Abstract:
:We isolated nickel-resistant bacterium from soil in order to identify a novel nickel resistance determinant. Using 16S rRNA gene sequencing, an isolate was identified as Enterobacter sp. Ni15. This species showed a medium-level (resistant to up to 10 mM) nickel resistance in nutrient-rich media. Enterobacter sp. Ni15 has a novel plasmid, pNi15, and an increased nickel resistance to Escherichia coli DH5alphain trans. To isolate the nickel resistance gene from pNi15, the plasmid was digested with XbaI and its fragments were cloned into pBluescriptIISK(+). The clones were transferred into E. coli DH5alpha. The nickel resistance of the clones was then assayed. From these results, a pNi15100 isolate containing a 5,328 bp XbaI fragment of pNi15 was identified and sequenced. The E. coli DH5alpha harboring the pNi15100 showed a resistance to up to 7 mM nickel. Using a subcloning analysis, we were able to identify the novel nickel resistance determinant: the nrp gene encoding the putative proteins NrpA and NrpB.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Lee YK,Chang HH,Lee HJ,Park H,Lee KH,Joe MHdoi
10.1111/j.1574-6968.2006.00130.xkeywords:
subject
Has Abstractpub_date
2006-04-01 00:00:00pages
177-81issue
2eissn
0378-1097issn
1574-6968pii
FML130journal_volume
257pub_type
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journal_title:FEMS microbiology letters
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