The effects of mutation of the anr gene on the aerobic respiratory chain of Pseudomonas aeruginosa.

Abstract:

:The anr gene of Pseudomonas aeruginosa encodes a transcriptional regulator of anaerobic gene expression, homologous to the Fnr protein of Escherichia coli. We report here that Anr has a role in regulating the activity of the aerobic respiratory chain of P. aeruginosa. Strains with internal deletions in their anr gene had lowered levels of membrane bound cytochromes whilst the activity of the cytochrome c oxidase, cytochrome co (likely to be a cytochrome cbb3-type oxidase), and the cyanide-insensitive respiratory pathway was markedly higher than in the wild-type strains. These data, and the finding that provision of multiple copies of the anr gene led to severe repression of these respiratory activities, suggest that Anr is a repressor of aerobic respiratory pathways and possibly the terminal oxidases themselves. In contrast, Anr activated cytochrome c peroxidase, a respiratory chain linked enzyme induced under low oxygen conditions.

journal_name

FEMS Microbiol Lett

authors

Ray A,Williams HD

doi

10.1111/j.1574-6968.1997.tb12732.x

subject

Has Abstract

pub_date

1997-11-15 00:00:00

pages

227-32

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(97)00429-1

journal_volume

156

pub_type

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