Spiroethers of German chamomile inhibit production of aflatoxin G and trichothecene mycotoxin by inhibiting cytochrome P450 monooxygenases involved in their biosynthesis.

Abstract:

:The essential oil of German chamomile showed specific inhibition toward aflatoxin G(1) (AFG(1)) production, and (E)- and (Z)-spiroethers were isolated as the active compounds from the oil. The (E)- and (Z)-spiroethers inhibited AFG(1) production of Aspergillus parasiticus with inhibitory concentration 50% (IC(50)) values of 2.8 and 20.8 microM, respectively, without inhibiting fungal growth. Results of an O-methylsterigmatocystin (OMST) conversion study indicated that the spiroethers specifically inhibited the OMST to AFG(1) pathway. A cytochrome P450 monooxygenase, CYPA, is known as an essential enzyme for this pathway. Because CYPA has homology with TRI4, a key enzyme catalyzing early steps in the biosynthesis of trichothecenes, the inhibitory actions of the two spiroethers against TRI4 reactions and 3-acetyldeoxynivalenol (3-ADON) production were tested. (E)- and (Z)-spiroethers inhibited the enzymatic activity of TRI4 dose-dependently and interfered with 3-ADON production by Fusarium graminearum, with IC(50) values of 27.1 and 103 microM, respectively. Our results suggest that the spiroethers inhibited AFG(1) and 3-ADON production by inhibiting CYPA and TRI4, respectively.

journal_name

FEMS Microbiol Lett

authors

Yoshinari T,Yaguchi A,Takahashi-Ando N,Kimura M,Takahashi H,Nakajima T,Sugita-Konishi Y,Nagasawa H,Sakuda S

doi

10.1111/j.1574-6968.2008.01195.x

subject

Has Abstract

pub_date

2008-07-01 00:00:00

pages

184-90

issue

2

eissn

0378-1097

issn

1574-6968

pii

FML1195

journal_volume

284

pub_type

杂志文章
  • Construction and evaluation of a cea-lacZ gene fusion for the detection of environmental mutagens and carcinogens.

    abstract::The cea-kil operon of the ColE1 plasmid is negatively regulated by the LexA-repressor and therefore, it is under the control of SOS regulation. We constructed a gene fusion between the cea and lacZ genes. Expression of the translational fusion can be easily detected by monitoring the levels of beta-galactosidase. Sinc...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(91)90232-y

    authors: Schumann W,Songür N,Miltenburger HG

    更新日期:1991-07-01 00:00:00

  • Hydroxyurea inhibits intracellular Toxoplasma gondii multiplication.

    abstract::Tachyzoites of Toxoplasma gondii multiply within the parasitophorous vacuole (PV) until the lysis of the host cell. This study was undertaken to evaluate the effect of hydroxyurea (a specific drug that arrests cell division at G1/S phase) on the multiplication of T. gondii tachyzoites in infected Vero cells. Infected ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/s0378-1097(00)00074-4

    authors: de Melo EJ,Mayerhoffer RO,de Souza W

    更新日期:2000-04-01 00:00:00

  • Horizontal transfer of nonconjugative plasmids in a colony biofilm of Escherichia coli.

    abstract::We tested the possibility of nonconjugative lateral DNA transfer in a colony biofilm of mixed Escherichia coli strains. By simply coculturing a plasmid-free F(-) strain and another F(-) strain harboring a nonconjugative plasmid in a colony biofilm on antibiotic-free agar media, transformed cells were produced within 2...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2005.00072.x

    authors: Maeda S,Ito M,Ando T,Ishimoto Y,Fujisawa Y,Takahashi H,Matsuda A,Sawamura A,Kato S

    更新日期:2006-02-01 00:00:00

  • Polymerase chain reaction for identification of human and porcine spirochaetes recovered from cases of intestinal spirochaetosis.

    abstract::A polymerase chain reaction (PCR) amplification of 16S rDNA was developed to identify spirochaetes recovered from cases of intestinal spirochaetosis in humans and pigs; these bacteria belong to a distinct genetic group of spirochaetes, with the proposed name 'Anguillina coli'. The PCR incorporated a universal eubacter...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07362.x

    authors: Park NY,Chung CY,McLaren AJ,Atyeo RF,Hampson DJ

    更新日期:1995-01-15 00:00:00

  • Role of the Talaromyces marneffei (Penicillium marneffei) sakA gene in nitrosative stress response, conidiation and red pigment production.

    abstract::Stress-activated MAPK pathways are systems used to regulate the stress adaptation of most fungi. It has been shown that in Talaromyces marneffei (Penicillium marneffei), a pathogenic dimorphic fungus, the sakA gene is involved, not only in tolerance against oxidative and heat stresses, but also in playing a role in as...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw292

    authors: Nimmanee P,Tam EWT,Woo PCY,Vanittanakom P,Vanittanakom N

    更新日期:2017-04-01 00:00:00

  • Beta-glucosidase activity from the thermophilic fungus Scytalidium thermophilum is stimulated by glucose and xylose.

    abstract::An inducible mycelial beta-glucosidase from Scytalidum thermophilum was characterized. The enzyme exhibited a pI of 6.5, a carbohydrate content of 15%, and an apparent molecular mass of about 40 kDa. Optima of temperature and pH were 60 degrees C and 6.5, respectively. The enzyme was stable up to 1 h at 50 degrees C a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.09.021

    authors: Zanoelo FF,Polizeli Mde L,Terenzi HF,Jorge JA

    更新日期:2004-11-15 00:00:00

  • Influence of isolate origin and presence of various genes on biofilm formation by Enterococcus faecium.

    abstract::Enterococcus faecium, a major cause of nosocomial infections, is often isolated from conditions where biofilm is considered to be important in the establishment of infections. We investigated biofilm formation among E. faecium isolates from diverse sources and found that the occurrence and amount of biofilm formation ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12418

    authors: Almohamad S,Somarajan SR,Singh KV,Nallapareddy SR,Murray BE

    更新日期:2014-04-01 00:00:00

  • RpoS as an intermediate in RsmA-dependent regulation of secondary antifungal metabolites biosynthesis in Pseudomonas sp. M18.

    abstract::To investigate the regulatory mechanism governing antifungal metabolite biosynthesis, two kinds of global regulator genes in Pseudomonas sp. M18, an rpoS and an rsmA gene, were cloned and mutated by inserting with an aacC1 cassette, respectively. Two mutants showed the same regulatory mode with repression of phenazine...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00562.x

    authors: Ge Y,Yang S,Fang Y,Yang R,Mou D,Cui J,Wen L

    更新日期:2007-03-01 00:00:00

  • Mitochondrial DNA organisation of the mtDNA type 2b of Aspergillus tubingensis compared to the Aspergillus niger mtDNA type 1a.

    abstract::A physical and functional map of Aspergillus tubingensis mtDNA type 2b was constructed and compared to Aspergillus niger typeI a mtDNA. The gene content and order, as well as the patterns of restriction sites, were similar. Two unidentified ORFs and several repeat elements were found in the region between the ndhl and...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.10.025

    authors: Juhász A,Láday M,Gácser A,Kucsera J,Pfeiffer I,Kevei F,Hamari Z

    更新日期:2004-12-01 00:00:00

  • The role of quorum sensing in chronic cystic fibrosis Pseudomonas aeruginosa infections.

    abstract::Studies on cultured cells and in infection models have shown that cell density-dependent quorum-sensing (QS) controls many of the known virulence factors of Pseudomonas aeruginosa. However, it is less clear what role QS plays in chronic human lung infections associated with cystic fibrosis (CF). The involvement of QS ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.2008.01394.x

    authors: Winstanley C,Fothergill JL

    更新日期:2009-01-01 00:00:00

  • Salicylate uniquely induces a 27-kDa protein in tubercle bacillus.

    abstract::Salicylate was found to uniquely induce a 27-kDa protein in Mycobacterium tuberculosis complex organisms but not in Mycobacterium smegmatis or Escherichia coli. The structural analogue antitubercular para-amino-salicylate also induced the 27-kDa protein but to a somewhat lower level than salicylate. Other structural a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10843.x

    authors: Sun Z,Cheng SJ,Zhang H,Zhang Y

    更新日期:2001-09-25 00:00:00

  • Metagenome microarray for screening of fosmid clones containing specific genes.

    abstract::A critical step in the process of metagenome analysis is to screen for clones that contain specific genes among a large number of clones. To form one of the sequence-based screening tools of a metagenome library, we designed a format of microarray [metagenome microarray (MGA)] that is arrayed with fosmid library clone...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01180.x

    authors: Park SJ,Kang CH,Chae JC,Rhee SK

    更新日期:2008-07-01 00:00:00

  • Subdivision of Salmonella enteritidis PT 4 by pulsed-field gel electrophoresis: potential for epidemiological surveillance.

    abstract::Using pulsed-field gel electrophoresis (PFGE), nine profile types were identified in XbaI digests of a panel of strains of Salmonella enteritidis phage type 4 (PT 4) isolated in England and Wales between 1967 and 1992. When applied to 39 isolates from humans, chickens, poultry products and feed, strains belonging to p...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb06888.x

    authors: Powell NG,Threlfall EJ,Chart H,Rowe B

    更新日期:1994-06-01 00:00:00

  • Identification of Escherichia coli clinical isolates producing macrolide 2'-phosphotransferase by a highly sensitive detection method.

    abstract::Macrolide is inactivated with ATP plus crude extract of Escherichia coli producing macrolide 2'-phosphotransferase (MPH(2')), but not by living cells. Therefore, a convenient method for detection of MPH(2') using intact cells is needed. In this report, we determine that the modified lysozyme-DNase-RNase (LDR) method (...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13227.x

    authors: Taniguchi K,Nakamura A,Tsurubuchi K,O'Hara K,Sawai T

    更新日期:1998-10-15 00:00:00

  • Pathogenesis of Vibrio vulnificus.

    abstract::This review describes the factors which are currently recognized as being central to the virulence of the human pathogen, Vibrio vulnificus. This estuarine/marine bacterium occurs in high numbers in molluscan shellfish, primarily oysters, and its ingestion in raw oysters results in a ca. 60% mortality in those persons...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.1999.tb13570.x

    authors: Linkous DA,Oliver JD

    更新日期:1999-05-15 00:00:00

  • Segmented filamentous bacteria in human ileostomy samples after high-fiber intake.

    abstract::Segmented filamentous bacteria (SFB) are inhabitants of the small intestine of various animals, where they can be detected microscopically due to their specific morphology and intimate association with the intestinal epithelium. SFB colonize the distal part of the small intestine in a host-specific manner and affects ...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/1574-6968.12103

    authors: Jonsson H

    更新日期:2013-05-01 00:00:00

  • Correlation between the effects of fosfomycin and chloramphenicol on Escherichia coli.

    abstract::It was speculated that the increase of the UDP-GlcNAc pool observed with chloramphenicol can modulate the residual PEP:UDP-GlcNAc-enolpyruvate activity of fosfomycin-treated cells. This provided an explanation on how chloramphenicol can insure the formation of enough UDP-MurNAc-pentapeptide to sustain peptidoglycan sy...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90270-z

    authors: Mengin-Lecreulx D,van Heijenoort J

    更新日期:1990-01-01 00:00:00

  • Changes in the virulence of Mycobacterium avium after passage through embryonated hens' eggs.

    abstract::Eight-day-old embryonated hen's eggs were used as a model to study Mycobacterium avium virulence. Strains isolated from human patients caused 20-90% mortality when eggs were infected by injection of bacterial suspensions into the amniotic sac. Virulence of examined strains subsequently decreased with passage through e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09297.x

    authors: Long EG,Ewing EP Jr,Bartlett JH,Horsburgh CR Jr,Birkness KA,Yakrus MA,Newman GW,Quinn FD

    更新日期:2000-09-15 00:00:00

  • The Giardia genome project database.

    abstract::The Giardia genome project database provides an online resource for Giardia lamblia (WB strain, clone C6) genome sequence information. The database includes edited single-pass reads, the results of BLASTX searches, and details of progress towards sequencing the entire 12 million-bp Giardia genome. Pre-sorted BLASTX re...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09242.x

    authors: McArthur AG,Morrison HG,Nixon JE,Passamaneck NQ,Kim U,Hinkle G,Crocker MK,Holder ME,Farr R,Reich CI,Olsen GE,Aley SB,Adam RD,Gillin FD,Sogin ML

    更新日期:2000-08-15 00:00:00

  • Utilization of D-ribose through D-xylose transporter.

    abstract::An Escherichia coli mutant defective in high-affinity D-ribose transport is able to utilize the sugar as a sole carbon source, suggesting that other transport systems for D-ribose exist. In order to search for such transporters, transposon mutagenesis was carried out in an rbsB-negative strain containing ribokinase (r...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13054.x

    authors: Song S,Park C

    更新日期:1998-06-15 00:00:00

  • Typing of toxic strains of Clostridium difficile using DNA fingerprints generated with arbitrary polymerase chain reaction primers.

    abstract::Clostridium difficile is the causative agent for pseudomembranous colitis in humans. Toxic strains of C. difficile produce two toxins, toxin A and toxin B. A reliable and definitive method of typing the toxic strains of C. difficile is needed since nosocomial cross infection is a primary concern in hospitals and other...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90532-s

    authors: McMillin DE,Muldrow LL

    更新日期:1992-04-01 00:00:00

  • Modulation of the fecal microbiota by the intake of a Lactobacillus johnsonii La1-containing product in human volunteers.

    abstract::Lactobacillus johnsonii La1 (La1) is a probiotic strain capable of stimulating the immune system of the host and interfering with gastrointestinal pathogens. This study evaluates how the ingestion of different amounts of La1 influences the main bacterial populations of the fecal microbiota. Eight asymptomatic voluntee...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.05.045

    authors: Garrido D,Suau A,Pochart P,Cruchet S,Gotteland M

    更新日期:2005-07-15 00:00:00

  • A multiplex-PCR for the differentiation of Mycobacterium bovis and Mycobacterium tuberculosis.

    abstract::A multiplex-polymerase chain reaction (PCR) assay based on one-step amplification and detection of two different mycobacterial genomic fragments was designed for differentiation of Mycobacterium bovis and Mycobacterium tuberculosis. The oligonucleotide primers were chosen from a 500-bp genomic fragment which is well c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11322.x

    authors: Shah DH,Verma R,Bakshi CS,Singh RK

    更新日期:2002-08-27 00:00:00

  • Studies on the pathophysiological mechanism of Campylobacter jejuni-induced fluid secretion in rat ileum.

    abstract::Calcium has been reported to play an important role in regulating the intestinal electrolyte transport via Ca2+/calmodulin (CaM) and/or protein kinase C (PKC) systems. The role of Ca2+, CaM and PKC in the pathogenesis of Campylobacter jejuni-induced fluid accumulation was studied in vivo in ligated rat ileal loops. Ca...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06406.x

    authors: Kaur R,Ganguly NK,Kumar L,Walia BN

    更新日期:1993-08-01 00:00:00

  • Increased expression of biodegradative threonine dehydratase of Escherichia coli by DNA gyrase inhibitors.

    abstract::The synthesis of inducible biodegradative threonine dehydratase of Escherichia coli increased several-fold in the presence of the DNA gyrase inhibitors, nalidixic acid and coumermycin. Temperature-sensitive gyrB mutants expressed higher levels of dehydratase as compared to an isogenic gyrB+ strain. Immunoblotting expe...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(89)90362-5

    authors: Sumantran VN,Tranguch AJ,Datta P

    更新日期:1989-11-01 00:00:00

  • Assessment of the serodiagnostic potential of nine novel proteins from Mycobacterium tuberculosis.

    abstract::To identify antigens that would improve the accuracy of serological diagnosis of active tuberculosis, we cloned the genes encoding nine potentially immunogenic secreted or surface-associated proteins of Mycobacterium tuberculosis. Recombinant proteins were reacted with sera from HIV-negative individuals with extrapulm...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10615.x

    authors: Moran AJ,Treit JD,Whitney JL,Abomoelak B,Houghton R,Skeiky YA,Sampaio DP,Badaró R,Nano FE

    更新日期:2001-04-20 00:00:00

  • Construction of mini-Tn10luxABcam/Ptac-ATS and its use for developing a bacteriophage that transduces bioluminescence to Escherichia coli O157:H7.

    abstract::Mini-Tn10luxABcam/Ptac-ATS was constructed in order to develop a luciferase-transducing bacteriophage for detecting Escherichia coli O157:H7. The transposon was designed to deliver a 3.6-kb insertion that confers n-decanal-dependent bioluminescence and resistance to chloramphenicol and was constructed using mini-Tn10c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb08909.x

    authors: Waddell TE,Poppe C

    更新日期:2000-01-15 00:00:00

  • Characterization of ISR region and development of a PCR assay for rapid detection of the fish pathogen Tenacibaculum soleae.

    abstract::The aims of this work were to characterize the 16S-23S internal spacer region of the fish pathogen Tenacibaculum soleae and to develop a PCR assay for its identification and detection. All T. soleae strains tested displayed a single internal spacer region class, containing tRNA(I) (le) and tRNA(A) ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02404.x

    authors: López JR,Hamman-Khalifa AM,Navas JI,de la Herran R

    更新日期:2011-11-01 00:00:00

  • Identification of Pantoea ananatis gene encoding membrane pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase and pqqABCDEF operon essential for PQQ biosynthesis.

    abstract::Pantoea ananatis accumulates gluconate during aerobic growth in the presence of glucose. Computer analysis of the P. ananatis SC17(0) sequenced genome revealed an ORF encoding a homologue (named gcd) of the mGDH (EC 1.1.99.17) apoenzyme from Escherichia coli and a putative pyrroloquinoline quinone (PQQ) biosynthetic o...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02240.x

    authors: Andreeva IG,Golubeva LI,Kuvaeva TM,Gak ER,Katashkina JI,Mashko SV

    更新日期:2011-05-01 00:00:00

  • Serotype F double- and triple-converting phage insertionally inactivate the Staphylococcus aureus beta-toxin determinant by a common molecular mechanism.

    abstract::The precise molecular mechanism of Staphylococcus aureus beta-toxin inactivation by the serotype F triple-converting phage phi 42, phi A1 and phi A3 was investigated. Sequence analysis of the phi 42 (attP) and Staphylococcus aureus (attB) attachment sites and the left (attL) and right (attR) chromosomal/bacteriophage ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb05951.x

    authors: Carroll JD,Cafferkey MT,Coleman DC

    更新日期:1993-01-15 00:00:00