Isolation of a novel plasmid, pNi15, from Enterobacter sp. Ni15 containing a nickel resistance gene.


:We isolated nickel-resistant bacterium from soil in order to identify a novel nickel resistance determinant. Using 16S rRNA gene sequencing, an isolate was identified as Enterobacter sp. Ni15. This species showed a medium-level (resistant to up to 10 mM) nickel resistance in nutrient-rich media. Enterobacter sp. Ni15 has a novel plasmid, pNi15, and an increased nickel resistance to Escherichia coli DH5alphain trans. To isolate the nickel resistance gene from pNi15, the plasmid was digested with XbaI and its fragments were cloned into pBluescriptIISK(+). The clones were transferred into E. coli DH5alpha. The nickel resistance of the clones was then assayed. From these results, a pNi15100 isolate containing a 5,328 bp XbaI fragment of pNi15 was identified and sequenced. The E. coli DH5alpha harboring the pNi15100 showed a resistance to up to 7 mM nickel. Using a subcloning analysis, we were able to identify the novel nickel resistance determinant: the nrp gene encoding the putative proteins NrpA and NrpB.


FEMS Microbiol Lett


Lee YK,Chang HH,Lee HJ,Park H,Lee KH,Joe MH





Has Abstract


2006-04-01 00:00:00














  • Successful enrichment of low-abundant comammox Nitrospira from nitrifying granules under ammonia-limited conditions.

    abstract::In artificial engineered systems, nitrification is a key reaction that accounts for the removal of biological nitrogen. Recently, a single microbe capable of oxidizing ammonia to nitrate, known as a complete ammonia oxidizer (comammox), has been discovered. Although the abundance and diversity of comammox Nitrospira i...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fujitani H,Nomachi M,Takahashi Y,Hasebe Y,Eguchi M,Tsuneda S

    更新日期:2020-01-01 00:00:00

  • Hydrocarbon utilization within a diesel-degrading bacterial consortium.

    abstract::Diesel fuel is a common environmental pollutant comprised of a large number of both aromatic and aliphatic hydrocarbons. The microbial degradation of individual hydrocarbons has been well characterized, however, the community dynamics within a system degrading a complex pollutant such as diesel fuel are still poorly u...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ciric L,Philp JC,Whiteley AS

    更新日期:2010-02-01 00:00:00

  • CpaA a novel protease from Acinetobacter baumannii clinical isolates deregulates blood coagulation.

    abstract::Acinetobacter baumannii is an important nosocomial pathogen that displays high antibiotic resistance. It causes a variety of infections including pneumonias and sepsis which may result in disseminated intravascular coagulation. In this work, we identify and characterize a novel secreted, zinc-dependent, metallo-endope...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Tilley D,Law R,Warren S,Samis JA,Kumar A

    更新日期:2014-07-01 00:00:00

  • Occurrence of aminoglycoside-modifying enzymes among isolates of Escherichia coli exhibiting high levels of aminoglycoside resistance isolated from Korean cattle farms.

    abstract::This study investigated 247 Escherichia coli isolates collected from four cattle farms to characterize aminoglycoside-modifying enzyme (AME) genes, their plasmid replicons and transferability. Out of 247 isolates a high number of isolates (total 202; 81.78%) were found to be resistant to various antibiotics by disc di...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Belaynehe KM,Shin SW,Hong-Tae P,Yoo HS

    更新日期:2017-08-01 00:00:00

  • Similar subunit architecture of archaeal and eukaryal RNA polymerases.

    abstract::Protein interactions among RNA polymerase small subunits from the archaeon Methanococcus jannaschii were investigated using affinity pulldown assays in pairwise and higher-order combinations. In the most extensive study of archaeal RNA polymerase subunit interactions to date, including 37 pairs of proteins, 10 ternary...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Best AA,Olsen GJ

    更新日期:2001-02-05 00:00:00

  • Magnaporthe oryzae endopolygalacturonase homolog correlates with density-dependent conidial germination.

    abstract::Endopolygalacturonases (endoPGs) of some phytopathogens are virulent factors for dicots. To investigate the function of the endoPG of Magnaporthe oryzae, a disruption mutant of MGG_08938, the homolog of endoPG found in the genome database of this fungus, was generated. The pathogenicity, mycelial growth, and appressor...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mori T,Jung HY,Maejima K,Hirata H,Himeno M,Hamamoto H,Namba S

    更新日期:2008-03-01 00:00:00

  • A unique and common restriction fragment pattern of the nucleotide sequences homologous to the genome of vf33, a filamentous bacteriophage, in pandemic strains of Vibrio parahaemolyticus O3:K6 O4:K68, and O1:K untypeable.

    abstract::Vf33 is a filamentous bacteriophage isolated from Vibrio parahaemolyticus. We performed Southern blot hybridization analysis to examine the distribution of Vf33-related genetic elements in the pandemic strains (O3:K6 strains isolated between 1995 and 1997, O4:K68 and O1:K untypeable strains isolated between 1997 and 1...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Chang B,Yoshida S,Miyamoto H,Ogawa M,Horikawa K,Ogata K,Nishibuchi M,Taniguchi H

    更新日期:2000-11-15 00:00:00

  • Zinc content of the Bacillus anthracis lethal factor.

    abstract::We present evidence that the anthrax toxin lethal factor binds multiple zinc atoms. Results from atomic adsorption spectroscopy indicate that lethal factor contains approximately three zinc atoms per toxin molecule. Lethal factor treated with EDTA and o-phenanthroline contained a similar number of zinc atoms, indicati...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kochi SK,Schiavo G,Mock M,Montecucco C

    更新日期:1994-12-15 00:00:00

  • Acquisition of plasmin activity and induction of arachidonic acid release by Streptococcus suis in contact with human brain microvascular endothelial cells.

    abstract::Infections caused by Streptococcus suis, a major swine pathogen, include meningitis, arthritis, pneumonia and septicaemia. In this study, we investigated interactions that may occur between human brain microvascular endothelial cells (HBMEC), the main constituent of the blood-brain barrier, and S. suis. We show that S...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jobin MC,Fortin J,Willson PJ,Gottschalk M,Grenier D

    更新日期:2005-11-01 00:00:00

  • Growth, incidence and activities of dissimilatory sulfate-reducing bacteria in the human oral cavity.

    abstract::Viable counts and activities of sulfate-reducing bacteria were determined in the oral cavities of 12 healthy volunteers. Of these, 10 harboured viable sulfate-reducing bacteria populations. Six separate sites were sampled: the posterior tongue, anterior tongue, mid buccal mucosa, vestibular mucosa, supragingival plaqu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Willis CL,Gibson GR,Allison C,Macfarlane S,Holt JS

    更新日期:1995-06-15 00:00:00

  • Molecular cloning of a light-inducible gene (lipA) encoding a novel pilin from Arthrobacter photogonimos.

    abstract::Development of pili on cells of Arthrobacter photogonimos is induced by photo-oxidative conditions. The nucleotide sequence was determined of a light-inducible gene (lipA) that encodes the precursor of a light-inducible pilin (designated LIP), a polypeptide of 212 amino acids. The N-terminal leader peptide includes a ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Yang HS,Hoober JK

    更新日期:1998-06-01 00:00:00

  • Aberrant gene for E1 Tor hemolysin from Vibrio cholerae non-O1, N037.

    abstract::Vibrio cholerae non-O1 strain N037 produced a hemolysin (NO37-Hly) which was antigenically similar to E1 Tor hemolysin (E1 Tor-Hly) but different in molecular size, hemolytic activity, and glucose binding capacity. In the gene encoding NO37-Hly, a 4-bp insertion into the structural gene for E1 Tor-Hly (hlyA) was found...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Honma Y,Yamamoto K,Iwanaga M

    更新日期:1995-11-01 00:00:00

  • Light-activated adenyl cyclase from Trichoderma viride.

    abstract::The effect of light on adenyl cyclase (E.C. and 3':5'-cyclic-AMP-phosphodiesterase (E.C. activity of Trichoderma viride was investigated. Adenyl cyclase proved to be a membrane-associated enzyme, requiring Mn2+ and was activated by light. In contrast, 3':5'-cyclic-AMP-phosphodiesterase showed no lig...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kolarova N,Haplová J,Gresík M

    更新日期:1992-06-15 00:00:00

  • Roseovarius sp. strain 217: aerobic taurine dissimilation via acetate kinase and acetate-CoA ligase.

    abstract::The genome sequence of Roseovarius sp. strain 217 indicated that many pathway enzymes found in other organisms for the degradation of taurine are represented, but that a novel, apparently energy-dependent pathway is involved in the conversion of acetyl phosphate to acetyl CoA. Thus, an ABC transporter for taurine coul...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Baldock MI,Denger K,Smits TH,Cook AM

    更新日期:2007-06-01 00:00:00

  • Enzymatic versus spontaneous S-methyl thioester synthesis in Geotrichum candidum.

    abstract::The synthesis of short chain S-methyl thioesters was investigated in Geotrichum candidum strain GcG. The results indicated the involvement of an enzymatic reaction in this microorganism that led to the synthesis of S-methyl thioacetate (MTA) when methanethiol and acetyl-CoA were used as substrates. MTA was generated f...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Helinck S,Spinnler HE,Parayre S,Dame-Cahagne M,Bonnarme P

    更新日期:2000-12-15 00:00:00

  • Linkage between cellular adherence and biofilm formation in Escherichia coli O157:H7 EDL933.

    abstract::During the establishment of Escherichia coli O157:H7 infection, its capacity to adhere to host intestinal epithelial cells is the critical first step in pathogenesis. It also has the capability to form biofilms, and because both are surface activities, we sought to gain insight into a potential linkage between biofilm...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Puttamreddy S,Minion FC

    更新日期:2011-02-01 00:00:00

  • Extraction and characterization of lipopolysaccharide from Pseudomonas pseudomallei.

    abstract::The best yield of lipopolysaccharide (LPS) of Pseudomonas pseudomallei GIFU 12046 was obtained by extraction of defatted cells by phenol/chloroform/petroleum ether. The LPS showed a smooth character on SDS-polyacrylamide gel electrophoresis and contained D-glucose, L-glycero-D-manno-heptose, and D-glucosamine as the m...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kawahara K,Dejsirilert S,Danbara H,Ezaki T

    更新日期:1992-09-15 00:00:00

  • The presence of capsular polysaccharide in mesophilic Aeromonas hydrophila serotypes O:11 and O:34.

    abstract::Mesophilic Aeromonas hydrophila from serotypes O:11 and O:34 grown in a glucose-rich medium produce a capsule that can be seen under light and electron microscopy. The purified capsular polysaccharide has a composition qualitatively similar for strains O:11 and O:34, but quantitatively different. The capsular polysacc...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Martínez MJ,Simon-Pujol D,Congregado F,Merino S,Rubires X,Tomás JM

    更新日期:1995-04-15 00:00:00

  • Food grade zinc oxide bulk particle composite can replace the toxic zinc oxide nano composite towards the control of pathogenic and spoilage bacteria.

    abstract::ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite has potential biomedical and food applications due to its better antimicrobial activity. However, the presence of nano-metal-oxide in the composite makes the material unsuitable for any food applications. Moreover, the cost involved in the preparation of Zinc Oxide-Nano-...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Visnuvinayagam S,Murthy LN,Parvathy U,Jeyakumari A,Sivaraman GK,Karthikeyan D

    更新日期:2020-12-15 00:00:00

  • Characteristics, transcriptional patterns and possible physiological significance of glycoside hydrolase family 16 members in Coprinopsis cinerea.

    abstract::The glycoside hydrolase (GH) 16 family of Coprinopsis cinerea includes 15 members distributed in four subgroups (A1, A2, B and D) by phylogenetic analysis. The expression patterns match well with the requirement of wall-softening in the germination of basidiospores, hyphal growth and branching, primordium formation, s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kang L,Zhu Y,Bai Y,Yuan S

    更新日期:2019-04-01 00:00:00

  • Gel electrophoretic analysis of penicillin-binding proteins of coagulase negative staphylococci.

    abstract::We analyzed gel electrophoretic banding patterns of penicillin-binding proteins (PBPs) of 16 type strains of coagulase-negative staphylococci (CNS). S. epidermidis, S. haemolyticus, S. saprophyticus, S. hominis, S. xylosus, S. simulans, S. warneri, S. capitis, S. saccharolyticus, S. auricularis, S. caseolyticus, S. ga...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kanda K,Hiramatsu K,Yokota T

    更新日期:1990-10-01 00:00:00

  • A new subtype of the metalloprotease toxin gene and the incidence of the three bft subtypes among Bacteroides fragilis isolates in Japan.

    abstract::The bft gene encoding Bacteroides fragilis toxin (BFT) has been devided into two subtypes, bft-1 and bft-2. We found a novel subtype by sequencing a segment of the bft gene from 64 enterotoxigenic B. fragilis (ETBF) strains isolated in Japan. The 1548-bp nucleotide sequences of the new bft, the bft-1, and bft-2 genes ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kato N,Liu CX,Kato H,Watanabe K,Tanaka Y,Yamamoto T,Suzuki K,Ueno K

    更新日期:2000-01-01 00:00:00

  • Chemical and electroporated transformation of Edwardsiella ictaluri using three different plasmids.

    abstract::Transfer of DNA by conjugation has been the method generally used for genetic manipulation of Edwardsiella ictaluri because, previously, attempts to transform E. ictaluri by the uptake of naked DNA have apparently failed. We report here the successful transformation of seven strains of E. ictaluri using electroporatio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Russo R,Panangala VS,Wood RR,Klesius PH

    更新日期:2009-09-01 00:00:00

  • Characterization of DegU-dependent expression of bpr in Bacillus subtilis.

    abstract::The response regulator DegU and its cognate histidine kinase DegS constitute a two-component system in the Gram-positive soil bacterium Bacillus subtilis. The phosphorylated form of DegU is known to activate transcription of more than 120 genes in B. subtilis, including the bpr gene encoding bacillopeptidase F. To cha...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Tsukahara K,Ogura M

    更新日期:2008-03-01 00:00:00

  • A multiplex-PCR for the differentiation of Mycobacterium bovis and Mycobacterium tuberculosis.

    abstract::A multiplex-polymerase chain reaction (PCR) assay based on one-step amplification and detection of two different mycobacterial genomic fragments was designed for differentiation of Mycobacterium bovis and Mycobacterium tuberculosis. The oligonucleotide primers were chosen from a 500-bp genomic fragment which is well c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shah DH,Verma R,Bakshi CS,Singh RK

    更新日期:2002-08-27 00:00:00

  • Growth inhibition of Prevotella ruminicola by protamine.

    abstract::Growth of Prevotella ruminicola strains B(1)4 (subsp. brevis) and D31d (subsp. ruminicola), was inhibited by protamine, a polycationic, low molecular mass protein. Results showed that protamine has a bacteriocidal effect when present in concentrations exceeding 30 micrograms ml-1. Protamine exerted its toxic effects b...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Madeira HM,Morrison M

    更新日期:1997-05-01 00:00:00

  • Mycoplasma gallisepticum rpoA gene cluster.

    abstract::Two conservative gene clusters, the S10 ribosomal protein region and one (of the two) set of rRNA genes, were split in a genome crossover rearrangement event in Mycoplasma gallisepticum. As a result of the rearrangement the major part of the S10 ribosomal protein cluster is located upstream of genes for 23S-5S rRNA (r...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Skamrov A,Feoktistova E,Goldman M,Beabealashvilli R

    更新日期:2002-03-05 00:00:00

  • Inhibition of aerobic respiration and dissimilatory perchlorate reduction using cyanide.

    abstract::The effect of low concentrations of cyanide on dissimilatory perchlorate and chlorate reduction and aerobic respiration was examined using pure cultures of Azospira sp. KJ. Cyanide at a concentration of 38 microM inhibited cell growth on perchlorate, chlorate and molecular oxygen, but it did not inhibit the activity o...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Song Y,Logan BE

    更新日期:2004-10-15 00:00:00

  • Physicochemical modification of the excretion product of Saccharomyces cerevisiae killer strains results in fungicidal activity against Candida albicans and Tricophyton mentagrophytes.

    abstract::It is known that certain yeast strains, so called 'killers', can produce and excrete proteinaceous toxins that can induce death of other sensitive strains. We obtained a stable fungicidal factor (SKF) through concentration and stabilization of the excretion product of certain killer strains of Saccharomyces cerevisiae...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Bracesco N,Salvo VA,Carrau FM,Nunes E

    更新日期:2006-03-01 00:00:00

  • Two methods for the genetic differentiation of Lactococcus lactis ssp. lactis and cremoris based on differences in the 16S rRNA gene sequence.

    abstract::The 16S ribosomal RNA gene sequences of Lactococcus lactis ssp. lactis and ssp. cremoris differ by 9-10 bp (depending on strain), within the first 200 bp of the sequence. These differences were used to develop two methods of genetically differentiating lactis and cremoris strains. Primers to conserved sequences in the...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ward LJ,Brown JC,Davey GP

    更新日期:1998-09-01 00:00:00