guanine nucleotide exchange on Arf proteins, while ARF-GTPase activating proteins stimulate the hydrolysis of GTP. Since vesicle trafficking is import...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx240

    authors: Oscarsson T,Walther A,Lengeler KB,Wendland J

    更新日期:2017-12-29 00:00:00

  • Characterization of dextran-producing Weissella strains isolated from sourdoughs and evidence of constitutive dextransucrase expression.

    abstract::The study of exopolysaccharide production by heterofermentative sourdough lactic acid bacteria has shown that Weissella strains isolated from sourdoughs produce linear dextrans containing α-(1→6) glucose residues with few α-(1→3) linkages from sucrose. In this study, several dextran-producing strains, Weissella cibari...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.02067.x

    authors: Bounaix MS,Robert H,Gabriel V,Morel S,Remaud-Siméon M,Gabriel B,Fontagné-Faucher C

    更新日期:2010-10-01 00:00:00

  • Roundup Ready soybean gene concentrations in field soil aggregate size classes.

    abstract::Roundup Ready (RR) soybeans containing recombinant Agrobacterium spp. CP4 5-enol-pyruvyl-shikimate-3-phosphate synthase (cp4 epsps) genes tolerant to the herbicide glyphosate are extensively grown worldwide. The concentration of recombinant DNA from RR soybeans in soil aggregates was studied due to the possibility of ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01449.x

    authors: Levy-Booth DJ,Gulden RH,Campbell RG,Powell JR,Klironomos JN,Pauls KP,Swanton CJ,Trevors JT,Dunfield KE

    更新日期:2009-02-01 00:00:00

  • The amo operon in marine, ammonia-oxidizing gamma-proteobacteria.

    abstract::While there is an extensive database of genes encoding ammonia monooxygenase (amo) found in the ammonia-oxidizing beta-proteobacteria, few amo sequences are available representing the gamma-proteobacteria. We sequenced the complete amo operon (amoCAB) for Nitrosococcus oceani (ATCC 19707), a marine, autotrophic, ammon...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb08773.x

    authors: Alzerreca JJ,Norton JM,Klotz MG

    更新日期:1999-11-01 00:00:00

  • NtrBC-dependent expression from the Rhizobium meliloti dctA promoter in Escherichia coli.

    abstract::Effects of the two-component sensor-regulator pairs DctBD and NtrBC upon the expression of a dctA::phoA fusion from Rhizobium meliloti were determined under excess and limiting nitrogen concentrations in Escherichia coli. Results indicated that NtrBC affected transcription from the dctA promoter on a number of regulat...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07531.x

    authors: Allaway D,Boesten B,O'Gara F

    更新日期:1995-05-15 00:00:00

  • Molybdate-dependent expression of dimethylsulfoxide reductase in Rhodobacter capsulatus.

    abstract::Expression of the dimethylsulfoxide respiratory (dor) operon of Rhodobacter is regulated by oxygen, light intensity and availability of substrate. Since dimethylsulfoxide reductase contains a pterin molybdenum cofactor, the role of molybdate in the regulation of dor operon expression was investigated. In this report w...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09287.x

    authors: Solomon PS,Shaw AL,Young MD,Leimkuhler S,Hanson GR,Klipp W,McEwan AG

    更新日期:2000-09-15 00:00:00

  • Obligate phototrophy in cyanobacteria: more than a lack of sugar transport.

    abstract::DNA hybridization using the Synechocystis PCC6803 glucose transporter gene, glcP, revealed a single homologous region in two facultative photoautotrophic strains out of three tested, and none in three obligate autotrophs. In one of the latter, Synechococcus PCC7942, integration of glcP into the chromosome resulted in ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb12959.x

    authors: Zhang CC,Jeanjean R,Joset F

    更新日期:1998-04-15 00:00:00

  • The triazine hydrolase gene trzN from Nocardioides sp. strain C190: cloning and construction of gene-specific primers.

    abstract::Using oligonucleotides derived from the N-terminal sequence of a triazine hydrolase from Nocardioides sp. strain C190, two DNA fragments containing trzN were cloned into Escherichia coli and their nucleotide sequences were determined. The 456-amino acid polypeptide predicted from the 1356-bp trzN ORF displayed signifi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb10989.x

    authors: Mulbry WW,Zhu H,Nour SM,Topp E

    更新日期:2002-01-02 00:00:00

  • Isolation of insertion elements from gram-positive Brevibacterium, Corynebacterium and Rhodococcus strains using the Bacillus subtilis sacB gene as a positive selection marker.

    abstract::The sacB gene of Bacillus subtilis was successfully applied in various Arthrobacter, Brevibacterium, Corynebacterium and Rhodococcus strains for the isolation of transposable elements. Three different insertion sequence (IS) elements entrapped in sacB were isolated. The IS elements IS-Bl and IS-Cg isolated from Brevib...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07381.x

    authors: Jäger W,Schäfer A,Kalinowski J,Pühler A

    更新日期:1995-02-01 00:00:00

  • Isolation and partial characterization of a compound with siderophore activity from Vibrio parahaemolyticus.

    abstract::A compound with siderophore activity was purified by successive column and thin layer chromatographic procedures from Dowex 1 x 8 extracts of culture supernatants of Vibrio parahaemolyticus AQ 3354. The strain synthesized the compound in culture media containing less than 2 microM added FeCl3. Hydrolysis of the compou...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90605-n

    authors: Yamamoto S,Fujita Y,Okujo N,Minami C,Matsuura S,Shinoda S

    更新日期:1992-07-01 00:00:00

  • Cell-fies: sharing microbiology with global audiences through Instagram.

    abstract::For the dissemination of microbiology and science in general, social media is an underutilized but increasingly powerful tool, and one that transcends traditional avenues for scientific outputs. In order to reach and potentially inspire a deeply interested and general public, scientists should engage with platforms su...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz205

    authors: Hines HN

    更新日期:2019-08-01 00:00:00

  • Clonal turnover of enterohemorrhagic Escherichia coli O157:H7 in experimentally infected cattle.

    abstract::A total of 401 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates from two experimentally infected calves were analyzed using molecular biological methods. Genetic differences detected by pulsed-field gel electrophoresis were observed between the inoculated and recovered strains as early as 1 day post inoculat...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb08994.x

    authors: Akiba M,Sameshima T,Nakazawa M

    更新日期:2000-03-01 00:00:00

  • Different Mesorhizobium species associated with Caragana carry similar symbiotic genes and have common host ranges.

    abstract::Fourteen strains representing 11 Caragana-nodulating Mesorhizobium genomic species were identified as representing Mesorhizobium amorphae, Mesorhizobium huakuii, Mesorhizobium septentrionale and groups related to Mesorhizobium plurifarium, Mesorhizobium temperatum, Mesorhizobium tianshanense and Mesorhizobium mediterr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01167.x

    authors: Chen WF,Guan SH,Zhao CT,Yan XR,Man CX,Wang ET,Chen WX

    更新日期:2008-06-01 00:00:00